Radiographic Evaluation of Limb Bone Development in Miniature Porcine

Jinhwa Chang(장진화),Joohyun Jung(정주현),Mincheol Choi(최민철) 한국생명과학회 2007 생명과학회지 Vol.17 No.10

미니돼지의 긴 뼈의 뼈끝(epiphysis) 성장에 대한 연구를 위해 상완골, 요골, 척골, 대퇴골, 경골의 근위 및 원위뼈끝 부위를 중심으로 방사선 촬영을 실시하였다. 실험에 이용된 미니돼지의 연령대는 4주, 8주, 12주, 20주, 40주, 48주, 96주, 144주령으로 총 58마리였다. 뼈 성장과정에 대한 평가는 Owada & Sutow가 제안한 11가지 기준을 토대로 하였다. 긴 뼈에서 이차뼈발생중심은 4주령에서 40주령까지 빠른 성장을 보였고, 그 이후 96주령까지는 초기보다 느린 성장을 보였다. 가장 빠른 뼈끝 유합시기는 96주령으로 근위 요골, 근위 및 원위 대퇴골에서 관찰되었다. 미니돼지의 일반적인 긴 뼈의 완전한 뼈끝 유합은 144주령에 나타났으며 근위 상완골, 근위 및 원위 척골, 원위 요골, 그리고 근위 경골에서 관찰되었다. Epiphyseal development in the long bones was studied radiographically in minipigs. Radiographs of the proximal and distal epiphyses of humerus, radius, ulna, femur and tibia were obtained at 4, 8, 12, 20, 40, 48, 96 and 144 weeks of age in total 58 minipigs. The assessment of maturity process was made in accordance with the criteria proposed by Owada and Sutow. The secondary ossification centers developed rapidly from 4 weeks of age to 40 weeks of age, and gradually thereafter until 96 weeks of age. The earliest epiphyseal fusion was apparent in the proximal radius, proximal and distal femur at 96 weeks of age. The complete fusion of the epiphyseal line in the long bones was evident on 144 weeks of age and was observed in most long bones such as the proximal humerus, the proximal and distal ulna and the distal radius, and the proximal tibia in minipigs.

Evaluation of Normal Abdominal Organs by Diagnostic Imaging in the Premature Miniature Pig

Jinhwa Chang(장진화),Joohyun Jung(정주현),Sunkyoung Oh(오선경),Mincheol Choi(최민철) 한국생명과학회 2009 생명과학회지 Vol.19 No.3

4, 8, 12, 20 주령의 미성숙 미니돼지에서 방사선과 초음파를 이용하여 복부 주요 장기의 형태학적 특징을 살펴보았다. 방사선 검사는 전반적인 장기의 형태를 확인하는 데 이용되는 가장 기본적이고 단순한 방법이다. 기본 복부방사선 사진에서 이용되는 평가해야 할 항목들은 우선 전반적인 복부세부음영의 정도, 간, 위, 비장, 신장, 방광, 장들의 위치, 크기, 형태, 변연, 후복강 평가이다. 초음파 검사는 이러한 주요 장기들의 내부 실질을 평가하는 데 주로 사용된다. 미니돼지는 개와 고양이와 비교했을 때 유사한 소견을 가진 것을 확인하였다. This report describes the normal radiographic and ultrasonographic morphological features of premature minipigs at 4, 8, 12 and 20 weeks of age. Radiographic examination is a simple diagnostic method used to identify the general morphologic state of major organs and their adjacent structures, and to assess the presence of abnormalities. The parameters for evaluation in plain abdominal radiographs are the degree of diffuse serosal margin details, extent of visualization of each organ, their size, shape, and contour, locations of main organs - such as the liver, stomach, spleen, kidneys, urinary bladder - the distribution of the intestines, and assessment of the retroperitoneal space and its contents. Ultrasonographic examinations are used to investigate their internal condition. The parameters for evaluation in abdominal ultrasonography are echogenicity, echotexture, and size and shape of the main organs such as the liver, spleen, kidney, urinary bladder and gastrointestinal tract. Minipigs had similar findings compared to dogs or cats.

딴곳요관증과 요막관 잔존증 증례 보고

정주현,이관영,장진화,최민철,Jung, Joohyun,Lee, Kwanyoung,Chang, Jinhwa,Choi, Mincheol 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.3

An intact male, 3-month-old, Great Pyrenees mountain dog, weighing 7.3 kg with a history of severe hematuria during 2 months was referred. Hematology showed mild leukocytosis. There were no remarkable findings in abdominal radiographs due to ingesta and gas in the gastrointestinal tracts. Ultrasonographs showed bilateral hydronephrosis, bilateral dilated and tortuous ureters, and hyperechoic sludges in the urinary bladder (UB). And a dilated cavity was identified cranial to the UB. Excretory urography and cystography were performed to examine the cause of hydronephrosis and an abnormal cavity cranial to the UB. Bilateral ureters were tortuously dilated and opened into the craniodorsal part of the UB. An abnormal cavity was connected to the UB at the left cranioventral part of the UB. Therefore bilateral ectopic ureters and urachal remnant were diagnosed.

개에서 발생한 간 농양의 진단 영상과 치료 증례

정주현,채웅주,장진화,오선경,윤정희,최민철,Jung, Joohyun,Chae, Woongjoo,Chang, Jinhwa,Oh, Sunkyoung,Yoon, Junghee,Choi, Mincheol 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.1

A castrated male, 4-year old, Dachshund weighing 5.3 kg with an acute history of anorexia, vomiting, severe depression, and hypothermia for 1 day was referred. Severe leukopenia, mild increased hepatic enzymes, and mild imbalanced electrolyte were found on laboratory tests. There are no remarkable findings on abdominal radiographs. Ultrasonography showed multifocal heterogeneous and hypoechoic round regions in the liver. Ultrasound guided fine needle aspiration and drainage were performed and hepatic abscess was diagnosed. The patient was recovered normally without any complications through aggressive medical treatments from the result of culture and sensitivity test.

개 비강에서 발생한 악성 흑색종의 영상 진단 증례

정주현,권정국,장진화,오선경,류미현,김대용,윤정희,최민철,Jung, Joohyun,Kwon, Jungkook,Chang, Jinhwa,Oh, Sunkyoung,You, Mi-Hyeon,Kim, Dae-Yong,Yoon, Junghee,Choi, Mincheol 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.3

A neutered male, 8-year-old, Pekingese, weighing 4.3 kg with a history of anorexia, sneezing, nasal discharge, and epistaxis for one month was referred. Soft tissue swelling around the nasal bone and small defects of the hard palate with a tiny round dark red mass were found on physical examination. The laboratory tests represented mild leukocytosis. On skull radiographs, soft tissue swelling and osteolytic change of the incisor bone, nasal bone, and maxilla were found. On computed tomography scan images, there was soft tissue attenuating opacity with calcified spots in the bilateral nasal cavities and frontal sinuses. Loss of nasal turbinate pattern and nasal septum was found. And destruction of the insicor bone, nasal bone, maxilla, hard palate, perpendicular palatine bone, and cribriform plate were identified. Nasal malignant melanoma was confirmed by nasal biopsy.

개에서 자궁축농증과 병발한 척추원반염 1례

김학상,최지혜,정주현,장진화,최민철,윤정희,Kim, Haksang,Choi, Jihye,Jung, Joohyun,Chang, Jinhwa,Choi, Mincheol,Yoon, Junghee 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.4

Discospondylitis is an infection of intervertebral discs, associated end plates, and adjacent vertebral bodies causing destruction and proliferation. A 10-year-old intact female Rhodesian Ridgeback dog was referred to the Veterinary Medical Teaching Hospital, Seoul National University with paraparesis and severe pain at T13-L1 region for one week duration. The treatment with PDS for one week by referring veterinarian showed no clinical improvement. At time of presentation, clinical signs of the patient included depression, vaginal discharge, and fever. In T-L spine radiography, osteolytic change was found between caudal endplate of T13 and cranial endplate of L1. The affected vertebral bodies were shortened and intervertebral disc space was widened. In abdominal radiography, a dilated soft tissue opacity tubular structure was found in mid-caudal abdomen. The abdominal ultrasonography revealed the uterus dilated with echogenic fluid. The CT findings showed concentric lysis of T13-L1 endplates. The dog was diagnosed as diskospondylitis at T13-L1 and pyometra. Urine and blood were cultured, and Staphylococcus (S.) intermedius was identified. The patient had surgical treatment for pyometra but died for septicemia.

Hematology and Serum Chemistry Values of Growing Miniature Pigs

Joohyun Jung(정주현),Jinhwa Chang(장진화),Haksang Kim(김학상),Junghee Yoon(윤정희),Mincheol Choi(최민철) 한국생명과학회 2008 생명과학회지 Vol.18 No.8

본 실험의 목표는 미니돼지에서 성성숙 전까지의 성장단계에서 혈액성분을 조사하는 것이다. 12마리 돼지를 이용하여 정맥에서 채혈하였다. 전혈과 혈청을 사용하여 혈액 및 혈액 화학치를 분석하였다. 그리고 성장단계별의 조사에서 각 성분은 평균과 표준편차로 나타내었다. 혈액분석에서 적혈구와 적혈구용적 그리고 혈소판에서 유의치 있는 변화가 있었다. 특히 적혈구와 적혈구용적은 4주령 군에서 가장 낮은 수치를, 반면에 혈소판은 4주령에서 가장 높은 수치를 보였다. 혈액 화학치에서는 glucose, GGT에서 유의치 있는 변화가 있었으며, 나머지 성분에서는 유의치 있는 변화가 없었다. 이러한 기본적인 혈액 및 혈액 화학치는 미니돼지의 정상 혈액학 연구와 그리고 성장단계별 이른 시기에 정상돼지를 판별하는데 유용하리라 생각된다. The purpose of this study was to establish blood reference values for miniature pigs from various growth ages before pre-puberty. 12 miniature pigs from two swine farms were sampled by jugular venipuncture. Routine hematological and biochemical determinations were performed using whole blood and serum. For the variables examined in each age group the means and the standard deviations were calculated. In hematological values, there are significant changes (p<0.05) in RBC, hematocrit and platelet values in each group. In RBC and hematocrit values, 4-week group shows the lowest level, whereas in platelet values, 4-week group shows the highest level. In biochemical values, there are significant chnges (p<0.05) in glucose and GGT in each group, and in other biochemical values there are no significant changes during growing periods. The values presented here may be useful for knowing the normal values for miniature pigs and also screening the normal pigs in the early days of their life and during the developemental stages.