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The Ciliary Transition Zone: Finding the Pieces and Assembling the Gate
João Gonçalves,Laurence Pelletier 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.4
Eukaryotic cilia are organelles that project from the surface of cells to fulfill motility and sensory functions. In vertebrates, the functions of both motile and immotile cilia are critical for em-bryonic development and adult tissue homeostasis. Importantly, a multitude of human diseases is caused by abnormal cilia biogenesis and functions which rely on the com-partmentalization of the cilium and the maintenance of its protein composition. The transition zone (TZ) is a specialized ciliary domain present at the base of the cilium and is part of a gate that controls protein entry and exit from this organelle. The relevance of the TZ is highlighted by the fact that several of its components are coded by ciliopathy genes. Here we review recent developments in the study of TZ proteomes, the mapping of individual compo-nents to the TZ structure and the establishment of the TZ as a lipid gate.
Adriana Hamond Regua-Mangia,Alice Gonçalves M. Gonzalez,Aloysio M. F. Cerqueira,João Ramos C.Andrade 대한수의학회 2012 Journal of Veterinary Science Vol.13 No.2
Escherichia (E.) coli serotype O157:H7 is a globally distributed human enteropathogen and is comprised of microorganisms with closely related genotypes. The main reservoir for this group is bovine bowels, and infection mainly occurs after ingestion of contaminated water and food. Virulence genetic markers of 28 O157:H7 strains were investigated and multilocus enzyme electrophoresis (MLEE) was used to evaluate the clonal structure. O157:H7 strains from several countries were isolated from food, human and bovine feces. According to MLEE, O157:H7 strains clustered into two main clonal groups designated A and B. Subcluster A1 included 82% of the O157:H7 strains exhibiting identical MLEE pattern. Most enterohemorrhagic E. coli (EHEC) O157:H7 strains from Brazil and Argentina were in the same MLEE subgroup. Bovine and food strains carried virulence genes associated with EHEC pathogenicity in humans.
de Araújo Amanda Dias,de Barros Pimentel Maria do Carmo,Santos Camila da Silva,da Silva Roberto Afonso,Cadena Pabyton Gonçalves,da Silva Nicácio Henrique,Gusmão Norma Buarque,Sleifer Bruno Alonso,da S 경희대학교 융합한의과학연구소 2021 Oriental Pharmacy and Experimental Medicine Vol.21 No.4
The focus of this study was to evaluate the presence and type of carbohydrates and phenolic molecules inaqueous extract (Aq-E) of Alternanthera brasiliana fresh leaves and its antioxidant and antimicrobial activities and cytotoxicity in vitro. The amount and types of the carbohydrates were measured by colorimetric and TLC methods. Phenolic compounds was detected by colorimetric assay and analysed by HPLC profiles. The antioxidant activity was evaluated by ABTS and Phosphomolybdenum methods. Antimicrobial activity was tested by microdilution method using microorganism models and antibiotic as positive control, the cytotoxicity in vitro was tested using Artemia salina. The results showed the presence of high amount of total sugars and uronic acids. TLC chromatograms showed mainly D-glucose, D-fructose, oligosaccharides and uronic acids in the Aq-E and a sugar alcohol in the methanolic extract. The colorimetric determination showed high concentration of phenolic compounds, which were visualised on the HPLC profiles, such as chemical markers of the Amaranthaceae family and several phenolic acids and flavonoids. The Aq-E demonstrated optimal antioxidant activities. The most important results were the excellent antibacterial and bactericidal activities against Mycobacterium megmatis (MIC = 15.6 µg/mL and MBC = 1000 µg/mL) and antimicrobial activity against Candida albicans (MIC = 31.2 µg/mL) and low cytotoxicity. Further possibilities for this plant extract will be to improve the pharmacological potential for developing new herbal medicines and possibly to study its association to allopathic antibiotics for prevention or treatment of infection diseases.
Emmanuel João Nogueira Leal Silva,Felipe Gonçalves Belladonna,Marianna Fernandes Carapiá,,Brenda Leite Muniz,Mariana Santoro Rocha,Edson Jorge Lima Moreira 대한치과보존학회 2018 Restorative Dentistry & Endodontics Vol.43 No.1
Objectives This study evaluated the amount of remaining root canal filling materials after retreatment procedures performed by undergraduate students using manual, rotary, and reciprocating techniques through micro-computed tomographic analysis. The incidence of instrument fracture and the instrumentation time were also evaluated. Materials and Methods Thirty maxillary single rooted teeth were prepared with Reciproc R25 files and filled with gutta-percha and AH Plus sealer by the continuous wave of condensation technique. Then, the specimens were assigned to 3 groups (n = 10), according to the retreatment technique used: manual, rotary, and reciprocating groups, which used K-file, Mtwo retreatment file, and Reciproc file, respectively. Retreatments were performed by undergraduate students. The sample was scanned after root canal filling and retreatment procedures, and the images of the canals were examined to quantify the amount of remaining filling material. The incidence of instrument fracture and the instrumentation time were recorded. Results Remaining filling material was observed in all specimens regardless of the technique used. The mean volume of remaining material was significantly lower in the Reciproc group than in the manual K-file and Mtwo retreatment groups (p < 0.05). The time required to achieve a satisfactory removal of canal filling material and refinement was significantly lower in the Mtwo retreatment and Reciproc groups (p < 0.05) when compared to the manual K-file group. No instrument fracture was observed in any of the groups. Conclusions Reciproc was the most effective instrument in the removal of canal fillings after retreatments performed by undergraduate students.
Bruno Augusto Alves Martins,Marcelo de Melo Andrade Coura,Romulo Medeiros de Almeida,Natascha Mourão Moreira,João Batista de Sousa,Paulo Gonçalves de Oliveira 대한대장항문학회 2017 Annals of Coloproctolgy Vol.33 No.3
Transanal endoscopic microsurgery is considered a safe, appropriate, and minimally invasive approach, and complications after endoscopic microsurgery are rare. We report a case of sepsis and pneumoretroperitoneum after resection of a rectal lateral spreading tumor. The patient presented with rectal mucous discharge. Colonoscopy revealed a rectal lateral spreading tumor. The patient underwent an endoscopic transanal resection of the lesion. He presented with sepsis of the abdominal focus, and imaging tests revealed pneumoretroperitoneum. A new surgical intervention was performed with a loop colostomy. Despite the existence of other reports on pneumoretroperitoneum after transanal endoscopic microsurgery, what draws attention to this case is the association with sepsis.
Experimental characterization of timber framed masonry walls cyclic behaviour
Ana Maria Gonçalves,João Gomes Ferreira,Luís Guerreiro,Fernando Branco 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.2
After the large destruction of Lisbon due to the 1755 earthquake, the city had to be almost completely rebuilt. In this context, an innovative structural solution was implemented in new buildings,comprising internal timber framed walls which, together with the floors timber elements, constituted a 3-Dframing system, known as “cage”, providing resistance and deformation capacity for seismic loading. The internal timber framed masonry walls, in elevated floors, are constituted by a timber frame with vertical andhorizontal elements, braced with diagonal elements, known as Saint Andrew‟s crosses, with masonry infill. This paper describes an experimental campaign to assess the in-plane cyclic behaviour of those so called “frontal” walls. A total series of 4 tests were conducted in 4 real size walls. Two models consist of the simple timber frames without masonry infill, and the other two specimens have identical timber frames but present masonry infill. Experimental characterization of the in-plane behaviour was carried out by static cyclic shear testing with controlled displacements. The loading protocol used was the CUREE for ordinary ground motions. The hysteretic behaviour main parameters of such walls subjected to cyclic loading were computed namely the initial stiffness, ductility and energy dissipation capacity.
Quézia Manuela Gonçalves Laurindo,Túlio Almeida Peixoto,João José de Assis Rangel 한국CDE학회 2019 Journal of computational design and engineering Vol.6 No.1
This paper presents an integration mechanism for online communication between a discrete event sim-ulation (DES) software and a system dynamics (SD) software. The integration between them allowed exe-cuting a hybrid and broader simulation, in which the complexity of the systems and their multi-faceted relationships may demand the combination of different simulation methods and the synergies between the techniques. The Ururau free and open-source software (FOSS) was applied to implement the DES model. In order to build the dynamic model, we used the software for mechanical design called CAD 3D Software Inventor. Besides, we also employed the DES model in the test step of a control system in real time. The results of that mechanism implementation enabled the evaluation of different aspects of a typical manufacturing system. Furthermore, the integration between the control system and the DES model allowed validating the logic of the programmable logic controller (PLC).
Heloísa Bressan Gonçalves,João Atílio Jorge,Luis Henrique Souza Guimarães 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4
The extracellular β-D-fructofuranosidase from Fusarium graminearum was immobilized using hydrophilic cotton, filter paper, multipurpose cloth, sugar cane bagasse, string, or gauze as alternative cellulosic supports, or with cyanogen bromide agarose. All derivatives (support+enzyme) showed high capacity for reuse (up to 23 times). The derivatives obtained with multipurpose cloth and string were stable at 60℃ maintaining 80% of their activity for more than 120 min. The filter paper derivative had a halflife (T50) of 27 min at 70℃. When tested for their pH stability (3.0-9.0), all derivatives were more stable than the free enzyme, especially the cotton derivative. The sugarcane bagasse, string, and filter paper derivatives could efficiently produce fructooligosaccharides (FOS) using sucrose as substrate. According to the retention of enzymatic activity, reuse and stabilities, the filter paper and string were the best alternative supports for β-D-fructofuranosidase immobilization, allowing enzyme stabilization and production of FOS.