2-Acetylaminofluorene으로 유발된 실험적 간암의 세포 동태학적 연구

금주섭,고영혜,이중달 한양대학교 의과대학 1995 한양의대 학술지 Vol.15 No.2

Kinetics of hepatocytes in various precancerous and cancer iesions during hepatocarcinorenesis was evaluated in 38 Sprague-Dawley rats fed with 0.075% 2-acetylaminofluorene diet. The rats were sacrificed after 4, 7, 10 days. 2, 3, 4, 6, 8 weeks, and 3, 4, 5, 6, 7, 8 months of the experiment. The lesions were divided as altered cell lesions (clear cell focus, acidophilic cell focus, and basophilic cell focus). regenerating nodules. neoplastic nodules, and hepatocellular carcinomas. Cell kinetics was studied by intraperitoneal bromodexyuridine(BrdU) injection 2 hours before sacrifice. After detection of the BrdU-incorporated nuclei with monoclonal antibody kit, the BrdU labelling index(BLI) was measured as percentage of BrdU labelled cells among about 1000 cells. The liver cells in the altered cell lesions showed significantly increased BLI(clear cell 1.66: acidophilic 2.39: basophilic 2.41) in comparion with normal control(0. 18) or non-lesional hepatocytic lobules (0. 47), and lower BLI than hepatocellular carcinoma(8.61∼27.07) (P<0.05). Regenerating and neoplastic nodules did not differ in their BLI (1.54 and 1.70), and were significantly lower in their proliferating kinetics than acidophilic or basophilic cell foci (P<0.05). It was suggested from this study that the altered cell lesion should be true premalignant lesion, which was composed of at least initiated cells by the chemical carcinogen. The basophilic and acidophilic foci in altered cell lesions were suggestive of close correlation with the hepatocellular carcinoma in that they had greatest proliferative cell kinetics of all the lesions occurred before cancer developed.

제왕절개술시 Prostaglandin F₂α 자궁근주가 심혈관계에 미치는 영향

이영주,정금희 아주대학교 의과학연구소 1999 아주의학 Vol.4 No.1

Background : Intramyometrial injection of prostaglandin F₂ α (PGF₂ α), in doses varying from 0.5 to 2 ㎎, has been used as a safe and effective method in the control of severe postpartum hemorrhage due to uterine atony which does not responding to conventional treatment. We earlier reported 8 cases of acute but transient hypertension after myometrial injection of PGF₂ α. The purpose of the present study was to examine cardiovascular consequence of intramyometrial injection of PGF₂ α and incidence of hypertension. Methods : Forty full term pregnant women with no cardiovascular and respiratory diseases, who were scheduled for elective cesarean section under general anesthesia, were randomly grouped into two groups: control (pitocin) and experimental (PGF₂ α) groups. Hypertension developed in five cases after PGF₂ α intramyometrial injection, Thus PGF2 a group was divided into PGF₂ α -normotension (PGF-N) and PGF₂ α -hypertension (PGF-HS) groups, and 20 units of pitocin was infused intravenously in both groups after placental delivery. Twenty unit of pitocin in the control group and 2 ㎎ of PGF₂ α in the PGF₂ α group were directly injected into myometrium, respectively Blood pressure (BP), heart rate (HR) and EKG were continuously monitored, and arterial blood was withdrawn for arterial blood gas analysis. Results : The systotic BP was markedly increased in the PGF-HS group and slightly decreased in the PGF-N group (P< 0.05). The diastolic BP was markedly increased in PGF-HS group, but decreased in the PGF-N and pitocin groups (P< 0.05). The HR was increased at 2 min in the PGF-HS group (P< 0.05). There was no significant difference in ABGA among the groups. Change of EKG occurred in 4 cases only in the PGF-HS group. Conclusions : Only in the PGF-HS group, HR, systolic and diastolic BP are markedly increased and EKG changes are observed. These findings suggest that careful attention should be paid to toxemia as well as to healthy patients when PGF₂ α is used.

뇌사환자의 갑상선 기능, 전신대사 및 중증도에 관한 연구

이영주,정금희,왕희정,문봉기,한연희,이영석 아주대학교 의과학연구소 1999 아주의학 Vol.4 No.1

Background and Objectives : Brain death may lead to hormonal depletion, metabolic derangement and multiple organ dysfunction. We have carried out present study to examine the effects of brain death on the thyroid function, metabolic indices, and the severity scoring systems. Methods : 13 adults patients admitted for organ donation or brain death evaluation were examined after brain death was confirmed. Thyroid hormones measured were .thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and free thyroxine (FT4). The metabolic indices measured were arterial ketone body ratio (AKBR), lactic acid (LA), and base defiat (BD). as for reference to the severity scoring systems, APACHE Ⅲ and multiple organ failure score (MOFS) were assessed on the day of brain death confirmation. Arterial blood was drawn for all measurements. Results : As for the thyroid function, there were significant decreases in T3 (40.48±20.96 ng/dL) and T4 (3.47±2.15 ㎍/dL), but no significant change in FT4 (0.75±0.31 ng/dL) and TSH (1.12±1.37 uIU/mL) compared to the normal range. Significant decreases in AKBR (0.39±0.31) and BD (-9.46±5.83 m㏖/L), and significant increase in LA (2.57±2.46 m㏖/L) In metabolic indices, were shown, as for severity scoring systems, APACHE Ⅲ score (101.54±19.41) and MOF score (9.11±2.57) indicated a high mortality. There were significant correlation between thyroid hormones (r=0.565 -0.781) but no correlation among other indices. Also, significant inverse correlations were shown between base deficit and lactic acid (r=-0.660), APACHE Ⅲ score (r=-0.726) and MOF score (r=-0.604). The highest correlation was observed between APACHE Ⅲ score and MOF score (r=0.851). Conclusions : As for the thyroid function, significant decrease in T3 and T4, and almost normal range of FT4 and TSH imply the euthyroid sick syndrome. Abnormal finding of the metabolic parameters indicates an inhibition of the aerobic metabolic rate of the body as a whole. And the severity scoring parameters are compatible with high mortality.

Stroke에서 감압적 개두술, 정위적 조직 생검과 병리적 소견 고찰 : 중증 제뇌탈출을 동반한 초급성 뇌경색과 신경교종 내 출혈 감별 To differentiate the Herniated hyperacute Cerebral Infarction from the Glioma bleeding

이주용,조준,박호권,장상근,문창택,한혜승,이경룡 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

The authors in this manuscript aimed to provide a practical importance of the differential diagnosis of intracranial brain tumor with emphasis on non-neoplastic abnormalities such as cytotoxic edema of the penumbra zone in strokes. The well known SOLs are cerebral ischemic, inflammatory and dysplastic lesions, which intraaxially imitate glioma and other neurogenic tumors. Brain tumor is the one of common intracranial space-occupying lesions (SOLs) on the diagnostic images. However, many non-neoplastic lesions may be indistinguishable from tumors. Especially, early mortality used to be caused by space-occupying hyperacute ischemic stroke, brain edema and herniations. For the retrospective analysis, the authors reviewed the patient charts, the emergency brain Computerized Tomography (CT), magnetic resonance imaging (MRI), clinical stroke history. From Jan. 2003 to Dec. 2003, we managed total 61 acute strokes in ICU. Even though we operated six decompressive craniotomies, nine stereotactic craniotomies including two procedures of brain biopsy and three extraventricular drainage procedures, three patients (4.91%) were finally expired out from the induced herniation and medullary failure by those aggressive strokes. 43 patients (70.5%) were recovered good from stroke attack. 15 patients (24.6%) were improved with residual neurologic deficits. In conclusion, aside from growing efforts of the radiologist to make the early diagnostic impression, the authors suggest that urgent neurosurgical explorations with the pathologic diagnostic decision should be requested to decrease the fatality.

거리 패션에 나타난 코디네이션 경향 연구 : 1996년 S/S 시즌 서울을 중심으로

허은선,박금주 공주영상정보대학 1998 논문집 Vol.5 No.-

The purpose of this study is to research of the coordination trends in Seoul which is the capital of Korea. The study focuses especially on the spring & summer season of 1996. For the study, 375 photographs were taken at 8 of the more fashionable streets. The results are as follow; 1) May of the styles were revival from the 1960's. For example, clean mode suit hip bone pants, fit & flare line skirt, black & white color. 2) There was also a boom in T-shirts which had the names of foreign universities printed on them most of these were hooded sweat shirts made from terry cloth. This shows the preference for studying at the leading private universities abroad. 3) The fashion styles are now seasonless : temperature is not any longer a conclusive factor for choosing clothes, this season, some winter apparel appeared in fashion styles worn in warm weather. For example, western style boots, shinny white long boots and wide hair band which are influenced by ski wear. This shows that consumers prefer certain fashion items no matter what season it is.

65세 이상 노인 급성 심근경색증 환자의 사망에 영향을 미치는 인자들

정봉기,양금열,손준혁,주시내,남홍우,전용덕,이홍순 대한노인병학회 2001 대한노인병학회지 Vol.5 No.2

한약재의 절단 , 수치 , 전탕법에 관한 연구 Ⅲ : 厚朴 Cortex Magnoliae

金仁洛,黃今熙,朱蕙庭 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

To improve the quality control of frequantly used oriental medicinal plants, the cutting, processing and decokting methods of Magnolia obovata were examined. The contents of magnolol of Magnolia obovata were analysed by HPLC at various conditions as the standard component. The content of magnolol increased about 3% in baked Magnolia obovata. The soaking and steaming processing for easy cutting resulted in the loss of the standard components about 10%. The best decokting conditions which were determined by the yield of magnolol were as follows : the size was below 0.5㎝, soaking time was 0 minutes, decokting time was 75 minutes after boiling.

한약재의 보관 , 절단 , 수치 , 전탕법에 관한 연구 1 : 육계 Cortex Cinnamomi

金仁洛,黃今熙,朱慧庭 대한본초학회 1998 大韓本草學會誌 Vol.13 No.2

To improve the quality control of frequantly used oriental medicinal plants, the stroage, cutting, processing and decokting methods of Cinnamomum cassia were examined. The contents of cinnamic acid and cinnamaldehyde of Cinnamomum cassia were analysed by HPLC at various conditions as the standard components. Standard components decreased in aerobic condition more than in anaerobic storage condition. The cinnamic acid decreased and the cinnamaldehyde increased during the aerobic and anaerobic storage condition. Especialy, cinnamaldehyde was conciderably increased at aerobic storage condition. The soaking and steaming processing for easy cutting resulted in the loss of the standard components. The best decokting conditions which were determined by the yield of cinnamic acid were as follows : the size was below 0.5㎝, soaking time was 90 minutes, decokting time was 15 minutes after boiling.

K대학 치위생과 재학생의 학과만족도 조사 연구

송윤신,권순복,안금선,김영남,김수화,차민주 경복대학 2003 京福論叢 Vol.7 No.-

경기 K대학 치위생과 재학생들의 대학 진학시 학과선택 요인을 알아보고, 학과 만족도를 분석하여 치위생과 학과 경쟁력을 높이고 학생지도의 기초자료를 제공하고자 한다. 치위생과 입학 결정시 영향을 준 요인으로는 전반적으로 본인이 가장 높게 나타났으나 학년별로는 2학년이 77.5%로 가장 높았으며, 치위생과 선택이유로는 취업보장, 주위권유, 적성·흥미 순으로 조사되었다. 치위생과 학과만족도에서 강의실환경에 대해서는 전체 조사대상자 중 9.4%가 만족하다고 응답했고, 실험·실습기자재는 36.0%가 만족한다고 응답하였으며, 학우관계에서는 74.0%, 학과지도방침 38.9%, 치과위생사 직업만족도는 79.4%가 만족하고 있었다. 특히 조사대상자 중 다른 학년보다 2학년 학생들에게서 직업만족도를 제외한 다른 항목의 만족도가 가장 높게 나타나고 있다. 직업만족도는 1학년이 85.4%로 가장 높게 조사되었다. 휴학·자퇴를 고려한 경험유무에 대한 질문에서는 있다고 응답한 학생이 47.8%이었으며, 그 이유로 1학년은 경제적이유를, 2, 3학년은 적성이라고 응답하였다. 입학결정요인과 만족도와의 관계에서는 본인이 입학을 결정했을 때 재학만족도, 직업만족도, 학과지도방침, 전임교수에 만족한다고 응답한 경우가 가장 많았으며 진로계획에서는 취업이 가장 높았다. 학우관계와 만족도와의 관계에서는 학우관계에 만족한다고 응답한 경우 재학만족도, 직업만족도, 학과지도방침에 대한 만족도가 높은 것으로 분석되었다. 구강보건교육이 치위생과 선택에 미친 영향력에 대한 조사에서는 학년별 유의성은 보이지 않았으나, 전체조사대상 중 89.5%가 영향력이 보통이상이라고 응답해 높은 비율을 차지하는 것으로 나타났다. The purpose of this study is to identify the factors affecting selection of a major and the department satisfaction for improving the competitiveness of the dental hygiene department and providing the data for students guidance. The most influential person and factor in selecting a major is the person oneself(77.5% of the second-year students responded to the question) and the percentage of employment. The reaction of satisfaction with the classroom and practice facility indicates 9.4% and 36.0% in favor. 74.0% of surveyees is satisfied with classmates. In satisfaction with a course of department and profession, 38.9% and 79.4% of respondents answer in the affirmative. The second-year students of the respondents shows higher rate than other grades in .satisfaction with items above, except the satisfaction with profession. It appears that the freshman answered the most in that question. From the survey related to the experience of thinking over temporary absence from school or voluntary resignation, 47.8% of surveyees responded they had experienced thinking about that. The freshman answered the reason is financial difficulty and the second- and third-year students selected an aptitude. In the correlation coefficient of determinants for selection of college with the department satisfaction, when the determinant is oneself, the satisfaction with being at college, profession, a course of department, and teaching staff is high. In the correlation coefficient of classmates with department satisfaction, the students responding in the affirmative on classmate satisfaction had a high degree of satisfaction with being at college, profession, and a course of department. The experience of oral health education is not statically significant by grades to select the major, but 89.5% of the students majoring dental hygiene at K-college responded over average.

Purge & Trap-GC를 이용한 의약품 필름코팅 정제 중 잔류용제에 관한 연구

장준식,이명자,소유섭,문춘선,이주헌,박희라,김진숙,강경모,이선옥,방성연,유미자,유문균,금오성,이병욱 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약품은 약물을 생체에 적풋하기 위하여 유효성분의 효과가 언제나 일정하게 확보되고 사응에 편리하도록 만들어지는 것이므로 유효썽분 이외에 약효에 영향을 주지 않는 성분이 첨가되는 경운가 많다. 이 때 사용되는 용매들은 제피의 광택 및 건쪼시간의 단축 등을 위하여 휘발점이 낮을 용매들이 주로 사용되어진다. 본 연구는 의약품 필름코팅정제 중 잔류용매 4종(chlorofonr benzen, trichloro ethylen, 1,4-dioxane)에 대한 변형된 pirge & trap-GC 장치를 이용한 동시분석방법을 개발하였으며, 각 표준품의 RSD 값은 chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69% and 1,4-dioxane 3.41%였다. 또한 시중 유통중인 의약품 50종에 대하여 잔류웅매 양을 측정하였으며, 검출되는 잔류용매는 한 건도 없었다. This study nras carried out to develope the analytical method for the mixture of chlorefonn, benzen, trichloroethylen and 1,4-dioxane simultaneously and determine the remainingorgauic solvents in coating tablets by Purge & Trap-GC. The results were as follouFs ; 1. Chloroform, benzen, trio:tloroethylen and 1,4-dioxane separated by tenax #5 trap by HP-624GC column by terrlperature programming. The peaks were separated completely at retentiontime of 6.88min for chloroform, 8.21min for benzen, 10.38miu for trichloroethylen and 11.95minfor 1,4-dioxane. 2. Standard RSD were individually chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69%and 1,4-diorane 3.41%. 3. 60 samples were not detrcted chloroform, benzen, trichloroethylen and 1,4-dioxane.