베타카로틴 함유 마이크로에멀젼의 제조 및 물리화학적 특성 평가

곽은선,이종화,박목순,이계원,지웅길 충남대학교 약학대학 의약품개발연구소 2000 藥學論文集 Vol.16 No.-

The o/w microemulsions containing β-carotene were made from 3 or 5% w/w oil(soybean oil, cottonseed oil and isopropyl myristate) and 15, 20 or 25% w/w nonionic surfactant (Cremophor EL, Cremophor RH 40 and Brij 97/Pluronic F-68(2:1 w/w). They are characterized by particle size distribution, surface tension and viscosity. Also, stability study was done by antioxidant, long-term storage for 6 months.

Genotypes of Alcohol - Metabolizing Enzymes and the Risk for Alcoholics in Korean

Mok, Jee Won,Park, Kyung Sook,Seol, Hye Won 한국유전학회 1997 Genes & Genomics Vol.19 No.4

Alcohol dehydrogenase 2 (ADH2) and mitochondrial aldehyde dehydrogenase 2 (ALDH2) are the major enzymes involved in oxidative degradation of ethanol, and they catalyze the conversion of ethanol to acetaldehyde and further into acetic acid. The frequencies of ADH2^*2 and ALDH2^*2 varied across ethnic groups and the distortion frequencies were shown in alcoholics. We examined the exon 3 of the ADH2 and exon 12 of the ALDH2 genotypes by PCR-RFLP from 53 alcoholics and 232 controls in Korean. The ADH2^*1/ADH2^*1 homozygote was significantly predominant in alcoholic patients (45.3%) compared to control subjects (6.0%) (p<0.001), whereas there was inverse relation in ADH2^*2/ADH2^*2 (p<0.001). The ALDH2^* 1/ALDH2^* 1 homozygote was 94.3% for alcoholics compared with 75.9% of controls (p<0.001). All alcoholics had AL DH2^* 1 allele in our study. The high relative risks for alcoholics were found in ALDH2^* 1 and ALDH2^* 1 and their relative risks (R.R.) are 2.9 and 8.1, respectively. On the other hands, the allele frequencies of ADH2^* 2 and ALDH2^* 2 for alcoholics were lower than those of controls. These data suggest that ADH2 and ALDH2 gene polymorphisms may be associated with tloe development of alcoholics in Korean. Same genetic predisposition to alcoholics was shown in other Orientals (Japanese and Chinese) but not in Caucasians.

Association with Corneal Remodeling Related Genes, ALDH3A1, LOX, and SPARC Genes Variations in Korean Keratoconus Patients

Jee-Won Mok,Ha-Rim So,Min-Ji Ha,Kyung-Sun Na,Choun-Ki Joo 대한안과학회 2021 Korean Journal of Ophthalmology Vol.35 No.2

Purpose: To determine whether the cornea remodeling-related genes aldehyde dehydrogenase 3A1 (ALDH3A1), lysyl oxidase(LOX), and secreted protein acidic and rich in cysteine (SPARC) were potential susceptibility candidate genes for keratoconusin Korean patients, we investigated the associations of single nucleotide polymorphisms (SNPs) in these three genes in Koreanpatients with keratoconus. Methods: Genomic DNA was extracted from blood samples of unrelated patients with keratoconus and healthy control individuals. For screening of genetic variations, all exons from the entire coding regions of the ALDH3A1, LOX, and SPARC geneswere directly sequenced to determine the presence of mutations. Control individuals were selected from the general populationwithout keratoconus. Results: In this study, we detected nine SNPs in ALDH3A1, four SNPs in LOX, and 18 SNPs in SPARC. rs116992290, IVS3-62c>t,rs116962241, and rs2228100 in ALDH3A1 and rs2956540 and rs1800449 in LOX were significantly different between patientand control groups. In the SPARC gene, the distribution of the *G allele of EX10+225 T>G (p = 0.018; odds ratio, 1.869) wasstrongly associated with the risk of keratoconus in the Korean population. In haplotype analysis, C-G of rs2956540-rs2288393in LOX (p = 0.046) and C-C-G and G-G-G of rs60610024-rs2228100-rs57555435 (p = 0.021 and p < 0.001), G-A of IVS3-62 a>g- rs116962241 in ALDH3A1 (p = 0.048) predisposed significantly to keratoconus. After cross-validation consistency and permutationtests, two locus model was the best SNP variations interaction pattern. Conclusions: Our results suggested that genetic variations in ALDH3A1, LOX, and SPARC genes were associated with a predispositionfor keratoconus in Korean individuals. Moreover, variations in ALDH3A1and LOX may serve as strong biomarkers forkeratoconus.

5- Fluorouracil 과 그 유도체를 봉입한 Multilamellar Vesicle(MLV)과 Microemulsified Liposome(MEL의 특성 및 약물방출 거동

지웅길,이계원,박목순,류연근 한국약제학회 1995 Journal of Pharmaceutical Investigation Vol.25 No.3

Although liposome has many advantages as a pharmaceutical dosage form, its application in the industrial field has been limited because of some problems such as preparation method, reproducibility, scale-up, stability and sterilization etc. Liposomes prepared by microemulsification method had defined size, narrow size distribution, reproducibility and high entrapment efficiency. For enhancing the stability, the dry form of liposome was recommended. These types of liposome are proliposome and freeze-dried liposome. The liposome must have some properties for preparing of freeze-dried liposome: small size (50∼200nm), narrow size distribution and cryoprotectant. In this experiment, the liposomes containing 5-Fluorouracil(5-FU) and its prodrug (pentyl-5-FU-1-acetate: PFA, hexyl-5-FU-1-acetate: HFA) were made with soybean phosphatidylcholine, cholesterol, stearylamine(SA) and dicetyl phosphate(DCP) employing hydration method or microemulsification method using Microfluidizer^(TM). Both of liposome types were MLV and MEL. After preparation, freeze drying and rehydration were performed. In the process of freezing, trehalose(Tr) was added as a cryoprotectant. Their evaluation methods were as follows: entrapment efficiency, mean particle size and size distribution, dissolution test, retain of entrapment efficiency and turbidity after freeze-drying. The results are summarized as belows. The entrapment efficiency of 5-FU was dependent on total lipid concentration and cholesterol content but that of PFA and HFA was decreased when cholesterol was added. When DCP and SA were added, entrapment efficiency was decreased. As the partition coefficient of drug was increased, entrapment efficiency was increased. Under the same condition, entrapment efficiency of MEL is similar to that of MLV. The mean particle size and size distribution of MEL were smaller than those of MLV. Dissolution rates of drug from both liposome types were comparatively similar. Dissolution rates of drugs with serum and liver homogenate were faster than without these materials. Alter preparation of liposome, free drug was removed effectively by Dowex 50W-X4. When liposome was freeze-dried and then rehydrated in the presence of Tr, characteristics of liposome were maintained well in MEL than MLV. Tr was used successfully as a cryoprotectant in the process of freeze drying and the optimal ratio of Tr:Lipid was 4:1(g/g).

5-Fluorouracil과 그 유도체를 봉입한 Multilamellar Vesicle(MLV)과 Microemulsified Liposome(MEL)의 특성 및 약물방출 거동

지웅길,박목순,이계원,류연근 충남대학교 약학대학 의약품개발연구소 1995 藥學論文集 Vol.11 No.-

Although liposome has many advantages as a pharmaceutical dosage form, its application in the industrial field has been limited because of some problems such as preparation method, reproducibility, scale-up, stability and sterilization etc. Liposomes prepared by microemulsification method had defined size, narrow size distribution, reproducibility and high entrapment efficiency. For enhancing the stability, the dry form of liposome was recommended. These types of liposome are proliposome and freeze-dried liposome. The liposome must have some properties for preparing of freeze-dried liposome; small size(50~200nm), narrow size distribution and cryoprotectant. In this experiment, the liposomes containing 5-Fluorouracil(5-FU) and its prodrug (pentyl-5-FU-1-acetate: PFA, hexyl-5-FU-l-acetate: HFA) were made with soybean phosphatidylcholine, cholesterol, stearylamine(SA) and dicetyl phosphate(DCP) employing hydration method or microemulsification method using Microfluidizer^TM. Both of liposome types were MLV and MEL. After preparation, freeze dying and rehydration were performed. In the process of freezing, trehalose(Tr) was added as a cryoprotectant. Their evaluation methods were as follows; entrapment efficiency, mean particle size and size distribution, dissolution test, retain of entrapment efficiency and turbidity after freeze-drying. The results are summarized as bellows. The entrapment efficiency of 5-FU was dependent on total lipid concentration and cholesterol content but that of PFA and HFA was decreased when cholesterol was added. When DCP and SA were added, entrapment efficiency was decreased. As the partition coefficient of drug was increased, entrapment efficiency was increased. Under the same condition, entrapment efficiency of MEL is similar to that of MLV. The mean particle size and size distribution of MEL were smaller than those of MLV. Dissolution rates of drug from both liposome types were comparatively similar. Dissolution rates of drugs with serum and liver homogenate were faster than without these materials. After preparation of liposome, free drug was removed effectively by Dowex 50W-X4. When liposome was freeze-dried and then rehydrated in the presence of Tr. Characteristics of liposome were maintained well in MEL than MLV. Tr was used successfully as a cryoprotectant in the process of freeze drying and the optimal ratio of Tr:Lipid was 4:1(g/g).

An Overmodulation Method for Space Vector PWM Inverters with DC-Link Shunt Resistor

Jee-Sang Lee,Won-Sang Im,Jang-Mok Kim 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5

In order to lower the cost and expand the working range of pulse width modulation voltage source inverter (PWM-VSI), the control method under the overmodulation mode with a dc-link shunt resistor is developed. The voltage utilization according to un-implementable region is analyzed in overmodulation region. By using the conventional phase reconstruction method, the minimized un-implementable region can be achieved for maximizing voltage utilization and the minimizing total harmonic distortion (THD) under the overmodulation region. The new overmodulation voltage reference which has the same voltage utilization with original voltage reference is produced to minimize THD by using Fourier analysis. The effectiveness of proposed algorithm is verified through the THD comparison between conventional and proposed method. The experimental results verify the validity of new overmodulation control and behavior of phase current reconstruction method.

개에서 Chiari-like malformation에 의한 척수공동수증과 수두증의 발생 1례

신영지,박정희,김영실,최호정,이영원,정성목 忠南大學校 獸醫科大學 動物醫科學硏究所 2005 動物醫科學硏究誌 Vol.13 No.-

A 2.5㎏, 11-year old, intact female mixed breed dog with 1 year history of sporadic seizure was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. There were no significant finding in CBC and serum chemistry. Based on X-ray, CT scan and MRI, it was diagnosed as syringohydromyelia and hydrocephalus caused by Chiari-like mlaformation. Prednisolone, furosemide and phenobarbital was administered orally. Depend on the number and severity of seizure, the dose of phenobarbital was adjusted. Seizure episode was reduced.

Development of a Immuno-Chip for Simultaneous Detection of Genetically Modified Organisms

Hye-Jee Jang(장혜지),Eui-Hwan Paek(백의환),Won-Mok Park(박원목),Se-Hwan Paek(백세환) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.10

Development of a Chemiluminometric Immunosensor Array for Genetically Modified Organisms (GMOs)

Hye-Jee Jang,Eui-Hwan Paek,Won-Mok Park,Se-Hwan Paek 한국생물공학회 2005 한국생물공학회 학술대회 Vol.2005 No.4

Midfacial degloving approach for management of the maxillary fibrous dysplasia: a case report

Kang, Miju,Jee, Yu-jin,Lee, Deok won,Jung, Sang-pil,Kim, Se-won,Yang, Sunin,Ryu, Dong-mok Korean Association of Maxillofacial Plastic and Re 2018 Maxillofacial Plastic Reconstructive Surgery Vol.40 No.-

Background: Fibrous dysplasia (FD) is a benign bone lesion characterized by the progressive replacement of normal bone with fibro-osseous connective tissue. The maxilla is the most commonly affected area of facial bone, resulting in facial asymmetry and functional disorders. Surgery is an effective management option and involves removing the diseased bone via an intraoral approach: conservative bone shaving or radical excision and reconstruction. Case presentation: This case report describes a monostotic fibrous dysplasia in which the patient's right midface had a prominent appearance. The asymmetric maxillary area was surgically recontoured via the midfacial degloving approach under general anesthesia. Follow-up photography and radiographic imaging after surgery showed the structures were in a stable state without recurrence of the FD lesion. Furthermore, there were no visible scars or functional disability, and the patient reported no postoperative discomfort. Conclusions: In conclusion, the midfacial degloving approach for treatment of maxillary fibrous dysplasia is a reliable and successful treatment option. Without visible scars and virtually free of postoperative functional disability, this approach offers good exposure of the middle third of the face for treatment of maxillary fibrous dysplasia with excellent cosmetic outcomes.