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Jin, Sang‐,Man,Shin, Jun Seop,Kim, Kang Seok,Gong, Chang‐,Hoon,Park, Su Kyoung,Kim, Jung‐,Sik,Yeom, Su‐,Cheong,Hwang, Eung Soo,Lee, Choon Taek,Kim, Sang‐,Joon,Park, Chung Blackwell Publishing Ltd 2011 Xenotransplantation Vol.18 No.6
<P>Jin S‐M, Shin JS, Kim KS, Gong C‐H, Park SK, Kim J‐S, Yeom S‐C, Hwang ES, Lee CT, Kim S‐J, Park C‐G. Islet isolation from adult designated pathogen‐free pigs: use of the newer bovine nervous tissue–free enzymes and a revised donor selection strategy would improve the islet graft function. Xenotransplantation 2011; 18: 369–379. © 2011 John Wiley & Sons A/S.</P><P><B>Abstract: </B><B> Background: </B> In clinical trials using adult porcine islet products, islets should be isolated from the designated pathogen‐free (DPF) pigs under the current good manufacturing practice (GMP) regulations. Our previous studies suggested that male DPF pigs are better donors than retired breeder pigs and histomorphometrical parameters of donor pancreas predict the porcine islet quality. We aimed to investigate whether the use of the newer bovine nervous tissue–free enzymes and a revised donor selection strategy could improve the islet graft function in the context of islet isolation with DPF pigs.</P><P><B>Methods: </B> Using 30 DPF pigs within a closed herd, we compared the islet yield of porcine islets isolated with Liberase PI (n =<I> </I>11, as a historical control group), Liberase MTF C/T, which is a GMP‐grade enzyme (n<I> </I>=<I> </I>12), and CIzyme collagenase MA/BP protease (n<I> </I>=<I> </I>7). We analyzed the relationship between the diabetes reversal rate of recipient NOD/SCID mice (n<I> </I>=<I> </I>75) and histomorphometric parameters of each donor pancreas as well as donor characteristics.</P><P><B>Results: </B> Proportion of islets larger than 200 μm from the biopsied donor pancreas (P<I> </I>=<I> </I>0.006) better predicted islet yield than age (P<I> </I>=<I> </I>0.760) or body weight (P<I> </I>=<I> </I>0.371) of donor. The proportion of islets larger than 200 μm from the biopsied donor pancreas was not related to the sex of the donor miniature pig (P<I> </I>=<I> </I>0.358). The islet yield obtained with the three enzymes did not differ, even after stratification of the donor with the histomorphometric parameters of the biopsied donor pancreas and the sex of donor. The use of the newer bovine nervous tissue–free enzymes (P<I> </I><<I> </I>0.001), a higher proportion of large islets in donor pancreas (P<I> </I>=<I> </I>0.006), and a male sex of the donor (P<I> = </I>0.025) were independent predictors of earlier diabetes reversal.</P><P><B>Conclusions: </B> Use of the newer bovine nervous tissue–free enzymes including a GMP‐grade enzyme resulted in better islet quality than that of islet isolated using Liberase PI. To obtain high‐quality islet from DPF pigs, the donor should be male pig and histomorphometrical parameters from donor pancreas should be considered.</P>
김진상(Jinsang Kim),이상진(Sangjin Lee),박범신(Bumshin Park) 한국자동차공학회 2009 한국자동차공학회 학술대회 및 전시회 Vol.2009 No.11
AT Shift Quality is one of major drivability target and it needs much calibration effort in the respect of cost and time. These days test work of TCU calibration increases radically due to explosion of vehicle-engine variant and complexity of control parameter. Therefore automated calibration methodology was developed for time and cost efficiency by integration of objective drivability evaluation system, vehicle driving control system and automated calibration optimization system. In addition to development time and cost saving, this new methodology has also benefits of high reliability and repeatability of test result, which were proved by demonstration test.