http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Jiang Ge (검색결과 79 건)
- 무료
- 기관 내 무료
- 유료
-
Insect-specific microRNA involved in the development of the silkworm Bombyx mori
Yong Zhang,Xue Zhou,Xie Ge,Jiang-Hao Jiang,Mu-Wang Li,Shi-Hai Jia,Xiao-Nan Yang,Yun-Chao Kan,Xue-Xia Miao,Guo-Ping Zhao,Fei Li,Yong-Ping Huang 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
MicroRNAs (miRNAs) are endogenous non-coding genes that participate in post-transcription regulation by either degrading mRNA or blocking its translation. It is considered to be very important in regulating insect development and metamorphosis. Insects are the largest group of animals and are extremely valuable in biological and agriculture research. Insects are also important pests to human health and agriculture, and efforts are necessary protect both humans and plants from disease and damage. Despite their importance, insects lag behind mammals, nematodes, and plants in miRNA research. At present, only 279 insect miRNAs have been identified from Drosophila melanogaster, Anopheles gambiae, Apis mellifera, Bombyx mori, and D. pseudoobscura in miRBase, and most of these miRNAs were computationally predicted without experimental validation. Functional analysis of insect miRNAs has only been conducted in D. melanogaster.
-
-
DNA/PEI/ Alginate Polyplex as an Efficient In Vivo Gene Delivery System
Jiang, Ge,Min, Sang-Hyun,Oh, Eun-Ju,Hahn, Sei-Kwang Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.6
A novel non-viral gene delivery system comprised of a DNA/PEI/Alginate (DPA) polyplex was prepared and assessed in vitro and in vivo. Coating the positively charged DNA/PEI (DP) complex with a polyanion resulted in a high level of in vitro reporter gene transfection in the presence of 50 vol% serum due to the minimized cytotoxicity of PEl and the reduced nonspecific interactions with serum components. Among the tested anionic polymers, which included sodium alginate, poly(methacrylic acid) and poly(acrylic acid), the sodium alginate showed the highest gene transfection efficiency. The DPA polyplex also showed a reduced level of erythrocyte aggregation in target cells when compared with the DP complex. According to in vivo studies in which reporter genes encoding green fluorescent protein (GFP) and luciferase were used, injection of the DPA polyplex into tumor cells in six week old female C57/BL6 mice resulted in a much higher level of GFP expression and approximately 7 fold higher luciferase expression than treatment with the DP complex. Taken together, these results demonstrated that the anionic alginate coating of the DP complex contributed to efficient gene delivery in vitro and in vivo.
-
Ge, Jiang,Min, Sang-Hyun,Kim, Dong-Min,Lee, Dong-Chul,Park, Kyung-Chan,Yeom, Young-Il 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
We developed a gene delivery strategy targeting metastatic tumors by exploiting the specific matrix metalloproteinases (MMPs) secreting properties of metastatic tumor cells. A ternary polyplex has been formed by coating polyethylenimine/DNA (PD) complex with an excessive amount of negatively charged gelatin B (GPD-B). We show that GPD-B's gene delivery activity could be targeted to cancer cells via the MMP-mediated proteolytic process, while GPD-A, made from positively charged gelatin A, was not successful in exhibiting such activity. The 1,10-Phenanthroline, an MMP2 inhibitor, abrogated the MMP-dependent transfection activity of GPD-B. GPD-B carried much less positive surface charges than PD, and thus exhibited significantly reduced interactions with erythrocytes. However, MMP2 elevated the positiveness in GPD-B's surface charge and, thus, its interaction with erythrocytes. These results suggest that the anionic gelatin coating may confer improved stabilities on GPD-B in the surrounding medium, while MMP2-mediated disintegration of the gelatin coat enhances the gene delivery to metastatic cancer cells via increasing the likelihood of local charge-mediated interactions between the polyplex and cancer cell membrane.
-
Jiang Ge,민상현,김동민,이동철,박경찬,Young Il Yeom 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
We developed a gene delivery strategy targeting metastatic tumors by exploiting the specific matrix metalloproteinases (MMPs) secreting properties of metastatic tumor cells. A ternary polyplex has been formed by coating polyethylenimine/DNA (PD) complex with an excessive amount of negatively charged gelatin B (GPDB). We show that GPD-B’s gene delivery activity could be targeted to cancer cells via the MMP-mediated proteolytic process, while GPD-A, made from positively charged gelatin A, was not successful in exhibiting such activity. The 1,10-Phenanthroline, an MMP2 inhibitor, abrogated the MMPdependent transfection activity of GPD-B. GPD-B carried much less positive surface charges than PD, and thus exhibited significantly reduced interactions with erythrocytes. However, MMP2 elevated the positiveness in GPDB’s surface charge and, thus, its interaction with erythrocytes. These results suggest that the anionic gelatin coating may confer improved stabilities on GPD-B in the surrounding medium, while MMP2-mediated disintegration of the gelatin coat enhances the gene delivery to metastatic cancer cells via increasing the likelihood of local chargemediated interactions between the polyplex and cancer cell membrane.
-
-
경구용 백신수송체용 GFP 함유 마이크로스피어의 제조 및 평가
장혁(Ge Jiang),박종필(Jong Pil Park),곽손혁(Son Hyok Kwak),황성주(Sung Joo Hwang),맹필재(Pil Jae Maeng) 한국약제학회 2000 Journal of Pharmaceutical Investigation Vol.30 No.4
In order to design the oral vaccine delivery system, we prepared the alginate microspheres containing GFP (green fluorescent protein) as a model drug by spray method. To optimize the preparation conditions of microspheres, we investigated the effects of various parameters including nozzle pressure, nozzle opening angle, and concentrations of sodium alginate and calcium chloride. The prepared microspheres were evaluated by measuring their sizes, loading efficiency, and morphology. The particle size of microspheres was affected by the concentration of sodium alginate and calcium chloride, nozzle pressure, and nozzle opening angle. As the concentration of sodium alginate increased, GFP loading efficiency and particles size of microsphere also increased. However, it was observed to be difficult to spray the sodium alginate solution with concentration greater than 1.5% (w/v), due to high viscosity. The pressure over 3 kgf/㎠ didn`t affect the size of particles. As a result, the spraying method enabled us to prepare microspheres for oral vaccine delivery system. In this study, microspheres prepared with 1% (w/v) sodium alginate had greater loading efficiency and better spherical shape.
-
Ming Jiang,Yunfei Li,Yuan Ge,Wengeng Gao,Ke Lou,Shinong Wang,Juanjuan Jiang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.12
Position information is the foundation of massive applications in Wireless Sensor Network(WSN). Three improved positioning algorithms based on DV-Hop are proposed in order to enhance the positioning accuracy of wireless sensor nodes. First improved algorithm is distance compensation algorithm (DCA) that creates a triangle model to compensate the estimated distance. The second improved algorithm creates a new chain table for all anchor nodes to record and compute the average hop distance. The third improved algorithm is weighting different anchor nodes with anchor nodes’ nearest unknown nodes. The second and third improved algorithms are based on the DCA. The simulation results show that the three improved algorithms are better than the original DV-Hop in localization accuracy. Compared to the original DV-Hop algorithm, the simulation results shows that the three improved algorithms proposed in the paper increase the positioning accuracy of the unknown nodes.
-
Upregulation and biological function of transmembrane protein 119 in osteosarcoma
Zhen-Huan Jiang,Jun Peng,Hui-Lin Yang,Xing-Li Fu,Jin-Zhi Wang,Lei Liu,Jian-Nong Jiang,Yong-Fei Tan,Zhi-Jun Ge 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Osteosarcoma is suggested to be caused by genetic and molecular alterations that disrupt osteoblast differentiation. Recent studies have reported that transmembrane protein 119 (TMEM119) contributes to osteoblast differentiation and bone development. However, the level of TMEM119 expression and its roles in osteosarcoma have not yet been elucidated. In the present study, TMEM119 mRNA and protein expression was found to be up-regulated in osteosarcoma compared with normal bone cyst tissues. The level of TMEM119 protein expression was strongly associated with tumor size, clinical stage, distant metastasis and overall survival time. Moreover, gene set enrichment analysis (GSEA) of the Gene Expression Omnibus (GEO) GSE42352 dataset revealed TMEM119 expression in osteosarcoma tissues to be positively correlated with cell cycle, apoptosis, metastasis and TGF-β signaling. We then knocked down TMEM119 expression in U2OS and MG63 cells using small interfering RNA, which revealed that downregulation of TMEM119 could inhibit the proliferation of osteosarcoma cells by inducing cell cycle arrest in G0/G1 phase and apoptosis. We also found that TMEM119 knockdown significantly inhibited cell migration and invasion, and decreased the expression of TGF-β pathway-related factors (BMP2, BMP7 and TGF-β). TGF-β application rescued the inhibitory effects of TMEM119 knockdown on osteosarcoma cell migration and invasion. Further in vitro experiments with a TGF-β inhibitor (SB431542) or BMP inhibitor (dorsomorphin) suggested that TMEM119 significantly promotes cell migration and invasion, partly through TGF-β/BMP signaling. In conclusion, our data support the notion that TMEM119 contributes to the proliferation, migration and invasion of osteosarcoma cells, and functions as an oncogene in osteosarcoma.
-
Chemical processing of water-soluble species and formation of secondary organic aerosol in fogs
Kim, Hwajin,Collier, Sonya,Ge, Xinlei,Xu, Jianzhong,Sun, Yele,Jiang, Wenqing,Wang, Youliang,Herckes, Pierre,Zhang, Qi Elsevier 2019 Atmospheric environment Vol.200 No.-
<P><B>Abstract</B></P> <P>A field study on fog chemistry and aqueous-phase processing of aerosol particles was conducted in Fresno, California's San Joaquin Valley (SJV) during wintertime. Fog droplets were collected while interstitial submicron aerosol was characterized in real time using a High Resolution Time-of-Flight Aerosol Mass Spectrometer (HR-AMS). The fog samples were later analyzed using HR-AMS, ion chromatography (IC), and total organic carbon analyzer (TOC). Compared to interstitial aerosol, dissolved solutes in fog waters were composed of higher fractions of ammonium, nitrate, sulfate, methanesulfonic acid, and oxygenated organic compounds, likely due to aqueous formation of secondary species as well as enhanced gas-to-particle partitioning of water-soluble gases under humid conditions. The low-volatility dissolved organic matter in fog water (F-OA) was moderately oxidized with an average oxygen-to-carbon (O/C) ratio of 0.42. The chemical composition of F-OA appeared to be overall similar to that of oxygenated organic component in interstitial aerosol (OOA) and the HR-AMS mass spectra of F-OA and OOA are highly similar (r<SUP>2</SUP> > 0.95). However, there are also significant chemical differences as F-OA appeared to contain a larger fraction of carboxylic functional groups than OOA, indicating enhanced organic acid formation through aqueous-phase reactions. In addition, F-OA was composed of substantially more nitrogen-containing compounds, with an average N/C ratio ∼4 times that of OOA. Most strikingly was that the F-OA spectra showed substantial enhancements of the C<SUB>x</SUB>H<SUB>y</SUB>N<SUB>2</SUB> <SUP>+</SUP> (x ≥ 0; y ≥ 0) ions, which were likely contributed by imidazole- and/or pyrazine-based compounds formed from the aqueous reactions of aldehydes with amino compounds. The results of this study demonstrated that aqueous reactions in atmospheric droplets can significantly modify aerosol composition and contribute to the formation of oxygenated and nitrogen-containing organic compounds in atmospheric aerosol particles. This finding is important for understanding aerosol's effects on human health, air quality, and climate.</P> <P><B>Highlights</B></P> <P> <UL> <LI> HR-AMS study of fog processing of PM1 in San Joaquin Valley of CA during winter. </LI> <LI> Fog waters are enriched of secondary inorganic and organic aerosol species. </LI> <LI> Organic residuals in fog waters (FOM) is moderately oxidized (average O/C = 0.42). </LI> <LI> FOM has more abundant carboxylic acid and organic nitrogen compounds than OOA. </LI> <LI> This is evidence for forming imidazole- or pyrazine-based compounds in fog waters. </LI> </UL> </P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
