Comparative Epigenetics in Companion Dogs

Je-Yoel Cho(조제열) 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.1

소(우(牛))의 식도구 평활근 절편에 대한 catecholamine의 작용

조제열,양일석,Cho, Je-yoel,Yang, Il-suk 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.2

Effects of catecholamines were investigated on isolated strips of the male cattle oesophageal groove. In the circular muscles of the bottom and longitudinal muscles of the lip. isometric tensions was recorded with isometric myograph in 25ml organ bath. The results were as follows: 1. The muscular activity was different in preparations from the two parts. In the longitudinal muscle from the lip, rhythmic contractions generally occurred. while in the circular muscle from the bottom they were not seen almost. 2. In the circular muscle of the bottom, the increased tone and biphasic contractions were caused by catecholamines. And these contractions were mediated through $\alpha$-excitatory adrenoceptor. Also circular muscle showed minor inhibitory response to catecholamines. And these effects were mediated through $\beta$-inhibitory adrenoceptor. But the circular muscle was more sensitive to the $\alpha$-excitatory effect than $\beta$-inhibitory effect. 3. In logitudinal muslce of the lip. rhythmic contractions were reduced or disappeared by catecholamines(especially propranolol) and these effects were mediated through $\beta$-adrenoceptor.

소(牛) 식도구 평활근의 Adrenergic receptor 존재부위에 관한 연구

강동묵,조제열,박전홍,양일석,Kang, Tong-mook,Cho, Je-yoel,Park, Jun-hong,Yang, Il-suk 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.4

The preliminary studies on the localization of adrenoceptors were performed on smooth muscle strips of bovine esophageal groove. The mechanical activity of the muscle strip was recorded isometrically in vitro.w In the bottom circular muscle strips. the excitatory ${\alpha}-adrenergic$ responses were not blocked by tetrodotoxin$(2.1{\times}10^{-6}M)$ and denervation which was carried by cold storage of strips for 48 hrs in Tyrode's solution at $5-6{^{\circ}C}$ without oxygen supply. These excitatory ${\alpha}-adrenergic$ responses were partially blocked by atropine. In the lip longitudinal muscle strips, the inhibitory${\beta}-adrenergic$ responses were not blocked by pretreatment of tetrodotoxin and atropine. The results suggest that ${\beta}-adrenergic$ receptors mediating relaxations are located on the postsynaptic smooth muscle cells, whereas ${\beta}-adrenergic$ receptors mediating contractions are located both in the smooth muscle cells and in the cholinergic neurones.

고혈압을 동반한 만성 치주염 환자의 치은 조직에서 C-reactive protein과 macrophage colony-stimulating factor의발현

김철우,박진우,서조영,조제열,이재목,Kim, Chul-Woo,Park, Jin-Woo,Suh, Jo-Young,Cho, Je-Yoel,Lee, Jae-Mok 대한치주과학회 2009 Journal of Periodontal & Implant Science Vol.39 No.4

Purpose: The purpose of this study was to quantify and compare the expressions of CRP and M-CSF in the gingival tissues of the patients with chronic periodontitis associated to hypertension. Methods: Gingival tissue samples were obtained during periodontal surgery or tooth extraction. Clinically healthy gingival tissue samples from systemically healthy 12 patients were categorized as group 1 (n=12). Inflammatory gingival tissue samples from patients with chronic periodontitis were categorized as group 2 (n=12). Inflammatory gingival tissue samples from patients with chronic periodontitis associated with hypertension were categorized as group 3 (n=12). Tissue samples were prepared and analyzed by Western blotting. The quantification of CRP and M-CSF were performed using a densitometer and statistically analyzed by one-way ANOVA followed by Tukey test. Results: There were significant differences between group 1 and group 2 and between group 1 and group 3 in both CRP and M-CSF. The differences between group 2 and group 3 were not statistically significant in both proteins. However, the expression levels of CRP and M-CSF in hypertensive inflammatory gingiva showed increased tendency compared to non-hypertensive inflammatory gingiva. Conclusions: It is suggested that CRP and M-CSF might be used as inflammatory and bone resorption markers in periodontal diseased tissue. It is assumed that hypertension may be associated with the progression of periodontal inflammation and alveolar bone resorption.

생쥐 배아 혀 상피의 발생과정에서 나타나는 사이토케라틴 1, 14와 PAX 9에 관한 면역조직화학적 연구

정혜인(Hye-In Jung),진명욱(Myoung-Uk Jin),조제열(Je-Yoel Cho),정한성(Han-Sung Jung),김재영(Jae-Young Kim) 대한체질인류학회 2008 대한체질인류학회지 Vol.21 No.4

피부의 상피에 대한 연구는 부속기관의 발생 그리고 보호장벽 형성과 같은 다양한 분야에서 연구되어 왔다. 혀 상피조직의 발생과 관련된 보호장벽의 형성 및 상피조직 분화에 대해서는 많은 연구가 필요한 실정이다. 본 연구에서는 혀의 보호장벽이 형성되고 상피의 분화가 일어나는 발생중인 혀를 이용하여 보호장벽 형성과정을 면밀하게 관찰하였고, 피부의 상피에서 나타나는 다양한 사이토케라틴의 발현양상을 발생중인 혀에서 조직면역화학방법으로 확인하였다. 혀 상피의 발생과정에서 PAX 9의 발현이 사이토케라틴 14와 겹쳐서 나타나는 것을 확인할 수 있었으며, 또한 Ki67을 이용한 상피세포의 증식양상과 비교해 볼 때, 유사하게 나타나는 것을 확인할 수 있었는데 이는 PAX 9, 사이토케라틴 14와 세포의 증식이 혀 보호장벽 형성에 관여하는 중요한 인자로 작용할 수 있을 것이라고 생각된다. 이러한 다양한 상피분화관련 인자들의 발현양상들을 비교해 볼 때, 혀 상피의 분화는 E15에서 본격적으로 시작되어 보호장벽의 형성 이전에 사이토케라틴 14와 PAX 9과 같은 인자들의 발현이 먼저 나타나게 되면서 상피의 분화가 진행되고, 이후 사이토케라턴 1과 같은 각질화 관련 인자가 보호장벽 형성에 기여한다고 생각된다. Epithelial differentiation and morphogenesis in skin and oral mucosa were elucidated using various experimental tools. However, tongue epithelial differentiation has not been examined properly yet. In this study, we identified the relationship between morphological changes and localizations of differentiation markers, such as cytokeratins and PAX 9 in mice embryonic tongue development. Protective barrier formation and localization pattern of cytokeratins in tongue epithelium were examined with toluidine blue staining and immunohistochemistry respectively. Localization patterns of PAX 9 and Cytokeratin 14 were coincided during tongue epithelium development. In addition, compared with Ki67 localizations , marker for cell proliferation, localization patterns of PAX 9 and Cytokeratin 14 would suggest that these factors would involve in tongue barrier formation through cell proliferation. Based on these results, tongue epithelial differentiation would begin at E14 with the specific localizations of PAX 9 and Cytokeratin 14 prior to protective barrier formation then Cytokeratin 1, keratinization marker, would involve in protective barrier and filiform papillae formations.

Sodium Iodide Symporter (NIS)를 이용한 분자영상

조제열 대한핵의학회 2004 핵의학 분자영상 Vol.38 No.2

Radioiodide uptake in thyroid follicular epithelial cells, mediated by a plasma membrane transporter, sodium iodide symporter (NIS), provides a first step mechanism for thyroid cancer detection by radioiodide injection and effective radioiodide treatment for patients with invasive, recurrent, and/or metastatic thyroid cancers after total thyroidectomy. NIS gene transfer to tumor cells may significantly and specifically enhance internal radioactive accumulation of tumors following radioiodide administration, and result in better tumor control. NIS gene transfers have been successfully performed in a variety of tumor animal models by either plasmid-mediated transfection or virus (adenovirus or retrovirus)-mediated gene delivery. These animal models include nude mice xenografted with human melanoma, glioma, breast cancer or prostate cancer, rats with subcutaneous thyroid tumor implantation, as well as the rat intracranial glioma model. In these animal models, non-invasive imaging of in vivo tumors by gamma camera scintigraphy after radioiodide or technetium injection has been performed successfully, suggesting that the NIS can serve as an imaging reporter gene for gene therapy trials. In addition, the tumor killing effects of I-131, ReO4-188 and At-211 after NIS gene transfer have been demonstrated in in vitro clonogenic assays and in vivo radioiodide therapy studies, suggesting that NIS gene can also serve as a therapeutic agent when combined with radioiodide injection. Better NIS-mediated imaging and tumor treatment by radioiodide requires a more efficient and specific system of gene delivery with better retention of radioiodide in tumor. Results thus far are, however, promising, and suggest that NIS gene transfer followed by radioiodide treatment will allow non-invasive in vivo imaging to assess the outcome of gene therapy and provide a therapeutic strategy for a variety of human diseases. (Korean J Nucl Med 38(2):152-160, 2004)