콩아이스크림의 상품화를 위한 고정화 효소의 이용

이숙영,이현옥,노숙령,김기숙,이복희,박미정 중앙대학교 생활과학연구소 2002 생활과학논집 Vol.15 No.-

The study was to investigate the functional properties of soy protein isolate hydrolyzed by immobilized α-chymotrypsin and the quality characteristics of soy ice cream made by the soy protein isolate treated with immobilized α-chymotrypsin. Up to the hydrolysis rate of 22.9%, treatement by immobilized α-chymotrypsin costed more time than that of α-chymotrypsin. Foam and emulsion capacity were decreased by the number of repetitive usage. Apparent viscosity and overrun of soy ice cream treated with immobilized α-chymotrypsin and α-chymotrypsin were shown similar tendency. In the sensory characteristics, soy ice cream by enzyme treatment was evaluated as much acceptable than untreacted soy ice cream in the item of beany flavor, icy feel and overall quality. For efficient use of immobilized α-chymotrypsin industrially, α-chymotrypsin immobilized on glass beads can be reused for about 11 times more than α-chymotrypsin.

대학생들의 떡에 대한 기호도 및 이용현황

이현옥,노숙령,김기숙,이숙영,이복희,박미정 중앙대학교 생활과학연구소 2002 생활과학논집 Vol.15 No.-

The survey study was conducted to investigate the rice cake consumption pattern including the frequency of intake and the degree of preference and the area of improvement for Korean rice cake among college students. About the motive for purchases of rice cake, 'taste is good(43.3%)' was the highest, and 'eat as a snack' the next (42.9%). The reason which does not eat the rice cake frequently was uncomfortable to eat (24.1%), insipid(21.4%), not variously assorted(17.9%) in the order named. The college students surveyed were eating rice cake as a snack mainly. They wanted the rice cake to be charmed and be in pretty shape, and fusion style for gift. And they wanted to be rice cake more sweetened and cool taste like ice cream. Therefore, traditional rice cake is required for further research for the development of product which is more acceptable for the young adults.

관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

Substance P restores normal skin architecture and reduces epidermal infiltration of sensory nerve fiber in TNCB-induced atopic dermatitis-like lesions in NC/Nga mice

Choi, Hyeongwon,Kim, Dong-jin,Nam, Seungwoo,Lim, Sunki,Hwang, Jae-Sung,Park, Ki Sook,Hong, Hyun Sook,Won, Younsun,Shin, Min Kyung,Chung, Eunkyung,Son, Youngsook Elsevier 2018 JOURNAL OF DERMATOLOGICAL SCIENCE Vol.89 No.3

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Atopic dermatitis (AD) is a chronic inflammatory skin disorder characterized by intense pruritus and eczematous lesion. Substance P (SP) is an 11-amino-acid endogenous neuropeptide that belongs to the tachykinin family and several reports recently have supported the anti-inflammatory and tissue repairing roles of SP.</P> <P><B>Objective</B></P> <P>In this study, we investigated whether SP can improve AD symptoms, especially the impaired skin barrier function, in 2, 4, 6-trinitrochlorobenzene (TNCB)-induced chronic dermatitis of NC/Nga mice or not.</P> <P><B>Method</B></P> <P>AD-like dermatitis was induced in NC/Nga mice by repeated sensitization with TNCB for 5 weeks. The experimental group designations and topical treatments were as follows: vehicle group (AD-VE); SP group (AD-SP); and SP with NK1R antagonist CP99994 (AD-SP-A) group. Histological analysis was performed to evaluate epidermal differentiation, dermal integrity, and epidermal nerve innervation in AD-like lesions. The skin barrier functions and pruritus of NC/Nga mice were evaluated by measuring transepidermal water loss (TEWL) and scratching behavior, respectively.</P> <P><B>Result</B></P> <P>Topical SP treatment resulted in significant down-regulation of Ki67 and the abnormal-type keratins (K) K6, K16, and K17, restoration of filaggrin and claudin-1, marked reduction of TEWL, and restoration of basement membrane and dermal collagen deposition, even under continuous sensitization of low dose TNCB. In addition, SP significantly reduced innervation of itch-evoking nerve fibers, gelatinase activity and nerve growth factor (NGF) expression in the epidermis but upregulated semaphorin-3A (Sema3A) expression in the epidermis, along with reduced scratching behavior in TNCB-treated NC/Nga mice. All of these effects were completely reversed by co-treatment with the NK1R antagonist CP99994. In cultured human keratinocytes, SP treatment reduced expression of TGF-α, but upregulated TGF-β and Sema3A.</P> <P><B>Conclusion</B></P> <P>Topically administered SP can restore normal skin barrier function, reduce epidermal infiltration of itch-evoking nerve fibers in the AD-like skin lesions, and alleviate scratching behavior. Thus, SP may be proposed as a potential medication for chronic dermatitis and AD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Topical Substance P treatment reduced AD-like symptoms in TNCB-induced NC/Nga mice. </LI> <LI> Topical Substance P treatment reduced epidermal acanthosis and expressed normal keratin profiles in AD-like mice. </LI> <LI> Topical Substance P treatment regulated both small nerve attraction and repulsion factors in AD-like skin. </LI> <LI> Topical Substance P treatment recovered skin barrier function and reduced pruritus in AD-like mice. </LI> </UL> </P>

산성화가 식물플랑크톤 종조성에 미치는 영향

채기숙,김범철 江原大學校 附設 環境硏究所 1997 環境硏究 Vol.14 No.-

The effects of acidification on phytoplankton composition were observed by enclosure experiments. The surface water of Lake Soyang was acidified to 5.5, 5.0, or 4.5 with H₂SO₄, and the change of species composition and biomass of phytoplankton were observed. Axenic cultures of two algae, a green alga(Scenedesmus obliquus) and a cyano bacterium(Microcystis aeruginosa) was also used to test the effects of acidification. Dominant phytoplankton in Lake Soyang were Asterionella in winter, and peridinium and Anabaena in summer. In acidified condition, dominant species changed from cyanobacteria to green algae. At pH 4.5, most algal cells showed morphological damage. Species richness decreased with decreasing pH. The growth rate of Anabaena declined at below pH5.5. Whereas, the growth rate of Peridinium, Fragilaria, and Asterionella decreased only at pH 4.5. Green alga, Desmidium and Dictyosphaerium, were Anabaena, green algal increased even at pH 4.5 and grew slower at neutral pH. In axenic cultures, the growth of Microcystis aeruginosa was inhibited at 4.0 and 5.0 and showed maximum at pH 9.0. Scenedesmus obliquus also grew well at pH 5.0 and showed maximum at 9.0 At pH 4.0, Scenedesmus did not grow at all.

Vancomycin내성 Enterococci의 빈도 조사 및 중합효소연쇄반응을 이용한 유전자형의 분석

홍기숙,강은숙,이미애 대한임상병리학회 1998 대한임상병리학회지 Vol.18 No.3

南北韓 統一條約에 관한 硏究 : 統一條約締結能力과 締結節次를 中心으로 Unification Treaty-making Capacity and Treaty-making Procedure

金永基,金明基,金淑子,劉夏榮 명지대학교 대학원 1998 대학원논문집 Vol.2 No.-

Whether Korean peninsular's dividing cause from the dividing of 38 degree line according to the "General order No 1" in 1945 or from the "Armistice Agreement" in 1953, that was not our will but outside's power. We can not say that reunification of our country would depend on foreign power because its dividing caused by outside power. Enter-Korea have been hostilize each other regarding as irregular group under dividing seatus. It is certain that our facing problem and ultimate objective is reunification not the naturalization of dividing. Accordingly, we have to consider sincerely reunification of our country considering enough inner or outer conditions given to us. Not only South Korea but North have a ability to take a reunification treaty with domestic law and international. The problem of future is when and how we will have to pursuit discussion first procedure. Our country require earnestly our wisidom and wit than ever for reunification. The responsible offiser of reunification policy have to decide sincerely reunification policy according to the high opinion of the export and many people working every parts.

균형직교 중심합성계획법

정란숙,김기호 중앙대학교 통계연구소 1998 統計論文集 Vol.- No.5

중심합성계획은 반응표면방법론에서 일반적으로 사용하는 반응표면계획법이다. 이때 중심합성계획이 같은 블록크기와 직교블록을 만족하도록 적절한 축점의 값 α을 찾아준다. 본 연구에서는 실험의 편리함과 분석의 효율성을 고려하여 이러한 균형직교 중심합성계획법을 연구하였다.

편도체 기저외측핵의 AP5 투여가 상승된 경악반응의 습득에 미치는 영향

서미숙,조소현,김기석,이만영 한국심리학회 한국심리학회지 생물 및 생리 Vol.5 No.1

본 연구는 고전적 공포조건화에 관여하는 장소로 알려진 편도체의 기저 외측핵에 장기상승작용(Long-Term Potentiation: LTP)의 생성을 차단하는 약물인 MNDA (N-methyl-D-aspartate)길항제 AP5를 미세주입함으로써 시각적 조건자극(CS)을 사용한 경우에, 상승된 경악 반응의 습득이 차단되는지를 보고자 하였다. 동물은 AP5-AP5, AP5-식염수, 식염수-AP5, 식염수-식염수 집단으로 나누어서 CS-US 배쌍직전에 AP5나 식염수를 주입한 후 훈련시켰다고 다시 검사직전에 AP5 혹은 식염수를 주입하였다. AP5-AP5집단과 AP5-식염수집단은 상승된 경악반응을 보이지 않았고, 식염수-AP5, 식염수-식염수 집단은 상승된 경악반응을 보였다. CS-US배쌍 직전에 주입한 AP5는 상승된 경악반응을 차단하였고, 검사직전에 주입한 AP5는 상승된 경악반응을 차단하지 않은 결과는 AP5가 습득을 차단했으되 그 표현에는 영향을 미치지 않았다고 해석된다. 그리고 AP5-AP5, AP5-식염수 집단이 상승률에서 유의미한 차이를 보이지 않았으므로, 상승된 경악반응의 차단이 상태의존적 인출 실패에 기인한 것이 아니고, AP5 자체의 약효때문임을 알 수 있다. It has been known that the amygadala is the neural substrate for conditioned fear as well as unconditioned fear. Among the substructures of amygdala, the basolateral nucleus where CS and US inputs converge and LTP occurs, contains high densities of NMDA receptors and so seems to have a critical role in synaptiplasticity. NMDA antagonist, AP5 prevent induction of Long-term potentiation, but not expression of LTP. LTP is an activity dependent enhancement of synaptic efficacy and is regarded the psysiological mechanisms that might underlie learning and memory. So this experiment was done to investigate what effect AP5 injection to the basolateral amygdala on the acquisition and expression of the fear conditioning, using the fear potentiated Startle paradigm. Animals were allocated to AP5-AP5, AP5-saline, saline-AP5, saline-saline groups. AP5 or saline was injected just before conditioning and testing. The re sult is that AP5-AP5, AP5-saline group didn't show the potentiated startle, comparative to the saline-AP5, saline-saline group, and AP5-AP5 group is not significantly different from AP5-saline. So we conclude that AP5 blocked the acquisition but not expression of conditio-ned fear-potentiated startle and convince that the blocking is not due to state-dependent retrieval failure.

광조사 방식이 복합레진 수복물의 변연누출에 미치는 영향

박은숙,김기옥,김성교 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.4

저광도 중합법, 펄스 지연 중합법, 초고광도 중합법 등의 광조사 방식이 광중합형 복합레진 수복물에서 의 중합수축응력에 미치는 영향을 알아보기 위해 in vitro에서 미네 누출 실험을 하였다. 80개의 발거된 소구치의 협측면에 5급 와동을 형성하고, 600mW/㎠로 30초간 광조사하는 보통 광도 중합군 300mW/㎠로 60초간 광조사하는 저광도 중합군, 400mW/㎠로 2초간 광조사하고 5분간 기다린 후 800mW/㎠로 10초간 최종 중합시키는 펄스-지연 중합군, 그리고 1930mW/㎠의 광도로 3초간 광조사하는 초고광도중합군 등의 4개의 군으로 나누어 hybrid형의 광중합 복합레진을 충전하고 각 군의 방법대로 중합한 후, 변연의 0.5mm 외부에 nail varnish를 도포하고 37℃, 2% metylene blue용액에 24시간 침적시켰다. 시편을 아크릴릭 레진에 매몰한 후 수복물의 중앙에서 종절단하여 입체현미경하에서 그 단면을 관찰한 후 법랑질과 상아질 변연으로 나누어 색소의 침투도를 측정하여 다음과 같은 결과를 얻었다. 1. 펄스 지연 중합군의 색소 침투도는 법랑질과 상아질 변연 모두에서 보통광도, 저광도 및 초고광도 중합군과 유의한 차이를 보이지 않았다(p>0.05) 2. 상아질 변연에서 초고광도 중합군은 보통광도 중합군과 저광도 중합군에 비해 유의하게 높은 색소 침투도를 보였다(p<0.05) 3. 법랑질 변연에서의 4개 군의 색소 침투도는 서로 유의한 차이를 보이지 않았다(p>0.05) 4. 모든 군에서 상아질 변연에서의 색소 침투도는 법랑질군에서의 색소 침투도에 비해 유의하게 놓았다.(p<0.05)