수산화칼슘 처리된 Porphyromonas endodontalis Lipopolysaccharide가 다형핵백혈구의 IL-1과 TNF-α 생성에 미치는 영향에 관한 연구

박찬제,박동성,유현미,오태석,임성삼 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.5

Bacterial lipopolysaccharide (LPS) plays a major role in stimulating the synthesis and release of the principal osteoclast-activating cytokines , namely, interleukin 1 and tumor necrosis factor-α from immune cells, Although monocytes/macrophages are the main producers of these cytokines, recent evidence has indicated that polymorphonuclear leukocytes (PMN) have the ability to release IL-1 and TNF-α. Calcium hydroxide has been shown to be an effective medicament in root canal infectionsm, reducing the microbial titre within the canal. It has been proposed that the therapeutic effect of Ca(OH)_2 may also be the result of direct inactivation of LPS. The purpose of this study was to investigate whether treatment of Porphyromanas endodonatalis LPS with calcium hydroxide alters its biological action as neasured by human PMN secretion of IL-1 and TNF-α, and it was compared with Escherichia coli LPS. P.endodonatalis ATCC 35406 was cultured in anaerobic condition, and LPS was extracted using the hot-phenol water extraction method and purified. Purchased E. coli LPS was also purified . 100 ㎍/ml of each LPS in pyrogen free water were incubated with 25mg/ml Ca(OH)_2 at 37℃ for 7 days. The supernatants were subjected to ultrafiltration. And the isolates were lyophilized and weighed. PMNs were obtained from peripheral blood by centrifugation layered over Lymphoprep. The cells were resuspended (4 × 10^6 cells/ml) in RPMI 1640 followed by treatment with various concentrations of LPS (0, 0.1, 1, 10㎍/ml) for 24 hours at 37℃ in 5% CO_2 incubator. The supernatants of cells were collected and the levels of IL-1α, IL-1β and TNF-α were measured by enzyme-linked immunosorbent assay. The results were as follows; 1. The levels of IL-1α, IL-1β, TNF-α from PMN treated with each LPS were significantly higher than those released from unstimulated PMN of the control group (p<0.05). 2. The levels of all three cytokines released from PMN stimulated with each calcium hydroxide treated LPS were significantly lower than those released from PMN stimulated with each untreated LPS (p<0.05). while they were not significantly different from those released from unstimulated PMN of the control group (p>0.05). 3. The levels of secretion or all three cytokines were affected in a dose-dependent manner in PMN stimulated with each LPS (p<0.05), but not in PMN stimulated with each calcium hydroxide treated LPS (p>0.05). 4. The levels of all three cytokines released from PMN stimulated with P.endodontalis LPS were significantly lower than those released from PMN stimulated with E. coli LPS (p<0.05).

의미 분석 단계에서의 정보 흐름 제어

조현욱,김명수,정은환,유재휘,윤병주 명지대학교 공학기술연구소 1986 공학기술연구소 논문집 Vol.1 No.-

One of protection methods for computer internal data is to pursue data flow in program and to control its flow. In this thesis, we propose a mechanism which guarantees secure information flow in a program. By investigating security classes of all the variables in a statement and judging necessary condition for secure information flow, proposed model descriminates the program whether it is secure or not. Out model can be implemnted at language translation time, particularly semantic analysis phase, by pursuing security class transfer of each statement.

광주 신창동유적 : 제1차조사개보

조현종,장제근 한국고고미술연구소 1992 고고학지 Vol.4 No.-

이 글은 국립광주박물관이 광주시의 의뢰를 받아 실시한 광주 신창동 소재 유적에 대한 조사 약보(略報)이다. 유적은 광주-장성을 잇는 국도확포장구간에 포함되었고 공사의 진척에 따라 유적 일부가 잘려나가는 상황에 놓이게 되었다. 특히 유적 일대의 충적 저지대에서는 공사로 인해 패인 지표하 2m 정도의 깊이에 있는 황갈색 사질층과 흑갈색 진흙층에서 볍씨와 탄화미(炭化米) 등이 곡물자료와 함께 일부 가공흔이 나타나는 목재류 등이 채집되었다. 이에 광주박물관은 광주시 등 관계기관과 협의하여 1차로 공사구간에 대한 유구확인을 위한 발굴조사를 실시하게 되었다. 조사는 1992년 6월 26일부터 7월 25일까지 이루어졌다. 그 결과 국내에서는 처음으로 목제 농기구가 출토된 소택지(沼澤地)의 존재가 알려졌으며 이와 함께 초기철기시대에서 원삼국시대에 이르는 것으로 생각되는 토기요지·구상유구(溝狀遺構)·조상유구(條狀遺構)·주거지 등이 확인되었다. 소위 소택지에서는 흑도(黑陶)·고배(高杯)·점토대토기(粘土帶土器) 등 각종 토기류와 함께 칠기고배, 빗(櫛), 검파(劍把), 통발 등 다양한 목제유물과 볍씨 등 탄화미를 비롯한 곡물자료가 발견되었다. 특히 칠기고배를 비롯한 칠기 등과 목제유물은 지금까지 창원 다호리를 비롯한 영남지방에서는 드물게나마 출토된 바 있으나 한반도 서남부지역에서는 초출(初出)의 자료로서 시사되는 바 크다. 또한, 주거지를 제외한 각 유구에서 출토된 토기류의 형태적 특징에서 요지 출토품과 유사성이 인정됨으로써 당시의 생산과 유통에 관한 새로운 인식의 계기를 마련해 주고 있다. 이러한 물질자료들은 유적의 극히 일부에서 출토된 것이지만 아직 밝혀지지 않은 이 시기의 생산양식과 농경문화의 구체적인 실체에 접근하는 데 필요한 하나의 수확이며 고환경의 복원 및 문화발전과 전파문제의 해석에도 많은 도움이 될 것이다. The Shinchang-dong site in Gwangju, Jeollanam-do Province, was excavated by the Gwangju National Museum in 1992, prior to the construction of the national road. The excavation revealed a peat bog, a pottery kiln, a ditch-shaped feature, a dry field-like feature and a dwelling house. Among the materials recovered from the peat bog were 1) lacquer wares and wooden artefacts including a hoe, mounted dishes, a comb, a dagger handle, basketry fish traps, bird's head-shaped scuplture, and various tools for woodworking, 2) potteries including plain-coarse pottery, black-burnished pottery, and pottery with clay stripe along the rim, the shapes of which are mounted dishes, long-necked jars, wide-mouthed bowls, dish-shaped covers and steamers, 3) organic remains including grains and seeds of rice, barley, wheats, cucumber, legumes and apricots, walnut shells, fish bones and snails, 4) accumulations of large quantities of rice husks. Many wooden pieces, planks and tools with some indications of woodworking as well as the existence of posts for hanging wooden implements indicate the use of swamp as the place for woodworking. The pottery kiln was dug in the direction of contour. Its size is 8m in length by 2m in width, and the slopeness of the floor is 5~8°. It is a open kiln without any additional structure such as roof, wall and combustion chamber. The potteries found from the kiln are similar to those from the bog site. The floor plan of dwelling site is a rectangle about 430cm by 320cm, in the depth of 20cm. It has a central hearth in an oval shape, 50cm in diameter. The peat bog, the kiln and other features date to about the late 2nd century B.C. or the 1st century B.C. while the dwelling site dates to the 3rd century AD. Recognizing the importance of the site, it is designated as the NATIONAL HISTORIC SITE NO. 375.

다발성 골수종에서 저용량 thalidomide, cyclophosphamide, dexamethasone (TCD) 요법의 효과

류충헌,정재현,고정해,장제혁,박영진,최규남,박봉수,이상민,주영돈 인제대학교 2008 仁濟醫學 Vol.29 No.-

Background and Objectives : The immunomodulatory drug thalidomide can inhibit angiogenesis and induce apoptosis in experimental models. It can also induce marked and durable response in newly diagnosed myeloma patients. Thalidomide has been used at doses ranging from 200 to 800 mg with significant toxicity. No data are available on the impact of low-dose thalidomide, cyclophosphamide and dexamethasone as initial therapy for myeloma patients. Design and Methods : To address this issue, newly diagnosed myeloma patients were treated with 50 mg/day thalidomide continuously and cyclophosphamide 150 mg/m², days 1-4 and dexamethasone 20 mg/m², days 1-5 and day 15-19, every month. Between October 2005 and October 2006, 14 patients (median age 54.5 years) were treated with low-dose thalidomide, cyclophosphamide and dexamethasone. Results : After a minimum of two cycles of treatment, 5 patients (55.5%) showed a partial remission. After four cycles of treatment, 10 patients (83.3%) showed a partial remission (n=6) and complete remission (n=4). After a median follow-up of 15.4 months, 1 year overall survival rate was 82.0%. Thalidomide was well tolerated without serious toxic effects. Conclusions : The combination of low-dose thalidomide, cyclophosphamide and dexamethasone demonstrates favorable response rate and 1 year overall survival rate in newly diagnosed myeloma. Severe toxicities were not seen with this combination.

Scrub Typhus : 확진된 76예의 임상 소견에 대한 전향적 연구

김동민,김현리,박치영,윤성호,송현제,심수경 대한감염학회 2006 감염과 화학요법 Vol.38 No.4

배경 : Orientia tsutsugamushi에 의한 인체 감염은 혈액과 임파선을 타고 전신에 퍼져 혈관 염을 일으켜, 전신 장기의 침범 소견을 보인다. 그러나 아직 까지 전신장기 침범에 대한 임상 소견 및 검사 결과의 전향적인 추적 관찰에 대한 연구는 거의 없는 실정이다. 재료 및 방법 : 조선대학교병원에 내원한 환자 중 확진법(gold standard)인 간접형광항체법(IFA)으로. 확인된 76명의 환자를 대상으로 내원시 임상 양상 및 검사 결과의 평가 및 치료 후 검사 결과의 변화에 대한 전향적인 연구를 수행하였다. 결과 : 대부분의 환자에서 발열, 갈증, 전신 쇠약감, 두통을 호소하였으며, 특이 할만한 소견으로 현재까지 보고되지 않았지만, 대부분의 환자가 갈증을 심하게 호소한다는 것이다. 3명의 젊은 건강한 환자를 제외한 대부분의 환자에서 이러한 갈증 증상을 호소하였으며, 이러한 갈증은 발열이 호전되어도 지속되었으며, 퇴원 이후에 까지 지속되는 양상을 보였다. 혈액검사상 scrub typhus 초기에 나타날 수 있는 신기능 장애 및 간 기능 장애는 대부분 가역적으로 적절한 항생제 투여 후 모두 정상으로 회복됨을 확인할 수 있었다. 혈액 검사상 CRP, LDH, AST가 대부분의 환자에서 상승하였고, 특히 LDH의 상승이100% 환자에서 관찰된 것은 특이할 만 한데 적절한 치료후 CRP는 신속히 호전을 보이나, LDH는 서서히 호전됨을 확인하였다. DIC 검사상 DIC는 입원환자의 95.5%에서 확인되었으나, 비교적 일부의 환자에서 출혈 및 경색이 초래되는 것으로 생각된다. 결론 : Scrub typhus 초기에 나타 날수 있는 신기능 장애, 간기능 장애 및 DIC는 대부분 가역적으로 적절한 항생제 투여 후 모두 정상으로 회복되며, 이러한 관찰이scrub typhus 환자의 진단 및 임상 경과의 이해에 도움이 되리라 사료된다. Background : Orientia tsutsugamushi spreads to the entire body through the blood and lymphatics, and it induces vasculitis that results in the patients manifesting symptoms of systemic organ involvement. Materials and Methods : We conducted a prospective study to evaluate the clinical manifestations and the change of the laboratory results after instituting treatment for scrub typhus. Results : Most patients presented with fever (100%), malaise (96.1%) and thirst (96.1%). It was remarkable that most patients presented with severe thirst, except for 3 healthy, young patients. This thirst was persistent even after the resolution of fever. The renal and hepatic dysfunction were reversible after the administration of appropriate antibiotics. For the blood testing, it was deteded that the CRP, and LDH were elevated in most patients (95.9% and 100% respectively). DIC could be diagnosed in 95.5% of the patients at the time of admission Conclusion : Most patients presented with fever, malaise and severe thirst, and the renal and hepatic dysfunction were reversible after the administration of appropriate antibiotics. DIC was observed in most of our patients, but hemorrhage and infarction were not present. CRP showed a rapid improvement, nonetheless, the LDH and DIC test results improved slowly.

Optimal Dynamic Spectrum Access in Cognitive Radio Networks under Rayleigh Fading

Hyeon Je Cho,Gang Uk Hwang 한국산업응용수학회 2011 한국산업응용수학회 학술대회 논문집 Vol.6 No.2

We consider a time slotted cognitive radio (CR) network under Rayleigh fading where multiple secondary users (SUs) contend for spectrum usage over available primary users channels. In the contention, all SUs use common access probabilities to decide whether they access one of available channels. We focus on the throughput performance of an arbitrary SU. To improve the throughput performance of SUs, it is desirable that the access probabilities are adapted to the information on channel status and fading condition. In the analysis, we derive the optimal access probabilities that maximize the throughput performance of an arbitrary SU. From our analysis, we provide an insight on how to obtain optimal access probabilities in the CR network.

Workshops / WS1-1 : Full - automatic nucleic acid extraction system , TakaRa Genextractor TA-100

(Je Hyeon Lee) 한국생화학분자생물학회 (구 한국생화학회) 1999 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

Analysis of Optimal Random Access Policies for Cognitive Radio Networks under Rayleigh fading

Hyeon Je Cho,Gang Uk Hwang 한국산업응용수학회 2011 한국산업응용수학회 학술대회 논문집 Vol.6 No.1

We consider a time slotted cognitive radio network under Rayleigh fading where multiple secondary users (SUs) contend for spectrum usage over available primary users’ channels. We analyze a channel access policy where each SU stochastically determines whether to access a wireless channel or not based on a givne access probability. We focus on the queueing performance of an arbitrary SU. To improve the queueing performance of SUs, the access probability in our channel access policy is adapted to the information on channels, e.g., the number of available channels and the probability of a deep fade for channels. It is then important to obtain the optimal access probabilities from the queueing performance perspective. In this paper we consider three scenarios. In the first scenario, all SUs have full information on wireless channel status and fading channel conditions. In the second scenario, all SUs have full information on wireless channel status but do not know their fading channel conditions, and in the last scenario all SUs do not have any information on wireless channel status and conditions. For each scenario we analyze the queueing performance of an arbitrary SU and show how to obtain the optimal access probabilities with the help of the effective bandwidth theory. From our analysis we provide an insight on how to design an optimal channel access policy in each scenario. We also show how the optimal channel access policies in three scenarios are related with each other. Numerical results are provided to validate our analysis. In addition, we investigate the performance behaviors of the optimal channel access policies.

Workshop 3 : Takara Korea Biomedical ; Activities of Takara Bio Group in Asia

( Je Hyeon Lee ) 한국생화학분자생물학회 (구 한국생화학회) 2002 생화학분자생물학회 춘계학술발표논문집 Vol.2002 No.-

Molecular Cloning and Expression of a Sodium - Driven Flagellar Motor Component Gene ( motX ) from Vibrio fluvialis

(Je Hyeon Park),(Jong Hee Lee),(Young Sook Kim),(Yong Ki Hong),(In Soo Kong) 한국미생물생명공학회 2001 Journal of microbiology and biotechnology Vol.11 No.6

N/A N/A