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본 연구의 목적은 자녀에 의한 피학대 노인들의 경험의 의미와 본질을 파악하는 데 있다. 연구를 위해 현상학적 체험연구방법을 사용하였고, 자료수집을 위해 자녀에 의한 학대를 경험한 6명의 노인과 심층면담을 하였다. 연구문제는 첫째, 자녀에 의한 피학대 노인의 경험은 어떠한가? 둘째, 자녀에 의한 피학대 노인의 경험의 본질은 무엇인가? 이다. 그 결과, 11개의 구성요소와 5개의 본질적 주제를 도출하였으며, <자식을 향한 공허한 몸짓>, <호미로 막을 것을 가래로 막다>, <그래도 자식인 부모의 마음>, <벗어나지 못하는 굴레, 자식>, <실오라기 같은 희망>이라는 본질적 주제를 도출하였다. 본 연구결과를 통해 피학대 노인의 경험에 대한 실제적인 경험을 제공함으로써 피학대 노인에 대한 사회적 관심과 실천적 제언을 하였다. The purpose of this study is to understand the essence and meaning of the experiences of the elderly abused by their adult children. This is a phenomenological study based on in-depth interviews conducted with six elderly people who had experience in being abused by their adult children. The research questions include: (a) what are the experiences of the elderly abused by their adult children? and (b) what is the essence of their experiences? This study derived 11 components and 5 essential themes such as “hollow gesture toward child”, “a stitch in time saves nine”, “parent's heart toward child, anyway”, “bridle not be able to be free from, child”, and “hope like a scrap of thread”. The results of this study provoked social attention and provided practical suggestions for the abused elderly based on the knowledge of their experiences, along with the implication for future research.
Rice, as a model system of monocotyledon plants for genomic studies, is a main staple food for over half of the world population. A rice retrotransposon, Tos17, is active during tissue culture and its ability was wildly used in insertional mutagenesis. In this study we have produced 2,000 non-GM mutants induced by Tos17 in rice. We analyzed >2,000 flanking sequences of newly transposed Tos17 copies by the adaptor-ligation PCR method. The frequencies of Tos17 insertions in the genic and intergenic regions were 60.3% and 36.6%, respectively. We also selected four Tos17 insertion mutant lines for three TF genes which can be considered to be considered to be involved in rice seed development based on expression microarray data: osrem3, osta1, osbhlh1-1, and osbhlh1-2 mutant lines. According to Quadruple 9-mer-based protein binding microarray (Q9-UPBM) experiment, we found that the OsREM3, OsTA1, and OsbHLH1 bound to the ACACCAC, CACGTG, and GTAACA motifs, respectively. In combination of Q9-UPBM, RiceArrayNet analysis, and expression microarray data, we identified 8, 20, and 9 putative target genes of OsREM3, OsTA1, and OsbHLH1, respectively. We have been screening and characterizing the mutations by extensive phenotypic analysis as well as the functional analysis of genes.
( Mi Kyong Joung ), ( Hae Suk Cheong ), ( Jeong A Lee ), ( Soo Youn Moon ), ( Eun Jeong Joo ), ( Young Eun Ha ), ( Doo Ryeon Chung ), ( Jae Hoon Song ), ( Kyong Ran Peck ) 대한내과학회 2008 대한내과학회 추계학술대회 Vol.2008 No.-
Joung,Sug,Kim,Hyang-Mi,Park,Kyong,Mi,Jun,Tae-Ho,Lee,Duk-Ju,Hwang,Sung-Dug,Oh,Jong-Sug,Park,Dae-Geun,Song,Cheol-Ho,Pan,Doil,Choi,Yul-Ho,Kim,Baek,Hie,Nahm,Yeon-Ki,Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
The perturbation of the steady state of reactive oxygen species due to biotic and abiotic stresses in a plant could lead to protein denaturation through the modification of amino acid residues, including the oxidation of methionine residues. Methionine sulfoxide reductases (MSRs) catalyze the reduction of methionine sulfoxide back to the methionine residue. To assess the role of this enzyme, we generated transgenic rice using a pepper CaMSRB2 gene under the control of the rice Rab21 promoter with/without a selection marker, the bar gene. A drought resistance test on transgenic plants showed that CaMSRB2 confers drought tolerance to rice, as evidenced by less oxidative stress symptoms and a strengthened PSII quantum yield under stress conditions, and increased survival rate and chlorophyll index after the re-watering. The results from immunoblotting using a methionine sulfoxide antibody and nano-LC-MS/MS spectrometry suggest that porphobilinogen deaminase (PBGD), which is involved in chlorophyll synthesis, is a putative target of CaMSRB2. The oxidized methionine content of PBGD expressed in E. coli increased in the presence of H2O2, and the Met-95 and Met-227 residues of PBGD were reduced by CaMSRB2 in the presence of dithiothreitol. An expression profiling analysis of the overexpression lines also suggested that photosystems are less severely affected by drought stress. Our results indicate that CaMSRB2 might play an important functional role in chloroplasts for conferring drought stress tolerance in rice
Cu(In,Ga)Se2 (CIGSe) has been proven to be a better candidate as a light absorber layer in thin-film solar cells. However, most processes require high vacuum and high temperature during deposition, which results in significant loss of materials and is not applicable to a flexible substrate. Solution processes often involve low processing temperature and cheap precursor, can be used with flexible substrates, and offer the possibility of roll-to-roll manufacturing, potentially reducing manufacturing costs for the module. Here, we have experimentally investigated the optimum synthesis conditions for CIGSe nanoparticles fabricated by using a facile and a non-vacuum reflux method for low-temperature solution processes. By employing various reflux conditions by changing the temperature of heating mantle, single-phase CIGSe nanoparticles were synthesized at 200 °C. On the other hand, synthesized products with an impure multi-phase were formed at heating mantle temperatures lower than 200 °C. XRD measurements confirmed that the Ga content of the CIGSe nanoparticles increased with increasing heating mantle temperature. In addition, the average diameter of the CIGSe nanoparticles increased with increasing reaction time from 5 min to 30 min at a fixed heating mantle temperature of a 200 °C. The optical band gap is calculated by using ultraviolet-visible (UV-Vis) absorption spectra, decreased from 1.69 eV to 1.29 eV with increasing reaction time due to the increased CIGSe nanoparticles size. From our results, we can conclude that the characteristics of the CIGSe nanoparticles can be effectively controlled by using simple growth conditions, thereby providing many advantages for the fabrication of absorber layers for use in CIGSe solar cells.
Adult-onset Still`s disease (AOSD) is an uncommon autoinfi ammatory disease. Typical features include high spiking fever, evanescent rash, and arthritis or arthralgia. Still`s disease is a facet of juvenile idiopathic arthritis, however AOSD and rheumatoid arthritis (RA) are considered unrelated because clinical presentations and hypothesized pathogenesis are different. To our knowledge a case of RA following AOSD has not been reported yet, and we experienced a case so we report it. A 54-year-old man was admitted for one month of fever. Fever was predominant in the afternoon one or two times a day, reaching 39~40℃ and accompanied with pain on ankles. Initial lab showed elevated ESR and CRP, and serum ferritin level was just above normal. Withempiric antibiotics treatment, further evaluations for fever of unknown origin were performed. Bone marrow biopsy was negative, while several reactive lymph nodeswere seen on PET CT. On 16th hospital day serum ferritin was elevated to 1725. 91 ng/ mL and he had new skin rash on his lower legs, he was consulted for rheumatologic evaluation. RF, anti-CCP, ANA, and ANCA were all negative and he was diagnosed as AOSD. With steroid therapy the symptoms and signs were improved. Steroid was tapered and stopped uneventfully within 3 months. After 6 months of the last visit he revisited with painful swelling of both 2nd and 3rd MCP joints, left 2nd PIP joint, and both 3rd and 4th PIP joints. He had no fever or rash, ferritin level was normal. Insidious onset of joint symptoms, typical symmetrical polyarticular involvement of small joints of hands made us diagnose him as seronegative RA. He has been followed on methotrexate.
This study was done to investigate the relationship between the perception of body image, body weight satisfaction or dietary behavior and self-rated health status in Korean college students. Subjects, 285 college students, were divided into three groups (healthy, normal, and unhealthy) according to the answer for the self-rated health question. Information about demographic status, self-rated health condition, height and weight, perception of body image, satisfaction of body weight, concern for body weight control, dietary behavior, nutritional knowledge, and health-related characteristics collected by a self-reported questionnaire. The proportion of men and women in each group was not significantly different. The academic year, major, experience of nutritional education, and type of residence were not significantly related with self-rated health but the pocket money range was significantly associated (p<0.05) with self-rated health. The proportion of subjects rated their health as unhealthy was the lowest in 210-300 thousand won pocket money range and was increased in less than 210 thousand won or over 300 thousand won pocket money ranges. There were no significant differences for age, height, weight and BMI between the groups. The body image perception and body weight satisfaction levels of healthy group was significantly higher than those of unhealthy group (p<0.01 and p<0.001, respectively), but the level of concern for body weight control in healthy subjects was significantly lower than that in unhealthy subjects (p<0.05). The proportion of subjects reported as healthy was significantly increased with increased frequencies of following food behaviors; weekly use of protein foods (p<0.01), vegetables (p<0.05) and dairy products (p<0.01), and food habits such as “regularity of meal time” (p<0.01), “eating in moderation” (p<0.05), and “eating breakfast” (p<0.001). Overall results suggested that the college students have tended to have a better perception of health when they have better body image perception, body weight satisfaction and dietary behaviors.