Inhibition of oxidative stress induced‑cytotoxicity by coptisine in V79‑4 Chinese hamster lung fibroblasts through the induction of Nrf‑2 mediated HO‑1 expression

Hyeon‑Gyun Jo,Cheol Park,이혜숙,Gi‑Young Kim,Young‑Sam Keum,Jin Won Hyun,Taeg Kyu Kwon,Yung Hyun Choi,Su Hyun Hong 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background Coptisine is a natural alkaloid compound and is known to have multiple benefcial efects including antioxidant activity. However, whether it can protect lung fbroblasts from oxidative damage has not been studied yet. Objectives To investigate the potential inhibitory efect of coptisine against oxidative stress in V79-4 lung fbroblast cells. Methods V79-4 cells were treated with H2O2 (1 mM) in the presence or absence of coptisine (50 µg/ml), N-acetyl cysteine (NAC, 10 mM) or zinc protoporphyrin IX (ZnPP, 10 µM) for the indicated times. The alleviating efects of coptisine on cytotoxicity, cell cycle arrest, apoptosis, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of ATP production against H2O2 were investigated. Western blot analysis was used to analyze the expression levels of specifc proteins. Results Coptisine inhibited H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS generation. H2O2 treatment caused cell cycle arrest at the G2/M phase accompanied by increased expression of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 and decreased expression of cyclin B1 and cyclin A. However, these efects were attenuated in the presence of coptisine or NAC. Coptisine also prevented apoptosis by decreasing the rate of Bax/Bcl-2 expression in H2O2-stimulated cells and suppressing the loss of mitochondrial membrane potential and the cytosolic release of cytochrome c. In addition, the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was markedly promoted by coptisine in the presence of H2O2. However, zinc protoporphyrin IX, a potent inhibitor of HO-1, attenuated the ROS scavenging and anti-apoptotic efects of coptisine. Conclusions Based on current data, we suggest that coptisine can be used as a potential treatment for oxidative stress-related lung disease.

진주지역 제조기업의 산업재해 실태분석과 대책방안

최진현,강호욱 경상대학교 경남문화연구소 1983 慶南文化硏究 Vol.6 No.1

The process of manufacturing enterprise takes the potential injury agents (moving equipment, machines and tools, hazards, etc). The industrial accidents due to these occasion happen unexpectedly. Safety management, in the manufacturing enterprise, are a moral action for worker, and improve the production efficiency and decrease the accident cost. In this study, industrial accidents are analyzed on the actual condition for 339 manufacturing enterprise, and select the 9 sampling enterprise. We simulate the relationship between accident cost and safety management cost. So, We suggest the safety management counterplan. The actual condition of the industrial accidents were analyzed by I.L.O. statistical analysis equations, which are expressed by (1) and (2). FR=N/H×10^6 …(1) SR=S/H×10³ …(2) Logical development of the alternatives for safety management counterplan based on the structure of industrial accident. We derived characteritics of the industrial accidents, and suggested a few counterplan of industrial accidents for manafacturing enterprises in Jin-Ju area.

휴대용 핵종 분석기의 유용성 평가

김지현(Ji Hyeon Kim),전성진(Seong Jin Jin),제재용(Jae Yong Je) 한국방사선학회 2015 한국방사선학회 학술대회 논문집 Vol.2015 No.춘계

본 연구는 휴대용 핵종분석기를 이용하여 부산지역의 지역별 환경방사선을 측정하여 휴개용 핵종분석기의 유용성을 평가하고자 한다. 측정 대상은 부산의 중심에 위치한 부산진구 소재 송상현 광장을 중심으로 9곳의 환경 방사선을 측정하였다. 측정결과 부산역과 부산대학교를 제외한 대부분의 지역에서 환경방사선은 비슷한 결과를 나탄내었다. 검출된 핵종은241Am, 133Ba, 54Mn등이 검출되었고 핵종분석기에 내장된 핵종정보가 부족하여 알 수 없는 핵종들도 검출되었다. 실험에 사용한 휴대용 핵종분석기는 분해능이 8% 정도로 자연방사성 핵종과 인공방사성 핵종을 핵종별로 정확하게 분석하기 위해서는 제조사의 개선이 필요한 것으로 판단된다. This research aims at measuring the environmental radioactivity at each area of Busan using a potable radio-isotope identification system, and assessing the utility of the potable radio-iosotope identification system. As for the target for measurement, the environmental radioactivities of nine areas centered around Songsanghyeon Square in Jin-gu located in the center of Busan were measured. As the measurement result, the environmental radioactivities were similar in most of the areas except in Busan station and Busan University. The nuclides, such as 241Am, 133Ba, 54Mn and so on, were detected, and some nuclides whose identity was unknown because of lack of information built in the radiation spectrum analyzer were also found. The potable radio-isotope identification system used in the experiment had about 8% of resolving power, so for more precise analysis into the nuclides of natural background radiation and artificial radiation in each type, the manufacturer needs to improve the analyzer.

올레산의 비율에 따른 아데노신의 경피 투과의 영향

남현빈,정세진,구민석,오예진,김용민,이훈희,윤수정,임정균 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

In addition to the development of functional cosmetics, technology to increase percutaneous absorption rate when applied to the skin is an important task. In this experiment, oleic acid was used as a skin absorption promoter, and its effect on transdermal permeability of adenosine was studied and analyzed using a skin-like membrane. As a result of the experiment, it was investigated that the permeability coefficient and concentration of adenosine are proportional to the content of oleic acid. This indicates that a high proportion of oleic acid is effective as a skin absorption promoter.

진행성 폐 종양 환자에서 종양 내 OK-432 주입술의 효과

정수현,김성빈,박진희,탁희상,옥철호,장태원,정만홍,천봉권 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background: Intratumoral injection of OK-432 produced significant antitumor effects. There are reports that OK-432 was injected directly into tumor tissue on gastrointestinal tracts and HCC tissue, producing decrease in serum alpha-fetoprotein level. We adopted this technique for treating locally advanced lung cancer. The object of this study is to evaluate the local therapeutic efficacy, side effects of injection for treating symptomatic mass. Methods: Twenty lung cancer patients (13 males and 7 females) underwent sonography guided OK-432 injection. We analyzed relationships between response of OK-432 and response of skin test, histology and age. Pain that patients feel was evaluated by VAS (Visual Analog scale). Results: A significant decrease of tumor size was observed. The mean pre-injection diameter of the lesions was 54.7±11.3mm, PR rate were 15.0% (3/20) and minimal change (50%<tumor size decreased.) patients were 35.0% (7/20). The VAS score before injecting OK-432 into the lesion was 5±3, this was decreased to 2.5±1.5 (p=0.04). Object response was significantly correlated with skin response, adenocarcinoma, and age. Conclusion: A significant decrease of tumor size and pain was observed. So OK-432 is a valuable treatment option for patients with symptomatic lesion from advanced lung cancer who are not amenable to further therapy.

WIPI 기반 모바일 3D 게임엔진의 설계와 구현

형대진,김승구,조현준,박경환 동아대학교 정보기술연구소 2006 情報技術硏究所論文誌 Vol.14 No.1

In this paper, we introduce the method for developing the mobile 3D game engine based on the WIPI platform which has been proposed as the mobile standard platform. Our game engine is designed so that it supports OpenGL-ES and JSR-184 that are mobile 3D graphic APIs. Our game engine has a mobile emulator operated on PC and SDK to support mobile programming in PC environments. Mobile 3D game developers can use the APIs at various levels. Also, the game engine is developed in modular in order to support various game genres.

청소년 지도학의 활동영역 활성화 연구

육현철,장갑석,최강진 한국체육대학교 2001 韓國體育大學校 敎養敎育論文集 Vol.- No.6

The Curriculum of National Cooking(Guidance) for the Youth Had Been established at the Myungji University in 1994 for the first time in the history of Korean college, and 8 universities Has, Recently and operate the course, These days the curriculum mostly concentrates on the subject of pedagogy and educational psychology. (ⅰ) To vitalize the juvenile's physical activity, the government concerned needs to make a political support in order each university to found the curriculum for the youth.And each university must open the course which the teenager can make physical activities free of charge. (ⅱ) The educational policy researching and developing the activity area of the youth is required.By educating them based upon the activity area, the youth can have a healthy body which results from sound spirit. (ⅲ) The youth Activity Centre is to be established and operated at each community or local district to vitalize the section for sport activity which the juvenile prefer.Physical promotion is needed to be desirable adult. (ⅳ) Today the computer system like internet and dlectronic entertainment/game give the large influence on all the juvenile.Herein we need to remind that almost young generation study by day and indulge in computer without Physical activity that lead to weak body and feeble spirit.Under those circumstances, we can find out the juvenile delinquent from to time. (ⅴ) Finally, it is greatly important and imperative to foster lots of professional experts/coaches who can understand the youth and guide them in a sound way.

B. Malamud의「The Assistant」에 나타난 Jewishness에 대한 고찰

韓玄眞 尙志大學校 1981 論文集 Vol.2 No.-

폴리에틸렌 코팅 가스배관의 광범위탐상을 위한 유도초음파 모드 선정 및 결함 검출

송성진,박준수,신현재 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.4

폴리에틸렌 코팅 가스배관의 결함탐상을 위해 광범위 비파괴탐상기법인 유도초음파를 적용하였다. 가스배관의 내경은 190.7mm, 두께는 5.3mm, 그리고 바깥 표면은 1.9±0.5mm의 폴리에틸렌 층으로 코팅되어 있다. 폴리에틸렌 코팅 배관의 외면에 0.5MHz 탐촉자와 가변각 웨지를 사용하여, 비축대성 유도초음파를 가진 시겼다. 주파수와 위상속도 조정을 통하여 가스배관의 결함 탐상을 위한 적절한 모드를 찾아내고, 모드의 특성을 단시간 퓨리어 변환을 이용하여 분석하였다. 결함탐상 결과를 두께 손실 및 원통형 관통결함에 대한 인위결함에 대해 나타내었다. 그리고 가스배관에 인위적으로 삽입한 두께 손실과 원통형 관통결함에 대한 검출 성능을 평가하였다. Ultrasonic guided waves were explored to apply them to the long range inspection of polyethylene coated steel gas pipes. The steel pipes have such dimensions as 190.7mm inside diameter and 5.3mm thickness. The outside surface of the pipe is coated by a polyethylene layer of 1.9±0.5mm thickness. Non-axisymmetric guided waves were excited on the outside surface of the polyethylene coated pipe by using a 0.5 MHz transducer with a variable angle shoe. Frequency and phase velocity tuning was used to find optimum guided wave modes for the inspection. The dispersive characteristics of the modes were analyzed in time-frequency representation obtained by short time Fourier transforms. Sample results were presented for artificial defects such as wall thinning and hole.

센서 노드의 프로그래밍 환경 비교

윤현주,오병진 금오공과대학교 2006 論文集 Vol.27 No.-

Software development environment is one of important features to extend the application area and to make rapid progress in any computing application fields. We studied and compared the programming environments for wireless sensor networks, which becomes an essential technology towards the ubiquitous era. Two representative environments, TinyOS/nesC and Nano Qplus/C were investigated and the comparison was performed by implementing a typical application including sensing and multi - hop transmission. We experienced and observed that the TinyOS/nesC environment is difficult at initial stage due to the unfamiliar programming model, but the result codes are more compact and have abundant reference implementations. On the other side, Nano Qplus/C environment is easy to adaptable for general purpose programmers and provides more features such as multi-threading and dynamic memory allocation.