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잡초성벼의 superoxide dismutase cDNA cloning과 재배벼로의 형질전환
박상규,박종석,이승인,서석철,김병극,조윤래,서학수 한국환경농학회 2002 한국환경농학회지 Vol.21 No.2
냉해나 한발등의 환경스트레스에 대해 저항성을 유발하는 유전자를 환경스트레스에 강한 잡초성벼로부터 선발하고 이들 유전자를 재배벼에 도입하여 도입유전자 산물의 과량 발현을 통해 냉해나 한발 등에 대한 저항성이 향상된 벼를 선발하고자 하였다. 잡초성벼인 Bhutan 14Ad로부터 냉해 및 한발 저항성 유전자로 알려진 superoxide dismutase (SOD) cDNA를 분리하고자 mRNA를 분리하고 이 분리된 mRNA를 이용해 reverse transcriptase PCR방법으로 SOD cDNA를 cloning 하였다. 그 결과 2종의 SOD cDNA가 cloning되어 SOD-A, SOD-B로 명명하였다. 이들 cDNA의 염기서열을 결정한 결과 이들은 아미노산 서열 상동성이 88.4%를 나타내었으며, SOD-A는 oryza sativa 계열의 Cu/Zn SOD유전자인 GenBank accession No. L36320와 99.3%로 동일하였으며, SOD-B는 accession No. D01000과 100% 동일하였다. 이들 SOD-A와 SOD-B cDNA를 재배벼인 낙동벼에 형질전환하여 형질전환체 벼를 선발하였으며, 이들 형질전환체 벼의 냉해저항성 및 한발저항성 검정을 통해 저항성이 향상된 형질전환체 벼를 선발하고 있다. Two different cDNA clones for superoxide dismutase (SOD) were isolated from an weedy rice variety (Oryza sativa, cv. Bhutanl4Ad) and were introduced into a cultivated rice variety (Oryza sativa, cv. Nakdong) in order to develop the environmental stress-resistant rice plants. Sequence analysis of the cloned cDNAs indicated that the deduced amino acid sequence of SOD-A is 88.4% identical to that of SOD-B. Furthermore, the nucleotide sequence of SOD-A is 99.3% identical to that of a Cu/Zn SOD gene of Oryza sativa (GenBank accession No. L36320). The nucleotide sequence of SOD-B was identical to that of the previously published SOD gene (Accession No. D01000). A cultivated rice variety, Nakdong-byeo, was transformed with chimenc SOD genes containing a actin promoter of rice and pin2 terminator using a particle bombardment technique. Transformed calli were selected on an selection medium containing phosphinothricin (PPT). Transgenic rice plants were regenerated from the PPT-resistant calli. PCR analysis with genomic DNAs from transgenic plants revealed that transgenes are introduced into rice genome.
최민호,이계복,조규원,박진영,김경수,송정석,한종현 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1
The purpose of this study is to measure the changes of regional cerebral blood flow (rCBF) and blood pressure (BP) of Cortex Acanthopanacis in rats, and to determine the effect of Cortex Acanthopanacis on phenylephrine (PE) induced contraction of isolated rat thoracic aorts. The measurement was continually monitored by laser-doppler flowmeter (Transonic Instrument, USA) and pressure transducer (Grass, USA) in anesthetized adult Sprague-Dawley rats through the data acquisition system composed of MacLab and Macintosh computer. Contractile force was measured with force displacement transducer under 1.5 g loading tension. The result of this experiment was as following; 1. Cortex Acanthopanacis did not effect the changes of rCBF and blood pressure significantly. 2. Contractions evoked by phenylephrine were decreased significantly by Cortex Acanthopanacis 3. L-NNA, ODQ, atropine and indomethacin significantly altered the relaxation of Cortex Acanthopanacis. 4. Propranolol did not change the relaxation of Cortex Acanthopanacis. These results indicate that Cortex Acanthopanacis did not change the rCBF and BP, but Cortex Acanthopanacis can relax PE induced contraction of isolated rat thoracic aorta and that this increasing contraction related to endothelium and various mechanism.
Jong-Gyu Hwang,Jong-Hyun Baek,Hyun-Jeong Jo,Kang-Mi Lee 제어로봇시스템학회 2014 제어로봇시스템학회 국제학술대회 논문집 Vol.2014 No.10
In accordance with the increase in importance of software within railway signaling system, risk costs due to the software error are increasing too. Accordingly, software validation in various aspects is required, and the needs for supporting tool which can automate it are raised. In Korea, the black-box testing tool of railway signaling system taking into consideration of the convenience of use which was developed to solve demerits of existing commercial tools was proposed and developed recently. This tool is an approach which generates test data on the basis of the international standard test language, TTCN-3(Testing and Test Control Notation-3), and utilizes interfaces for actual use, and its convenience of use was enhanced in comparison with existing tools, and more effective test is possible with it. This paper describes the system-level black-box testing process on the on-board control system which is one of the railway signaling systems and its result by utilizing this tool. In addition, it considers effects of study and direction of development in the future on the basis of its result.
Software Black-box Testing Tool for Railway Signaling System by Real Interface
Jong-Gyu Hwang,Jong-Hyun Baek,Hyun-Jeong Jo,Kang-MI Lee 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10
The railway signaling system is being converted to the computer system from the exiting electrical equipment, and then the dependency on software is being increased. So far, the software validation has been mainly dependent profess, the these methodology is just depended to software experts as a tester. So it is our situation where not only validation of the document on safety activities being required, but also another automatci testing tool for vital railway signaling software. There were difficult to take advantage at the existing software black-box testing tool like usage of internal memory access of target system. This paper have proposed a new black-box testing tools with enhanced usability and the represents the design and prototype result for proposed tool.
黃宗奎(Jong-Gyu Hwang),李在浩(Jae-Ho Lee),趙賢庭(Hyun-Jeong Jo),李鐘宇(Jong-Woo Lee) 대한전기학회 2006 전기학회논문지 B Vol.55 No.9
According to the computerization of railway signaling systems, the interface link between the signaling systems has been replaced by the digital communication channel. At the same time, the importance of the communication link is more pronounced than before. In this paper, new Network-based protocol between railway signaling and SCADA (Supervisory Control and Data Acquisition system) has designed and the overview of designed protocol is briefly represented. And also this paper addresses an analysis of newly designed train control systems. Fame error rates of the data transmissions are calculated and compared for the two cases that the CTC (Centralized Traffic Control)/SCADA has an extra data transmission error control (CRC16) besides the inherent error control of the Ethernet and that the CTC/SCADA has no extra data transmission error control. With simulation results it has been verified that the additional error control code contributes to lowering the frame error rate. It will be expected to increase the safety, reliability and efficiency of maintenance of the signaling systems by using the designed protocol for railway signaling system.
철도신호시스템 상호운용성 검증 방법 및 지원도구의 개발
황종규(Jong-Gyu Hwang),조현정(Hyun-Jeong Jo),백종현(Jong-Hyun Baek) 한국산학기술학회 2015 한국산학기술학회논문지 Vol.16 No.9
Railway signaling system is the vital control system in charge of the core for safely train operation, its functional safety must be checked through sufficient verification. Until now, to verify the interoperability of developed railway signaling system, the on-site testing approaches have been applied after testing in laboratory with simulator. This approaches are some limited range of interoperable verification, so ㅡore systematic verification interoperability is required. In this paper, the three phases of interoperability verification for railway signaling system was proposed, and methodology for each phases are represented. And also The interoperability validation methodology and supported testing tool for railway signaling system is represented in this paper
IEC 62280을 통한 철도신호용 표준 통신프로토콜의 안전특성 분석
황종규(Jong-Gyu Hwnag),조현정(Hyun-Jeong Jo),윤용기(Yong-Ki Yoon),김용규(Yong-Gyu Kim) 한국철도학회 2006 한국철도학회 학술발표대회논문집 Vol.- No.-
The existing Europe Standard, EN 50159 with respect of safety of railway signalling communication protocol has been changed into International Standard, IEC 62280. This Standard presents the requirement for safe communication between safety-related devices which connects with transmission system, there are both closed and open transmission system. Regardless of this international Standards, the communication protocol for interface between CTC communication server and external facilities has been standardized several years ago in our country, so this is applied to integrated CTC system in KORAIL. Two Standards are currently standardized as a protocol between CTC and external facilities, these standard protocols are also required high level safety as a communication link which is transmitted vital control information in common with the train control system. For that reason, we have made analysis of requirement for the safe transmission required by international standard. Under this analysis, we have studied safety features of communication protocol applying to our standard. In other words, we have researched that how many the requirement from international standard for safe transmission is reflected in domestic standard protocol, and also whether our standard makes sure of safety enough or not.