Rebounder 運動負荷後 恢復期의 心肺機能의 變化

裵玉錫,黃樹寬,金亨鎭,朱永恩 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.2

20∼22歲의 男子大學生 61名을 硏究對象으로 하여 選手群(21名)과 非選手群(40名)으로 區分하였다 運動負荷는 rebouner에 垂直뛰기를 一分間에 80回程度로 3分동안 實施했으며, 運動後 恢復期 1, 3, 5, 10 및 20分에서 心搏數, 血壓, 呼吸數의 恢復樣相을 觀察하였다. 이로써 체력단속이 恢復期의 心肺機能에 미치는 影響을 分析하고 나아가서 恢復期의 心肺反應이 體力을 評價하는 指標가 될 것인가를 알고자 試圖한 本 硏究結果를 要約하면 다음과 같다. 安靜狀態의 心搏數는 選手群이 非選手群에 比해서 有意하게 낮았으며 血壓 및 呼吸數는 別 差異가 없었다. 運動負荷後 心搏數의 恢復은 選手群은 恢復 1分에 86.4±3.22回로서 非選手群의 118.0±2.81回에 比해 有意하게 낮았고, 兩群 모두 恢復初期에는 急速한 恢復을 보였으며 選手群이 非選手群에 比해 繼續有意하게 낮은 값으로 빠른 速度로 恢復되었다. 運動負荷後 收縮期血壓의 恢復樣相은 選手群이 非選手群보다 繼續 낮은 값으로 恢復되었으며, 特히 恢復 1分에 選手群은 161.2±1.78mmHg로 非選手群의 174.1±3.41mmHg에 比해 有意하게 낮았다. 兩群 모두 恢復初期에 急速히 恢復하여 恢復10分에는 安靜狀態로 恢復되었다. 그리고, 擴張期血壓은 非選手群은 期動後 安靜狀態보다 有意하게 높았으며, 選手群은 오히려 安靜狀態보다 낮았다. 平均動脈壓은 恢復初期에 選手群이 非選手群보다 높은 값으로 恢復되었으나 恢復10分부터는 거의 같은 값으로 恢復되었다. 脈壓은 選手群이 非選手群에 比해 繼續 有意하게 낮은 값으로 恢復되었다. 運動負荷後 呼吸數는 恢復 1分에 選手群에서는 27.1±1.23回로 非選手群의 35.5±1.17回에 比해 有意하게 낮았고, 兩群 모두 恢復 1,3分에 急速히 恢復되었으며, 選手群이 非選手群에 比해 繼續 有意하게 낮은 값으로 恢復되었다. 本硏究結果를 綜合하면 rebounder 運動負荷後 選手群의 心搏數, 血壓 및 呼吸數가 非選手群에 比해 有意하게 낮았으며, 그 恢復速度도 빨랐다. 특히 心搏數는 選手群과 非選手群 사이에 恢復初期부터 恢復 20分까지 繼續 有意한 差異를 나타낸 점은 體力을 客觀的으로 評價하는 重要한 資料가 될 것으로 思料되는 바이다. Sixty-one male subjects aged between 20-22 years were divided into the athletic and non-athletic groups. Exercise was performed as vertical jump on a rebounder at 80 times per min for 3min. The heart rate, blood pressure, and respiratory rate were measured at 1, 3, 5, 10, and 20min after exercise in an effort to analyze the effect of the physical training on the cardiopulmonary function during the recovery period, and to find out if the cardiopulmonary responses during recovery period can be an index of physical fitness. The results are summarized as follows. In resting state, the heart rate was significantly lower in athletes than in non-athletes, while blood pressure and respiratory rate showed no significant difference between the two groups. The heart rate at 1min after the exercise was 86.4±3.22 in athletes which was significantly lower than 118.5±7.85 in non-athletes. The heart rate at the initial stage after exercise showed a rapid recovery in both groups. In athletes, it showed a rapid recovery to reach a significantly lower level than that of non-athletes. The systolic pressure was lower in athletes than in non-athletes throughout the recovery period. In particular, systolic pressure at 1min after the exercise was 161.2±1.78 mmHg in athletes which was significantly lower than 174.1±3.41mmHg in non-athletes. Systolic pressure at initial stage after the exercise showed a rapid recovery, and the resting value was retored in 10 min in both groups. Diastolic pressure during the recovery period was significantly higher in non-athletes and lower in athletes than the resting level.

고등학교 운동선수에서 최대하 Treadmill 운동시 심폐기능의 변화와 산소섭취량의 추산법

배옥석,김형진,박재식,주영은 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.3

고등학교 운동선수에서 최대하 treadmill 운동시험시 심폐기능의 변화를 구명하고 운동부하, 운동시간 및 심박수로부터 산소섭취량을 추산할 수 있는 회귀방정식을 유도하고자 15∼18세의 남자 고등학생 운동선수 14명과 비운동선수 16명을 대상으로 하여 Bruce법에 따라 treadmill 상에서 최대 운동능력의 80%에 해당하는 최대하운동을 실시하고 운동전, 운동중 및 운동후에 심박수, 혈압, 분시환기량, 탄산가스생산량 및 산소섭취량을 측정하였으며 운동 전후에 폐환기역학 검사를 시행하였다. 심박수는 선수군에서 안정시의 71.9±1.3회로부터 운동중 178.0±2.0회까지 증가하여 최대심박수의 81.2±2.3%에 달하였고 비선수군에서는 안정시의 81.8±2.4회로부터 182.6±1.1회까지 증가하여 최대심박수의 79.6±2.0%에 달하였다. 선수군의 심박수는 비선수군에 비해 전 실험기간에 걸쳐 유의(P<0.01)하게 낮은 값을 유지하였다. 분시환기량은 선수군에서 안정시의 5.1±0.6 L/min로부터 운동중 63.0 L/min까지 증가하였고 비선수군에서는 안정시의 5.2±0.5 L/min로부터 51.2 L/min 까지 증가하였으며 양군 사이에는 유의한 차이가 없었다. 산소섭취량은 선수군에서 안정시 1.0±0.4 ㎖/㎏/min로부터 운동중 32.6 ㎖/㎏/min까지 증가하여 최대산소섭취량의 72.9%에 달했으며 비선수군에서는 안정시 1.4±0.3 ㎖/㎏/min로부터 28.9 ㎖/㎏/min까지 증가하여 최대산소섭취량의 70.3%에 달하였다. 선수군의 산소섭취량은 비선수군에 비해 계속 낮은 경향을 보였다. Treadmill 속도 및 경사도와 산소섭취량 간의 중회귀분석 결과 중상관계수 0.9998로서 높은 상관관계를 나타내었다. Bruce법에 따른 운동시간과 산소섭취량 간의 회귀분석 결과 1차함수 회귀식에 대한 상관계수는 0.999였으나 3차함수 회귀식에 대한 상관계수는 0.99999로서 극히 높은 상관관계를 나타내었다. 심박수와 산소섭취량 간에는 상관계수 0.998 및 0.992로서 대체로 높은 직선적 상관관계를 나타내었다. 폐기능검사 결과 안정시 FVC, FEF_(0.2-1.2L), FEF_25% 및 MVV는 선수군에서 비선수군에 비해 유의(P<0.05)하게 높았다. 운동후 폐기능검사 성적은 비선수군에서는 안정시보다 저하된 경향을 보인데 비해 선수군에서는 향상된 경향을 보였다. The cardiorespiratory function was evaluated during and after a submaximal treadmill exercise and some regression equations were derived for estimation of oxygen uptake from exercise load, exercise time and the heart rate. A total of 30 high school boys, of whom 14 were athletic and 16 non-athletic, performed a submaximal treadmill exercise according to the Bruce protocol to the point when 80% of their maximum exercising capacity were reached. The heart rate, blood pressure, minute ventilation, CO_2 production and O_2 uptake were measured before, during and after exercise, and the pulmonary function test was performed before and after exercise. Heart rate was increased from 71.9±1.3 beats/min at rest to 178.0±2.0 during exercise which was 81.2±2.3% of the maximum heart rate in athletes, and from 81.8±2.4 beats/min at rest to 182.6±1.1 which was 79.6±2.0% of its maximum in non-athletes. Athletes showed significantly (P<0.01) lower heart rate than non-athletes throughout the experimental period. Minute ventilation was increased from 5.1±0.6 L/min at rest to 63.0 in athletes and from 5.2±0.5 L/min to 51.2 in non-athletes. Athletes and non-athletes showed no significant difference. Oxygen uptake was increased from 1.0±0.4 ㎖/㎏/min at rest to 32.6 which was 72.9% of maximum oxygen uptake in athletes, and from 1.4±0.3 ㎖/㎏/min to 28.9 which was 70.3% of the maximum in non-athletes. Athletes showed consistently lower oxygen uptake than non-athletes. Multiple regression analysis between treadmill speed and grade and the oxygen uptake showed a high correlation coefficient of 0.9998. A first order regression analysis between exercise time and oxygen uptake showed a correlation coefficient of 0.999, while a third order regression analysis showed an extremely high correlation coefficient of 0.99999. The heart rate and oxygen uptake showed a linear regression with a reasonably high correlation coefficient of 0.998 and 0.992. The pulmonary function test revealed significantly(P<0.05) higher FVC, FEF_(0.2-1.2L), FEF_25% and MVV at rest in athletes than in non-athletes. After exercise, athletes showed enhanced pulmonary function compared with the resting state while non-athletes showed more or less decreased.

충수염의 임상적 고찰과 초음파의 유용성

박정배,양혁준,김형수,황성연,박철완,이근 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

Appendicitis is the most common acute abdomen in Emergency Medical Center & outpatient department. But sometimes, it is difficult for us to diagnose because of clinically variable symptoms. We reviewed the ultrasonographic findings and analyse 521 cases of appendectomy patients about its distribution of sex and age, seasonal prevalence, clinical symptom and signs, laboratory findings, duration from onset of symptom to arrival at hospital, duration from arrival at hospital to operation, vital signs, operative results, and associated diseases, which were treated at the department of General Surgery & Emergency Medical Center in Chung Ang Gil Hospital from July, 1994 to June, 1995. The results were as follows : 1. The most common age group was second decade. And male to female ratio was 1.43:1. Seasonal variation was not significant. 2. Of 521 cases, 141 cases(27.1%) visited the hospital within 24 hours from the onset of symptom, and 259 cases(49.7%) visited between 24 to 48 hours. 3. In duration from arrival to operation, the cases within 6 hours were 263 (50.5%), the cases between 6 and 12 hours were 131(25..1%). 4. In systolic blood pressure, cases below 90mmHg were 19(3.6%), cases above 160mmHg were 23(4.4%). Below 60mmHg diastolic pressure were 55 cases(10.6%), cases above 110mmHg were 9(1.7%). In pulse rate, above 90 rate/min were 171(32.8%). 5. On admission leukocytosis was found in 413 cases(79.3%) and 342 cases of them were pathologic appendicitis. 6. Ultrasoundgraphy was performed in the 87 cases, but 23 cases were suggested appendicitis. Among 23 cases, 22(95.7%) revealed pathologic appendicitis at ooperation and labortatory examination. Of the 64 cases which were normal on ultrasonography, only 2 cases(3.1%) were proved to be appendicitis. 7. The results of 496 operations without ultrasonography were appendicitis in 390 cases(78.6%), mesenteric lymphadenopathy 36(7.3%), pelvic inflammatory disease 28(5.6%), fecalith 15(3.0%), terminal ileitis 8(1.6%), diverticulitis 7(1.4%), urinary tract stones 5(1.0%), cecal cancer 2 cases, right ovarian tumor 2 cases, tubo-ovarian abscess 2 cases, grater omentum internal hernia 1 case. Our results showed that the sensitivity of ultrasonohraphy was 95.8% and the specificity 98.4% in the diagnosis of appendicitis, It suggests that the ultrasonography is very helpful in the diagnosis & differential diagnosis of acute appeendiditis.

경사진 트레드밀에서 후방보행운동을 통한 넙다리네갈래근 강화와 요통의 관계

박주완,김경,김형욱,김재우,배솔희,서정표,이정구,정하영 대구대학교 특수교육재활과학연구소 2010 再活科學硏究 Vol.28 No.1

목적: 본 연구에서는 경사진 트레드밀에서의 후방 보행 훈련을 통한 넙다리네갈래근의 강화가 요통 환자의 치료에 미치는 영향을 알아보고자 하였다. 대상: 만성 요통을 호소하는 16명의 환자를 대상으로 연구를 실시 하였다. 방법: 트레드밀을 10° 경사로 맞춰놓은 상태에서 대상자들은 하루 20분, 일주일에 3회의 빈도로 2주 동안 훈련을 하였다. 표면 근전도를 가지고 넓다리 곧은근, 안쪽 넓은근, 가쪽 넓은근, 척추 세움근의 %MVIC를 측정하였고, KODI와 VAS를 통해 대상자들의 통증정도를 측정하였다. 결과: 넓다리 곧은근은 훈련전에 비해 훈련을 하고 난 후 상대적으로 높은 근전도를 보였고, 통계적으로 유의하였다(p<.05). 안쪽 넓은근과 가쪽 넓은근 또한 높은 근전도율을 보였지만 통계적으로 유의하지는 않았다. 척추 세움근은 훈련전에 비해 훈련을 하고 난 후 높은 근전도율을 보여주었고, 통계적으로 유의하였다(p<.05). VAS와 KODI의 평균값은 명백하게 감소되었고, 통계적으로 유의하였다(p<.05). 결론: 이상의 결론을 종합하면, 후방보행을 통한 넙다리네갈래근의 강화는 요통의 감소에 효과적이라고 사료되며, 더불어 넙다리네갈래근으로 접근하여 요통을 경감하는 중재방법이 효과적인 치료 방법이 될 수 있다는 것을 의미한다. Purpose: This study was to investigate the influence of strengthening the Quadriceps femoris through backward walking treadmill training in the patients who have lower back pain. Subjects: Sixteen subjects with chronic lower back pain were included. Method: In the training, the treadmill was set to have a inclination of 10 degrees, and the subjects were trained for 20 minutes a day, 3 times a week, for 2 weeks. %MVIC(muscle power) of rectus femoris, vastus medialis oblique, vastus lateralis and erctor spinae were measured with surface EMG, and KODI and VAS were used to measure the subject's level of pain. Results: Quadriceps femoris showed relatively higher average electromyogram rates than that before training(p<.05). Vastus medialis oblique and vastus lateralis also showed higher average electromyogram rates, but no significant change in them was noted. Erector spinae showed higher average electromyogram rates than that before training(p<.05). The average points of VAS and KODI clearly decreased(p<.05). Conclusion: Strengthening Quadriceps femoris seems to be efficient for relieving lower back pain in this study.

정신분열병과 Neurotensin 수용체 유전자 다형성의 연합 연구

이유상,김형배,한진희,채영규,이정식,이혜순,주연호,김형섭,최인근,양병환 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6

연구목적: Neurotensin(NT)은 NT수용체와 결합하여 그 효과를 나타내는 neuromodulator 혹은 neurotransmitter로서 대뇌에서 도파민의 분비를 조절하는데 중요한 역할을 한다. 근래의 연구에 의하면 NT와 그 수용체는 대뇌에서 항정신병 약물의 효과를 매개하는 것으로 생각되고 있으며 약물치료를 받지 않은 정신분열병 환자의 뇌척수액에서 NT의 양이 적으로 보고되고 있어 이들은 정신분열병과 깊은 관련을 가지고 있을 것으로 추정된다. 최근 NT수용체의 유전자의 3`인접영역에서 정보가치가 높은 4 염기반복 다형성이 발견되어 이를 유전 표지자로 하여 정신분열병과의 연합을 알아보았다. 방 법: 서로 혈연관계에 있지 않은 정신분열병 환자 120명(남자 91명, 여자 29명)과 정상 대조군 106명(남자 84명, 여자 22명)을 대상으로 하였다. PANSS를 사용하여 양성 및 음성을 알아보았다. 말초혈액에서 DNA를 분리한 후에 중합효소연쇄반응을 사용하여 3`인접영역에 있는 4 염기 반복 다형성을 증폭하였고 silver staining한 후에 유전자형을 관찰하였다. chi-square 검증과 Bonferroni`s correction을 사용하여 환자군과 정상 대조군간의 대립유전자 빈도의 차이를 알아보았다. 또한 양성 및 음성 환자군으로 나누어 차이를 알아보았다. 결 과: 총 23개의 대립유전자가 관찰되었으며, 399bp의 대립유전자(A10)의 빈도가 환자군보다 정상대조군에서 통계적으로 유의하게 높았다(χ²=16.49, df=1, p<0.001). 음성 정신분열병 환자군과 정상대조군 사이의 비교에서는 정상대조군의 A10의 빈도가 환자군보다 유의하게 높았다(χ²=21.33, df=1, p<0.001). 성별 비교에서 남자 정신분열병 환자군은 대조군에 비하여 A10의 분포가 유의하게 적었다. (χ²=13.71, df=1, p<0.001) 결 론: NT 수용체 유전자와 정신분열병사이에 음성연합이 관찰되었다. NT 수용체 유전자가 일부 정신분열병의 발병과정에서 확실하지는 않지만 어떤 종류의 보호기능을 할 수도 있다는 것을 암시한다. Objectives: Neurotensin(NT), of which functions are evoked by its interaction with neurotensin receptors(NTR), coexists with mesolimbic dopamine and regulates endogenous dopamine release. Recent studies have shown that NT with NTR exerts neuroleptic-like activity within the central nervous system and may play an important role in the pathogenesis and in the treatment of schizophrenia. We have examined the gentic association between schizophrenia and tetranucleotide repeat polymorphism in the 3-flanking region of the NTR gene to investigate the possible contribution of the NTR gene to the schizophrenia susceptibility. Methods: Among 23 alleles identified, the subjects were 120 patients(male 91, female 29)with schizophrenia and 106 normal healthy controls(male 84, female 22). They were unrelated native Korean. PANSS was used to determine positive or negative subgroup in the schizophrenic patients. Using polymerase chain reaction and polyacrylamide gel electrophoresis, tetranucleotide repeat polymorphism(CCTT and CTT) in the 3`-flanking region of NTR gene was observed. For a comparison of NTR gene`s allelic frequencies between patients with schizophrenia and normal healthy controls, chi-square test and Bonferroni`s correction was performed. Results: The frequency of A10 allele(base pair size=399)was significantly higher in normal healthy controls than schizophrenia(χ²=16.4902, df=1, p<.000). In the comparison between schizophrenic patients with negative symptoms and normal controls, the frequency of A10 allele was significantly higher in normal healthy control subjects than patients with schizophrenia(χ²=21.33, df=1, p<0.001). In the case of male, the frequency of A10 allele of schizophrenia was significantly higher than normal controls(χ²=13.71, df=1, p<0.001). Conclusions: NTR gene was negatively associated with schizophrenia. NTR gene`s tetranucleotide repeat polymorphism may provide some protective function against schizophrenia.

기능성 Catechol-O-Methyltransferase 유전자 다형성과 한국인 정신분열병의 관련성

박태원,홍영준,박임순,김형섭,최인미,배수영,윤경식,강대희 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : Catechol-O-Methyltransferase(COMT)는 카테콜아민 신경전달물질의 불활성화를 담당하는 효소로서, 정신분열병과 관련된 후보 유전자로서 알려져 왔다. 최근, 기능성 COMT유전자 다형성과 정신분열병의 관련성이 제기되었는데, 한국인 정신분열병에서도 이러한 관련성이 있는지를 살피기 위해 본 연구를 시행하였다. 방 법 : 정신분열병으로 진단된 환자군 103명과 성별 및 연령별로 1:1 짝짓기 대조군 사이에 유전자형과 대립유전자의 분포를 비교하였다. 또한, 환자군은 공격적 행동, 가족력, 아동기 발병 여부에 따라 두 군으로 나누어 비교하였다. DNA의 다형성 부의를 중합효소연쇄반응에 의해 증폭한 후 ,NlaⅢ제한효소법과 전기영동을 실시하여 유전자형을 판별하였다. 결 과 : 환자군 전체와 대조군 사이에 유전자형과 대립유전자 빈도의 차이가 없었다. 가족력이 있는 환자군을 대조군과 비교하였을 때는, 대립유전자 L을 포함하는 유전자형과 그렇지 않은 유전자형의 분포에 있어 유의한 차이를 보였다(p=0.02, OR=3.9, 95% Cl=1.1∼14.3). 결 론 : 한국인 정신분열병과 기능성 COMT 유전자 다형성 사이에 유의한 관련성이 없었다. 따라서, 기능성 COMT 유전자 다형성이 한국인 정신분열병의 발병에 영향을 주지 못한다. 그러나, 정신분열병 가족력과 기능성 COMT유전자 다형성의 관련성이 시시돠어, 향후 본 연구에서 나타난 방법론적인 문제점을 보완하고 추가 대상자를 확보한 연구가 필요하다. Objectives : Catechol-O-methyltransferase(COMT) is involved in the degradation of catecholamine meurotransmitters and has been investigated as a candidate gene in schizophrenia. Recently, possible relationship between functional COMT gene polymorphism and schizophrenia has been suggested. To address the possible role of functional COMT gene polymorphism in the predisposition to schizophrenia, we carried out an association study in Korean schizophrenic patients and controls. Methods : One hundred and three Korean inpatients diagnosed as schizophrenia and 103 age and sex matched controls were selected as study subjects. Patients were subgrouped into two groups on the basis of history of aggressive behavior, family history of schizophrenia and related disorders, and age at onset. We determined COMT genotypes using PCR of the relevant region followed by digestion with NlaⅢ and electrophoresis. Results : No significant differences of allele and genotype frequencies were noted between patients and controls. However, when patients were categorized by the presence of family history of schizophrenia and related disorders, patients with family history showed almost 4 fold higher frequency of having COMT L allele containing genotype compared to controls(p=0.02, OR=3.9, 9.5% CI=1.10-14.33). Conclusion : Although our results do not support an association between functional polymorphism of COMT gene and schizophrenia overall, the findings suggest an association between functional COMT gene polymorphism and familial schizophrenia. Further studies with large samples are needed to confirm this association.

Antiemetic and Myeloprotective Effects of <i> Rhus verniciflua Stoke</i> in a Cisplatin-Induced Rat Model

Kim, Hyo-Seon,Kim, Hyeong-Geug,Im, Hwi-Jin,Lee, Jin-Seok,Lee, Sung-Bae,Kim, Won-Yong,Lee, Hye-Won,Lee, Sam-Keun,Byun, Chang Kyu,Son, Chang-Gue Hindawi Publishing Corporation 2017 Evidence-based Complementary and Alternative Medic Vol.2017 No.-

<P><I>Rhus verniciflua</I> Stoke has been commonly used in traditional medicine to treat gastrointestinal (GI) dysfunction diseases. In order to investigate pharmacological properties of<I> Rhus verniciflua</I> Stoke water extract (RVX) on cisplatin-induced amnesia, RVX (0, 25, 50, or 100 mg/kg) was orally administrated for five consecutive days after a single intraperitoneal injection of cisplatin (6 mg/kg) to SD rat. Cisplatin injection significantly increased the kaolin intake (emesis) but reduced the normal diet intake (anorexia) whereas the RVX treatment significantly improved these abnormal diet behaviors at both the acute and delayed phase. The serotonin concentration and the related gene expressions (5-HT3 receptors and SERT) in small intestine tissue were abnormally altered by cisplatin injection, which were significantly attenuated by the RVX treatment. Histological findings of gastrointestinal tracts, as well as the proteins level of proinflammatory cytokines (TNF-<I>α</I>, IL-6, and IL-1<I>β</I>), revealed the beneficial effect of RVX on cisplatin-induced gastrointestinal inflammation. In addition, RVX significantly improved cisplatin-induced myelosuppression, as evidenced by the observation of leukopenia and by histological examinations in bone marrow. Our findings collectively indicated<I> Rhus verniciflua</I> Stoke improved the resistance of rats to chemotherapy-related adverse effects in the gastrointestinal track and bone marrow.</P>

Pharmacokinetics of SH003 which is a candidate for new anticancer agent in rat

Hyeong Jun Kim,In Sook Kim,Min Sun Choi,Hyun Kim,Young Seok Ji,So Young Yoon,Ki Beum Kim,In Yong Bae,Ko Sung Kyu,Hye Hyun Yoo 한국분석과학회 2016 학술대회논문집 Vol.2016 No.11

A Herbal Formula, CGXII, Exerts Antihepatofibrotic Effect in Dimethylnitrosamine-Induced SD Rat Model

Kim, Hyo-Seon,Kim, Hyeong-Geug,Lee, Hye-Won,Lee, Sung-Bae,Lee, Jin-Seok,Im, Hwi-Jin,Kim, Won-Yong,Lee, Dong-Soo,Son, Chang-Gue Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>We aimed to evaluate the antihepatofibrotic effects of CGXII, an aqueous extract which is composed of<I> A. iwayomogi</I>,<I> A. xanthioides</I>, and<I> S. miltiorrhiza</I>, against dimethylnitrosamine- (DMN-) induced hepatofibrosis. Male Sprague Dawley rats were intraperitoneally injected with 10 mg/kg of DMN for 4 weeks (three consecutive days weekly). Rats were orally given distilled water, CGXII (50 or 100 mg/kg), or dimethyl dimethoxy biphenyl dicarboxylate (50 mg/kg) daily. DMN injection caused substantial alteration of total body weight and liver and spleen mass, whereas they were notably normalized by CGXII. CGXII treatment also markedly attenuated the elevation of serum aspartate aminotransferase and alanine aminotransferase levels, hepatic lipid peroxidation, and protein carbonyl contents. Collagen accumulation in hepatic tissue evidenced by histopathological analysis and quantitative assessment of hepatic hydroxyproline was ameliorated by CGXII. Immunohistochemistry analysis revealed decreased <I>α</I>-smooth muscle actin supporting the antihepatofibrotic effect of CGXII. The profibrogenic cytokines transforming growth factor-<I>β</I>, platelet-derived growth factor-<I>β</I>, and connective tissue growth factor were increased by DMN injection. Administration of CGXII normalized the protein and gene expression levels of these cytokines. Our findings suggest that CGXII lowers the levels of profibrogenic cytokines and thereby exerts antifibrotic effects.</P>

Effects of β-Glucan from Aureobasidium pullulans on Acute Inflammation in Mice

Hyeong-Dong Kim,Bok-ryeon Park,Hee-Jeong Jang,Lin-Su Kim,Hyeung-Sik Lee,Sae-Kwang Ku,Hyung-Rae Cho,Seung-bae Moon,Hyun-Dong Shin,Kun-Ju Yang 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3

The effects of β-glucan isolated from Aureobasidium pullulans were observed on acute xyleneinduced inflammation. β-glucan at a dose of 62.5, 125 or 250 mg/kg were administered once orally to xylene-treated mice (0.03 mL of xylene was applied on the anterior surface of the right ear to induce inflammation), and the body weight change, ear weight, histological profiles and histomorphometrical analyses of ear were conducted upon sacrifice. The xylene was topically applied 30min after dosing with β-glucan. The results were compared to those of diclofenac, indomethacin and dexamethasone (15 mg/kg injected once intraperitoneally). All animals were sacrificed 2 h after xylene application. Xylene application resulted in marked increases in induced ear weights compared to that of intact control ear; hence, the differences between intact and induced ear were also significantly increased. The histological characteristics of acute inflammation, such as severe vasodilation, edematous changes of skin and infiltration of inflammatory cells, were detected in xylene-treated control ears with marked increase in the thickness of the ear tissues. However, these xylene-induced acute inflammatory changes were significantly and dose-dependently decreased by β-glucan treatment. We conclude that β-glucan from A. pullulans has a somewhat favorable effect in the reduction of the acute inflammatory responses induced by xylene application in mice.