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Lee, Nahum,Lee, Sang-Hyuk,Baek, Kiheon,Kim, Byung-Gee Springer International 2015 Applied microbiology and biotechnology Vol.99 No.19
<P>Recombinant tyrosinase from Streptomyces avermitilis MA4680, MelC2 (gi:499291317), was heterologously expressed in Escherichia coli BL21 (DE3). The expression level of active MelC2 was increased by the codon-optimized MelC1 caddie protein (KP198295.1). By performing saturation mutagenesis of the Y91 residue of MelC1, it was found that aromatic residues such as Y, F, and W at the 91st position help produce a correctly folded conformation of MelC2. The recombinant MelC2 was utilized as a biocatalyst to convert trans-resveratrol into piceatannol. In order to improve the product yield through suppression of the formation of melanin, a by-product, an increase in the ratio of monooxygenation (k (1)) to dioxygenation (k (2)) of MelC2 is desirable. This was achieved by a combination of protein engineering and regeneration of NADH with glucose dehydrogenase (GDH). Saturation mutagenesis was performed at 15 residues within 8- radius from copper ions of MelC2. A total of 2760 mutants were examined (99.7 % probability for NNK codon) and I41Y, a mutant, was screened. The ratio of k (1) to k (2) of the mutant increased sevenfold on tyrosine and fivefold on resveratrol, when compared to wild-type MelC2. As a result, the overall product yield from 500 mu M resveratrol in 50-mL reaction was 15.4 % (77.4 mu M piceatannol), 1.7 times higher than wild type. When I41Y was incorporated with the NADH regeneration system, the total product yield was 58.0 %, an eightfold increase (290.2 mu M of piceatannol).</P>
Implementation of a Videoconferencing System between Multiple Family Medicine Departments
Lee, Kee Hyuck,Kim, Ju Young,Lee, Kiheon,Cho, Belong,Yang, Jeong Hee,Goh, Eurah,Bae, Woo Kyung The Korean Academy of Family Medicine 2011 Korean Journal of Family Medicine Vol.32 No.5
<P>Attending conferences is important for doctors and residents in family medicine. Nevertheless, departments of family medicine at many hospitals find it difficult to hold regular conferences. Holding joint videoconferences between Family Medicine Departments of several hospitals through a videoconferencing system could solve this problem. Therefore, Family Medicine Departments of Seoul National University Hospital, Seoul National University Bundang Hospital, and Kangwon National University Hospital decided to hold regular joint videoconferences via a videoconferencing system. Eighty-one joint videoconferences were held from April 1 to October 29, 2010. PowerPoint slideshows were transferred to the other two locations in the same resolution as presenter's monitor. Image and voice of the speaker were transferred in real time and in acceptable quality. Joint videoconferences are feasible, satisfactory and useful for medical education, especially when individual family medicine departments are small and lack resources to hold face-to-face conferences. We expect that more family medicine departments will choose to participate in implementing similar joint videoconferencing systems in the future.</P>
( Dongjin Lee ),( Jisu Bae ),( Jungu Kang ),( Suyoung Lee ),( Kiheon Kim ) 한국폐기물자원순환학회(구 한국폐기물학회) 2015 한국폐기물자원순환학회 3RINCs초록집 Vol.2015 No.-
The purpose of this study was to compare the methane productivity depending on VS and CODcr in anaerobic digestion facilities treating food waste leachate. Among the operating bio-gasification facilities, efficient bio-gasification facilities were selected as target for this study. Reviewing application of Volatile solid (VS) or CODcr management standards is needed when measuring the methane production rate of food waste bio-gasification facilities. VS and CODcr of each sample from target facilities were analyzed based on the same value of nutritional ingredients (carbohydrate, protein, fat). Theoretical methane production based on VS and CODcr were 0.48 m<sup>3</sup> <sub>CH4</sub>/kgVS, 0.35 m<sup>3</sup> <sub>CH4</sub>/kg<sub>CODcr</sub> respectively. Considering the average degradation rates of anaerobic digestion processes (VS 74.6 %, CODcr 77.4 %), Methane production based on VS and CODcr were 0.48 m<sup>3</sup> <sub>CH4</sub>/kgVS, 0.34 m<sup>3</sup> <sub>CH4</sub>/kg<sub>CODcr</sub> which is similar to theoretical value. The results showed that methane production rates of VS and CODcr were feasible in both measurement items. However, considering the field applicability and convenience of analysis, It is desirable to set the methane productivity based on VS as the reference value.
Na Hyun Kim,Jong Soo Han,Woo Kyung Bae,Joo Young Kim,Kiheon Lee,이혜진,Kee Hyuck Lee,Se Young Jung,HanKyung Lee,Hee-Yeong Jeong,Young A Kim,Suyoun Sim,Ji-Won Kang 대한가정의학회 2022 Korean Journal of Family Medicine Vol.43 No.4
Background: Since the era of “thyroid cancer epidemic,” many Korean academic societies discouraged the use of ultrasonography in healthy individuals and revised the Korean Thyroid Imaging Reporting and Data System to ad-dress the overscreening and overdiagnosis issues. This study aimed to evaluate the change in the diagnostic effec-tiveness of thyroid cancer screening over the last decade.Methods: This single-center, retrospective observational study analyzed the data of 125,962 thyroid nodules ob-tained during cancer screening at the health promotion center of Seoul National University Bundang Hospital from 2010 to 2019. Only 327 thyroid cancer cases pathologically confirmed by fine-needle aspiration (FNA) were includ-ed in the study. The strength of the association between the number of FNA and (1) the number of thyroid cancer diagnoses, (2) the positive predictive values (PPVs), and (3) the difference in PPV from the previous year were eval-uated using Pearson’s correlation analysis.Results: The number of thyroid FNA biopsies as well as the thyroid cancer diagnoses decreased from 2010 to 2019 (166 to 48 [-71.1%] vs. 43 to 22 [-48.8%]). The PPV of FNA biopsies increased from 25.9% to 45.8% (+76.8%) and was negatively correlated with the number of FNA biopsies performed (R=-0.87, P<0.001). The difference in PPV from the previous year increased similarly but without statistical significance (R=-0.59, P=0.09).Conclusion: The diagnostic efficiency of thyroid cancer screening has increased over the last decade, as evidenced by the increasing PPV of FNA biopsies.
Fruit Intake and Changes of Cardio- Metabolic Risk Factors in People with Obesity
Shiao Shih Liu,Ju Young Kim,박정하,SOHYE KIM,Kiheon Lee,Woo Kyung Bae,Kee Hyuck Lee,Jong-Soo Han,Hye Jin Lee,Se Young Jung 대한가정의학회 2021 Korean Journal of Family Medicine Vol.42 No.5
Background: This study aimed to explore the relationship between fruit intake, changes in fruit intake, and changes in cardiometabolic factors in people with obesity. Methods: A total of 21,270 subjects (8,718 men, 12,552 women) aged 40 years and over, from the Korean-based Genome and Epidemiology Study, were followed up for an average of 4.4 years. Fruit intake was assessed using a food frequency questionnaire at baseline and the second follow-up. The beta coefficient and confidence intervals for changes in cardiometabolic risk factors according to fruit consumption were calculated using a linear regression model. Results: In men, the abdominal circumference decreased with changes in fruit intake (P=0.029). Fruit intake and increased fruit intake in men were associated with a lower systolic blood pressure (P=0.012 and P=0.02, respectively) and lower triglyceride levels (P=0.002 and P<0.001, respectively). In women, abdominal circumference decreased with both fruit intake and increased fruit intake (P<0.001 and P=0.013, respectively). Systolic blood pressure and triglycerides tended to decrease only with fruit intake (P=0.048 and P<0.001, respectively). Unlike in men, fasting blood glucose tended to decrease in women with both fruit intake and increased fruit intake (P=0.011 and P=0.005, respectively). Conclusion: Fruit intake and increased fruit intake may have beneficial effects on cardiometabolic risk factors among individuals who are obese.
Risk Factors of Cardiovascular Disease according to Alcohol Behavioral Change after Cancer Diagnosis
Eun Mi Bae,In Young Cho,Ji-Hye Jun,Kiheon Lee,Ju Young Kim,Woo Kyung Bae,이혜진,Jong-Soo Han,Se Young Jung,Kee Hyuck Lee,Sarah Kim,Hye Yeon Koo,Sang Jin Cho,Houbuem Lee,Chuelmin Paek 대한가정의학회 2020 Korean Journal of Family Medicine Vol.41 No.4
Background: Problem drinking increases the incidence of all-cause mortality and specific cancers, and persistent drinking is associated with cardiovascular disease in certain cancer survivors. This study analyzed the cardiovascular risk factors before and after diagnosis in Korean cancer survivors. Methods: Data for the period between 2002 and 2013 were collected from the National Health Insurance Service Health-Examinee Cohort Database. Among the 27,835 patients included, those with moderate alcohol consumption before and after cancer diagnosis were excluded. Problem drinking was defined as males under 65 years consuming over 14 glasses a week, and males over 65 years or females consuming over seven glasses a week. A t-test, chi-square test, and linear regression analysis were performed for differences in cardiovascular risk factors and differences according to cancer types. Results: There was a difference in the body mass index, systolic and diastolic blood pressure, and total cholesterol among patients who became moderate drinkers after diagnosis, but fasting blood glucose did not show any significant changes. Risk factors for cardiovascular disease were analyzed in patients with liver, stomach, rectal, and breast cancer with improved drinking behavior, and there were significant differences in body mass index, systolic and diastolic blood pressure, fasting blood glucose, and total cholesterol in stomach cancer patients. Conclusion: Moderate drinking can lower cardiovascular risk in cancer survivors, and among the many drinkingrelated cancers, stomach cancer patients demonstrated significantly reduced cardiovascular risk factors.