Genome-wide reorganization of histone H2AX toward particular fragile sites on cell activation

Seo, Jungmin,Kim, Kwoneel,Chang, Dong-Yeop,Kang, Ho-Bum,Shin, Eui-Cheol,Kwon, Jongbum,Choi, Jung Kyoon Oxford University Press 2014 Nucleic acids research Vol.42 No.2

<P>γH2AX formation by phosphorylation of the histone variant H2AX is the key process in the repair of DNA lesions including those arising at fragile sites under replication stress. Here we demonstrate that H2AX is dynamically reorganized to preoccupy γH2AX hotspots on increased replication stress by activated cell proliferation and that H2AX is enriched in aphidicolin-induced replisome stalling sites in cycling cells. Interestingly, H2AX enrichment was particularly found in genomic regions that replicate in early S phase. High transcription activity, a hallmark of early replicating fragile sites, was a determinant of H2AX localization. Subtelomeric H2AX enrichment was also attributable to early replication and high gene density. In contrast, late replicating and infrequently transcribed regions, including common fragile sites and heterochromatin, lacked H2AX enrichment. In particular, heterochromatin was inaccessible to H2AX incorporation, maybe partly explaining the cause of mutation accumulation in cancer heterochromatin. Meanwhile, H2AX in actively dividing cells was intimately colocalized with INO80. INO80 silencing reduced H2AX levels, particularly at the INO80-enriched sites. Our findings suggest that active DNA replication is accompanied with the specific localization of H2AX and INO80 for efficient damage repair or replication-fork stabilization in actively transcribed regions.</P>

Synthesis of Phosphaisocoumarins through Rhodium-Catalyzed Cyclization Using Alkynes and Arylphosphonic Acid Monoesters

Seo, Jungmin,Park, Youngchul,Jeon, Incheol,Ryu, Taekyu,Park, Sangjune,Lee, Phil Ho American Chemical Society 2013 Organic letters Vol.15 No.13

<P>A rhodium-catalyzed cyclization using alkynes and arylphosphonic acid monoesters for the synthesis of phosphaisocoumarins is reported. A number of arylphosphonic acid monoesters were selectively cyclized in high yields with functional group tolerance. In addition, unsymmetrical alkynes are applied in high regioselectivity.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orlef7/2013/orlef7.2013.15.issue-13/ol401407v/production/images/medium/ol-2013-01407v_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ol401407v'>ACS Electronic Supporting Info</A></P>

Nerve cuff electrode using embedded magnets and its application to hypoglossal nerve stimulation

Seo, Jungmin,Wee, Jee Hye,Park, Jeong Hoan,Park, Pona,Kim, Jeong-Whun,Kim, Sung June IOP 2016 Journal of neural engineering Vol.13 No.6

<P> <I>Objective</I>. A novel nerve cuff electrode with embedded magnets was fabricated and developed. In this study, a pair of magnets was fully embedded and encapsulated in a liquid crystal polymer (LCP) substrate to utilize magnetic force in order to replace the conventional installing techniques of cuff electrodes. <I>In vitro</I> and <I>in vivo</I> experiments were conducted to evaluate the feasibility of the magnet-embedded nerve cuff electrode (MENCE). Lastly, several issues pertaining to the MENCE such as the cuff-to-nerve diameter ratio, the force of the magnets, and possible concerns were discussed in the discussion section. <I>Approach</I>. Electrochemical impedance spectrum and cyclic voltammetry assessments were conducted to measure the impedance and charge storage capacity of the cathodal phase (CSC<SUB>c</SUB>). The MENCE was installed onto the hypoglossal nerve (HN) of a rabbit and the movement of the genioglossus was recorded through C-arm fluoroscopy while the HN was stimulated by a pulsed current. <I>Main results</I>. The measured impedance was 0.638?∠?−67.8° kΩ at 1 kHz and 5.27?∠?−82.1° kΩ at 100 Hz. The average values of access resistance and cut-off frequency were 0.145 kΩ and 3.98 kHz, respectively. The CSC<SUB>c</SUB> of the electrode was measured as 1.69 mC cm<SUP>−2</SUP> at the scan rate of 1 mV s<SUP>−1</SUP>. The movement of the genioglossus contraction was observed under a pulsed current with an amplitude level of 0.106 mA, a rate of 0.635 kHz, and a duration of 0.375 ms applied through the MENCE. <I>Significance.</I> A few methods to close and secure cuff electrodes have been researched, but they are associated with several drawbacks. To overcome these, we used magnetic force as a closing method of the cuff electrode. The MENCE can be precisely installed on a target nerve without any surgical techniques such as suturing or molding. Furthermore, it is convenient to remove the installed MENCE because it requires little force to detach one magnet from the other, enabling repeatable installation and removal. We anticipate that the MENCE will become a very useful tool given its unique properties as a cuff electrode for neural engineering.</P>

Effect of Angelica gigas Extracts on Antioxidant Activity and Differentiation of Osteoblastic MC3T3-E1 Cells

Jungmin Seo,Bokyung Kim,Aram Kang,Mi-Hwa Park,Kyungha Choi,Sang-Hyeon Lee 한국식품영양과학회 2016 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Using the Enemy’s Vocabularies

Jungmin Seo The Academy of Korean Studies 2009 THE REVIEW OF KOREAN STUDIES Vol.12 No.3

중국 문화산업정책 분석을 위한 시론적 조망

서정민(Seo, Jungmin),이조영(Li, Zhaoyang) 한양대학교 평화연구소 2014 문화와 정치 Vol.1 No.1

본 논문은 1978년 이후 급속하게 발전하는 중국 문화시장에 대한 중국 공산당의 대응 담론과 정책을 시론적으로 분석한 것이다. 확대되는 문화시장에서 나타날 수 있는 대항담론에 대처하기 위해 중국 공산당은 “금지”와 “발전”이라는 상반된 전략을 통하여 문화산업화를 추진하였다. 문화산업을 경제발전의 일부분으로 보는 관점을 통하여 중국 공산당은 문화시장의 발전전략을 적극적으로 수립하게 되나 문화산업이 공산당의 지배이데올로기에 상반되는 담론형성에 기여할 것에 대비하여 핵심분야에 대한 공산당의 직접적 통제를 유지하게 된다. 이 연구는 이러한 상반된 전략을 개괄적으로 소개하는 동시에 이에 내재된 중국공산당의 딜레마에 대한 시론적인 논의이다. This study is a preliminary analysis of the Chinese Communist Party(CCP)’s discursive and policy reactions to the fast emerging Chinese cultural market since 1978. CCP has developed cultural industries while deploying seemingly contradictory strategies, “prohibition” and “development,” to circumvent any possibility of counter-hegemonic discourses from vibrant cultural market in China. While CCP adopted a developmental strategy for cultural market, it also maintained direct control of core areas of the cultural industry. This study is to introduce these contradictory policies while highlighting the dilemma of CCP in its cultural policies.

통신사업자의 사용자 공통 프로파일을 위한 미들웨어의 성능 측정 방안 연구

서정민(JungMin Seo),이철민(CheolMin Lee),김미연(MiYeon Kim),임미숙(MiSook Lim) 한국통신학회 2007 한국통신학회 학술대회논문집 Vol.2007 No.7

네비게이션을 탈피한 하이퍼미디어 시스템

서정민(Jungmin Seo),김상욱(Sangwook Kim) 한국정보과학회 1995 한국정보과학회 학술발표논문집 Vol.1995 No.2

이슬람국가(IS)의 이중적 이념구축: 동원과 타자화의 양적 분석을 중심으로

서정민 ( Jungmin Seo ) 한국외국어대학교 중동연구소 2019 중동연구 Vol.38 No.1

On April 21, 2019, a series of attacks occurred at churches and hotels in the Sri Lankan capital of Colombo, killing about 250 people. The large-scale terrorist attacks show that the activities of the Islamic State(IS)’s remnants and supporting groups may continue for a considerable time in the future, despite the West's announcement of the disappearance of ISIS in March 2019. Thus, it is urgent to clearly understand the nature and function of IS' ideological Daeshism. The IS organization is a terrorist group and its ideology is radical Islamism. However, unlike past Islamist organizations, the IS has declared itself a state and ruled for a certain period of time. It has also developed and transformed its ideology through the phases of its establishment, expansion, occupation, state declaration, and governance. Therefore, this study looks at IS's ideology from a functionalist perspective attempts. It attempts to demonstrate the hypothesis that IS has been seeking to maintain and expand its organization by using dual ideology of mobilization and otherization. To this end, this paper seeks to empirically understand the manufacturing of the organization's dual ideology through quantitative analysis of the Internet magazine Daviq, the IS' most representative public relations. The frequency of nouns, verbs and adjectives in the magazine will be measured using the KH Coder program. Furthermore, the co-occurrence of words tool in the KH code will be used to understand the duplex ideological manufacturing process of the nouns, verbs, and adjectives.

하이퍼미디어에서 문서 재사용을 위한 키워드의 검색

서정민(Jungmin Seo),김상욱(Sangwook Kim) 한국정보과학회 1994 정보과학회논문지 Vol.21 No.12

하이퍼미디어에서 새로운 도큐먼트를 생성할 때, 이미 생성되어 있는 도큐먼트의 일부와 그 내용이나 구조가 같은 경우가 있다. 기존의 도큐먼트에서 필요한 부분을 질의하고 자동으로 그 부분을 찾아서 사용할 수 있다면, 사용자의 노력을 감소시킬 수 있다. 본 논문에서는 기존의 도큐먼트의 일부분을 재사용하기 위하여 키워드를 검색하는 방법을 제안한다. 사용자가 재사용할 부분을 키워드로 입력하면 그 부분에 해당되는 도큐먼트의 일부분을 자동으로 추출한다. 이렇게 추출된 여러 부분들을 새로 생성하는 도큐먼트에 결함함으로써 하이퍼미디어 도큐먼트를 재사용할 수 있다. 재사용할 노드를 네비게이션과 같은 수작업으로 선택하는 것이 아니라, 노드의 내용에 의하여 자동으로 추출하므로, 사용자는 도큐먼트의 정확한 구조와 내용을 파악하지 않아도 된다. 멀티미디어 편집, 지리 정보 검색, 여러 정보 검색 분야에 응용될 수 있다. In generating new hypermedia document, the document may have the same links and nodes as those of the existing documents. If users inquire some useful parts in document and use them in constructing new hypermedia document, we can reduce effots. This paper provides keywords retrieval strategy for reusing a part of hypermedia document. It proposes an algorithm which extracts reusable part of a document automatically and combines extracted parts, if users provide query. The extraction of a reusable node is done not by hand but automatically using user's query and content of nodes. It can be applied to the multimedia editing, the geographical information retrieval and many information retrieval fields.