한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

폐굴껍질의 정석반응을 이용한 수중 인제거 특성

이호수,정유진,김은호,성낙창,이영형 동아대학교 환경문제연구소 1998 硏究報告 Vol.21 No.2

The technology of removing phosphorous, considered as one of the most important control nutrients causing eutrophication in various water bodies, have been investigated by many researchers. Recently, phosphorous crystallization process is emerging as a new technology for phosphorous removal. In this study, waste oyster shells which can be easily obtained from the ocean, were used as a seed crystal, and their effects of several physical/chemical factors on the phosphorous removal efficiencies were examined by batch tests. Ca^(2+) and pH affected phosphorous crystallization process using waste oyster shells. As alkalinity of wastewater increased, phosphorous removal efficiencies gradually decreased. Phosphorous removal efficiencies were increased, as specific area and contact efficiency per unit area of waste oyster shells were increased. In case of high temperature, phosphorous crystallization process was rapidly advanced and phosphorous removal efficiencies were increased. Depending on X-ray diffraction analysis, it was showed that generation materials extracted from the surface of waste oyster shells with short reaction time were dominated by CaHPO₄ ·2H₂O, but progressed to Ca_(5)(OH)(PO₄)₃.

연 폭로가 남성호르몬에 미치는 영향

김형수,장성훈,이원진,최재욱,박종태,백남원,김록호 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.1

목 적 : 본 연구는 직업성 연 폭로근로자에서 연폭로가 남성 생식기계 독성중 성호르몬에 미치는 영향과 그 정도를 평가하고자 시도되었다.방 법 1998년 하반기에 실시한 일반, 특수건강진단자중 경기도 지역 직업성 연 폭로 근로자 33명과 과거 직업력에서 연 폭로가 없었던 사무직 근로자 33명을 연구대상으로 선정하였다. 연의 생물학적지표로는 혈색소, ZPP, 혈중 연, 및 요중 연을 이용하였으며, 성호르몬으로는 LH, FSH, testos-terone, SHBG 및 FTI를 이용하였다. 연 폭로 지표와 성호르몬과의 상관관계를 조사하였다. 결 과 : 연의 생물학적 지표중 혈색소는 폭로군에서 대조군보다 유의하게 낮았으며, ZPP, 혈중 연및 요중 연등은 폭로군에서 대조군보다 유의하게 높았다. 성호르몬에서 FSH, testosterone, SHBG및 FTI는 모두 폭로군에서 대조군보다 낮았으나 통계적 유의성은 없었다. 폭로군의 성호르몬중에서 연령은 FSH 및 FTI, ZPP는 FSH, 혈중 연은testosterone과 유의한 상관관계가 있었다. 대조군에서 연령은 FSH, testosterone, SHBG 및 FTI와 흡연량은 FSH와 유의한 상관관계가 있었다. 연령군별 폭로군과 대조군의 성호르몬 비교에서 30-39세군의 FTI에서 폭로군이 대조군보다 유의하게 낮았으며 그 밖의 호르몬에서는 모두 유의한 차이가 없었다. 결 론 : 연 폭로군의 FSH, Testosterone, SHBG 및 FTI가 대조군에 비교하여 낮았으나 통계적 유의성은 없었다. 그러나 폭로군에서 연의 생물학적 지표와 일부 성호르몬간에 유의한 상관관계를보였으며 이는 본 연구 대상 근로자의 연 폭로 수준이 일부 성호르몬에 영향이 있음을 보여주는 것으로 판단된다. 향후 이에 대한 지속적인 연구가 진행되어 야 할 것이다. Objectives : This study was performed to evaluate the relationship between biological markers of lead exposure and level of sex. hormones in men occupationally exposed to lead. Methods : In this study, 33 male workers who employed at smelting and battery factories were compared with 33 male workers who were not exposed to lead. As biological markers of lead exposure, hemoglobin, ZPP, blood lead and urine lead were measured. As sex hormones, FSH, LH, testosterone, SHBG and FTI were determined. Results : Hemoglobin level was significantly lower in exposed than in nonexposed group. Level of ZPP, blood lead, and urine lead were significantly higher in exposed than in nonexposed group. There was a trend that level of FSH, testosterone, SHBG, and FTI was lower in exposed than in nonexposed group respectively, but there were no statistical significance. In exposed group, FSH level was correlated with age, ZPP, and blood lead; testosterone level was correlated with hemoglobin, blood lead, urine lead, and SHBG ; and FTI level was correlated with age. Conclusions : Level of some sex hormones were lower in exposed than in nonexposed group but there were no statistical significance between the exposed and the nonexposed group. But level of sex hormones were correlated with several biological markers of lead in exposed group. It suggests that occupational lead exposure might affect sex hormones.

남부 평야지 자운영재배답에서 논유형과 질소수준이 벼 생육 및 토양 화학성에 미치는 영향

윤봉기,김병호,이인,최형국,박인진,김용웅 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.3

녹비작물로서 자운영을 재배한 논에 벼를 재배할 때 질소비료의 절감기준을 구명하고자 평야지인 전남 나주의 보통답과 사질답에서 관행, 자운영+질소 70%, 자운영+질소 50%, 자운영+질소 30%, 자운영+무질소의 5처리로 1999년부터 2001년까지 3년동안 시험한 결과는 다음과 같다. 1. 자운영의 개화성기 생육은 초장 43.6~43.9㎝, 생초중 21.79~24.99Mg ha^(-1)이였으며 무기성분 함량은 T-N 2.40~2.64%, P₂O_(5) 0.42~0.46%, K₂O 1.83~2.01%였다. 자운영의 생육은 보통답이 사질답에 비하여 생초중이 많았고 T-N, T-C, P₂O_(5), CaO, MgO의 함량도 높았다. 2. 토양중 NH₄-N함량의 경시적 변화는 이앙후 7일에 20.7~47.4㎎ kg^(-1)으로 점차 감소하였는데 처리에 따라서는 자운영+질소 70%, 자운영+질소 50% 시용구에서 높았다. 3. 벼의 생육은 사질답이 보통답에 비하여 초장 및 간장이 크고 ㎡당경수가 많았고 처리에 따라서는 자운영+질소 50%, 자운영+질소 30%시용구에서 생육이 좋았다. 식물체중의 T-N, P₂O_(5), K₂O함량도 자운영+질소 70%, 자운영+질소 50%시용구에서 많은 편이었다. 4. 시험후 토양의 화학성은 관행에 비하여 보통답의 자운영 시용구에서 OM, CEC, Ava, P₂O_(5)함량이 증가하였고 사질답에서는 CEC가 증가하였다. 5. 벼의 수량은 관행(보통답 5.15Mg ha^(-1), 사질답 5.16Mg ha^(-1))에 비하여 보통답에서는 자운영+질소 70%, 50%, 30%시용구에서 각각 3, 6, 4% 증수되었고, 사질답에서는 자운영+질소 70%, 50%시용구에서 각각 3, 5%증수되었다. 6. 남부 평야지에서 자운영을 재배하여 생초 20Mg ha^(-1)를 녹비로 투입하면 벼를 재배시 질수시용 적정량을 보통답에서는 관행시비량의 30%, 사질답에서는 관행시비량의 50%이였다. This study was conducted to find out an amount of N fertilizer for rice culture followed after milk vetch(MV) cultivated at normal and sandy paddy field in Naju which lies to southern plain region from 1999 to 2001. The amounts of N fertilizer were 110(normal paddy) and 130(sandy paddy)㎏ ha^(-1) at control and 0, 30, 50, 70% of control. At flowering stage of MV, Plant height was 43.6~43.9㎝ and fresh weight was more than that of sandy paddy field. Also, the contents of T-N, T-C, P₂O_(5), CaO, MgO in MV plant cultivated normal paddy field were higher than those of sandy paddy field. Content of NH₄-N in soil was 20.7~47.4㎎ ㎏^(-1) at 7days after transplanting and 6.1~8.1㎎ ㎏^(-1) at 70days after transplanting of rice plant. Content of NH₄-N in soil was high at the treatments of MV+N50% and MV+N70%. Rice plant height, culm length and the number of tiller per ㎡ in sandy paddy field were higher than those of normal paddy field. Growth of rice plant in MV+N70% and MV+N50% was higher than that in others. And the content of T-N, P₂O_(5) and K₂O of rice plant in MV+N70% and MV+N50% were higher than those in others. Chemical properties of soil after experiment were pH 5.1~5.9, OM 15.6~20.9g ㎏^(-1). CEC 10.1~13.8cmol ㎏^(-1) and Ava. P₂O_(5) 152~244㎎ ㎏^(-1). The contents of OM. CEC and Ava. P₂O_(5) in soil applied MV at normal paddy field were higher than those of the control, and the content of CEC in soil applied MV at flowering stage, rice yields of MV+N70%, 50%, MV+N30% were increased 3, 6, 4% more than that of control (5.15Mg ha^(-1)) at normal paddy field. Rice yields of MV+N70%, MV+N50% were increased 3, 5% higher than that of control(5.16Mg ha^(-1)) at sandy paddy field. Nitrogen application rate of normal paddy field was 30%, and sandy paddy field was 50% of conventional fertilization rate when 20Mg ha^(-1) MV cultivated paddy field was inputed for rice culture in the southern plains.

C3H/Hej 마우스에서 모발성장에 대한 생약 추출물의 효과

구성근, 주진호, 허진주, 미가, 강봉수, 김준형, 남상윤, 윤영원, 이범준, 김종수 충북대학교 동물의학연구소 2008 Journal of Biomedical and Translational Research Vol.9 No.3

본문참고

정신분열병과 Neurotensin 수용체 유전자 다형성의 연합 연구

이유상,김형배,한진희,채영규,이정식,이혜순,주연호,김형섭,최인근,양병환 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6

연구목적: Neurotensin(NT)은 NT수용체와 결합하여 그 효과를 나타내는 neuromodulator 혹은 neurotransmitter로서 대뇌에서 도파민의 분비를 조절하는데 중요한 역할을 한다. 근래의 연구에 의하면 NT와 그 수용체는 대뇌에서 항정신병 약물의 효과를 매개하는 것으로 생각되고 있으며 약물치료를 받지 않은 정신분열병 환자의 뇌척수액에서 NT의 양이 적으로 보고되고 있어 이들은 정신분열병과 깊은 관련을 가지고 있을 것으로 추정된다. 최근 NT수용체의 유전자의 3`인접영역에서 정보가치가 높은 4 염기반복 다형성이 발견되어 이를 유전 표지자로 하여 정신분열병과의 연합을 알아보았다. 방 법: 서로 혈연관계에 있지 않은 정신분열병 환자 120명(남자 91명, 여자 29명)과 정상 대조군 106명(남자 84명, 여자 22명)을 대상으로 하였다. PANSS를 사용하여 양성 및 음성을 알아보았다. 말초혈액에서 DNA를 분리한 후에 중합효소연쇄반응을 사용하여 3`인접영역에 있는 4 염기 반복 다형성을 증폭하였고 silver staining한 후에 유전자형을 관찰하였다. chi-square 검증과 Bonferroni`s correction을 사용하여 환자군과 정상 대조군간의 대립유전자 빈도의 차이를 알아보았다. 또한 양성 및 음성 환자군으로 나누어 차이를 알아보았다. 결 과: 총 23개의 대립유전자가 관찰되었으며, 399bp의 대립유전자(A10)의 빈도가 환자군보다 정상대조군에서 통계적으로 유의하게 높았다(χ²=16.49, df=1, p<0.001). 음성 정신분열병 환자군과 정상대조군 사이의 비교에서는 정상대조군의 A10의 빈도가 환자군보다 유의하게 높았다(χ²=21.33, df=1, p<0.001). 성별 비교에서 남자 정신분열병 환자군은 대조군에 비하여 A10의 분포가 유의하게 적었다. (χ²=13.71, df=1, p<0.001) 결 론: NT 수용체 유전자와 정신분열병사이에 음성연합이 관찰되었다. NT 수용체 유전자가 일부 정신분열병의 발병과정에서 확실하지는 않지만 어떤 종류의 보호기능을 할 수도 있다는 것을 암시한다. Objectives: Neurotensin(NT), of which functions are evoked by its interaction with neurotensin receptors(NTR), coexists with mesolimbic dopamine and regulates endogenous dopamine release. Recent studies have shown that NT with NTR exerts neuroleptic-like activity within the central nervous system and may play an important role in the pathogenesis and in the treatment of schizophrenia. We have examined the gentic association between schizophrenia and tetranucleotide repeat polymorphism in the 3-flanking region of the NTR gene to investigate the possible contribution of the NTR gene to the schizophrenia susceptibility. Methods: Among 23 alleles identified, the subjects were 120 patients(male 91, female 29)with schizophrenia and 106 normal healthy controls(male 84, female 22). They were unrelated native Korean. PANSS was used to determine positive or negative subgroup in the schizophrenic patients. Using polymerase chain reaction and polyacrylamide gel electrophoresis, tetranucleotide repeat polymorphism(CCTT and CTT) in the 3`-flanking region of NTR gene was observed. For a comparison of NTR gene`s allelic frequencies between patients with schizophrenia and normal healthy controls, chi-square test and Bonferroni`s correction was performed. Results: The frequency of A10 allele(base pair size=399)was significantly higher in normal healthy controls than schizophrenia(χ²=16.4902, df=1, p<.000). In the comparison between schizophrenic patients with negative symptoms and normal controls, the frequency of A10 allele was significantly higher in normal healthy control subjects than patients with schizophrenia(χ²=21.33, df=1, p<0.001). In the case of male, the frequency of A10 allele of schizophrenia was significantly higher than normal controls(χ²=13.71, df=1, p<0.001). Conclusions: NTR gene was negatively associated with schizophrenia. NTR gene`s tetranucleotide repeat polymorphism may provide some protective function against schizophrenia.

남부 중간지 자운영재배답에서 벼재배시 논 유형에 따른 질소시비량 구명

윤봉기,김병호,김희권,이인,최형국,박인진 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.1

중간지의 보통답과 사질답에서 녹비작물인 자운영을 재배한 논에 벼를 재배할 때 답 유형에 따른 질소비료 시용량을 구명하고자 전남 구례의 보통답과 사질답에서 관행, 자운영+질소 70%, 자운영+질소 50%, 자운영+질소 30%, 자운영+무질소의 5처리로 2000년부터 2년동안 시험한 결과는 다음과 같다. 1. 자운영의 개화성기 생육은 초장 44.1∼44.2㎝, 생초중 20.1∼22.5Mg ㏊^-1이였으며 무기성분 함량은 T-N 2.46∼2.68%, P_2O_5 0.56∼0.60%, K_2O 2.05∼2.18%였다. 사질답에 비하여 보통답에서 자운영의 생초중이 많았다. 2. 토양중 NH_4-N함량의 경시적 변화는 이앙 후 7일에 21.5∼48.3㎎ ㎏^-1으로 가장 높았으며 이앙후 70일에 7.0∼9.7㎎ ㎏^-1으로 점차 감소하였는데 처리에 따라서는 자운영+질소 70%, 자운영+질소 50%시용구에서 높았다. 3. 벼 수확기의 간장 76∼85㎝, 수장 17.9∼19.2㎝로 사질답이 보통답에 비하여 간장, 수장이 컸다. 4. 시험후 토양의 화학성은 pH 5.4∼5.8, OM 21.5∼24.4g ㎏^-1, CEC 9.2∼10.9cmol ㎏^-1, Ava. P_2O_5 141∼360㎎ ㎏^-1으로 자운영처리구에서 OM, CEC함량이 증가하였다. 5. 벼의 수량은 관행(보통답 5.03Mg ㏊^-1, 사질답 5.18Mg ㏊^-1)에 비하여 보통답의 자운영+질소 70%, 50%시용구에서 각각 5, 4%증수되었고, 사질답에서는 자운영+질소 70%, 50%시용구에서 각각 7, 4%증수되었다. 6. 남부 중간지에서 자운영을 재배하여 생초 20Mg ㏊^-1를 녹비로 투입하면 벼를 재배시 질소 시용 적정량은 보통답, 사질답 모두 관행 시비량의 50%이였다. This study was conducted to find out fertilizer recommendation rates for rice at a normal and sandy paddy field after planting milk vetch(MV) in Guryae which lies to southern medium altitude region from 100m to 250m above sea level from 2000 to 2001. The amount of N fertilizer were 110(normal paddy) and 130(sandy paddy) ㎏ ㏊^-1 at control and 0, 30, 50, 70% of the control. At flowering stage of milk vetch, the content of T-N, P_2O_5, K_2O in plant were 2.46∼2.68%, 0.56∼0.60%, 2.05∼2.18%, respectively. Plant height were 44.1∼44.2㎝. Fresh weight were 20.1∼22.5Mg ㏊^-1. Fresh weight of milk vetch cultivated in normal paddy field was more than that in sandy paddy field. The content of NH_4-N in soil was 21.5∼48.3㎎ ㎏^-1 at 7 days after planting and 7.0∼9.7㎎ ㎏^-1 at 70 days after planting. Content of NH_4-N in soil was increased at the treatments of milk vetch + N 70, 50% of control. Culm length was 76∼85㎝, panicle length was 17.9∼19.1㎝ at harvesting stage. Culm length and panicle length at sandy paddy field were longer than those of normal paddy field. Chemical properties of soil after experiment were pH 5.4∼5.8, OM 15.6∼21.5g ㎏^-1, CEC 9.2∼10.9cmol ㎏^-1, Ava. P_2O_5 141∼360㎎㎏^-1, respectively. The content of OM, CEC in soil applied milk vetch at normal paddy field was higher than those of the control, and the content of CEC in soil applied milk vetch at sandy paddy field was higher than that of the control. When milk vetch was cut at flowing stage, rice yield of milk vetch + N 70, 50% of control plot were increased 5, 4% more than that of control(5.03Mg ㏊^-1) at normal paddy field. Rice yield vetch+N 70, 50 of control were increased 7, 4% more than that of control(5.18Mg ㏊^-1) at sandy paddy field. Nitrogen application rate of normal and sandy paddy field was 50% of conventional fertilization rate when 20Mg ㏊^-1 milk vetch cultivated paddy field was inputed for rice culture in the southern medium altitude.

중추에서 Prostaglandin계가 Renin-angiotensin System에 미치는 영향

최영태,김종승,문성호,오형균,김재훈,전제열,염철호,윤평진 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.1

Role of prostaglandins on the renin-angiotensin system in the central nervous system was examined in normotensive and 2-kidney, 1 clip (2K1C) hypertensive rats. The experiment was done under thiopental (50 ㎎/㎏, IP) anesthesia. Captopril and indomethacin were injected into the right lateral cerebral ventricle. Arterial blood pressure and heart rate were recorded from the femoral artery. Intracerebroventricular (ICV) captopril (1 ㎎) caused a decrease in mean arterial pressure in both normotensive and 2K1C hypertensive rats. The depressor response to captopril was more sensitive in hyper-tensive rats than in normotensive rats. Indomethacin treatment (ICV, 200 ㎎) altered the depressor response to captopril neither in normotensive nor in hypertensive rats. These results suggest that the cardiovascular effect of renin-angiotensin system in the central nervous system may not be mediated via prostaglandin systems in normotensive and 2KlC hypertensive rats.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Spread of multidrug-resistant Escherichia coli harboring integron via swine farm waste water treatment plant

Jin-Hyeong Park(Jin-Hyeong Park),Young-Ji Kim(Young-Ji Kim),Binn-Kim(Binn Kim),Kun-Ho Seo(Kun-Ho Seo) 한국예방수의학회 2017 한국예방수의학회 학술대회자료집 Vol.2017 No.-