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      저자 : Kaliaraj Gobi Saravanan (검색결과 1 건)
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        Investigating biological impact of HAp from goat femur reinforced with Zr–Ag for bone tissue engineering application

        Alagarsamy Karthik,Vishwakarma Vinita,Kaliaraj Gobi Saravanan,Kanagasabai Viswanathan,Ramasamy Sathiskumar 한국세라믹학회 2022 한국세라믹학회지 Vol.59 No.4

        In this study, the diff erent weight percentages of ZrO 2 –Ag-reinforced HAp were prepared by the planetary mill and cal- cinated at 800 °C for 5 h. Synthesized nano-composites evolved the hexagonal phase of HAp in addition to ZrO 2 and Ag peaks. Raman spectroscopy confi rmed the high-intensity band of PO 4 −3 group and major bands of Zr and Ag. Staphylococ- cus epidermidis and Pseudomonas aeruginosa bacterial growth were arrested in HA20Zr10Ag nano-composites evaluated by in vitro antibacterial activity. Hemocompatible behavior of the reinforced HAp revealed that the nanocomposites was non-hemolytic with distinct RBC morphology and this eff ect was more pronounced while increasing ZrO 2 concentration. Biocompatibility studies, such as protein adsorption, cell viability, fl uorescence staining, mineralization, and quantifi cation of BMP-2, were analyzed with MG-63. The nanocomposites have adsorbed a high amount of proteins (~ 300 to 700 μg) from the serum. MTT assay revealed the percentage of the viability of the cells was ~ 70% to 80%. In vitro cytotoxic behavior of MG-63 cells illustrated that HA20Zr10Ag nanocomposites expressed polygonal structure with elongated morphology by Alexa Fluor 488 phalloidin and DAPI staining. Similarly, nano-composites powder proved its mineralization capability through intracellular calcium uptake up to the 5th day of MG-63 incubation. While, BMP-2 expression was slightly higher in HA20Zr10Ag nano-composite at 5th day of incubation. From the results, it is illustrated that HAp with 20 wt% of ZrO 2 and 10 wt% Ag nanocomposites has an important role in osteoblastic bone formation.

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