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To understand systematically the hematological changes including the bone marrow changes in chemotherapeutic agent-induced bone marrow hypoplasia and to define the relationship between hematological and bone marrow scan findings and prognosis of the hypoplasia, CCNU (lomustine) was given orally to 44 rabbits to induce hypoplasia of the bone marrow. And serial changes of peripheral blood and bone marrow findings and (111)In Cl(3)((111)In scan)/(99m)Tc tin colloid bone marrow scan((99m)Tc scan) were checked before and after induction of hypoplasia. With assessment of 28 evaluable rabbits, the following results were obtained: 1) Significant hypoplasia of the bone marrow developed around day 4 of CCNU administration and recovered around day 14(cellularity 51,4±13.5% and 24. 5±14.97o before and after CCNU respectively, p<005). Megakaryocyte count was significantly depressed from 95.67,26% to 36.7$gt;31.82%(P$lt;0.005). M: E ratio was decreased from 162±1.19 to 0, 0.5$lt;0. 43(p±0.005). Shift to left(475), maturation a(40%), naked nucleus and degenerated cells(20%), increase of lymphocytes(47%), monocytes and reticulum cells were also found. 2) The uptake ratio of the 99(m)Tc tin colloid bone marrow scan was markedly increased in contrast to the depression of the bone marrow(4.4±2.12 and 14.1±7.06 before and 4 days after CCNU, respectively, p40. 005). Tc scan uptake ratio was inversely related to the cellularity(r=-0.442, p$lt;0.05) and megakaryocyte number of the bone marrow(r= 0.89, p< 0.01) and peripheral blood granulocyte (r = 0. 54. Pg0.01) and platelet count(r=0.40, p$lt;0.05). There was not significant correlation between (111)In scan uptake ratio and hematologic parameters. 3) The amplitude of the change of the (99m)Tc scan uptake ratio was significantly related to the prognosis of the rabbits with experimentally induced hypoplasia of the bone marrow(dead 5.1±2,67, survivors 2,5±0.96, P<0.01). The change of the (111)In scan uptake ratio was not related to the prognosis of these rabbits. In experimentally induced rrow hypoplasia, morphologic changes in addition to the numerical changes of the bone marrow elements were observed. And serial (99)Tc scan of the bone marrow appears to be helpful assessing the severity and predicting the outcome of bone marrow hypoplasia.
Leukemia is known to be an extremely rare cause of diabetes insipidus. We report a case of diabetes insipidus as a complication of leukemia. An 18 year-old man was admitted because of polyuria polydipsia and fever. Bone marrow aspiration and biopsy revealed the diagnosis of acute erythroleukemia (AML, M6). On the water deprivation test, the diabetes insipidus was confirmed to be of the central type. He was managed with intranasal vasopressin for diabetes insipidus with good response. Induction chemotherapy was administered without response, and the patient died of septicemia and respiratory failure 2 months after initial diagnosis. A short review of the literature related to this subject is also presented.
피틴체 분해효소(파이테이즈) 첨가가 영양소 이용성에 미치는 역할이 사료의 종류에 따라 어떻게 달라지는지 조사하기 위하여, 채란계에 기초사료와 70%의 기초사료와 30%의 대두박, 밀기울, 탈지미강(미강) 및 콘글루텐피드(글루텐)을 각각 혼합한 사료에 파이테이즈를 첨가 급여한 것의 단백질, 에너지, 칼슘 및 인 이용성이 첨가되지 않은 것(대조)과 비교되었다. 배설물중의 질소 화합물을 정량하여 사료 단백질 소화율 또는 에너지 소화율이 계산되었으며 섬유성사료 원료의 에너지 함량은 대치법으로 계산되었다. 첨가된 파이테이즈는 단백질의 외관상 소화율을 유의하게 향상시키지 않았으나 대두박 사료에서 뇨질소의 배설량을 유의하게 증가시켰으며 밀기울, 미강 및 글루텐 사료에서 뇨질소 및 뇨산의 배설량을 감소시키는 경향이 있었다. 그리고 파이테이즈는 사료의 대사에너지값을 증가시키는 경향이 있었으나 밀기울, 미강 및 글루텐 사료에서는 뇨에너지의 배설량을 유의하게 감소시켰다. 한편 파이테이즈는 ME와 MEn 값을 대두박과 콘글루텐피드에서 유의하게 증가시켰으나 밀기울과 탈지미강에서는 증가시키는 경향이 있었다. 또한 칼슘 밸런스는 파이테이즈에 의해서 밀기울, 미강 및 글루텐사료에서 유의하게 증가되었으나 대두박사료에서는 이러한 경향이 없었으며, 인 밸런스는 모든 사료에서 유의하게 증가되었다. 본 실험 성적은 파이테이즈가 영양소 이용성에 미치는 작용은 사료의 종류에 따라 다르다는 것을 나타내었다. The role of dietary phytase supplementation on the utilization of protein, uric acid excretion and energy utilization of wheat bran, rice bran and corn gluten feed was studied in layers fed basal diet and diets containing 70% of basal diet with 30% of soybean meal (SBM), wheat bran(WB), defatted rice bran(RB) and corn gluten feed(GLUTEN), respectively, with or without(Control) the phytase supplementation. Nitrogenous compounds in the excreta, the digestibility of dietary protein and energy, and the energy utilization of the fiber-rich ingredients using a substitution method were determined. Supplemental phytase did not give any significant difference on the apparent digestibility of dietary protein compared with those in control and gave an increasing trend in WB, RB and GLUTEN diets, but increased significantly the excretion of urinary nitrogen(UN) in SBM diet, and showed decreasing tendency for the excretion of UN and uric acid in WB, RB and GLUTEN diets. The phytase tended to increase dietary metabolizable energy values, but decreased significantly urinary energy in birds fed WB, RB and GLUTEN diets, and increased significantly ME and MEn values of soybean meal and corn gluten feed, while gave a tendency to increase the ME and MEn values of wheat bran and defatted rice bran. The phytase also increased significantly retention of calcium in birds fed basal, WB, RB and GLUTEN diets and phosphorus retention in all diets. The result indicated that some substances digested by the dietary phytase may have functions decreasing the excretion of urinary nitrogen in birds fed wheat bran, rice bran and com gluten feed.
PVC membrane, which has been used for membrane of electrolyte sensors, shortened sensor lifetime due to poor adhesion to sensor surface and exhibited difficulty in standardization and mass-production. To overcome these problems, the membrane solution was prepared with neutral carrier, matrix(TEOS:DEDMS=1:3), solvent(ethanol), and a catalyzer(HCl). The fabricated electrolyte sensors showed typical electrical characteristics of MISFET (metal-insulator-semiconductor field-effect transistor). The K-, Ca- and Na-ISFETs showed sensitivity of 53, 2b and 50 mV/decade in wide concentration range, respectively. The response time was about 90 seconds and the drift was 0.05mV/hour. These results suggest that the sol-gel method and the lift-off technique can be applied to formation of membranes and expected to improve mass-productivity, standardzation of the sensors.
This study was aimed to take the available informations about the acute poisoning, its prevention and therapy. This survey was accomplished with acute intoxicated patients in the ER(emergency room) of Chonnam National University Hospital during 42 months from Jan.1986 to June 1989. The intoxicated patients occupied 5.8% of total ER patients (47007 cases). The average of intoxicated patients per day in ER was 2.13. On sex analysis, males occupied 49.3% and females 50.7%. On the motive of poisoning, intoxication by accident occupied 42.1%, by suicide attempt 39.4%, and by the others 18.5%. Poisoning substances included CO poisoning(23.9%), agricultural agents (22.8%), drugs(15.4%), chemicals(14.6), bites, rodenticides and food poisoning. The mortality of intoxicated patients was 8% (216 cases) and the major death-inducing substance was paraquat(39.8%). To decrease and prevent these acute poisonings, we suggest, first, that agricultural agents and psychotropic drugs must be carefully controlled by authorities, second, the informations on the acute poisoning continuously collected, third, poison control center established as soon as possible.
피틴체 분해효소 (phytase : 파이테이즈) 첨가가 영양소 이용성에 미치는 역할이 대두박의 종류에 따라 어떻게 달라지는지 조사되었다. 채란계에 기초사료 및 70%의 기초사료와 30%의 옥수수(옥수수사료), 또는 국산, 중국산, 브라질산 및 인도산 대두박을 각각 혼합한 사료(대두박사료)에 파이테이즈를 첨가급여한 것의 단백질 및 에너지 이용성이 첨가되지 않은 것(대조)과 비교되었다. 배설물중의 질소화합물 및 열량을 정량하여 단백질 소화율 또는 에너지 소화율이 계산되었으며 각종 대두박의 에너지 함량은 대치법으로 계산되었다. 채란계는 기초사료를 급여한 것에 비해서 대두박 사료들을 적게 섭취하였고 사료중 파이테이즈 첨가는 사료섭취량을 증가시켰다. 기초사료와 비교해서 대두박 사료들의 외관상 단백질 소화율은 유의차가 없었으나 낮은 질소밸런스(NB)는 주로 뇨질소(UN) 배설량의 증가에 기인하였다. 첨가된 파이테이즈는, 기초사료와 국산 대두박사료의 NB를 낮추고 이는 주로 UN 배설량의 증가에 그리고 옥수수 사료와 브라질 대두박사료의 낮은 NB는 FN 배설량의 증가에 기인하였으나, 인도산 대두박사료에서는 단백질의 외관상 소화율을 증가시켰으나, UN 배설량 증가로 NB를 감소시켰으며, 중국산 사료의 NB와 FN 및 UN 배설량에는 영향이 없었다. 분에너지(FE), 뇨에너지(UE) 및 뇨산에너지(UAE) 배설량은 대두박 사료들에서 기초사료에 비해서 유의하게 높았으나, 사료중 파이테이즈 참가는 그 대조에 비해서 FE 배설량은 브라질 대두박사료에서 증가시키고 중국 대두박사료에서 감소시켰으며 UE 배설량은 인도 대두박사료에서 유의하게 높였다. 사료에 첨가된 파이테이즈는 국산, 브라질 및 중국산 대두박의 대사에너지 값은 높였으나, 인도산 대두박의 대사에너지 값은 낮추었다. 본 성적은 대두박의 종류에 따라 대두박의 단백질과 에너지 이용성에 미치는 사료중 파이테이즈 첨가의 영향이 다르다는 것을 나타내었다. 따라서 대두박의 종류에 따라 단백질 또는 에너지 이용성을 평가하여 사용하는 것이 경제적인 사료배합과 환경관리에 중요하다는 것을 나타내었다. The role of dietary phytase supplementation on the protein and energy utilization of soybean meals was studied in layers fed basal diet and diets containing 70% of basal diet with 30% of yellow corn (Corn) and soybean meals from Domestic, China, Brazil and India (Soybean meal diets), respectively, with or without (Control) phytase supplementation. Nitrogenous compounds in the excreta, the digestibility of dietary protein and energy, and the energy utilization of the soybean meals using a substitution method were determined. Phytase supplementation increased significantly feed intake in birds fed soybean meal diets. Layer fed soybean meal diets did not show any significant differences on the apparent digestibility of dietary protein, but excreted higher urinary nitrogen(UN) result in the lower nitrogen balance(NB) compared with those fed basal diet. Dietary supplementation of phytase decreased NB in Domestic soybean meal diet due to increase of UN excretion and in Brazil soybean meal diet due to increase of fecal nitrogen(FN) excretion, and increased apparent protein digestibility in Indian soybean meal diet but lowered NB due to increased excretion of UN. Layer fed soybean meal diets increased significantly the excretion of fecal energy(FE), urinary energy(UE) and uric acid energy compared with those fed basal diet. But dietary phytase supplementation increased the excretion of FE in Brazil and UE in Indian soybean meal diets. The supplementation of phytase increased values of ME in Domestic, Brazil and China soybean meals, but decreased in Indian soybean meal. The result indicated that roles of dietary phytase supplementation on the protein and energy utilization were different depend upon the sources of soybean meals.
Surface Plasmon Resonance (SPR) sensor system, has rapid response and high sensitivity, can be applicable for detecting reaction times of many biospecific interactions. A SPR sensor system was constructed to detect the antigen-antibody reactions of salmonella and hlgG (human immunoglobulin G). Sensor chips made of gold thin film were used for detecting biological bindings of antigen and antibody reactions. The antigen and antibody reactions for salmonella and hlgG were carried out with various time intervals to observed characteristics of these reactions using SPR sensor system. The resonance angle shift changes were clearly observed at the time of salmonella or h1gG antibody injection into sample cell since each antibody was self-assembled on gold chip surface of the sensor. It was found that the antibodies of salmonella and hlgG reacted with its sensor chip surface in 10 minutes and 60 minutes respectively. And the antigens of both salmonella and hlgG were bound to its antibody within 1 minute.
A surface plasmon resonance (SPR) sensor system for the determination of sucrose concentration was constructed with a gold thin film sensing chip. The properties of gold thin film are critical factors in exciting surface plasmon resonance phenomena. Therefore in the present paper, the fabrication conditions of gold thin film were investigated to optimize the SPR phenomena. The optimum thickness was obtained as 545 Å with 43.75° resonance angle and good surface roughness limitation, about 3 Å. The linear resonance angle shifts and rapid response were observed from the sucrose concentrations ranged from 0 to 40 wt %.
The changes of plasma renin activity, aldosterone concentration, serum sodium, and potassium levels were studied before and after the water loading followed by diuretics injection. The materials were: 13 non-, 11 normal-, and 11 toxemic pregnancy cases. The plasma renin activity and aldosterone concentration of the cord and postpartum blood were also measured. Following were the results: 1. The plasma renin activity was elevated significantly in normal pregnancy, and slightly in toxemic pregaancy. The serum sodium levels were decreased in pregnancy. 2. The plasma aldosterone concentration was slightly decreased in normal pregnancy, and slightly increased in toxemic pregnancy, however, statistically insignificant. 3. The plasma renin activity of the cord and postpartum blood were lower than those of pregnancy cases. 4. The changes of plasma renin activity after the diuretic administration showed an initial increase, which recovered within 2 hours. These changes were the least in normal pregnancy, and the most in toxemic pregnancy. 5. The changes of plasma aldosterone concentration after the diuretic administration were similar to those of plasma renin activity, although the variations were not so wide.