Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

응급실에 내원한 환자에서 어지럼증의 원인과 임상양상의 분석

문원식,정경운,위준선,문정미,전병조,김용권,소정일,류진호,허탁,민용일 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Background: Recently, The number of patients who have been complaining of the vertigo or dizziness has been increasing due to rapid growth of elderly population and senile disease. The differentiation of dizzy patients is not familiar to most emergency physicians. This study was designed to differentiate true vertigo and to investigate the clinical difference among central vertigo, peripheral vertigo, and other causes of dizziness. Methods: The authors analyzed the cases of 237 dizzy patients who visited the emergency department of Chonnam University Hospital during the recent 2 years. For the base of dizziness, associated past illnesses, severity, nystagmus type, and the causes of central and peripheral vertigo. Results: Female patients were 142(59.9%) and male patients were 95(40.1%). The most common age group was the 6th decade. The origin of dizziness was classified as peripheral cause(25.3%), central cause(32.9%), and others(41.8%). As to nature of the dizziness, the rotatory sense was dominant in peripheral vertigo and the floating sense and blurred vision in central vertigo. Peripheral vertigo was triggered by position change of head and body, and central vertigo by the eye movement. Audiograms I showed that most patients with central vertigo had normal hearing, but 46.7% of thoswith peripheral vertigo had an abnormal hearing disturbance. Nystagmus was more prevalent in peripheral vertigo Conclusion: History taking and physical examination played an important role in the diagnosis of dizzy patients. An important part of the diagnosis of a dizzy patient could be to evaluate the peripheral origin, the central origin and others causes. Because central vertigo may be associated with a significant neurological pathology, spectral radiological studies, including MRI and CT, are mandatory to rule out a devastating brain lesion.

흰쥐에서 말초신경 손상후 뒤뿌리신경절세포의 신경영양인자와 그 수용체의 발현 양상

백선용,양만석,문총석,김봉선,김재봉,김진정 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.2

감과실에서 분리한 효모의 특성

정용진,김주현,이명희 東國專門大學 1997 金龜論叢 Vol.4 No.1

감과실에서 분리하여 탄닌물질에 내성이 있고 알콜발효력이 우수한 Saccharomyces cerevisiae YJK 20과 Saccharomyces kluyveri DJ 97의 배양학적 특성을 조사하였다. 그 결과 배양은도 30℃ YJK 20, DJ 97은 알콜수율이 95.62%, 94.43%로 가장 높았으며, 초기 pH의 영향은 YJK 20은 pH 6.0에서 DJ 97은 pH 5.0에서 각각 95.76%, 95.23%로 가장 높았다. 그리고 진탕속도의 영향은 두균주 모두 150rpm 이상에서 수율이 감소하는 경향이었다. 또한 초기 당농도에 의한 영향에서는 YJK 20은 150g/ℓ의 농도에서 97.55%, DJ 97은 100g/ℓ의 농도에서 96.55%로 가장 높은 수율을 나타내었다. 따라서 두균주는 식초의 기질이 되는 감알콜발효의 수율을 높일 수 있는 우수한 균주로 선별할 수 있었다.

Bromelain과 Zn-Methlonnine 혼합 급여가 젖소의 산유량 및 체세포수에 미치는 영향

정유진,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

Holstein cows(n=8) were assigned to control diet(n=4) and treatment diet(n=4) containing products of Bromelain(50g/kg) and Zn-methionine (133g/kg). Basal diet was mixed as total mixed rations with 60% concentrate and 40% roughage(rice straw) and fed for 8 weeks. The milk production, somatic cell counts in milk were measured and determined. The results were summarized as follow. Average milk production was higher for cows fed treatment diet(30.2kg/d) than cows fed control diet(29.6kg/d) (P<0.05). The somatic cell counts was significantly lower for cows fed treatment diet(179.8×10³/ml) than cows fed control diet (260.8x10³/ml)(P<0.05). In conclusion, supplementation of both Bromelain and Zn-methionine increased milk production and reduced somatic cell counts in milk. 본 실험은 사료 첨가제로써 Bromelain과 Zn-methionine의 급여가 착유우의 산유량 및 체세포 수 함량에 미치는 영향을 규명하고자 실시하였다. 홀스타인 착유우 8두(대조구 4두 및 처리구 4두)를 공시하여 대조구는 기본 사료를 급여하고 처리구에서는 Zn-methionine 133g/kg(Zn 20 및 methionine 46.7g)과 bromelain 50g/kg(br 30g/kg)을 경구투여하였다. 그리고 시험은 착유우의 비유초기에 8주간 실시하였다. 본 시험에서 얻은 결과는 다음과 같다. 두당 평균 유량은 대조구(29.6kg)에 비하여 처리구(30.2kg)에서 높게 나타났다(P<0.05). 우유내 체세포수는 대조구(260.8Xl0³ml)에 비하여 처리구(179.8X10³ml)에서 현저하게 낮게 나타났다(P<0.05). 따라서 이들 첨가물의 급여는 착유우의 산유량을 증가시키고 우유의 위생상 지표가 될 수 있는 체세포수의 감소를 가져오는 결과를 얻었다.

시판 및 속성 감식초의 이화학적 특성

정용진,서권일,김광수 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.3

Physicochemical properties of marketing and intensive persimmon vinegar were investigated, the results are as following: Total. acidity in D, H, S, J, sweet persimmon(SP) and persimmon vinegar were 4.20, 3.03. 2.58, 3.30, 5.92 and 5.81%, respectively, and the value of intensive persimmon(IP) vinegars were higher than that of others. SP sample was lower than any other vinegar as compared to L value in colors, no sample was lower than H and S vinegar in turbidity and browning. Brix of IP samples were lower than that of other vinegars, there was a little fructose in the all samples. Among the organic acids, galacturonic acid content except acetic acid was the highest, and ascorbic and malic acid were detected a little. Calium content was the highest of minerals in persimmon vinegars, phosphorus was detected a little. Alcohols of 5~6 types were analyzed in each samples, free amino acids of 17~24 types were analyzed, D and IP vinegars have more alcohols and amino acids, and their contents were higher than the others. Volatile components of 6~9 types were analyzed in each samples, these have more ethanols except acetic acids than other volatile components, and the contents of IP samples were higher than that of marketing persimmon vinegars.

대구·경북지역의 전통발효식품 이용에 관한 실태조사

정용진,김주현 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.3

This survey was carried out to investigate the usage of traditional fermented foods. Questionaries were answered by 283 female adults ranging from 20 to 60 ages in Daegu city and Kyungbuk province. 39.72% of total subjects answered that they made Meju and soybean paste by themselves. There were significant differences according to income, residential area, education level, and family size. In the results of the preference and consumption study on traditional soybean pastes, Kimchi and salted sea foods, preference of salted sea foods was the highest, whereas the consumption of salted sea foods was the lowest. The recognition of soybean pastes and traditional foods was remarkably low. However, the consumption frequency of traditional fermented foods and the recognition of soybean pastes and traditional foods were significantly increased with age.

감과실 알코올 발효를 위한 효모의 분리

정용진,서권일,신승렬,서지형,강미정,김광수 동아시아식생활학회 1997 동아시아식생활학회지 Vol.7 No.4

To utilize abundant poor persimmon fruits effectively, it was studied on alcohol fermentation strains which were isolated and indentified from poor persimmon fruits. The strains which had exellent alcohol production ability were analysized morphogical, cultural characteristics and identified Saccharomyces cerevisiae YJK 20, Sacahroimyces kluveri DJ 97. Alcohol production ability between the isolated strains and industrial alcohol yeasts was compared in the YPD medium, persimmon extract medium. Isolated strains had high alcohol production ability in both of two medium, respectively. So it was expected that the strains, YKJ 20 and DJ 97 had good alcohol fermentation ability of persimmon fruits.

소화성 궤양 환자에 대한Lansoprazole의 치료 효과

정현용,김진희 최신의학사 1997 最新醫學 Vol.40 No.6