대학생의 우울정도 : 간호학전공과 타전공대학생을 중심으로

김지연,류나은,이소라,이지희,정윤정,주지연,황인혜 이화여자대학교 간호과학대학 2012 이화간호학회지 Vol.- No.46

Purpose: This study was designed to investigate sample’s general characteristics and compare depression degree among sample’s general characteristics and students’ major(nursing vs. other major). Methods: The subjects consisted of 318 university students. Data was collected by self-reported questionnaires, which were constructed BDI score. Data was analyzed by the SPSS/PC WIN 19.0 program. Results: The depression of nursing students and other major students was not significantly different according to BDI score. Statistically significant difference was identified among sleeping. Conclusion: Specific study focused on the practice time should be done to confirm the depression of nursing major and other majors. Detailed support programs which specifically deal with sleeping should be developed to effectively reduce the harmful effects of individual vulnerability. Therefore, prevention and management system to reduce depression degree for university students is needed.

골격성 Ⅲ 급 부정교합자의 두개안모 성장예측에 대한 평가

손우성,강은희,정미라,성지현 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.1

본 연구는 성장에 의한 변화를 예측하는 현재의 방법이 실제로 골격성 Ⅲ급 부정교합자의 진단과 치료계획에 적절히 사용될 수 있는 지를 판단해 보기 위해 초진 시 골격성 Ⅲ급 부정교합으로 진단받고 성장 종료 후 악교정 수술을 받기위해 재내원한 골격성 Ⅲ 급 부정교합자 25명(남자 13명, 여자 12명)을 대상으로 시행되었다. 초진시 채득된 측모 두부방사선 사진에서 Ricketts의 성장예측법을 통해 성장 종료 후의 상태를 예측한 후, 악교정 수술을 위해 재내원 했을 때 채득된 측모 두부방사선 사진에서 계측된 실제 성장량과 비교분석 함으로써 다음과 같은 결론을 얻었다. 1. 예측치와 관측치의 일치정도를 평가해본 결과 Porion Location, Ramus Position, Facial Depth, Facial Axis, Mandibular Plane angle, Maxillary Convexity에서의 예측치가 관측치와 차이를 보이고 있어 이 항목에 대한 Ricketts 성장예측법의 예측치가 실제 성장량을 잘 설명해주지 못함이 관찰되었다. 2. 하악골체의 성장량은 정상 성장량과 유사하였으나 Porion Location, Ramus Position이 정상적인 성장과정을 벗어나면서 하악골의 전방위치를 유도하였다. 3. 골격성 Ⅲ 급 부정교합자에서는 성장의 양과 방향이 비정상적인 성장변화를 나타내어 정상 성장에서라면 변화를 보이지 않을 하악지의 전방 위치, 하악이부의 전상방 회전 등이 일어난 하악 전돌의 경향이 악화될 수 있다. This study was performed to evaluate whether growth prediction method can be used to diagnose and make treatment plan in skeletal Class Ⅲ malocclusion patients or not. The sample was consisted of 25 patients(13 males, 12 females) who had been diagnosed with skeletal Class Ⅲ malocclusion at first visit and after that had returned to take orthognathic surgery. Growth prediction performed with Ricketts' growth prediction method from first cephalogram was compared with actual growth of the second cephalogram. The findings of this study were as follows ; 1. There was significant difference between actual growth and growth prediction in Porion Location, Ramus Position, Facial Depth, Facial Axis, Mandibular Plane angle, Maxillary Convexity. So, for these items Ricketts' growth prediction method is not proper to predict growth. 2. Although the growth amount of mandibular body was similar to normal growth amount, mandible was positioned anteriorly because of Porion Location and Ramus Position. 3. In skeletal Class Ⅲ malocclusion patients, the tendency of mandibular prognathism might be aggreviated because of anterior placement of ramus and anterosuperior rotation of pogonion.

Lipid-Lowering Effects of Sunchang Kochujang in Hyperlipidemic Humans

Eun-Soo Jung,Mi-Ra Oh,Soo-Hyun Park,Eun-Ock Park,Ji-Hee Lim,Su-Young Jung,Eun-Kyung Choi,Soo-Wan Chae 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10

Antioxidative Effect and Neuraminidase Inhibitory Activity of Polyphenols Isolated from a New Korean Red Waxy Sorghum (Sorghum bicolor L. cv. Hwanggeumchalsusu)

Ji-Eun Ra(나지은),Kyung Hye Seo(서경혜),Jee Yeon Ko(고지연),Mi-Ja Lee(이미자),Hyeon Jung Kang(강현중),Sun Lim Kim(김선림),Ill-Min Chung(정일민),Woo Duck Seo(서우덕) 한국생명과학회 2015 생명과학회지 Vol.25 No.7

본 연구는 황금찰수수의 에탄올 추출물을 이용하여 항산화 활성 및 뉴라니미데이즈 억제 효과를 조사하였다. 헥산, 에틸아세테이트, 메탄올과 70% 에탄올 조추출물에서 항산화력의 IC50값을 비교한 결과 70% 에탄올 추출물 이 DPPH 라디칼 소거능, ABTS 라디칼 소거능에서 각각 83.2±2.7, 85.6±2.4 μg/ml으로 가장 높았고, 뉴라니미데이즈 억제활성 IC50값을 비교한 결과 메탄올 추출물에서 1.8±0.1 μg/ml로 나타났다. 또한 황금찰수수 추출물에서 컬럼크로마토그래피와 UPLC-PDA-MS/MS 분광기 분석을 통해, Gallic acid (1), Protocatecuic acid (2), p-Hydroxy benzoic acid (3), Vanillic acid (4), Caffeic acid (5), Ferulic acid (6), Luteolinidin (7), Apigeninidin (8), Luteolin (9), 총 9종의 폴리페놀 화합물을 확인하였다. 또한 각각의 화합물에 대한 항산화력의 IC50값을 비교한 결과 Luteolinidin이 DPPH 라디칼 소거능, ABTS 라디칼 소거능에서 각각 10.9±0.5, 8.6±3.0 μM로 가장 우수 하였고, 뉴라니미데이즈 억제활성은 Luteolin이 12.9±3.8의 IC50값과 비경쟁적 저해모델을 보였다. 결과적으로 황금찰수수는 높은 항산화 효과와 뉴라미니데이즈 억제활성을 보여 식품, 사료 등의 새로운 기능성소재로 다양하게 활용될 수 있음을 시사한다. To identify nutritional and therapeutic properties of the new Korean red waxy sorghum cultivar ‘Hwanggeumchalsusu (HGC)’, we assayed the antioxidative effects and neuraminidase inhibitory activity. A methanol and 70% ethanol extract of HGC exhibited strong antioxidative effects (IC50 values of 83.2±2.7 for DPPH) and 85.6±2.4 μg/ml for ABTS) and neuraminidase (ND) inhibitory activity (IC50 values of 1.8±0.1 from extracted with methanol and 3.4±0.1 μg/ml from extracted with 70% ethanol) compared with that of the control, noncolored sorghum cultivar ‘Huinchalsusu (HC)’ (IC50 > 200μg/ml). We isolated nine polyphenols, Gallic acid (1), Protocatecuic acid (2), p-Hydroxy benzoic acid (3), Vanillic acid (4), Caffeic acid (5), Ferulic acid (6), Luteolinidin (7), Apigeninidin (8), Luteolin (9), from the HGC?methanol extract, to determine whether they were the active components Luteolinidin of one kind of polyphenols from the HGC, exhibited significant antioxidative effects (IC50 values of 10.9±0.5 μM for DPPH and 8.6 0.6 μM for ABTS) and neuraminidase (ND) inhibitory activity (IC50 values of 26.3±0.6) showed noncompetitive inhibition model. The binding affinity of the ND inhibitors in molecular docking experiments correlated with their ND inhibitory activities. These results suggest that HGC may be utilized to serve as a potential effective antioxidant and inhibitor of ND.

Simvastatin as a Modulator of Tissue Remodeling through Inhibition of Matrix Metalloproteinase (MMP) Release from Human Lung Fibroblasts

( Ji Eun Ra ),( Ji Kyoung Lee ),( Hui Jung Kim ) 대한결핵 및 호흡기학회 2011 Tuberculosis and Respiratory Diseases Vol.71 No.3

Background: Statins can regulate the production of pro-inflammatory cytokines and inhibit MMP production or activation in a variety of types of cells. This study evaluated whether statins would inhibit MMP release from human lung fibroblasts, which play a major role in remodeling processes. Methods: This study, using an in-vitro model (three-dimensional collagen gel contraction system), evaluated the effect of cytokines (tumor necrosis factor-α, TNF-a and interleukin-1β, IL-1b) on the MMP release and MMP activation from human lung fibroblasts. Collagen degradation induced by cytokines and neutrophil elastase (NE) was evaluated by quantifying hydroxyproline. Results: In three-dimensional collagen gel cultures (3D cultures) where cytokines (TNF-a and IL-1b) can induce the production of MMPs by fibroblasts, it was found that simvastatin inhibited MMP release. In 3D cultures, cytokines together with NE induced collagen degradation and can lead to activation of the MMP, which was inhibited by simvastatin. Conclusion: Simvastatin may play a role in regulating human lung fibroblast functions in repair and remodeling processes by inhibiting MMP release and the conversion from the latent to the active form of MMP.

Simvastatin as a Modulator of Tissue Remodeling through Inhibition of Matrix Metalloproteinase (MMP) Release from Human Lung Fibroblasts

Ra, Ji-Eun,Lee, Ji-Kyoung,Kim, Hui-Jung The Korean Academy of Tuberculosis and Respiratory 2011 Tuberculosis and Respiratory Diseases Vol.71 No.3

Background: Statins can regulate the production of pro-inflammatory cytokines and inhibit MMP production or activation in a variety of types of cells. This study evaluated whether statins would inhibit MMP release from human lung fibroblasts, which play a major role in remodeling processes. Methods: This study, using an in-vitro model (three-dimensional collagen gel contraction system), evaluated the effect of cytokines (tumor necrosis factor-${\alpha}$, TNF-a and interleukin-$1{\beta}$, IL-1b) on the MMP release and MMP activation from human lung fibroblasts. Collagen degradation induced by cytokines and neutrophil elastase (NE) was evaluated by quantifying hydroxyproline. Results: In three-dimensional collagen gel cultures (3D cultures) where cytokines (TNF-a and IL-1b) can induce the production of MMPs by fibroblasts, it was found that simvastatin inhibited MMP release. In 3D cultures, cytokines together with NE induced collagen degradation and can lead to activation of the MMP, which was inhibited by simvastatin. Conclusion: Simvastatin may play a role in regulating human lung fibroblast functions in repair and remodeling processes by inhibiting MMP release and the conversion from the latent to the active form of MMP.

Molecular epidemiological study on a human patient of Brucella melitensis infection occurred in Korea

Eun Ji Yum,Jin-Ju Lee,Min-Kyeong Kim,Eun-Ji Park,So-Ra Sung,Min-Hoe Lee,Soon-Seek Yoon,Moon Her,Jeong-Soo Choi 한국예방수의학회 2019 예방수의학회지 Vol.43 No.4

Brucellosis is the most common zoonosis worldwide, which is caused by Brucella spp. In humans, it can be mainly occurred by direct contact with infected animals or consumption of contaminated dairy products. This study focused on human brucellosis caused by B. melitensis discovered from Chinese worker in Korea in 2015. We investigated molecular epidemiological evidence to find the infection source. We first performed several PCR methods including 16S rRNA PCR, multiplex PCR and real-time PCR to identify Brucella species. We also conducted MLVA typing for epidemiological trace-back analysis. The isolate from the patient was confirmed to B. melitensis through Brucella-specific PCR. In clustering analysis with B. melitensis from foreign countries, this human isolate was correlated with B. melitensis isolates from humans and sheep in China by 99.9% similarity. Thus, we assumed the brucellosis patient has been already infected in China followed by migration to Korea according to molecular epidemiological analysis with history evidence. Moreover, we suggest it needs to take measures to reduce the risk for intercountry transmission of brucellosis due to the influx of infected people from abroad.

Evaluation of antihypertensive polyphenols of barley (Hordeum vulgare L.) seedlings via their effects on angiotensin-converting enzyme (ACE) inhibition

Ra Ji-Eun,Woo So-Yeun,Jin Hui,Lee Mi Ja,Kim Hyun Young,Ham Hyeonmi,Chung Ill-Min,Seo Woo Duck 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.4

Angiotensin-converting enzyme (ACE) is an important therapeutic target in the regulation of high blood pressure. This study was conducted to investigate the alterations in blood pressure associated with ACE inhibition activity of the polyphenols (1–10), including 3-O-feruloylquinic acid (1), lutonarin (2), saponarin (3), isoorientin (4), orientin (5), isovitexin (6), isoorientin-7-O-[6-sinapoyl]-glucoside (7), isoorientin-7-O-[6-feruloyl]-glucoside (8), isovitexin-7-O- [6-sinapoyl]-glucoside (9), and isovitexin-7-O-[6-feruloyl]-glucoside (10), isolated from barley seedlings (BS). All the isolated polyphenols exhibited comparable IC50 values of ACE inhibition activity (7.3–43.8 μM) with quercetin (25.2 ± 0.2 μM) as a positive control, and their inhibition kinetic models were identified as noncompetitive inhibition. Especially, compound 4 was revealed to be an outstanding ACE inhibitor ( IC50 = 7.3 ± 0.1 μM, Ki = 6.6 ± 0.1 μM). Based on the compound structure–activity relationships, the free hydroxyl groups of flavone-moieties and glucose connections at the A ring of the flavone moieties were important factors for inhibition of ACE. The alcohol extract of BS also demonstrated potent ACE inhibition activity (66.5% ± 2.2% at 5000 μg mL−1). The polyphenols from BS had strong inhibitory activity on ACE and this study results suggest that BS can be used as an effective blood pressure regulator through ACE inhibition.

The Effect of Kochujang pill on in hyperlipidemia Subjects

Ji-Hee Lim,Mi-Ra Oh,Soo-Hyun Park,Eun-Ock Park,Eun-Soo Jung,Yoon-Jin Jang,Soo-Young Jung,Eun-Kyung Choi,Do-Yeoun Jeong,Seung-Wha Jo,Soo-Wan Chae 한국식품영양과학회 2013 한국식품영양과학회 학술대회발표집 Vol.2013 No.11

The Effect of Mat´e Extract Capsules on in Obesity Subjects

Ji-Hee Lim,Sun-Young Kim,Mi-Ra Oh,Soo-Hyun Park,Eun-Ock Park,Eun-Soo Jung,Eun-Kyung Choi,Soo-Wan Chae,Min-Gul Kim 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10