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간내 담석과 동반된 간내 담도암에서의 p53과 Ki-ras의 발현
김명환,이성구,서동완,이승규,남승우,박능화,민영일,김연석,심기남,공경엽 대한소화기학회 1997 대한소화기학회지 Vol.30 No.5
Background/Aims: Hepatolithiasis or primary intrahepatic stones may be accompanied later by intrahepatic cholangiocarcinoma. This cancerous lesion is frequently associated with atypical hyperplastic epithelium that was suspected of being precancerous. To investigate the Ki-ras or p53 mutation may play a role in carcinogenesis, and to determine whether atypical hyperplastic epithelium may be precancerous, this study was performed in intrahepatic cholangiocarcinomas associated with hepatolithiasis. Methods: We examined 12 patients with intrahepatic cholangiocarcinomas associated with hepatolithiasis. Ki-ras point mutations were assessed by direct DNA sequencing. Expressions of p53 protein were immunohistochemically assessed. The overexpression of p53 and point mutation of Ki-ras were examined in normal, hyperplastic, atypical hyperplastic, and can- cerous tissues separately. Results: The expressions of p53 protein were detected in 4 (33%) of 12 cholangiocarcinomas and Ki-ras point mutations at codon 12 were found in 2 (17%) of 12 cholangiocarcinomas. In those two carcinoma cases which contained the mutant sequence of Ki-ras, the same point mutation from wild type GGT (glycine) to GAT (aspartic acid) was. found in the associated atypical hyperplastic epithelium. However, none of the normal and hyperplastic epithelium harbored Ki-ras or p53 mutations. Conclusions: The overexpression of p53 may play a part in the carcinogenesis of some intrahepatic cholangiocarcinomas associated with hepatolithiasis, whereas the role of Ki-ras mutations in those cases is dubious. However, Ki-ras point mutation at codon 12 may be responsible for either cancer or atypical hyperplastic epithelium associated with hepatolithiasis in certain cases, suggesting atypical hyperplastic epithelium may give rise to carcinoma.
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수족냉증(手足冷症) 환자(患者)에 대(對)한 컴퓨터 적외선(赤外線) 체열촬영(體熱撮影)의 의의(意義)
조유경,오수완,조남희,김동묵,김진성,류봉하,박동원,류기원,Cho, Yu-Kyung,Oh, Su-Wan,Cho, Nam-Hee,Kim, Dong-Mook,Kim, Jin-Seong,Ryu, Bong-Ha,Park, Dong-Won,Ryu, Ki-Won 대한한방내과학회 1998 大韓韓方內科學會誌 Vol.19 No.2
To make a objective diagnosis of the syndrome of cold-limbs, We investigated the 20 patients with cold limbs and GI trouble and 20 normal people as a control group. And we compared the thermal difference between Chon-jung(CV17) and Chung-wan(CV12 中脘), Chon-jung(CV17) and Ki-hae(氣海 CV6) and we compared the thermal differences of No-gung(PE8 勞宮) and Yong-chon(湧泉 KI1), too. The results were as follows. 1. All 20 patients had GI trouble and cold limbs. They had the symptom-Indigestion(16 cases-80%) with heart burn, tympanites, abdominal distention, hiccup, belching. Beside that symptom they also had constipation(6 cases-30%), diarrhea(3 cases-15%), headache & dizziness(6 cases-30%). And some had the menstrual syndrome, chronic fatigue, palpitation, insomnia, edema, arthralgia. 2. The thermal difference of the palms between the patients group and the control group were $25.70^{\circ}C,\;25.82^{\circ}C$, but they were not significant. 3. The thermal difference of the soles between the patients group and the control group were $23.58^{\circ}C,\;24.42^{\circ}C$ and the significancy was P=0.020 so it was significant(P<0.05). 4. The thermal difference of the palms and Chon-jung(CV17) between the patients group and the control group were $1.08^{\circ}C,\;0.76^{\circ}C$, but they were not significant. 5. The thermal difference of the sales and Chon-jung(CV17) between the patients group and the control group were $3.01^{\circ}C,\;1.90^{\circ}C$ and the significancy was P=0.003 so it was significant(P<0.05). 6. The thermal difference of Chon-jung(CV17) and Chung-wan(CV12 中脘) between the patients group and the control group was $0.30^{\circ}C,\;0.62^{\circ}C$ and the significancy was P=0.793 so it was significant(P<0.05). 7. The thermal difference of Chon-jung(CV17) and Ki-hae(CV6 氣海) between the patients group and the control group was $0.53^{\circ}C,\;0.68^{\circ}C$, but they were not significant. From the above, I could find the significance of D.I.T.I to diagnose the cold-feet not the cold-hands and the thermal difference between the trunk and limbs was more significant on cold-feet than on cold-hand, either.
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서버기반의 상호대화방식 객체 모델을 이용한 대화형 웹사이트 구현
고완기,문석환 제주한라대학 1997 論文集 Vol.21 No.-
The number of users who want to get new information from the internet which called "Ocean of Information" are increasing every year. For Company, they connect to outside network system which called internet with inside network system for improving their competition against others and re - thinking their own image through an interchange of information freely. Thus, it is very important that we have to protect of information so that we can make an information - oriented society. We should build closed web site for the protection which is known to hackers who can exchange their own information and to public users who can get any information through any source from Unix and TCP/IP. It can be possible to protect site without firewall. In regard to certification of the users and maintain, we can explain ASP (Action Service Page) which is easily able to manage with Cookies Collection and can build closed web site model with ASP. This thesis introduces new web site by ASP for person to person among closed web users or each web user to server. That is, this is the way to build perfect protection web site for closed users. This method will develope web shopping system by internet or controlled conversation system which must be protected for the same company groups.
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이기완,고태석 동신대학교 환경연구소 1998 환경연구 Vol.3 No.1
황룡강을 수원으로 하는 상수원수와 침전상징수의 정수를 위하여 MF(정밀여과)공정을 적용하였다. 현장에서의 실험결과는 낮은 운전압력일수록 높은 탁도 제거율을 보였으며, 상징수의 탁도는 2~4 NTU로서 MF처리시 1kg/㎠에서는 0.3~0.4 NTU로 2kg/㎠에서는 0.4~0.5 NTU로 감소되었다. 이들 유출탁도는 음용수 기준인 1.1 NTU를 민족 할 정도이었다. 원수는 15~20 NTU의 높은 탁도로서 원수를 직접 MF처리할 경우, 유출수는 1kg/㎠에서 1.9~4.8 NTU로 2kg/㎠ 에서 2.1~4.9 NTU로 나타났다. 이들 탁도는 처리수 기준을 초과하였으므로, 기준을 만족하기 위해서는 다단계의 MF 처리가 권장된다. 막 의 역세척 소요시간은 약 6분이 적합하였다. MF처리공정은 재래식 모래여과기와 비교할 때 작은 소요부지 면적, 막의 내구성과 수명, 약품비등에서 수처리에 효과적임을 보여주었다. MF(Micro Filtration) process was applied to the treatment of raw water and clarified water in water treatment plant sourced from the Hwangryong river. As results of experiments and field study the lower the operating pressure, the higher the turbidity removed. Turbidity of clarified water were reduced from 2~4 to 0.3~0.4 NTU at 1kg/㎠ and 0.4~0.5 NTU at 2kg/㎠ by MF process. These effluent turbidities were acceptable to the drinking water standard of 1.1 NTU. For the raw water of high turbidity with 15~20 NTU the effluents were 1.9~4.8 NTU at 1kg/㎠ and 2.1~4.9 NTU at 2kg/㎠. These turbidities exceeded the standard value of treated water. To meet the standard, multistage operations were executed with the raw water and its turbidity decreased to 0.1 NTU. The proper time for backwashing of the unit was about 6 minutes. Compared with a traditional sand filter, MF process showed the effectiveness of Water treatment due to small area, long life spans and less chericals required.
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裵基完 단국대학교 1979 論文集 Vol.13 No.-
In analysing rural economy as well as rural society, requires a study of farmers mental structure, based on which the modernization of rural life shall be sought for. Agriculture, being as a important sector of the economy, not only provides basic foodgrains and industrical materials but also contributes especially in Korea, greatly for the economic growth as well as social stability. But, agriculture is by nature, to decline as the economy grows and therefor, the income difference of the sector with other sectors is to expand. The course is being such, it is necessary, first, to raise agricultural productivity with expansion of landholdings, cooperative farmming, extension or remedy of production base, mechan ization and application of higher technology; senond, to improve the structure of agricultural production. In seeking such objectives farmers are obliged to study whether or not the structural improvement of rural economy and society are required of and in which manner the improvement is to be reached. Besides it is an absolute factor for that end to make integrated and cooperative efforts.
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Chlorobenzilate가 Mouse 간세포의 미세구조에 미치는 영향
安基亮,김완식,鄭鎬三,李圭植 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.2
Chlorobenzilate which is the organophosphorus acaricide, is very toxic to man and livestock. Chlrobenzilate is accumulated in the fat tissue like other organophosphorus insecticide so that it is extremely toxic. Its toxicity on the central nervous system produce extreme convulsion but its mechanism has not been understood. And also the degenerative changes in the liver, kidney and brain are appeared by its chronic toxicity. Chlorobenzilate is hydrolysed in the liver that its metabolites ay damage the hepatic parenchymal cells. So the authors have undertaken to pursue the toxic effect of the chlorobenzilate on the organelles of the liver cells. Albino mice were used as experimental animals. The experimental animals sacrificed at 6 and 24 hours after administration of 641 mg (0.5ml) of chlorobenzilate per Kilogram of body weight. The specimens obtained from the liver were fixed in 2% glutaraldehyde-2.5% paraformaldehyde phosphate buffer (pH 7.2) and sliced with 600-800 A° thickness. The specimens were stained with uranyl acetate and lead nitrate and then observed with the electron microscope. The results were as follows. 1. Proliferation of smooth endoplasmic reticulum and depletion of glycogen particles were observed. 2. An increase in the number of secondary lysosomes, multivesicular bodies, and lysosomes were observed. 3. The atropy of the cisternae of the Golgi complex was observed. The disruption of the cisternae of rough endoplasmic reticulum was associated with detachment of the membrane bound ribosomes.
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