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이진석(Jin-Seok Lee), 서형석(Hyeong-Seok Seo), 김소정(So-Jung Kim), 김형진(Hyeong-Jin Kim), 김진(Jin Kim), 이성호(Seung-Ho Lee), 박영석(Young-Seok Park), 박병권(Byung-Kwon Park), 김병수(Byeong-Soo Kim), 김상기(Sang-Ki Kim), 정지윤(Ji-Youn Jun) 한국생명과학회 2010 생명과학회지 Vol.20 No.10
Apigenin은 과일과 야채에 들어있는 플라보노이드로 다양한 악성 세포에 항증식효과를 보여준다. 세포성장 저해효과를 확인하기 위하여 KB 구강암세포주를 96 well plate에 6×10³ cells/well로 분주하고 24시간 후에 apigenin을 24시간 동안 처치하여 MTT assay를 수행하였다. Apigenin은 배양 후 용량 의존적으로 세포사를 유도하였다. Apigenin 100 μM을 24시간 동안 처치하고 대조군과 세포성장을 비교하였을 때 유의적인 감소를 확인하였다. KB 구강암세포주에서의 apoptosis를 확인하기 위해 DAPI 염색을 수행하였다. Apigenin을 처치한 세포에서 핵의 응축이 존재함을 형광현미경으로 확인하였다. 우리는 누드마우스에 KB 구강암세포주를 이식하여 세포 성장 억제 효과를 알아보았다. Apigenin을 마우스에 25, 50 ㎎/㎏을 0.2 ml의 PBS에 녹여 경구투여 하였다. 종양 사이즈는 대조군과 25, 50 ㎎/㎏ apigenin 투여군을 비교하였을 때 유의적으로 감소하였다. Apoptosis 분석을 위해 TUNEL염색을 수행하였다. 25 ㎎/㎏ apigenin 투여군과 대조군을 비교하였을 때 apoptosis의 유의적인 증가를 확인하였다. 육안적 소견을 위한 H&E 염색은 이상이 없었다. 본 연구는 apigenin이 구강암세포주 성장 억제를 apoptosis의 유도를 통하여 확인하였다. Apigenin (4', 5, 7-trihydroxyflavone), a common dietary flavonoid abundantly present in fruits and vegetables, has shown remarkable anti-proliferative effects against various malignant cell lines. To observe the anti-proliferative effects, oral cavity cancer cell lines, 6×10³ cells/well (96 well plate) of KB oral cavity tumor cells were plated and 24 hr later treated with apigenin for one day, after which MTT assay was performed. Apigenin induced cell death in a dose-dependent manner after incubation. Cell viability was significantly decreased in the group treated with 100 μM apigenin for 24 hr (p<0.05) compared to the control group. To assess apoptosis, the nuclei of KB cells were stained with DAPI. The presence of chromatin condensation in the apigenin treated cells was detected on a fluorescent microscope (×200). We investigated the in vivo growth inhibitory effects of apigenin on oral cavity cancer KB tumor xenograft subcutaneously implanted in male nude mice. Apigenin was administered to mice by gavage at doses of 25 and 50 ㎎/㎏/day in 0.2㎖ of PBS. Tumor volume was significantly decreased in 25 and 50 ㎎/㎏ apigenin-administration groups compared to the control group. For apoptosis analysis, TUNEL staining was performed. A significant increase in TUNEL positive cells was found in the 25 ㎎/㎏ apigenin administration group compared to the non- apigenin administration group. Histopathological changes were not observed. These results indicate that apigenin inhibits oral cavity cancer cell growth through the induction of apoptosis.
To understand the oviposition biology of arrowhead scale, Unaspis yanonensis (Kuwana) (Homoptera: Diaspididae), the longevity and fecundity of the overwintered females were examined at various temperatures (16, 20, 24, 28, and 32℃). The total fecundity increased with rising temperature, showed a maximum of 260.9 crawlers per female at 24℃, and then declined thereafter. Females showed a periodical oviposition activity. The 1st cycle fecundity was identified at all temperatures examined, and was not significantly different between 16 and 28℃. However, the 2nd cycle fecundity and later cycle fecundity were much lower at 16, 20, and 32℃ than those at 24 and 28℃. The pre-oviposition period ranged from 49.0 d at 16℃ to 19.7 d at 32℃, and was the shortest 19.4d at 28℃. The linear lines of mean development rates (1/mean pre-oviposition period) against temperatures provided the estimate of low-threshold temperature 8.7℃ for pre-oviposition stage and the thermal constant of 358.1 DD. The lower threshold temperature and thermal constant for the completion of U. yanonensis pre-oviposition period well predicted the first crawler occurrences in the fields.
Male pseudohermaphroditism is not commonly reported in veterinary medicine. Here, a 3-year-old Maltese/poodle mixed dog presented with malformed external genitalia and episodic hematuria. Inspection and palpation of the external genitals showed a malformed penis, shortened prepuce, external urethral orifice, and cryptorchidism. There was no urethral meatus at the tip of the penis. The urethral opening was situated between the prepuce and the penis. The anterior half of the prepuce was absent, and the penis was free and exposed to both trauma and licking. Plain radiographic examination showed absence of an os penis in the penis. A double-contrast cystograph showed the suspected uterus as well as the cystic calculi. A hypoechoic space was seen at the dorsal portion of the urinary bladder. The space was suspected to be the uterus. A sagital ultrasonograph showed cystic calculi in the urinary bladder. During surgery to remove cystic calculi, hypoplastic testes as well as the uterus were observed. Histological examination of the testes showed the seminiferous tubules and interstitial cells. The sertoli cells and spermatogonia were adjacent to the basement membrane. No evidence of spermatogenesis was found. Striated squamous epithelial cells and smooth muscle cells were found in the uterus. This dog had vestigial oviducts as well as a uterus with male-appearing external genitals.
The present paper deals with the continuous work of extending multi-dimensional limiting process (MLP) for high speed compressible flows (or hyperbolic conservation laws), which has been quite successful in finite volume method (FVM), into discontinuous Galerkin (DG) methods. Based on successful analyses and implementations of the MLP slope limiting in FVM, MLP is applicable into DG framework with the MLP-based troubled-cell marker and the MLP slope limiter. Through several test cases, it is observed that the newly developed DG-MLP methods provide quite desirable performances in controlling numerical oscillations as well as capturing key flow features.
Background/Aims: Common bile duct stones are associated with the extent of dilation of the common bile duct as well as its angulation. Multidetector computed tomography (MD CT) has a good resolution for the definition of the anatomical features of the common bile duct. Methods: The multiplanar reformation images of 398 patients that underwent multidetector CT for the diagnosis of disorders not related to the bile duct were examined. The diameter and angulation were categorized by gender and age. Results: The average diameter and angulation of the common bile duct was 6.7 mm and 132.6°. There was a statistically significant correlation between age and the common bile duct diameter. The Pearson correlation analysis for age and diameter resulted in a value of 0.415 (p<0.001). And the common bile duct (CBD) diameter in people older than 51 years of age showed a significant difference compared to the subjects younger than 50 years of age (p<0.01). However, the degree of angulation has no correlation with age. Conclusions: We suggest that CBD diameters in patients more than 50 years of age can be more than 7 mm and be within normal limits. (Gut and Liver 2009;3: 306-310)
With the advent of flash memory based new storage device (SSD), there is considerable interest within the computer industry in using flash memory based storage devices for many different types of application. The dynamic index structure of large text collections has been a primary issue in the Information Retrieval Applications among them. Previous studies have proven the three approaches to be effective: In- Place, merge-based index structure and a combination of both. The above-mentioned strategies have been researched with the traditional storage device (HDD) which has a constraint on how keep the contiguity of dynamic data. However, in case of the new storage device, we don' have any constraint contiguity problems due to its low access latency time. But, although the new storage device has superiority such as low access latency and improved I/O throughput speeds, it is still not well suited for traditional dynamic index structures because of the poor random write throughput in practical systems. Therefore, using the experimental performance evaluation of various index maintenance schemes on the new storage device, we propose an efficient index structure for new storage device that improves significantly the index maintenance speed without degradation of query performance.
This paper introduces the design method for the practical use of a wave energy converter (WEC), and the associated results are application to the commercially available WEC for buoys. Peak performance of WEC occurs at resonance with driving waves. This type of resonance occurs when one of the parameters in an oscillator varies periodically. The water column in a WEC oscillates under the effect of gravity and the compression of an air chamber. The analysis of WEC is developed by assuming independence of the buoy heaving motion and the motion of the water column within the center cylinder. Results of analysis are then compared with simulation data, and applied to designing a WEC for buoys. Also, the effect of the various parameters such as cylinder length, period, mass and wave height is analyzed for the optimum design of a WEC. Finally, the research results are applied to a wave simulator with operating LabView, and some ideas are presented to the design method of WEC for buoy with simulation experiment.
We propose an global color model based method for tracking motions of multiple human using a networked multiple-camera system in intelligent space as a human-robot coexistent system. An intelligent space is a space where many intelligent devices, such as computers and sensors(color CCD cameras for example), are distributed. Human beings can be a part of intelligent space as well. One of the main goals of intelligent space is to assist humans and to do different services for them. In order to be capable of doing that, intelligent space must be able to do different human related tasks. One of them is to identify and track multiple objects seamlessly. In the environment where many camera modules are distributed on network, it is important to identify object in order to track it, because different cameras may be needed as object moves throughout the space and intelligent space should determine the appropriate one. This paper describes appearance based unknown object tracking with the distributed vision system in intelligent space. First, we discuss how object color information is obtained and how the color appearance based model is constructed from this data. Then, we discuss the global color model based on the local color information. The process of learning within global model and the experimental results are also presented.
Localization of mobile agent within a sensing network is a fundamental requirement for many applications, using networked navigating systems such as the sonar-sensing system or the visual-sensing system. To fully utilize the strengths of both the sonar and visual sensing systems, This paper describes a networked sensor-based navigation method in an indoor environment for an autonomous mobile robot which can navigate and avoid obstacle. In this method, the self-localization of the robot is done with a model-based vision system using networked sensors, and nonstop navigation is realized by a Kalman filter-based STSF(Space and Time Sensor Fusion) method. Stationary obstacles and moving obstacles are avoided with networked sensor data such as CCD camera and sonar ring. We will report on experiments in a hallway using the Pioneer-DX robot. In addition to that, the localization has inevitable uncertainties in the features and in the robot position estimation. Kalman filter scheme is used for the estimation of the mobile robot localization. And Extensive experiments with a robot and a sensor network confirm the validity of the approach.