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젖소 유방염 유래 Staphylococcus aureus의 PCR을 이용한 Genomic Fingerprinting
김두,권순탁,안소저,Kim, Doo,Kwon, Soon-Tak,An, So-Jeo 한국임상수의학회 1999 한국임상수의학회지 Vol.16 No.1
A total of 137 strains of Staphylococcus aureus were isolated from dairy cow's milk with subclinical mastitis from 33 herds in 5 provinces and 36 strains of S aureus from clinical mastitis from 4 herds where the mastitis were severe problem. Arbitrary primed polymerase chain reactions with 10 bp oligonucleotide primer were performed and the PCR products were analysed with image analyzer, The S aureus strains were genotyped into 20 distinct DNA fingerprinting profiles. The size of PCR products ranged from 163 to 2,479 bp and PCR products of 506, 770, 784 and 2,479 bp were the most prevailing bands. Genotype 3 was founded in all 5 provinces. The various genotypes were identified in newly founded dairy herds, however, only one or two genotypes were identified in the closed herds. In clinical mastitis, only a limited number of different S aureus genotype was founded in each of the herds in comparision with subclinical mastitis. The results demonstrated that PCR-based DNA fingerprinting analysis of S aureus strain can be used to study epidemiology of mastitis, in addition, common genotype in geographic region can be useful for the development of an effective S aureus bacterin.
꼬마배나무이 (Cacopsylla pyricola) 저항성 배 육종재료 탐색
신일섭 ( Il Sheob Shin ),김동순 ( Dong Soon Kim ),홍성식 ( Seong Sik Hong ),김정희 ( Jeong Hee Kim ),조강희 ( Kang Hee Cho ),김세희 ( Se Hee Kim ),김현란 ( Hyun Ran Kim ),김대현 ( Dae Hyun Kim ),홍세진 ( Se Jin Hong ),황정환 ( Jeo 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.5
Breeding for pear resistance to pear psylla (Cacopsylla pyricola) is one of important objective of the National Institute of Horticultural and Herbal Science breeding program. One hundred thirty three accessions from 15 Asian, Chinese and European pear species were investigated for their resistance against pear psylla. The pear psylla resistance was determined based on the following four characteristics: overwintering adult population, the number of eggs and nymphs, and the degree of soot. The different pear species showed varied resistance to pear psylla. Pyrus calleryana and P. betulaefolia indicated the highest antixenosis as ovipositional preference and antibiosis as nymphal feeding and were the most resistant genetic resources. Likewise the European pears (P. communis), ``Conference`` and ``Cascade``, exhibited little occurrence and damage by pear psylla. These were proved to be promising genetic materials for breeding resistant cultivars because they had good fruit quality and showed resistance to pear paylla. The observed population of overwintering adult, the number of eggs and nymphs of psylla had significant correlation each other.
Faraday효과를 이용한 광CT의 온도특성에 관한 연구
전재일,허순영,박원주,이광식,김정배,김민수,Jeon, Jeo-Il,Heo, Soon-Young,Park, Won-Zoo,Lee, Kwang-Sik,Kim, Jung-Bae,Kim, Min-Soo 한국조명전기설비학회 2005 조명·전기설비학회논문지 Vol.19 No.1
본 논문에서는 패러데이 효과(Faraday effect)를 이용한 초고압 전력설비에서의 대전류 측정을 위한 광CT의 온도 특성에 대한 기초연구를 기술하였다. 1310[nm] 레이저 다이오드를 광원으로 사용하고 PIN-Photodiode를 수신부로 사용하고 광의 전송로는 Fiber내에서 편광상태를 유지 할 수 있는 30[m]의 단일 모드 광섬유를 사용하였다. 온도 변환에 따른 실험을 하기 위한 온도변환장치는 알루미늄으로 제작하였다. 전류의 측정은 400[A]에서 1300[A]까지의 범위에서 측정하였으며 온도는 $-40[^{\circ}C]$에서 $50[^{\circ}C]$까지 $10[^{\circ}C]$씩 증가시키면서 측정하였다. 동일한 실험 환경하에서 출력신호는 인가전류가 증가할수록, 주변온도가 높을수록 비례하여 증가하였다. In this paper, we wrote about the basic experimentation of Optical CT's temperature characteristic to measure high-current in a super-high-voltage electric power equipment which is using Faraday effect. We used the 1310[nm] Laser Diode as the light source and PIN Photodiode as receiver. For the transmission line of light, we used 30[m] single mode fiber which could maintain the state of polarization in the optical fiber. For the experiment, the temperature transformation device make by aluminium. the The range of current was from 400[A] and 1300[A] and the range of temperature was from $-40[^{\circ}C]\;to\;50[^{\circ}C]$. In a same experimental condition, magnitude increased input current increase follow by increasing proportion of input current.
CP와 TDN 수준이 육성기 유산양의 건물섭취량, 소화율과 질소 및 에너지 이용성에 미치는 영향
기광석(Kwang Seok Ki),임영순(Young Soon Lim),김택림(Ze Lin Jin),이현준(Hyun June Lee),김상범(Sang Bum Kim),이왕식(Wang Shik Lee),양승학(Seung Hak Yang),조원모(Won Mo Cho),김현섭(Hyeon Shup Kim),여준모(Joon Mo Jeo),이인덕(In Duk Lee) 韓國草地學會 2009 한국초지조사료학회지 Vol.29 No.3
유산양의 에너지 및 단백질 이용성을 조사하기 위하여 대조구(A), TDN 증량구(B), 조단백질 증량구(C) 그리고 TDN 및 조단백질 증량구(D)로 구분하여 섭취량, 소화율과 질소 및 에너지 이용성을 시험하였다. 공시축은 유산양(Saanen, 평균체중 17.5㎏, ♀) 12두를 공시하였고, 2008년 10월 20일부터 11월 9일까지 충남대학교 부설동물사육장에서 수행하였다. 건물섭취량은 D구에서 가장 높았으며 B구가 C구보다 유의적으로 높은 섭취율을 보였다(p<0.05). 건물 소화율 및 세포내용물의 소화율은 A구에 비하여 영양성분을 증량한 B구와 C구 및 D구가 모두 유의적으로 높은 결과를 보였으며(p<0.05), D구가 가장 높고 B구가 C구보다 높은 수준을 보였다. NDF 소화율은 A구에 비하여 영양성분을 증량한 처리구들에서 모두 높았고, ADF 소화율은 A구와 C구가 B구와 D구보다 다소 높은 결과를 보였는데 유의적인 차이는 없었다. 가소화 및 대사질소량은 섭취량과 유사한 경향으로 D구에서 가장 높았으며, C구 보다는 B구가 유의적으로 높은 수준을 보였다(p<0.05). 가소화에너지와 대사에너지도 D구에서 가장 높았으며 C구 보다는 B구가 유의적으로 높은 수준을 보였다(p<0.05). 이상의 결과들은 조단백질만을 증량하는 것보다는 TDN 수준과 함께 조단백질수준을 증량시켜주거나 또는 TDN 수준을 증량시키는 것이 건물소화율 및 질소이용성과 에너지이용성을 향상시키는데 더 효과적이라는 결과를 보였다. This experiment was carried out to investigate the effects of increases of nutrient level of TMR on dry matter intake, digestibility, nitrogen and energy balance in growing dairy goats (Saanen). Twelve growing dairy goats weighing 17.5㎏ were randomly assigned to one of four TMRs; low energy-low crude protein (CP) TMR (control; A), high energy-low CP TMR (B), low energy-high CP TMR (C) and high energy-high CP TMR (D). The content of total digestible nutrients (TDN) and CP in the control diet were 67% and 11%. The TDN content of the high energy TMR was 73.7% and the CP content of the high CP TMR was 13%. Dry matter intake was highest in D, and significantly higher in B than in C (p<0.05). Digestibility for dry matter and cell contents increased in proportion to dry matter intake. NDF digestibility was higher in D than in A, while ADF digestibility was higher in A and C than in B, but was not significant. Digestible nitrogen, apparently digested nitrogen and retained nitrogen were correlated with intake, and significantly higher in B than in C (p<0.05). Digestible energy and metabolizable energy were highest in D, and significantly higher in B than in C (p<0.05). Therefore, the present results showed that D or B were better than C for high intake, digestibility, nitrogen and energy utilization.
Primary Ureteral Transitional Cell Carcinoma in a Dog
김성수,이저순,윤수경,김수연,오현정,손정민,정선영,김보은,지서연,김대용,김완희,윤정희,최민철,Kim, Seong-soo,Lee, Jeo-soon,Yun, Soo-kyung,Kim, Su-yeon,Oh, Hyun-jung,Sohn, Jung-min,Jung, Sun-young,Kim, Bo-eun,Ji, Seo-yeoun,Kim, Dae-yong,Kim, W The Korean Society of Veterinary Clinics 2015 한국임상수의학회지 Vol.7 No.2
14살령의 중성화 수컷 개가 혈뇨와 배뇨통으로 내원하였다. 복부초음파 검사에서 편측성 수신증과 요관 벽이 국소적으로 두꺼워진 수뇨관증이 진단되었다. 수술적 탐색으로 좌측 근위 요관에서 요관 유래의 관내 종괴가 확인되었으며, 종괴 절제술이 실시되었다. 요관 종괴는 조직학적 검사를 통해 유두상 이행세포암종으로 진단되었다. 환자는 안정적으로 회복되었으나, 수술 3개월 후 또 다른 종괴가 우측 신장에서 발견되었다. 신장 종괴에 대한 세포학적 검사 결과 간엽세포종양으로 진단되었다. A 14-year-old, castrated male mixed-breed dog weighing 9 kg was presented for hematuria and dysuria. Abdominal ultrasound showed unilateral hydronephrosis and hydroureter with focal thickening of the ureteral wall. Surgical exploration revealed an intraluminal mass arising from the proximal left ureter. Mass resection was performed. Histopathology of the ureteral mass was consistent with a papillary transitional cell carcinoma. The patient recovered well post-operatively, but was diagnosed with another tumor three months later, this time in the right kidney. Fine-needle aspiration cytology of the renal mass revealed an epithelial cell tumor with mesenchymal features.
Imaging Findings of Pneumothorax Caused by Bronchial Cartilage Hypoplasia in a Dog
김수연,김성수,이저순,윤수경,오현정,손정민,김보은,김완희,윤정희,최민철,Kim, Su-yeon,Kim, Seong-soo,Lee, Jeo-soon,Yun, Soo-kyung,Oh, Hyun-jung,Shon, Jung-min,Kim, Bo-eun,Kim, Wan-hee,Yoon, Jung-hee,Choi, Min-cheol The Korean Society of Veterinary Clinics 2015 한국임상수의학회지 Vol.7 No.2
10년령의 중성화된 수컷 Poodle이 기흉이 의심되어 내원하였다. 어릴 때부터 기침증상을 보이다가 약 2주 전부터 악화되었으며, 혈액검사 및 요검사에서 유의적인 특이소견은 발견되지 않았다. 흉부 방사선 영상에서 기흉 소견 및 좌측 전엽 부위 기관지의 단절과 고도로 감소된 폐 실질이 관찰되었다. 흉부의 CT 영상 검사에서, 좌측 전엽과 덧엽에서 몇 개의 cyst들과 허탈된 폐가 관찰되었다. 또한 이 부위에서 기관지와 세기관지들은 비정상적인 형태로 끝부분이 가늘어지지 않았으며, 그 외에도 기흉, 기종격, 그리고 피하 기종이 확인되었다. 이러한 영상학적 특징들로부터 기종성 수포의 파열에 의해 발생한 자발성 기흉이 고려되었으며, 그 원인으로는 선천적인 기관지의 이상 또는 기관지 가지의 기형이 고려되었다. 부분적인 폐 덧엽 절제술이 실시되었으며, 좌측 전엽, 우측 중엽, 그리고 덧엽의 저형성이 확인되었고 특히 덧엽에서는 공기가 새고 있는 수포가 확인되었다. 조직병리학적 검사를 통해 기관지 연골 저형성으로 최종 진단되었다. A 10-year-old, castrated poodle dog presented with a cough for 2 weeks, and the cough initially developed since very young age. On radiographs, pneumothorax was noticed by characteristics of radiolucent area without pulmonary markings along the thoracic wall and diaphragm, retracted lung lobes from the thoracic wall and severely decreased volume of the left cranial lung lobe with disconnected bronchus. Computed tomography (CT) findings identified several pulmonary air-filled cysts and collapsed lung with abnormal shape and non-tapered end of bronchus, bronchioles at the accessory lobe and left cranial lobe. Also, pneumothorax, pneumomediastinum and subcutaneous emphysema were found. Imaging diagnosis was the spontaneous pneumothorax caused by ruptured emphysematous bullae associated with congenital bronchial cartilage abnormality or bronchial tree malformation. On surgery, hypoplasia of the left cranial lobe, right middle lobe, and accessory lobe with a bulla where air was leaking was identified. The accessory lobe was partially resected and bronchial cartilage hypoplasia was confirmed by histopathologic examination.
후천성면역결핍증후군 환자에게 급성 호산구성 폐렴 양상을 보인 폐포자충 폐렴
이보라 ( Bo Ra Lee ),김현국 ( Hyun Kook Kim ),박이내 ( I Nae Park ),최상봉 ( Sang Bong Choi ),정훈 ( Hoon Jung ),이현경 ( Hyun Kyung Lee ),이성순 ( Sung Soon Lee ),이영민 ( Young Min Lee ),이혁표 ( Hyuk Pyo Lee ),최수전 ( Soo Jeo 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.2
A 73-year-old man was admitted with a sudden onset of dyspnea. He had never smoked. The chest radiograph and computed tomography revealed bilateral ground glass opacity and an enlarging perihilar consolidation with lymphadenopathies. There was a higher percentage of eosinophils (72%) in the bronchoalveolar lavage fluid (BALF) than normal. The patient was diagnosed with acute eosinophilic pneumonia and managed with steroid. Pneumocystis pneumonia (PCP) was diagnosed by an examination of the BALF, and the patient was treated with trimethoprim-sulphamethoxazole. The patient tested positive to the HIV antibody and the peripheral blood CD-4 positive lymphocyte count was only 33/μL. The percentage of eosinophils in the BALF can increase in some cases of PCP that is complicated with AIDS. Only a few cases of eosinophilic pneumonia associated with PCP pneumonia have been reported in patients with AIDS but there are no case reports in Korea. This case highlights the need to consider PCP when the percentage of eosinophils in the BALF is elevated.