Therapeutic Effect of Autologous Activated Platelet-rich Plasma Therapy on Mid-dermal to Full-thickness Burns: A Case Series

Karina Karina,Krista Ekaputri,Johannes Albert Biben,Pritha Hadi,Hubert Andrew,Patricia Marcellina Sadikin 대한성형외과학회 2022 Archives of Plastic Surgery Vol.49 No.3

Although modern medicine hasmade great strides in the management of burn injuries, associated complications such as pain, infection, dyspigmentation, and scarring have yet to be fully dealt with. Although skin grafting and meshing are routinely performed on burn patients, this method poses a risk for adverse effects. Activated autologous platelet-rich plasma (aaPRP), which is increasingly used in the field of plastic surgery, contains growth factors beneficial for wound regeneration. Seven cases of burns with varying severity and conditions that were treated with intralesional subcutaneous injection and intravenous aaPRP are presented and discussed herein. This case series indicates that subcutaneous and intravenous aaPRP is a safe procedure with the potential to be an alternative when skin grafting cannot be done or as an adjunct treatment to skin grafting.

Improving the Viability of Freeze-dried Probiotics Using a Lysine-based Rehydration Mixture

( Karina Arellano ),( Haryung Park ),( Bobae Kim ),( Subin Yeo ),( Hyunjoo Jo ),( Jin-hak Kim ),( Yosep Ji ),( Wilhelm H. Holzapfel ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 한국미생물·생명공학회지 Vol.49 No.2

The probiotic market is constantly continuing to grow, concomitantly with a widening in the range and diversity of probiotic products. Probiotics are defined as live microorganisms that provide a benefit to the host when consumed at a proper dose; the viability of a probiotic is therefore of crucial importance for its efficacy. Many products undergo lyophilization for maintaining their shelf-life. Unfortunately, this procedure may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby impacting their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The optimum lysine concentration of the formulation, designated “Zeta-bio”, was found to be 0.03 M for improving the viability of Lactiplantibacillus plantarum Lp-115 by up to 13.86% and a 7-strain mixture (400B) to 41.99% compared to the control rehydrated with distilled water. In addition, the lysine Zeta-bio formulation notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to the in vitro stress conditions of the simulated gastrointestinal tract passage.

The Role of Garlic in Metabolic Diseases: A Review

Karina G. Perez-Rubio,Miriam Mendez-del Villar,Marisol Cortez-Navarrete 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.7

Garlic (Allium sativum L.) is a popular spice that has been widely used for thousands of years in traditional medicine. Several organosulfur compounds in garlic have been linked to its beneficial effects on health. Evidence from preclinical studies and clinical trials supports garlic's antihypertensive, antidiabetic, antiobesity, and hypolipidemic effects. This study aims to summarize clinical trial evidence regarding the effects of garlic on metabolic diseases and its mechanisms of action.

Morphological and anatomical changes in Lilium cv. Arcachon in response to plant growth regulators

Karina Torres-Pio,G umercindo H . D e la Cruz-Guzmán 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.3

The eff ect of fi ve concentrations of gibberellic acid (GA 3 ), benzyladenine (BA) and paclobutrazol (PBZ) on the morphologyand anatomy of Lilium cv. Arcachon was evaluated. To this end, bulbs were immersed for 24 h with fi ve diff erent concentrations(0, 25, 50, 100 or 200 mg L −1 ) of GA 3 , BA, or PBZ. Then the treated bulbs were immediately sown in 2.5 L potscontaining tezontle. Pots containing bulbs were randomly distributed, kept under greenhouse conditions, and watered withSteiner solution. Morphological and anatomical traits were evaluated at the fl owering phase. The results showed that height(85.3 cm), number of leaves (79.7), and leaf area (743.3 cm 2 ) were not modifi ed by GA 3 , but they diminished in response toBA. In contrast, stems were 80% smaller in response to PBZ but, did not aff ect the number of fl ower blooms. The biomassof bulbs (27.6 g) raised by 32 and 48% in response to BA and PBZ, respectively. The number of vascular bundles and shootphloem area was similar in all treatments, whereas the number of vessel elements increased by 69% when compared withcontrol plants (62 mm 2 vessels). The length of epidermal cells in the leaves decreased between 30 and 48% in response to bothGA 3 and BA, whereas no eff ects were observed in response to PBZ. Mesophyll width was greater by applying PBZ. Finally,PBZ at 25 mg L −1 is enough to reduce the size of stems and improve their appearance as pot plants. Whereas PBZ aff ectssignifi cantly the size of the stems, the BA provoked bud abortion. This research shows in detail how the growth regulatorsmodifi ed the anatomy of the stems and leaves as well as the roots development in Lilium cv. Arcachon plants.

Livelihoods for women in Mindanao : A post-conflict reconstruction approach

Karina R. SANTILLAN 이화여자대학교 아시아여성학센터 2015 Asian Journal of Women's Studies(AJWS) Vol.21 No.1

Mahigit dalawang milyong tao ang nawalan ng tahanan at lumikas dahil sa kaguluhan sa Mindanao noong 2000 hanggang 2009. Sa panandaliang paghupa ng kaguluhan, sila ay bumalik sa kanilang komunidad upang simulan ang proseso ng muling pagtatag ng kanilang buhay. Ihinahayag ng artikulong ito kung ano ang naiambag ng mga babae sa proseso ng pagtatag matapos ang kaguluhan. Ito ay isang pag-aaral kung paano nakatulong ang mga babae sa pamamagitan ng paglahok sa mga gawaing pangkabuhayan. Tinitignan nito ang kinahinatnan ng limang proyektong pangkabuhayan na naisakatuparan sa indanao sa mga taong 2000 hanggang 2010. Sinukat ang saklaw ng ambag ng mga kababaihan sa proseso ng pagtatag sa pamamagitan ng pagtukoy ng mga benepisyong naibigay nila sa kanilang pamilya at komunidad, na siyang naging bunga ng kanilang paglahok sa mga gawaing pangkabuhayan. Pinatotohanan ng artikulong ito na ang paglahok ng mga babae sa iba’t ibang gawaing pangkabuhayan ay nakatulong sa proseso ng pagtatag sa aspetong ekonomiko, sosyal at politikal ng mga komunidad na apektado ng kaguluhan. Ikinikumpara din ng artikulong ito ang paglahok ng mga babae sa mga gawaing pangkabuhayan sa ibang mga paraan ng pagtatag na naisakatuparan ng mga gobiyerno at mga ahensya. Isinusulong nito ang paglaan ng mga gawaing pangkabuhayan na hinihimok ang paglahok ng mga babae bilang isang mahusay ng paraan para maisakatuparan ang proseso ng pagtatag matapos ang kaguluhan. The conflict inMindanao has displaced over two million people in the period 2000 to 2009. As it subsides, the displaced return to their communities and begin the process of reconstruction. This paper studies how women contributed to the post-conflict reconstruction of Mindanao by engaging in livelihood activities. It explores five different livelihood intervention projects implemented in Mindanao between 2000 and 2010. The extent of women’s contribution to post-conflict reconstruction is measured by identifying the benefits gained at household and community levels, generated by women’s livelihood work. I argue that women’s participation in such activities have led to economic, social and political reconstruction of the communities affected by in Mindanao. This paper also compares the women’s livelihoods approach with other reconstruction strategies. It also illustrates that interventions for reconstruction therefore must include livelihood programs that encourage women’s participation, as exemplified by the experience of Mindanao.

Biochemical profile in an infant with neonatal hemochromatosis shows evidence of impairment of mitochondrial long-chain fatty acid oxidation

Karina Lucio de Medeiros Bastos,Caio Robledo Quaio,Fabiana Roberto Lima,Iana Manuelle Araújo,Candice Alves Tavares Araújo,Flávia Balbo Piazzon,Ismael Dale Cotrim Guerreiro da Silva,Gabriel Nuncio Bene 대한간학회 2019 Clinical and Molecular Hepatology(대한간학회지) Vol.25 No.1

Hepatocellular Carcinoma in Children: Liver Resection and Liver Transplantation Outcomes

( Karina Mendoza-gallo ),( Carlos Florez-zorrilla ) 대한간학회 2021 춘·추계 학술대회 (KASL) Vol.2021 No.1

Japanese Perspective on Korean Reunification : An Analysis of Interrelations between Social Identity and Power

Karina Korostelina,Yuji Uesugi 이화여자대학교 국제통상협력연구소 2020 Asian International Studies Review Vol.21 No.1

The paper explores how experts in Japan assess and understand the process and consequences of the unification of the Republic of Korea (South Korea) and the Democratic People’s Republic of Korea (North Korea). Based on the theoretical framework of interrelations between social identity and power, this paper asks how Japanese experts frame the process of Korean unification and evaluate its impact on Japan. The data was collected in Tokyo, Japan, through 37 semi-structured and focus group interviews, then examining these interviews using phenomenological and critical discourse analysis. Analysis of data reveals the existence of four competing narratives rooted in the complex relations between meaning of identity, concepts of power, and Japanese policies toward the unification process. The paper expands the description of two narratives currently present in the existing literature, (1) threat and (2) peace, and introduces two new narratives, (3) democratic processes and (4) restorative justice. The final discussion explores how three groups of factors, (1) regional dynamics, (2) domestic policy, and (3) possible models of unification, influence the prevalence of a particular narrative as well as resulting policies of Japan toward Korean unification.

Transcutaneous electrical nerve stimulation, acupuncture, and spinal cord stimulation on neuropathic, inflammatory and, noninflammatory pain in rat models

Karina Laurenti Sato,Luciana Sayuri Sanada,Morgana Duarte da Silva,Rodrigo Okubo,Kathleen A. Sluka 대한통증학회 2020 The Korean Journal of Pain Vol.33 No.2

Background: Transcutaneous electrical nerve stimulation (TENS), manual acupuncture (MA), and spinal cord stimulation (SCS) are used to treat a variety of pain conditions. These non-pharmacological treatments are often thought to work through similar mechanisms, and thus should have similar effects for different types of pain. However, it is unclear if each of these treatments work equally well on each type of pain condition. The purpose of this study was to compared the effects of TENS, MA, and SCS on neuropathic, inflammatory, and non-inflammatory pain models. Methods: TENS 60 Hz, 200 μs, 90% motor threshold (MT), SCS was applied at 60 Hz, an intensity of 90% MT, and a 0.25 ms pulse width. MA was performed by inserting a stainless-steel needle to a depth of about 4-5 mm at the Sanyinjiao (SP6) and Zusanli (ST36) acupoints on a spared nerve injury (SNI), knee joint inflammation (3% carrageenan), and non-inflammatory muscle pain (intramuscular pH 4.0 injections) in rats. Mechanical withdrawal thresholds of the paw, muscle, and/or joint were assessed before and after induction of the pain model, and daily before and after treatment. Results: The reduced withdrawal thresholds were significantly reversed by application of either TENS or SCS (P < 0.05). MA, on the other hand, increased the withdrawal threshold in animals with SNI and joint inflammation, but not chronic muscle pain. Conclusions: TENS and SCS produce similar effects in neuropathic, inflammatory and non-inflammatory muscle pain models while MA is only effective in inflammatory and neuropathic pain models.

Plants cultivated in Choco, Colombia, as source of repellents against Tribolium castaneum (Herbst)

Karina Caballero-Gallardo,Nayive Pino-Benitez,Nerlis Pajaro-Castro,Elena Stashenko,Jesus Olivero-Verbel 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4

Essential oils (EOs) of eight plants collected in Choco, Colombia, including Piper divaricatum, P. pseudolanceifolium,P. confertinodum, P. diazanum, Ocimum campechianum, Siparuna conica, Mikania micrantha and Hedychiumcoronarium, were analyzed by gas chromatography–mass spectrometry and tested as repellents against Triboliumcastaneum, using the area preference method, after 2 and 4 h exposure. The main components found in EOswere methyl eugenol, trans-β-cariophyllene, methyl eugenol, α-pinene, δ-cadinene, γ-elemene, α-pinene and1,8-cineol, for O. campechianum, P. pseudolanceifolium, P. divaricatum, P. confertinodum, P. diazanum, S. conica,M. micrantha, and H. coronarium, respectively. Best repellent activities were observed for oils fromO. campechianum and P. pseudolanceifolium with mean repellent concentration (RC50) values of 0.00006 and0.0001 μL/cm2 after 2 h, and 0.00003 and 0.0001 μL/cm2 after 4 h, respectively; whereas the least potent wasthat from M. micrantha, with RC50 values of 0.074 and 0.040 μL/cm2 at 2 and 4 h exposure times, respectively. Based on average percentage repellence, oils from P. pseudolanceifolium and O. campechianum were classifiedas Class IV repellents and were better than the commercial repellent IR3535, classified as Class II. These data evidencethe Choco region as an important source of natural repellents with promising commercial opportunities.