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심정보(Jung-Bo Sim),장우석(Ou Sek Chang),박자운(JaWoon Park),김영원(Young Won Kim) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.11
Organic Rankine Cycle (ORC) is an eco-friendly thermodynamic system that can generate useful power using unused thermal energy. The ORC consists of an evaporator, a condenser, a pump and a turbine. Among them, the turbine plays an important role in converting the thermal energy of the working fluid to mechanical energy that drives the generator. Therefore, it is very important to design the turbine and verify its performance. A closed loop system can be used to verify the performance of turbine, but it takes a lot of time and money because it used a real refrigerant. Therefore, in this study, an open loop system was used for the air similarity test with the law of similarity. In order to satisfy the similarity law of the turbo machinery, conditions that satisfy geometric, kinematic, and mechanical similarity were selected using the turbine dimensionless number such as flow coefficient and work coefficient. The selected test conditions were an inlet temperature of 80℃, an inlet pressure of 2.0 bar, a rotation speed of 5,000 rpm, and a pressure ratio of 1.05. Furthermore, the process of selecting the air similarity test conditions was described, and the configuration of the test device and the test method were presented.
Purification and Properties of a Membrane-bound Alcohol Dehydrogenase from Acetobacter sp. HA
유진철,심정보,김형근,전홍성,김성준,Yoo, Jin-Cheol,Sim, Jung-Bo,Kim, Heung-Keun,Chun, Hong-Sung,Kim, Sung-Jin The Microbiological Society of Korea 1994 미생물학회지 Vol.32 No.1
초산 생성 균주인 Acetobacter sp. HA로부터 membrane-bound alcohol dehydrogenase(ADH)를 분리 정제하였다. 세포막 분획과 세포막에서 효소의 용출 그리고 크로마토그라피 방법 등을 적용하여 수율 35%, 153배 정제된 효소를 획득하였다. 정제된 효소의 분자량은 330,000 dalton이었으며, 각각 분자량 79,000과 49,000 그리고 45,000 dalton을 가진 세 종류의 subunit로 이루어져 있었다. 그리고 흡수 스펙트럼의 분석 결과 본 효소에는 cytochrome c가 존재함을 확인할 수 있었다. 본 효소는 메탄올을 제외한 1차 지방족 알코올을 기질로 이용할 수 있었으며, formaldehyde, acetaldehyde 그리고 glutaraldehyde의 경우에도 다소간 기질로 이용될 수 있었다. 본 효소의 에탄올에 대한 $K_m$값은 1.38mM이었으며, 최적 pH와 온도는 각각 5.0~6.0과 32${\circ}C$이었다. 본 효소의 활성은 $V_2O_5$와 $ZnCl_2,\; NiCl_2$같은 금속 이온에 의하여 저해되었다. Membrane-bound alcohol dehydrogenase(ADH) was purified to homogeneous state fron an acetic acid producing bacteria, Acetobacter sp. HA. The enzyme was purified about 153-fold with an overall yield of 35% from the crude cell extract by solubilization and extraction of the enzyme with Triton X-100 and subsequent fractions by column chromatography. Upon sodium dodecyl sulphate-PAGE, the enzyme showed the presence of three subunits with a molecular mass of 79,000 daltons, 49,000, and 45,000 daltons, respectively. Absorption oxidized aliphatic alcohols with a straight carbon chain except for methanol. Formaldehyde, acetaldehyde and glutaraldehyde were also oxidizable substrates. The apparent $K_m$ for ethanol was 1.38mM. The optimun pH and temperature were 5.0~6.0 and 32${\circ}C$, respectively. $V_2O_5$ and heavy metals such as $ZnCl_2\;and\; NiCl_2$ were inhibitory to the enzyme activity.