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어머니의 양육스트레스와 유아의 외현화 문제행동 및 학교준비도가 초등학교 1학년 학교적응에 미치는 영향
박정현 ( Park¸ Jeonghyeon ),이경님 ( Lee¸ Kyungnim ) 열린부모교육학회 2020 열린부모교육연구 Vol.12 No.4
본 연구는 어머니의 양육스트레스와 유아의 외현화 문제행동 및 학교준비도가 초등학교 1학년 학교적응에 어떠한 직·간접적 영향을 미치는지를 살펴보고자 하였다. 이를 위하여 한국아동패널 7차년도와 8차년도의 종단 자료 중 949명의 유아와 그들의 어머니를 대상으로 수집된 자료를 활용하여 Pearson의 적률상관분석과 구조방정식모형(SEM)분석을 실시하였다. 그 결과 첫째, 유아의 외현화 문제행동과 학교준비도는 초등학교 1학년 학교적응에 직접적 영향을 미치는 것으로 나타났다. 둘째, 유아의 외현화 문제행동은 학교준비도를 매개로 하여 초등학교 1학년 학교적응에 간접적 영향도 미치는 것으로 나타났다. 셋째, 어머니의 양육스트레스가 초등학교 1학년 학교적응에 영향을 미치는 경로에서 유아의 외현화 문제행동과 학교준비도의 순차적인 이중 매개효과 나타났다. 즉 어머니의 양육스트레스는 유아의 외현화 문제행동과 학교준비도를 순차적으로 매개하여 학교적응에 간접적 영향을 미치는 것으로 나타났다. 추가하여 초등학교 1학년 학교적응에 유아의 외현화 문제행동의 영향이 가장 큰 것으로 나타났다. 이러한 결과는 초등학교 1학년 학교적응을 위하여 유아의 외현화 문제행동에 대한 중재와 예방이 필수적임을 강조하고 학교준비도 증진을 위한 지도방안에 초점을 맞춘 교사교육 및 어머니의 양육스트레스 완화를 위한 부모교육이나 사회적 지원이 효과적임을 시사하고 있다. The purpose of this study was to examine the structural relationships among mother’s parenting stress, young children’s externalizing behavioral problems, school readiness and school adjustment in first grade. For this study, seventh(2014) and eighth(2015) data from the Panel Study on Korean Children (PSKC) were analysed by means of Pearson’s correlational relationships and structural equational modelling. The main results were as follows: first, young children’s externalizing behavioral problems and school readiness were found to have a direct effect on school adjustment in first grade. Second, young children’s externalizing behavioral problems was found to have an indirect effect on school adjustment in first grade through school readiness. Third, mother’s parenting stress was found to have an indirect effect on school adjustment in first grade through externalizing behavioral problems and school readiness. Additionally externalizing behavioral problems was found to be the most important variable predicting young children’s school adjustment in first grade. These results suggested that reducing externalizing behavioral problems and mother’s parenting stress, improving school readiness could help young children’s school adjustment in first grade.
Jeonghyeon Moon,Seung Hoon Lee,Seon-yeong Lee,Jaeyoon Ryu,Jooyeon Jhun,JeongWon Choi,Gyoung Nyun Kim,노상호,박성환,조미라 대한면역학회 2020 Immune Network Vol.20 No.5
The protein encoded by the Gene Associated with Retinoid-Interferon-Induced Mortality-19 (GRIM-19) is located in the mitochondrial inner membrane and is homologous to the NADH dehydrogenase 1-alpha subcomplex subunit 13 of the electron transport chain. Multiple sclerosis (MS) is a demyelinating disease that damages the brain and spinal cord. Although both the cause and mechanism of MS progression remain unclear, it is accepted that an immune disorder is involved. We explored whether GRIM-19 ameliorated MS by increasing the levels of inflammatory cytokines and immune cells; we used a mouse model of experimental autoimmune encephalomyelitis (EAE) to this end. Six-to-eight-week-old male C57BL/6, IFNγ-knockout (KO), and GRIM-19 transgenic mice were used; EAE was induced in all strains. A GRIM-19 overexpression vector (GRIM19 OVN) was electrophoretically injected intravenously. The levels of Th1 and Th17 cells were measured via flow cytometry, immunofluorescence, and immunohistochemical analysis. IL-17A and IFNγ expression levels were assessed via ELISA and quantitative PCR. IL-17A expression decreased and IFNγ expression increased in EAE mice that received injections of the GRIM19 OVN. GRIM-19 transgenic mice expressed more IFNγ than did wild-type mice; this inhibited EAE development. However, the effect of GRIM-19 overexpression on the EAE of IFNγ-KO mice did not differ from that of the empty vector. GRIM-19 expression was therapeutic for EAE mice, elevating the IFNγ level. GRIM-19 regulated the Th17/Treg cell balance.
Jeonghyeon Moon,Sangho Roh 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11
Polo-like kinase 1 (Plk1) has multiple roles in somatic cell and mammalian oocyte division. In mice, Plk1 distributes to the centromeres from prophase to anaphase and compose spindle apparatus in mitosis stages. Somatic cell nuclear transfer (SCNT) has diverse advantages. However, low cloning efficiency of SCNT procedure causes difficulty to application. The causes of this low efficiency are still unclear. However, they are attributed to the cumulative results of several biological and technical factors. In this study, Plk1, a biological factor, was investigated. B6D2F1 mice (7 weeks old) were superovulated with 10 IU of pregnant mare’s serum gonadotropin and 9 U of human chorionic gonadotropin (HCG) 48 hr later. The oocytes were collected 14 hr after HCG injection and cultured on potassium simplex optimized medium. The BI2536, Plk1-specific inhibitor, was used to understand the influence of Plk1. Also, the embryos were assessed by immunofluorescence. All BI2536-treated embryos failed to the first mitotic division. It showed Plk1 has a critical role in the first mitotic division of the mouse embryo. Moreover, there were significant differences between the control and SCNT embryos in the patterns of Plk1. All SCNT embryos which failed 2-cell development presented incorrect positioning and low expression of Plk1. On the other hand, the control embryos which failed to 2-cell division showed only low expression of Plk1. Taken together, this results demonstrate that Plk1 is critical for successful mitotic division of mouse embryos. Also, correct localization of Plk1 has crucial effect in the development of murine SCNT embryos.
( Jeonghyeon Im ),( Hee Young Kwon ),( In Kyoung Kim ),( Chang Dong Yeo ),( Sei Won Kim ),( Heayon Lee ),( Jung Won Heo ),( Sang Haak Lee ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-
Purpose Hyperbaric oxygen therapy (HBO) to increase anticancer effect, despite several positive Results, has been suggested to have a toxicity problem in other tissues, so further studies on the conditions and application of oxygen therapy are needed. The purpose of this study is to evaluate the anticancer effect of paclitaxel (PTX) in lung cancer cells under normobaric oxygen exposure. Methods Non-small cell lung cancer (NSCLC) cell line A549 and the paclitaxel resistant cell line A549/PR were used. Cell viability, ability of migration and proliferation were evaluated in room air (RA) or normobaric oxygen (NBO) air condition for 48hs. The expression apoptosis related proteins was detected. For elucidation of the cell death in PTX treated A549/PR cells by NBO, the expression level of the C/EBP homologous protein (CHOP) and autophagy related proteins were analyzed. Results NBO exposure attenuated cell viability, ability of migration and proliferation ability in A549 cells and A549/PR cells therby increased apoptotic cell death by PTX. CHOP expression of A549/PR cells was increased by NBO exposure compare to RA condition. And conversion of LC3B-I to LC3B-II by PTX was reduced in A549 and A549/PR cells in NBO exposure (Figure 1-3). Conclusions Our Results show that NBO exposure synergistically increased the apoptotic cell death of A549 and A549/PR cells by paclitaxel. Also, cell death of A549/PR cells under NBO condition was induced by CHOP-mediated disregulation of autophagy. These findings suggest that the NBO exposure can promote the sensitivity of paclitaxel in lung cancer cells by CHOP activation and disregulation of autophagy.
Pharmacometabolomic Approach to Predict QT Prolongation in Guinea Pigs
( Jeonghyeon Park ),( Keumhan Noh ),( Hae Won Lee ),( Mi Sun Lim ),( Sook Jin Seong ),( Jeong Ju Seo ),( Eun Jung Kim ),( Wonku Kang ),( Young Ran Yoon ) 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0
Drug-induced torsades de pointes (TdP), a life-threatening arrhythmia associated with prolongation of the QT interval, has been a significant reason for withdrawal of several medicines from the market. Prolongation of the QT interval is considered as the best biomarker for predicting the torsadogenic risk of a new chemical entity. Because of the difficulty assessing the risk for TdP during drug development, we evaluated the metabolic phenotype for predicting QT prolongation induced by sparfloxacin, and elucidated the metabolic pathway related to the QT prolongation. We performed electrocardiography analysis and liquid chromatography-mass spectroscopy-based metabolic profiling of plasma samples obtained from 15 guinea pigs after administration of sparfloxacin at doses of 33.3, 100, and 300 mg/kg. Principal component analysis and partial least squares modelling were conducted to select the metabolites that substantially contributed to the prediction of QT prolongation. QTc increased significantly with increasing dose (r = 0.93). From the PLS analysis, the key metabolites that showed the highest variable importance in the projection values (>1.5) were selected, identified, and used to determine the metabolic network. In particular, cytidine-5`-diphosphate (CDP), deoxycorticosterone, L-aspartic acid and stearic acid were found to be final metabolomic phenotypes for the prediction of QT prolongation. Metabolomic phenotypes for predicting drug-induced QT prolongation of sparfloxacin were developed and can be applied to cardiac toxicity screening of other drugs. In addition, this integrative pharmacometabolomic approach would serve as a good tool for predicting pharmacodynamic or toxicological effects caused by changes in dose.