Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

시간-주파수 해석법을 이용한 파이프 내 유도초음파 모드의 식별에 관한 연구

박익근,김용권,조윤호,안연식,조용상 한국비파괴검사학회 2004 학술대회 논문집 Vol.- No.-

발전설비의 보수검사에 적용하기 위한 예비 연구로 comb transducer를 이용한 파이프 내에서의 유도 초음파 모드의 거동을 실험적으로 검증하였다. 유도초음파의 모드식별은 STFT 와 WT에 의한 시간-주파수해석을 통하여 최적의 모드를 선정하였다. 시간-주파수해석과 이론적 해석 방법인 분산 곡선을 비교한 결과 잘 일치함을 알 수 있었으며, pitch-catch법과 선단부로부터 반사된 신호를 모드 분석한 결과 L(0,1) 모드가 다른 모드에 비해 모드변환에 의한 영향이 적었다. 따라서 L(0,1)을 최적의 모드로 선정하고, 결함위치를 추정한 결과 유용함을 알 수 있었다.

Mitomycin-C 적용시간에 따른 누낭비강연결술의 수술결과

박근성,임진호,민병무,최시환,김창식,김용백 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

To determine the usefulness of application of Mitomycin-C (MMC) and effectiveness of different concentration of MMC in external DCR. A total of 100 eyes of 87 patients diagnosed with primary acquired nasolacrimal duct obstruction were assigned randomly to either a MMC group or control group. The surgical procedures in both groups were same. In MMC group, a piece of neurosurgical cottonoid soaked with 0.4 mg/ml MMC was applied to the osteotomy site and then was removed after 5 and 10 minutes differently. Nasoendoscopic examination was performed at 1 month, 3 months, and 6 months after surgery for all patients. The causes of epiphora as trauma (20 cases), failed DCR (1 case), sinus surgery (2 cases), unknown (77 cases). The overall anatomical success rate of the procedure was 88% and functional success rate was 79%. Obstruction site was related to anatomical surgical results. Anatomical surgical outcome at lower lacrimal sac level group of patients showed a statistically significant success rate, but functional surgical outcome showed no significant difference between the two groups. The anatomical success rate was 87.7% and the functional success rate was 84.2% in MMC group. The anatomical success rate was 88.3% and the functional success rate was 74.4% in control group. Application of MMC and it's application time were not related to surgical results.

Comb Transducer를 이용한 파이프 내 유도초음파 모드의 거동에 관한 연구

박익근,김용권,조윤호,안연식,조용상 한국비파괴검사학회 2004 한국비파괴검사학회지 Vol.24 No.2

발전설비의 보수검사에 적용하기 위한 예비 연구로 comb transducer를 이용한 파이프 내에서의 유도초음파 모드의 거동을 실험적으로 검증하였다. 유도초음파의 모드식별은 STFT와 WT에 의한 시간-주파수해석을 통하여 최적의 모드를 선정하였다. 시간-주파수해석과 이론적 해석 방법인 분산 곡선을 비교한 결과 잘 일치함을 알 수 있었으며, pitch-catch법과 선단부로부터 반사된 신호를 모두 분석한 결과 L(0,1) 모두가 다른 모드에 비해 모드변화에 의한 영향이 적었다. 따라서 L(0,1)을 최적의 모드로 선정하고, 결함위치를 추정한 결과 유용함을 알 수 있었다. A preliminary study of the behavior of ultrasonic guided wave mode in a pipe using a comb transducer for maintenance inspection of power plant facilities has been verified experimentally. The mode identification has been carried out in a pipe using the time-frequency analysis methods such as the wavelet transform(WT) and the short time Fourier transform (STFT), compared with theoretically calculated group velocity dispersion curves for longitudinal and flexural modes. The results are in good agreement with analytical predictions and show the effectiveness of using the time-frequency analysis method to identify the individual modes. It was found out that the longitudinal mode(0,l) is less affected by mode conversion compared with the other modes. Therefore, L(0,l) is selected as an optimal mode for the evaluation of the surface defect in a pipe.

불소가 함유된 교정용 레진의 접착강도 및 항우식 효과에 관한 연구

박근철,김용기 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.1

The frequent occurrence of enamel of decalcification around orthodontic bands or brackets still continues to be a problem for the clinicians. One of the numerous procedures suggested to overcome those probledms would be the use of fluoride-containing orthodontic resin. The purpose of this study was to compare the anticariogenic potential nad the shear bond strength of two orthodontic resins: GuoupⅠresin containing fluoride and GroupⅡresin with no fluoride.Histological observations were performed through polarizing light microscope and bond strength mesaurements was done by SEM. GroupⅡgenerally showed superior anticariogenic effect to GroupⅠ,positively demonstrating the beneficial effect of fluoride in lowering caries susceptibility. Both groups showed significantly decreased shear bond strength in 4-day specimen when compared to 1-day specimen. However,no significant defferences between groups could be found in terms of the forces required for debonding. In estimation of bonding area that failed,groupⅠpredominantly showed the pattern of adhesive failure whereas enamel detachment pattern in groupⅡ. The results of the present study strongly suggest the successful use of fluoride-containing othodontic resin in a sense of efforts to protect enamel from possible demineralization.

소아환자의 Porcelain Laminate Veneer를 이용한 임상증례보고

박근철,김용기 大韓小兒齒科學會 1991 大韓小兒齒科學會誌 Vol.18 No.2

A large portions of child population suffer esthetic problem during their course of growth caused by various dental defects especially on anterior region. Common crown defects are fracture,discoloration,malformation,abnormal spacing and etc. These anterior defects have a great importance on the normal psychological and social development of children especially in those who are starting their school life. Among various kinds of treatment approaches, porcelain laminate veneer restorations are now being widely used for the younger age group as well as adult population. A 11 year and 1 month old female patient with a chief complaint of anterior spacing caused by peg lateralis came to the pediatric dept. of Dankook University Dental hospital for treatment. Localized gingivectomy on laterals were performed to increase crown length and composite resin contouring of centrals of disto-incisal margin were done to restore the natural contours. Porcelain laminate veneers were fabricated indirectly in the laboratory and adapted on upper peg lateralis by usual manner. The treatment made her smile more acceptable and thus positive expectation on her development of normal character could be made.

경추손상이 의심되는 환자에서 경추전방 연부조직 폭 측정의 의의

박주경,김성근,윤한덕,허탁,조석주,민용일 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

Prevertebral soft tissue swelling of cervical spine lateral radiogram is well known as an indirect evidence of occult cervical spine injury. But the clinical and statistical value of it has been of debate. We tried to analyse the value of cervical prevertebral soft tissue width as a screening test for cervical spine injury by comparison of two categorized study groups; the control group, traumatized patients without cervical spine injury and the injury and the injury group, traumatized patients with bony cervical injury. The injury group consisted of patients admitted between September 1993 and December 1994 with an ED diagnosis of bony cervical injury. The control group consisted of patients admitted between January 1994 and June 1994 who received cervical spine lateral radiograph because of suspicion of cervical spine injury or as a routine check. In both group, we sampled the patients who received cervical spine lateral rediograph within 24 hours after injury and excluded the patients less than 15 years old and more than 65 years old, In the control group, we confirmed the patients had no problem in cervical spine of follow-up. 101 control patients and 68 injury patients were identified. The injury group was further divided into two subgroups: those with injuries at C1-C3 to the upper injury subgroup and those with injuries at C4-C7 to the lower injury sub group. The prevertebral soft tissue widths of injury group were larger than of control group at C2(mean 6.1mm versus 4.5mm) and also at C6(mean 13.4mm versus 11.2mm). Especially upper injury subgroup had quite large value of C2 prevertebral soft tissue width(mean 14.1mm). Nevertheless, both in C2 and C6, there is no cutoff value with acceptable sensitivity and specificity as a screening test. But the 7mm at C2 and 14mm at C6 had high specificity(90%) and were thought to be a good radiologic indicators of further evaluation.

韓國人 學生의 腦機能 優勢(Cerebral Dominance)와 손잡이(Handedness)의 傾向

박창근,김은희,박매자,배용철,홍해숙,조희중,주강 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.4

This study was investigated the effects of specialization of brain functions, the distribution of handedness and the correlation between cerebral dominance and handedness. This was surveyed with the samples of 1,302 Korean boys and girls attending in junior high school and senior high school, residing in Taegu city. In this study, Alfred W. Munzert's questionnaire and Tames Bliss & Joseph Morella's questionnaire were used as the instrument for assessing the thend of cerebral dominance and handedness. The results obtained were as follows: Out of the total number of 1,302 examined, 73.7% of them were regarded as right hemisphere dominant type and 12.6% of them were regarded as left hemisphere dominant type. In right hemisphere dominant type, students of junior high school were higher incidence than senior high School. In bilateral and left hemisphere dominant type, students of senior high school were higher incidence than junior high school. In right handers most of them were right hemisphere dominant type. In left handers, most of them were right hemisphere dominant type and the incidence of boys were higher than girls.

變壓器의 過渡勵磁電流 豫測法

朴鏞寬,左宗根 成均館大學校 科學技術硏究所 1986 論文集 Vol.37 No.1

Accurate modeling of the transformer, including its saturation characteristics, is an important requirement for studying inrush current, overvoltage and ferroresonance conditions. This paper describes a straightforward method of determining the parameters for a standard T-type equivalent circuit representation of the transformer which can be used to predict the inrush current with good accuracy. The method relies on test measurements that can be readily performed on site: there is no need for design data on the transformer. A comparison of the computed waveforms with measured waveforms of the inrush currents of a distribution transformer is used to verify the accuracy of the method.

Air-coupled 트랜스듀서를 이용한 발전설비 배관에서의 유도초음파 모드 규명

박익근,김현묵,김용권,송원준,조용상,장경영,조윤호 한국비파괴검사학회 2004 한국비파괴검사학회지 Vol.24 No.4

발전설비의 중요한 요소인 배관의 효율적인 비파괴검사를 위해, 배관내에 유도초음파를 comb 트랜스듀서를 이용하여 발생시켰으며, 유도초음파를 비접촉 방식으로 수신하기 위해 ACT(air-coupled transducer)를 적용하였다. comb 트랜스듀서의 요소간격과 이론적인 분산선도로부터 발생가능 한 유도초음파 모드가 예측되었다. 또한 예측된 모드를 수신하기 위해 각 모드의 이론적인 위상속도를 이용하여 ACT의 수신 각도를 결정하였다. 수신모드의 특성을 규명하기 위해 웨이블릿 변환과 2D-FFT를 이용한 시간-주파수해석을 수행하여 이론적인 분산선도와 비교한 결과, 수신된 모드는 이론적으로 예측된 모드와 일치하는 것으로 나타났다. In order to inspect the piping effectively, one of the important components in the facility of power plants, the ultrasonic guided wave was generated by a comb transducer and was received in a non-contact fashion by using an air-coupled transducer. The guided wave modes that can be generated by the comb transducer in piping are predicted from the theoretical dispersion curves and the element spacing of a comb transducer. Moreover, to receive the specific modes, the receiving angle of the air-coupled transducer is calculated from Snell's law between the phase velocities of guided waves and the sound velocity of air. The guided wave modes obtained in experiments are identified from the result of time-frequency analysis such as wavelet transform and two-dimensional fast Fourier transform.