Myxococcus stipitatus JW111이 생산하는 Polyene계 항암활성물질

안종웅,Ahn, Jong-Woong 한국응용생명화학회 2002 한국농화학회지 Vol.45 No.2

점액세균의 대사산물을 대상으로 새로운 항암활성물질을 탐색하는 과정에서 해양퇴적물에서 분리한 균주 JW111의 균체 추출액이 인체유래의 각종 암세포에 대해 강한 활성을 나타냄을 발견하고, 먼저 생산균주인 JW111이 Myxococcus stipitatus임을 확인한 후 그 대사산물을 분리, 정제하여 구조결정한 결과, Phenalamide $A_1$, $A_2E, $A_3$임을 밝혔다. 이들은 공시된 감수성 암세포에 대해 모두 우수한 활성을 나타내었으며, $IC_50$의 값은 $0.23{\sim}0.50{\mu}g/ml$이었다. 또한 이들은 Adriamycin의 내성 세포주에 대해서도 감수성 세포주와 동일한 활성을 나타내어 내성을 나타내지 않음이 확인되었다. Phenalamides $A_1{\sim}A_3$ were reisolated as cytotoxic substances from culture broth of Myxococcus stipitatus JW111. The producing strain was isolated from the marine sediment collected off the shore of Geomun Island, Korea. The active principles were extracted from cell mass with acetone and successively purified by silica gel column chromatography, Sephadex LH-20 column chromatography, and finally recycling prep. HPLC. These compounds demonstrated significant cytotoxicity against certain human cancer cells, having $IC_50$ values ranging from 0.23 to 0.50 ${\mu}g/ml$. Moreover, they also inhibited the growth of adriamycin-resistant HCT/ADM human cancer cell line as well as its parent sensitive cell line.

Chivosazole F: 점액세균 Sorangium cellulosum 이 생산하는 다제내성 암세포의 생장억제물질

안종웅(Jong-Woong Ahn),최상운(Sang-Un Choi),서영완(Youngwan Seo),노정래(Jung-Rae Rho) 한국생물공학회 2005 KSBB Journal Vol.20 No.5

Myxococcus stipitatus JW150이 생산하는 세포독성 물질의 분리 및 특성

안종웅(Jong Woong Ahn),이정옥(Chong Ock Lee) 대한약학회 2002 약학회지 Vol.46 No.2

Drug resistance is one of the most significant impediments to successful chemotherapy of cancer. Multidrug-resistance (MDR) is characterized by decreased cellular sensitivity to anticancer agents due to the overexpression of P-gly-coprotein. By employing a resistant subline of HCT15 to adriamycin (CLO2), we undertook the screening for agents which were effective to multidrug-resistant cancer cells. As a result, a myxobacterial strain n7150 was selected for study since an activity against CLO2 cells was discovered in the strain. Cytotoxicity-guided of actionation of the culture broth led to the isolation of cystothiazole A and melithlazole F. The producing organism was identified as Myxococcus stipitatus by taxonomic comparison with type strains of Myxococcus sp. As well as its morphological and physiological characteristics. Cystothiazole A and melithiazole F demonstrated potent cytotoxicity against certain human cancer cells with IC50 values ranging from 0.03∼0.72μg/ml. Both compounds were interestingly as active against drug- resistant sublines CLO2 and CP7O as against the corresponding parental cells.

보문 - 효소 대사산물 : 점액세균 Myxococcus sp. JW154 로 부터 항균물질의 분리 및 식물병원균에 대한 in vivo 활성

안종웅(Jong Woong Ahn),김병섭(Byung Sup Kim) 한국미생물생명공학회 2002 한국미생물·생명공학회지 Vol.30 No.2

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보문 - 효소 대사산물 : Myxococcus stipitatus JW117 이 생산하는 Polyene 계 세포독성 물질의 분리 및 특성

안종웅(Jong Woong Ahn),최상운(Sang Un Choi),권호정(Ho Jeong Kwon) 한국미생물생명공학회 2002 한국미생물·생명공학회지 Vol.30 No.2

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Emission of Greenhouse Gases from Upland Rice and Soybean

신용광,안종웅,고문환,심재천,Shin, Yong-Kwang,Ahn, Jong-Woong,Koh, Mun-Hwan,Shim, Jae-Chun Korean Society of Soil Science and Fertilizer 2003 한국토양비료학회지 Vol.36 No.4

밭벼와 콩 재배지에서 메탄과 아산화질소 배출을 측정하였다. 2001년 4월 28일 폭 30 cm 간격으로 대진벼를 파종하였다. 포장의 물관리는 스프링클러 관개와 천연강우 관개로 하였다. 콩 품종 장엽콩, 태광콩, 황금콩을 2001년 6월 16일에 $60{\times}15cm$ 간격으로 한 구멍에 2알씩 파종하였다. 기체시료는 주 2회씩 채취하여 메탄과 아산화질소를 분석하였다. 밭벼 작기중 메탄 배출은 스프링클러 관개구에서 $3.5kg\;CH_4\;ha^{-1}$ 였었고 천연관개구에서 $-1.7kg\;CH_4\;ha^{-1}$ 였었다. 밭벼 작기중 아산화질소 배출은 스프링클러 관개구에서 $50.8kg\;N_2O\;ha^{-1}$ 였었고 천연관개구에서 $8.3kg\;N_2O\;ha^{-1}$ 였었다. 밭벼 재배지에서 배출하는 온실가스 GWP는 스프링클러 관개구와 천연관개구에서 각각 15,822 및 $2,216kg\;CO_2\;ha^{-1}$ 였었다. 콩 작기중 메탄 배출은 장엽콩 재배구에서 $-14.7kg\;CH_4\;ha^{-1}$ 였었고 태광콩 재배구에서 $-4.3kg\;CH_4\;ha^{-1}$, 황금콩 재배구에서 $4.8kg\;CH_4\;ha^{-1}$ 였었다. 콩 작기중 아산화질소 배출은 장엽콩 재배구에서 $4.7kg\;N_2O\;ha^{-1}$, 태광콩 재배구에서 $4.6kg\;N_2O\;ha^{-1}$, 그리고 황금콩 재배구에서 $3.0kg\;N_2O\;ha^{-1}$ 였었다. 콩 작기중 배출하는 온실가스 GWP는 장엽콩, 태광콩, 황금콩 재배구에서 각각 1,152, 1,323 및 $1,027kg\;CO_2\;ha^{-1}$ 였었다. The emission of methane and nitrous oxide was measured from fields of upland rice and soybean. Rice cultivar, Daejinbyeo, was seeded in a row of 30 cm space in upland soil on April 28, 2001. The fields were irrigated with sprinkler irrigation or natural precipitation. Three soybean cultivars Jangyeobkong, Taekwangkong and Hwangkeumkong, were sown with seeding space of $60{\times}15cm$ on June 16, 2001. Gas samples were collected twice a week during the growth period, and methane and nitrous oxide were analyzed. Methane emission from upland rice field of sprinkler irrigation was $3.5kg\;CH_4\;ha^{-1}$. However, negative emission of methane, $-1.7kg\;CH_4\;ha^{-1}$, was observed in upland rice field of natural precipitation. Total nitrous oxide emission from upland rice field of sprinkler irrigation was $50.8kg\;N_2O\;ha^{-1}$, while the emission in the field of natural precipitation was $8.3kg\;N_2O\;ha^{-1}$. Global warming potentials (GWP) in the upland rice fields of sprinkler irrigation and natural precipitation were 15,822 and $2,216kg\;CO_2\;ha^{-1}$, respectively. Methane emissions from fields of soybean cultivars Jangyeobkong, Taekwangkong and Hwangkeumkong were -14.7, -4.3 and $4.8kg\;CH_4\;ha^{-1}$, respectively. Total nitrous oxide emissions from fields of soybean cultivars Jangyeobkong, Taekwangkong and Hwangkeumkong were 4.7, 4.6 and $3.0kg\;N_2O\;ha^{-1}$, respectively. Total GWPs for soybean cultivars Jangyeobkong, Taekwangkong and Hwangkeumkong were 1152, 1323 and $1027kg\;CO_2\;ha^{-1}$, respectively.

자운영 환원답의 벼 품종간 생육특성과 수량에 관한 연구

이병진,안종웅,황동용,오성환,김준환,김상열,구연충,최진룡,Lee, Byung-Jin,Ahn, Jong-Woong,Hwang, Dong-Yong,Oh, Seong-Hwan,Kim, Joon-Hwan,Kim, Sang-Yeol,Ku, Yeon-Chung,Choi, Zhin-Ryong 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

자운영답에서 벼의 생육특성 등을 조사하여 벼-자운영 작부체계에 적합한 품종의 생리생태적 특성을 구명하고자 영남농업연구소 답작 포장에서 실시한 시험결과를 요약하면 다음과 같다. 1. 자운영 재배답은 무재배답에 비해 이앙 후 환원장애에 의한 초기 활착이 떨어졌으나, 풍미벼, 소비벼, 화영벼가 초장 및 개체군생장률 감소가 적은 편이었다. 2. 부위별 질소함량은 자운영 재배답이 무재배답에 비해 높았고, 반대로 질소흡수량은 단위면적당 건물중이 높았던 자운영 무재배답에서 높았다. 3. 자운영 재배답이 무재배답에 비해 수량이 낮았던 원인은 단위면적당 수수가 적었기 때문이다. 4. 수량에 영향을 미친 수량구성요소로써 자운영 재배답에서는 단위면적당 영화수가, 자운영 무재배답에서는 등숙율이 크게 작용하였다. 5. 쌀의 완전미율과 단백질 함량은 자운영 재배답이 자운영 무재배답에 비해 높게 나타났으며, 식미값은 유의적인 차이가 인정되지 않았다. This study was carried out to comparatively evaluate growth characteristics of rice cultivars under rice-mono (conventional) and rice-Chinese milk vetch (Vetch) cropping system. Six rice cultivars such as Geumobyeo, Pungmibyeo, Hwayeongbyeo, Sobibyeo, Junambyeo and Dongjinbyeo were tested in 2005. The results obtained are summarized as follows ; plant height and culm length of all the tested cultivars at heading and harvest time were shorter in rice-vetch cropping system than conventional, however, nitrogen content of rice plants at heading and harvesting time was higher in rice-vetch cropping system. The total amount of nitrogen of rice plants was higher in conventional than rice-vetch cropping system. Nitrogen content of rice plant and uptake of Pungmibyeo was the highest of all the tested cultivars in both cropping systems. Generally, grain yietd ef tested cultivars seemed to be higher in conventional than rice-vetch cropping system. And it was the highest in Junambyeo as compared to those of other cultivars. Head rice ratio and protein content were higher in rice-vetch cropping system.

Seasonal Change of Rice-mediated Methane Emission from a Rice Paddy under Different Water Management and Organic Amendments

신용광,이양수,안종웅,고문환,엄기철,Shin, Yong-Kwang,Lee, Yang-Soo,Ahn, Jong-Woong,Koh, Mun-Hwan,Eom, Ki-Cheol Korean Society of Soil Science and Fertilizer 2003 한국토양비료학회지 Vol.36 No.1

물관리와 유기물 시용이 다른 논에서 메탄 배출을 측정하였다. 벼 식물체를 통해 배출되는 메탄을 측정하기 위하여 벼를 심은 chamber와 심지 않은 chamber를 반복으로 포장에 설치하였다. 기체시료는 벼 재배기간중 주 1회 채취하였다. 상시담수에서는 벼를 심은 NPK구, NPK(+P),는 $0.174g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였으나 심지 않은 NPK구, NPK(-P),는 $0.046g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였다. 벼를 심은 볏짚퇴비 시응구, RSC(+P), 는 $0.214g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였으나 심지 않은 볏짚퇴비 시용구, RSC(-P),는 $0.076g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였다. 볏짚을 2월에 시용하고 벼를 심은 시험구, RS2(+P), 는 $0.328g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였으나 볏짚을 2월에 시용하고 벼를 심지 않은 시험구, RS2(-P),는 $0.1g\;CH_4\;m^{-2}\;d^{-1}$의 배출을보였다. 볏짚을 5월에 시용하고 벼를 심은 시험구, RS5(+P). 는 $0.414g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였으나 볏짚을 2월에 시용하고 벼를 심지 않은 시험구, RS5(-P),는 $0.187g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였다. 간단관개에서는 NPK(+P)는 $0.115g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였으나 NPK(-P)는 $0.041g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였다. RSC(+P)는 $0.137g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였으나 RSC(-P)는 $0.06g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였다. RS2(+P) 는 $0.204g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였으나 RS2(-P)는 $0.09g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였다. RS2(+P)는 $0.273g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였으나 RS5(-P)는 $0.13g\;CH_4\;m^{-2}\;d^{-1}$의 배출을 보였다. 상시담수 처리에서 벼 식물체를 통한 메탄 수송은 NPK구, RSC구 (볏짚퇴비를 5월에 시용한 구), RS2구(볏짚을 2월에 시응한 구)와 RS5구 (볏짚을 5월에 시용한 구)에서 각기 73.6 %, 64.5%, 69.5%, 54.8%였었고 평균 65.6%였었다. 간단관개처리에서 벼 식물체를 통한 메탄 수송은 NPK구, RSC구, RS2구와 RS5구에서 각각 64.3, 59.2, 55.9, 52.4였다. Methane emission was measured in a rice paddy under different water management and organic amendments. Methane emission from planted chambers and unplanted chambers was monitored to evaluate the rice-mediated methane emission. In flooding methane emission from planted chambers with NPK, NPK(+P), was $0.174g\;CH_4\;m^{-2}\;d^{-1}$ while that from unplanted chambers with NPK, NPK(-P), was $0.046g\;CH_4\;m^{-2}\;d^{-1}$ Methane emission from planted chambers with rice straw compost amendment, RSC(+P), was $0.214g\;CH_4\;m^{-2}\;d^{-1}$, while that from unplanted chambers with rice straw compost amendment, RSC(-P), was $0.076g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from planted chambers with rice straw amendment in Fehruary, RS2(+P), was $0.328g\;CH_4\;m^{-2}\;d^{-1}$, while that from unplanted chambers with rice straw amendment in February, RS2(-P), was $0.1g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from planted chambers with rice straw amendment in May, RS5(+P), was $0.414g\;CH_4\;m^{-2}\;d^{-1}$, while that from unplanted chamhers with rice straw amendment in May, RS5(-P), was $0.187g\;CH_4\;m^{-2}\;d^{-1}$. In intermittent irrigation methane emission from NPK(+P) was $0.115g\;CH_4\;m^{-2}\;d^{-1}$, while that from NPK(-P) was $0.041g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from RSC(+P) was $0.137g\;CH_4\;m^{-2}\;d^{-1}$, while that from RSC(-P) was $0.06g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from RS2(+P) was $0.204g\;CH_4\;m^{-2}\;d^{-1}$, while that from RS2(-P) was $0.09g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from RS5(+P) was $0.273g\;CH_4\;m^{-2}\;d^{-1}$, while that from RS5(-P) was $0.13g\;CH_4\;m^{-2}\;d^{-1}$. Methane transport via rice plant under flooding for NPK plot, RSC plot, RS2 plot and RS5 plot was 73.6%, 64.5%, 69.5% and 54.8%, respectively, and mean was 65.6%. Methane transport via rice plants under intermittent irrigation for NPK plot, RSC plot, RS2 plot and RS5 plot was 64.3%, 59.2%, 55.9% and 52.4%, respectively, and mean was 58.0%.

Epi-xanthatin의 Side Chain 변환을 통한 새로운 반합성 유도체들의 합성 및 세포독성

백두종(Du-Jong Bae),안종웅(Jong-Woong Ahn),이정옥(Chongock Lee) 大韓藥學會 2005 약학회지 Vol.49 No.1

Epi-xanthatin analogs containing hydrophilic substituents such as carboxylic acid, alcohol, morpholine, amino acid, and glucose derivatives were synthesized and their in vitro cytotoxicity and in vitro antitumor activity were evaluated. The target compounds were generally cytotoxic against tumor cell lines of human origin with ED so values of 0.1-30㎍/ml, except the highly hydrophilic analog 6 containing aspartic acid. Contrary to the potent cytotoxicity, weakly hydrophilic analogs 2 and 8 were not active in vivo, or even toxic to the test animals. As a result, hydrophilic analog of epi-xanthatin did not show in vitro cytotoxicity and hydrophobic analogs did not show in vivo antitumor activity thus it is presumed that amphiphilic analogs or those with medium hydrophilicity would exhibit the antitumor potency in vivo.

Effect of Rice Vegetation and Water Management on Thrnover of Incorporated Organic Materials to Methane in a Korean Paddy Soil

신용광,김건엽,안종웅,고문환,엄기철,Shin, Yong-Kwang,Kim, Gun-Yeop,Ahn, Jong-Woong,Koh, Mun-Hwan,Eom, Ki-Cheol Korean Society of Soil Science and Fertilizer 2003 한국토양비료학회지 Vol.36 No.1

Turnover rate of applied rice straw and rice straw compost in a rice paddy soil under the flooding and intermittent irrigation was studied. The chambers in duplicate were either planted or unplanted with rice. For planted chambers, the turnover rate to methane under the flooding increased with freshness of organic materials applied: 14.9% for RS5 (rice straw applied in May), 9.5 % for RS2 (rice straw incorporated in February), and 4.0% for RSC (rice straw compost amended in May). Similar trend was found in turnover rate to methane under the intermittent irrigation: 9.8% for RS5, 5.5% for RS2, and 2.1% for RSC. For planted chambers, turnover rate to methane under the flooding was generally 1.64 times higher than that under the intermittent irrigation. In contrast, for unplanted chambers, the turnover rate to methane under the flooding increased with freshness of organic materials applied: 8.7% for RS5, 3.3% for RS2, and 3.0% for RSC. Similar trend was observed in chambers under the intermittent irrigation: 5.4% for RS5, 3.0% for RS2, and 1.4% for RSC. For unplanted chambers, the turnover rate to methane under the flooding was generally 1.52 times higher than that under the intermittent irrigation. 논에 볏짚과 볏짚퇴비를 시용하고 상시담수와 간단관개로 관리하면서 벼가 심긴 chamber와 벼가 심겨지지 않은 chamber에서 메탄으로 전환되는 정도를 조사하였다. 벼가 심긴 chamber에서는 상시담수에서 메탄 전환율은 시용한 유기물의 신선도에 따라서 증가하였다. 볏짚을 5월에 시용한 처리 (RS5), 볏짚을 2월에 시용한 처리 (RS2), 볏짚퇴비를 5월에 시용한 처리 (RSC)에서 전환율이 각기 14.9%, 9.5%, 4.0%였었다. 범위는 4.0% ~ 14,9 %, 평균은 9.5%였었다. 간단관개에서 전환율도 유사한 경향이었다. 간단관개한 RS5 처리, RS2 처리, RSC 처리에서 메탄으로 전환율을 각기 9.8%, 5.5%, 2.1%였었다. 범위는 2.1% ~ 9.8%, 평균은 5.8%였었다. 벼를 심은 chamber에서 상시담수에서 메탄으로 전환율은 간단관개에서 보다 1.64배 높았다. 벼를 심지 않은 chamber에서 상시담수에서 메탄 전환율은 시용한 유기물의 신선도에 따라서 증가하였다. RS5 처리 RS2 처리 RSC 처리에서 전환율이 각기 8.7%, 3.3%, 3.0%였었다. 범위는 3.0% ~ 8.7%, 평균은 5.0%였었다. 간단관개에서 전환율도 유사한 경향이었다. 간단관개한 RS5 처리, RS2 처리. RSC 처리에서 메탄 전환율을 각기 5.4%, 3.0%, 1.4 %였었다. 범위는 1.4% ~ 5.4%, 평균은 3.3%였었다. 벼를 심은 chamber에서 상시담수에서 메탄 전환율은 간단관개에서 보다 1.52배 높았다.