http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Jeongwook Heo ),( Kyounghoon Park ),( Chanheum Park ),( Jaesu Lee ),( Jeonghyun Baek ),( Seouggil Hong ),( Gongin Lee ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
In hydroponic culture, several nutrient solutions were used for plant growth and development under smart greenhouse (Plant factory system) conditions with artificial lights instead of sun light. The conventional solutions are automatically irrigated for leafy plants production under controlled EC and pH conditions. Researches on the nutrient solution control were focused on the cool-season leafy plants such as lettuce spp. growing for 4~5 weeks after seedling transplantation on hydroponic culture bed. However, investigations on the effects of nutrient solutions for long term culture crops compared to the leafy plants have not been conducted. It was conducted to investigate the effect of conventional culture mediums on long-term growth of Ledebouriella seseloides seedlings under smart greenhouse conditions. Conventional Japan Wonsi standard (JW), Yamazaki Leaf lettuce (YL), and Yamazaki Padeudeuk (YP) solutions were used for hydroponic culture. The seedlings with a longer growth period than leafy plants were considered as a plant material. The seedlings which developed 2~3 true leaves were grown in the smart greenhouse with Light-Emitting Diodes (LEDs) lights of blue, red and white of 1:2:1 mixture in energy ratio. The three nutrient solutions were regularly irrigated by the deep flow technique (DFT) system on the culture gutters for 350 days. Long term culture of Ledebouriella seseloides seedlings with a longer growth period was established by the nutrient solutions which conventionally used for hydroponic culture of leafy plants inside the smart greenhouse conditions. There was no significance in the leaf growth of the seedlings such as fresh weight, leaf extension and leaf pigmentation among the mediums. From the results, it is suggested that the type of culture mediums was not crucial factor in long-term growth of the seedlings under controlled environments such as smart greenhouse conditions although average leaf number of the seedlings grown under MJ or YP treatment significantly increased.
블루투스 비콘 센서 노드를 활용한 스마트 안전 계측 시스템 설계
박영수 ( Young-soo Park ),박창진 ( Chang-jin Park ),조선희 ( Sun-hee Cho ),박경용 ( Kyoung-yong Park ),김민선 ( Min-sun Kim ),서정욱 ( Jeongwook Seo ) 한국항행학회 2017 韓國航行學會論文誌 Vol.21 No.1
본 논문은 오래된 옹벽과 같은 건축물이나 잠재적 위험을 내재한 건설 및 토목 구조물 등에 부착 가능한 블루투스 비콘 센서 노드를 활용하여 위험감지 및 대피/대처 서비스를 제공해줄 수 있는 스마트 안전 계측 시스템을 설계한다. 제안한 시스템은 블루투스 비콘을 활용하여 비콘이 설치된 지점의 가속도, 자이로, 지자계, 압력/고도, 온도/습도 등의 다양한 센서 데이터를 전송할 수 있으며 이러한 데이터를 활용하여 위험 감지, 예측, 경고 등의 다양한 서비스에 활용할 수 있다. 제안한 시스템의 실효성을 검증하기 위해 필드 테스트를 수행하였으며 옹벽의 변위값이 허용변위 38.5 mm 내에 존재한다는 것을 확인하였다. This paper designs a smart safety measurement system with Bluetooth beacon sensor nodes that can provide risk detection and evacuation/countermeasure services. The Bluetooth beacon sensor nodes is easily able to be attached to old building wall or construction or civil structure with potential danger. The proposed smart safety measurement system transmits various sensor data such as acceleration, gyroscope, geomagnetic, pressure, altitude, temperature, humidity at the spot where Bluetooth beacon sensor nodes are installed, and we can use them for risk perception, prediction, and warning services. To verify the effectiveness of the proposed system, we performed filed tests which showed that measured displacement values of precast retaining walls were within the permitted displacement value of 38.5 mm.
Park, Seonhwa,Jeong, Ji-Eun,Le, Van Sang,Seo, Jeongwook,Yu, Byeongjun,Kim, Da-Young,Kwon, Se-Hun,Jon, Sangyong,Woo, Han Young,Yang, Haesik American Chemical Society 2018 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.140 No.7
<P>Direct electron transfer between a redox label and an electrode requires a short working distance (<1–2 nm), and in general an affinity biosensor based on direct electron transfer requires a finely smoothed Au electrode to support efficient target binding. Here we report that direct electron transfer over a longer working distance is possible between (i) an anionic π-conjugated polyelectrolyte (CPE) label having many redox-active sites and (ii) a readily prepared, thin polymeric monolayer-modified indium–tin oxide electrode. In addition, the long CPE label (∼18 nm for 10 kDa) can approach the electrode within the working distance after sandwich-type target-specific binding, and fast CPE-mediated oxidation of ammonia borane along the entire CPE backbone affords high signal amplification.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2018/jacsat.2018.140.issue-7/jacs.7b12382/production/images/medium/ja-2017-12382z_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja7b12382'>ACS Electronic Supporting Info</A></P>
Park, Seonhwa,Kim, Jihye,Ock, Hwiseok,Dutta, Gorachand,Seo, Jeongwook,Shin, Eui-Cheol,Yang, Haesik The Royal Society of Chemistry 2015 The Analyst Vol.140 No.16
<P>Washing processes cannot fully remove interfering species that remain on biosensing surfaces when a sample solution contains a high concentration of interfering species. This study reports an immunosensing scheme employing electroreduction-based electrochemical–chemical (EC) redox cycling that allows sensitive detection of vaccinia virus (VV) in a solution containing a high concentration of <SMALL>L</SMALL>-ascorbic acid (AA). To obtain high signal amplification, an enzymatic reaction by β-<SMALL>D</SMALL>-galactosidase (Gal) is combined with electroreduction-based EC redox cycling by an oxidant. Among the four possible oxidants (KIO<SUB>3</SUB>, NaClO, Ag<SUB>2</SUB>O, and H<SUB>2</SUB>O<SUB>2</SUB>), KIO<SUB>3</SUB> shows the highest signal-to-background ratio and is chosen. During an incubation period of 10 min, Gal converts β-<SMALL>D</SMALL>-galactopyranoside into <I>p</I>-aminophenol (AP), which is oxidized to <I>p</I>-quinone imine (QI) by KIO<SUB>3</SUB>. When −0.05 V <I>vs.</I> Ag/AgCl is applied to an immunosensing electrode, QI is reduced to AP, and the regenerated AP is then reoxidized by KIO<SUB>3</SUB>. The electroreduction-based EC redox cycling is induced. An indium−tin oxide electrode modified with reduced graphene oxide and an applied potential of −0.05 V are used to achieve low and reproducible background currents, slow O<SUB>2</SUB> reduction, and fast electroreduction of QI. KIO<SUB>3</SUB> favorably converts AA into noninterfering species during the incubation period. The detection limit for VV in commercial 50% mandarin juice (AA concentration = 0.7 mM) is 4 × 10<SUP>3</SUP> plaque-forming unit (PFU) per mL. The new EC redox cycling scheme is promising for sensitive detection of proteins, viruses, and bacteria in solutions containing high concentrations of AA.</P> <P>Graphic Abstract</P><P>A new redox cycling scheme allows sensitive detection of vaccinia virus in a solution containing a high concentration of <SMALL>L</SMALL>-ascorbic acid. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5an01086a'> </P>
Park, Seonhwa,Kim, Gamwoo,Seo, Jeongwook,Yang, Haesik American Chemical Society 2016 ANALYTICAL CHEMISTRY - Vol.88 No.24
<P>The development of a fast and ultrasensitive protease detection method is a challenging task. This paper reports ultrasensitive protease sensors exploiting (i) selective affinity binding, (ii) selective proteolytic reaction, and (iii) proximity-dependent electrochemical reaction. The selective affinity binding to capture IgG increases the concentration of the target protease (trypsin as a model protease) near the electrode, and the selective proteolytic reaction by trypsin increases the concentration of the redox-active species near the electrode. The electrochemical reaction, which is more sensitive to the concentration of the redox-active species near the electrode than to its bulk concentration, provides an increased electrochemical signal, which is further amplified by the electrochemical chemical redox cycling. An indium tin oxide electrode modified with reduced graphene oxide, avidin, and biotinylated capture IgG is used as the electrode, and p-aminophenol liberated from an oligopeptide is used as the redox-active species. The new sensor scheme using no washing process is compared with the new sensor scheme using washing process, and with the conventional scheme using only proteolytic reaction. The new scheme provides a higher signal-to-background ratio and a lower detection limit. Moreover, the increased electrochemical signal offers a more selective protease detection. Trypsin can be detected in phosphate-buffered saline and in artificial serum containing L-ascorbic acid with a low detection limit of 0.5 pg/mL, over a wide range of concentrations, and with an incubation period of only 30 min without washing process. The washing-free electrochemical protease sensor is highly promising for simple, fast, ultrasensitive, and selective point-of-care testing of low abundance proteases.</P>
A highly sensitive and simply operated protease sensor toward point-of-care testing
Park, Seonhwa,Shin, Yu Mi,Seo, Jeongwook,Song, Ji-Joon,Yang, Haesik The Royal Society of Chemistry 2016 The Analyst Vol.141 No.8
<P>Protease sensors for point-of-care testing (POCT) require simple operation, a detection period of less than 20 minutes, and a detection limit of less than 1 ng mL(-1). However, it is difficult to meet these requirements with protease sensors that are based on proteolytic cleavage. This paper reports a highly reproducible protease sensor that allows the sensitive and simple electrochemical detection of the botulinum neurotoxin type E light chain (BoNT/E-LC), which is obtained using (i) low nonspecific adsorption, (ii) high signal-to-background ratio, and (iii) one-step solution treatment. The BoNT/E-LC detection is based on two-step proteolytic cleavage using BoNT/E-LC (endopeptidase) and L-leucine-aminopeptidase (LAP, exopeptidase). Indium-tin oxide (ITO) electrodes are modified partially with reduced graphene oxide (rGO) to increase their electrocatalytic activities. Avidin is then adsorbed on the electrodes to minimize the nonspecific adsorption of proteases. Low nonspecific adsorption allows a highly reproducible sensor response. Electrochemical-chemical (EC) redox cycling involving p-aminophenol (AP) and dithiothreitol (DTT) is performed to obtain a high signal-to-background ratio. After adding a C-terminally AP-labeled oligopeptide, DTT, and LAP simultaneously to a sample solution, no further treatment of the solution is necessary during detection. The detection limits of BoNT/E-LC in phosphate-buffered saline are 0.1 ng mL(-1) for an incubation period of 15 min and 5 fg mL(-1) for an incubation period of 4 h. The detection limit in commercial bottled water is 1 ng mL(-1) for an incubation period of 15 min. The developed sensor is selective to BoNT/E-LC among the four types of BoNTs tested. These results indicate that the protease sensor meets the requirements for POCT.</P>