Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang, Chul Hak,Jun, Gyo,Lee, Dug Keun,Lee, Sang Kyou,Park, Se Yeon,Hahm, Eun Ryeong 생화학분자생물학회 1998 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the conventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang,Chul-Hak,Jun,Gyo,Hahm,Eun-Ryeong,Lee,Sangkyou,Park,Seyeon,Lee,Dug-Keun The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 uncleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Genetic associations of body composition, flexibility and injury risk with ACE, ACTN3and COL5A1polymorphisms in Korean ballerinas

( Jun Ho Kim ),( Eun Sun Jung ),( Chul Hyun Kim ),( Hyeon Youn ),( Hwa Rye Kim ) 한국운동영양학회 2014 Physical Activity and Nutrition (Phys Act Nutr) Vol.18 No.2

Jun Ho Kim, Eun Sun Jung, Chul-Hyun Kim, Hyeon Youn and Hwa Rye Kim. Genetic associations of body composition, flexibilityand injury risk with ACE, ACTN3and COL5A1polymorphisms in Korean ballerinas. JENB., Vol. 18, No. 2, pp.205-214, 2014[Purpose] The purpose of this study was to exam the association of body composition, flexibility, and injury risk to genetic polymorphismsincluding ACEID, ACTN3RX, and COL5A1polymorphisms in ballet dancers in Korea. [Methods]For the purpose of this study,elite ballerinas (n = 97) and normal female adults (n = 203) aged 18 to 39 were recruited and these participants were tested forbody weight, height, body fat, fat free mass, flexibility, injury risks on the joints and gene polymorphisms (ACE, ACTN3, COL5A1polymorphism). [Results]As results, the ACEDD genotype in ballerinas was associated with higher body fat and percentageof body fat than the ACEII and ID genotypes (p< 0.05). In the study on the ACTN3polymorphism and ballerinas, the XXgenotype in ballerinas had lower body weight and lower fat-free mass than the RR and RX genotype (p < 0.005). Also, the meansof sit and reach test for flexibility was lower in the ACTN3XX genotype of ballerinas than the RR and RX genotype of ballerinas(p< 0.05). Among the sports injuries, the ankle injury of the XX-genotyped ballerinas was in significantly more prevalence thanthe RR and XX-genotyped ballerinas (p< 0.05). According to the odd ratio analysis, XX-genotyped ballerinas have the injuryrisk on the ankle about 4.7 (95% CI: 1.6~13.4, p< 0.05) times more than the RR and RX-genotyped ballerinas. Meanwhile, theCOL5A1polymorphism in ballerinas has no association with any factors including flexibility and injury risks. [Conclusion]Inconclusion, ACEpolymorphism and ACTN3polymorphism were associated with ballerinas` performance capacity; COL5A1wasnot associated with any factors of performance of Ballerinas. The results suggested that the ACEDD genotype is associated withhigh body fat, the ACTN3XX genotype is associated with low fat-free mass, low flexibility, and higher risk of ankle-joint injury. [Keyword]ballet, flexibility, body composition, injury risk, ACE, ACTN3, COL5A1

Possible Involvement of Photoperiodic Regulation in Reproductive Endocrine System of Female Olive Flounder Paralichthys olivaceus

Hyun Chul Kim,Chi Hoon Lee,Sung Pyu Hur,Byeong Hoon Kim,Jun Young Park,Young Don Lee 한국발생생물학회 2015 발생과 생식 Vol.19 No.1

This study investigated possible involvement of photoperiodic regulation in reproductive endocrine system of female olive flounder. To investigate the influence on brain-pituitary axis in endocrine system by regulating photoperiod, compared expression level of Kisspeptin and sbGnRH mRNA in brain and FSH-β, LH-β and GH mRNA in pituitary before and after spawning. Photoperiod was treated natural photoperiod and long photoperiod (15L:9D) conditions from Aug. 2013 to Jun. 2014. Continuous long photoperiod treatment from Aug. (post-spawning phase) was inhibited gonadal development of female olive flounder. In natural photoperiod group, the Kiss2 expression level a significant declined in Mar. (spawning period). And also, FSH-β, LH-β and GH mRNA expression levels were increasing at this period. However, in long photoperiod group, hypothalamic Kiss2, FSH-β, LH-β and GH mRNA expression levels did not show any significant fluctuation. These results suggest that expression of hypothalamic Kiss2, GtH and GH in the pituitary would change in response to photoperiod and their possible involvement of photoperiodic regulation in reproductive endocrine system of the BPG axis.

Possible Involvement of Photoperiodic Regulation in Reproductive Endocrine System of Female Olive Flounder Paralichthys olivaceus

Kim, Hyun Chul,Lee, Chi Hoon,Hur, Sung Pyu,Kim, Byeong Hoon,Park, Jun Young,Lee, Young Don The Korean Society of Developmental Biology 2015 발생과 생식 Vol.19 No.1

This study investigated possible involvement of photoperiodic regulation in reproductive endocrine system of female olive flounder. To investigate the influence on brain-pituitary axis in endocrine system by regulating photoperiod, compared expression level of Kisspeptin and sbGnRH mRNA in brain and FSH-${\beta}$, LH-${\beta}$ and GH mRNA in pituitary before and after spawning. Photoperiod was treated natural photoperiod and long photoperiod (15L:9D) conditions from Aug. 2013 to Jun. 2014. Continuous long photoperiod treatment from Aug. (post-spawning phase) was inhibited gonadal development of female olive flounder. In natural photoperiod group, the Kiss2 expression level a significant declined in Mar. (spawning period). And also, FSH-${\beta}$, LH-${\beta}$ and GH mRNA expression levels were increasing at this period. However, in long photoperiod group, hypothalamic Kiss2, FSH-${\beta}$, LH-${\beta}$ and GH mRNA expression levels did not show any significant fluctuation. These results suggest that expression of hypothalamic Kiss2, GtH and GH in the pituitary would change in response to photoperiod and their possible involvement of photoperiodic regulation in reproductive endocrine system of the BPG axis.

소아 난치성 무긴장성발작에서의 Deflazacort의 병용투여 효과

강훈철(Hoon Chul Kang),변지윤(Ji Yoon Byun),고창준(Chang Jun Coe) 대한소아신경학회 2002 대한소아신경학회지 Vol.10 No.1

목적:난치성 무긴장성 발작을 보이는 소아 환자에서 corticosteroid 성분의 deflazacort 병용 투영의 발작 조절 효과와 부작용을 알아보고자 본 연구를 시행하였다. 방법:2000년 6월부터 2000년 12월까지 연세의대 세브란스 병원 소아신경과에 6개월 이상의 무긴장성발작 또는 무긴장성발작을 동반한 Lennox-Gastaut 증후군으로 내원한 환아 중, 항간질제를 두가지 이상 최대 용량으로 투여하였음에도 난치성을 보이는 48명의 환아를 대상으로 전향적 개방형 연구를 시행하였다. 약물 투여 전 4주간의 관찰기 이후 최초 4주간은 deflazacort 2 mg/kg/d를 3회 분복하여 투여하였으며 이후 발작 조절을 관찰하면서 20주간은 deflazacort 2 mg/kg/q.o.d로 총 24주간 투여하였다. 4주 간격으로 간질 발작 빈도와 함께 부작용 여부를 측정하였고 deflazacort의 유효성을 평가하기 위한 주결과 변수로 관찰기 동안과 약물 투여 6개월간의 발작회수감소율의 중앙값을 통계적으로 검정하였으며 보조 결과 변수로 간질 발작 빈도가 50% 이상 감소한 환아들과 발작이 완전히 소실된 환아들의 비율을 조사하였다. 더불어 Lennox- Gastaut 증후군에 동반된 무긴장성발작과 무긴장성발작만을 발현하는 예 간에 발작회수감소율의 차이를 분석하였으며 50% 이상 발작 감소를 보인 반응군과 비반응군 간의 임상 양상의 차이를 비교 관찰하였다. 결과:48명의 환아를 대상으로, 관찰기와 약물투여 6개월 기간 중 발작회수감소율의 중앙값은 42.7%로 통계적으로 유의하게 감소됨을 확인할 수 있었다. Lennox- Gastaut 증후군의 발작회수감소율의 중앙값은 48.9%였으며 무긴장성발작 형태로만 발현하는 경우는 39.3%로 Lennox- Gastaut 증후군에서 다소 효과가 높은 경향이 있었으나 통계적으로 의미는 없었다. 간질 발작 빈도가 50% 이상 감소한 예는 22명(45.8%)이였으며, 간질 발작이 완전히 소실된 경우는 4명(8.3%)이였다. 발작 빈도가 50% 이상 감소한 반응군과 비반응군 간의 임상 양상에 있어 통계적으로 의미 있는 차이는 없었다. 부작용은 20명(41.6%)에서 나타났으며 행동장애가 4명(8.3%),체중 증가와 쿠싱 형상이 16명(33.3%)에서 보였다. 하지만 약물을 중단하지는 않았으며 추적 관찰중이다. 결론:항간질제에 난치성을 보이는 소아 무긴장성발작에서 deflazacort의 병용 투여는 간질 발작 조절에 효과가 있었으며, 부작용으로 경한 행동장애와 쿠싱 형상이 있었으나 약물 투여 중단을 요하지는 않았다. PURPOSE: This is a clinical study to evaluate the efficacy and adverse reactions of deflazacort as adjunctive therapy in childhood intractable atonic seizure including Lennox- Gastaut syndrome. METHODS: This is a clinical prospective, add-on, and open-label study performed for 6 months from Jun. 2000 to Dec. 2000 at the pediatric neurology clinic of Severance Hospital. Subjects were selected according to the following criteria, 1) Patients were diagosed as refractory atonic seizure disorder including Lennox-Gastaut syndrome during more than 6 months, 2) Patients had been on maximal doses of at least 2 anticonvulants including sodium valproate and clonazepam or clobazam. We observed seizure frequency of 4 weeks and 24 week medication period as well as adverse reactions every 4 weeks. Those data were analysed primarily for median seizure frequency reduction rate and other efficacy variables such as responder rate with frequency reduction more than 50% and seizure free rate. We also compared the clinical aspects between responder and non responder group. RESULTS: 48 patients were evaluated for efficacy and adverse reactions. Median seizure frequency reduction rate was 42.7%, responders were 22 patients(45.8%) and seizure free patients were 4(8.3%). In Lennox-Gastaut syndrome, median seizure frequency reduction rate was 48.9% and in atonic seizure only 39.3%. However, there were no statistically significant differences in efficacy. We compared clinical aspects between responder and non responder groups, but couldn't find any difference. The number of patients manifesting adverse reactions was 20(41.6%) in an descending order of frequency, weight gain in 16 patients(33.3%), and irritability in 4 patients(8.3%). CONCLUSION: Deflazacort is believed to be an effective and safe anticonvulsant when used as adjunctive therapy for atonic seizure including Lennox-Gastaut syndrome. However, long term follow up is required to evaluate relapse rate and its adverse reactions.

관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

Cs-137 γ-선 조사장치의 방사선장 해석연구

전재식,채하석,이철영,오장진,이병영,명동범 충남대학교 기초과학연구소 1995 忠南科學硏究誌 Vol.22 No.1

In reference to the ISO-4037 and ANSI N13.11, appropriateness of the shielding and collimating system, and suitability of the irradiation and its environmental conditions of a 20 Ci Cs-137 gamma-ray irradiator installed in Korea Institute of Nuclear Safety(KINS) were investigated. Upon the installation of the irradiator, condition of collimator alignment and virtual beam cross section were examined by use of a portable He-Ne laser. In order to evaluate the fitness of the secondary(scattered) to primary ratio of the collimated gamma-ray beam from the irradiator in light of ISO and ANSI requirement, the measurements of the secondary to primary ratio were performed by use of a cavity type ion chamber which had been calibrated in a calibration laboratory that maintains reliable traceability. For the theoretical assessment of the gamma-ray field the collimated beam were divided into fifteen sectors and for each sector the secondary to primary ratio and the gamma-ray spectrum was simulated by means of Monte Carlo method. The outcomes were compared with the experimental results, and cause of scattering and the existence of alleged modifying factors in the irradiator were examined.

3차원 액체 헬륨에서 양이온의 유동률

전철원 慶南大學校 附設 基礎科學硏究所 1994 硏究論文集 Vol.6 No.-

3차원 액체 헬륨에서 양이온의 유동률에 대한 로톤 기여가 온도의 함수로서 얻어진다. 기존이론과는 다른 T^½요소가 급격히 감소하는 지수항과 함께 나타나며 열 평균 수송 단면적은 Schwarz의 측정치 분석과 비교될 때 1/T에 비례한다.