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        • 고추炭疽病에 對한 新 浸透性 殺菌劑 Systhane M의 效果

          鄭鳳九,沈在燮 충북대학교 농업과학기술연구소 1986 農業科學硏究 Vol.4 No.-

          In order to investigate effect of the new fungicide systhane M for controlling red pepper anthracnose, this experiment was carried out in a field and indoor test during the summer months, June to September in 1986. Foliar applications of systhane M with the other 4 chemicals effectively reduced anthracnose fruits infected of red pepper compared with control. No chemical damage of the fungicides used was shown throughout the experiment. Because less than 500ppm of the fungicide was not completely retarded germination of the two causal fungi, it was recommended that effective concentration of the fungicide was more than 500ppm. In addition, further experiment regarding dates and times of the chemical application should be conducted in the future in order to obtain basic materials for establishing a feasible control calender.

        • Studies on new antibiotics in Korea 4

          Shim. Je-Seop,You-Jin Oh,Jeong-Ku Yun,Seong-soon Han 한국식물병리학회 1980 한국식물보호학회지 Vol.19 No.3

          '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        • Studies on new antibiotics in Korea IV

          Shim Je-Seop,Oh You-Jin,Yun Jeong-Ku,Han Seong-Soon Korean Society of Applied Entomology 1980 한국식물보호학회지 Vol.19 No.3

          본시험은 한국산항생물질 또는 항생균의 개발이용을 목적으로 한 기초연구로서 주로 전년도의 계속연구와 아울러 토양시료로부터 새로히 항생세균을 분리하였다. 청주부근의 토양시료 500점으로부터 83점의 항생균을 분리하였으며 이들은 주로 gram음성균인 Escherichia coli에 유효한 것을 선별하였다. 이들에 대한 연구는 앞으로 계속할 것이며 수년간 계속 연구중인 우수항생균 JS7501은 그동안 수차에 걸쳐 재선별하였기 때문에 JS901로 개칭하여 본시험에 공시하였으며 항균작용법 위도 재확인하였으며 주로 추출을 목적으로 용매추출, 흡착, TLC, 이온교환 수지 등을 총동원하였으나 TLC에 의하여 활성이 확인된 4 spot를 분리할 수 있었다. JS7901의 배양배지로서는 Soytone Sugar배지가 가장 높은 항균력을 보였으며 Soytone보다는 Sugar가 활성물질 증가와 관계가 있었다. The antibiotic bacterium JS7901, one of the eighty three antibiotic microorganisms which have been is elated in the surburbs of CheongJu-city, showed the most effective antimicrobial activities against test organisms, both bacteria and fungi. Among the different culture media Soytone Sugar medium was the most effective for growth and activity of the JS7901 antibiotic bacterium a against both Escherichia coli and Staphyllococcus aureus by the cylinder plate method. The higher the sugar content, was, the greater the antibiotic amount of substances of JS7901 were produced in the soytone sugar media. The antibiotic bacterium, JS7901 appeared to have a broad activity spectrum showing inhibition in Vitro against gram positive and negative bacteria and plant disease fungi. In general, the active substances were not transferred into organic solvents. Only a small portion of the activity was transferred into ethyl ether and was also adsorbed to active carbon when the cultured broth was also adsorbed to active carbon when the cultured broth was at $pH\;2.0\~4.0$. On adjusting at pH 8.0, the activity disappeared. The crude active substances could be obtained by means of vacuum drying method and still shelved strong activity. The dried active rake was solved by solvents and crystallized into various shapes. The active substances were developed on the silica gel plate in the solvent system of n-butanol-acetic acid-water(3 : 1 : 1) and gave 5 pinkish colored spots when sprayed with $0.2\%$ ninhydrine in ethanol. The upper 5th spot, which was the result of using disc plate method with Escherichia coli was the strongest of these spots.

        • KCI등재

          Studies on new antibiotics in Korea 4

          Shim Je-Seop,Oh You-Jin,Yun Jeong-Ku,Han Seong-Soon 한국응용곤충학회 1980 한국응용곤충학회지 Vol.19 No.3

          본시험은 한국산항생물질 또는 항생균의 개발이용을 목적으로 한 기초연구로서 주로 전년도의 계속연구와 아울러 토양시료로부터 새로히 항생세균을 분리하였다. 청주부근의 토양시료 500점으로부터 83점의 항생균을 분리하였으며 이들은 주로 gram음성균인 Escherichia coli에 유효한 것을 선별하였다. 이들에 대한 연구는 앞으로 계속할 것이며 수년간 계속 연구중인 우수항생균 JS7501은 그동안 수차에 걸쳐 재선별하였기 때문에 JS901로 개칭하여 본시험에 공시하였으며 항균작용법 위도 재확인하였으며 주로 추출을 목적으로 용매추출, 흡착, TLC, 이온교환 수지 등을 총동원하였으나 TLC에 의하여 활성이 확인된 4 spot를 분리할 수 있었다. JS7901의 배양배지로서는 Soytone Sugar배지가 가장 높은 항균력을 보였으며 Soytone보다는 Sugar가 활성물질 증가와 관계가 있었다. The antibiotic bacterium JS7901, one of the eighty three antibiotic microorganisms which have been is elated in the surburbs of CheongJu-city, showed the most effective antimicrobial activities against test organisms, both bacteria and fungi. Among the different culture media Soytone Sugar medium was the most effective for growth and activity of the JS7901 antibiotic bacterium a against both Escherichia coli and Staphyllococcus aureus by the cylinder plate method. The higher the sugar content, was, the greater the antibiotic amount of substances of JS7901 were produced in the soytone sugar media. The antibiotic bacterium, JS7901 appeared to have a broad activity spectrum showing inhibition in Vitro against gram positive and negative bacteria and plant disease fungi. In general, the active substances were not transferred into organic solvents. Only a small portion of the activity was transferred into ethyl ether and was also adsorbed to active carbon when the cultured broth was also adsorbed to active carbon when the cultured broth was at . On adjusting at pH 8.0, the activity disappeared. The crude active substances could be obtained by means of vacuum drying method and still shelved strong activity. The dried active rake was solved by solvents and crystallized into various shapes. The active substances were developed on the silica gel plate in the solvent system of n-butanol-acetic acid-water(3 : 1 : 1) and gave 5 pinkish colored spots when sprayed with ninhydrine in ethanol. The upper 5th spot, which was the result of using disc plate method with Escherichia coli was the strongest of these spots.

        • 新抗生物質 生産菌株의 選拔 및 利用에 關한 硏究 I

          沈在燮 충북대학교 엽연초연구소 1977 煙草硏究 Vol.4 No.-

          As a result of hard work, it has been possible to obtain 17 strains of antibiotic-organisms. Most of them were belonged to bacteria. Eleven strains of them could inhibit notonly gram positive and negative bacteria but also plant disease fungi in vitro. Therefore they should be belonged to a group of broad activity antibiotics, particularly the JS 7501 strain was remarkably shown the bread activity spectrum inhibiting many plant disease bacteria and fungi, as shown in table 2. The standared curve of the croup antibiotic substanceon the Staphylococcus aureus in H2O could be made as Fig 1. By the test of resistance of the antibiotic strain to 14 known antibiotics, the active substance formed by JS 7501 strain should be estimated to the group of peptide antibiotics such as penicillins, cephaloridine and colistine. But the antimicrobial activity spectrum of the strain differed from the group of peptide antibiotics. The antibiotic produced from JS 7501 strain would be a new antibiotic.

        • 葉煙草 病蟲害防除에 關한 硏究 : 5. 煙草 赤星病菌의 病原性에 關한 硏究 Studies on the Pathogenecity of Tobacco Brown spot Fungus, Alternaria longipes (Ell. et Ev.) Mason

          沈在燮,魯斗鉉 충북대학교 엽연초연구소 1971 煙草硏究 Vol.1 No.-

          According to the artificial inoculation method established by Nobuo Fukuda (1967), authors. obtained 8 Pathogenic strains out of 36 isolates of tobacco brown spot fungus. Five strains from the above 8 Pathogenic strains showed strong pathogenicities and 3 strains medium pathogenicities. The strongest strain, TA-68-l2 isolated froth Miwon, Chungbuk, Korea was applied for the testing of resistance of tobacco varieties and for the laboratory examination of fungicides to this disease. "Cheongju-eup" one of the Korean native varieties was found but the strongest resistant variety to tobacco brown spot among the 24 sample varieties. For the test of comparing the effects of fungicides by the artificial leaf inoculation method twelve kinds of fungicides and Y.S.A as a sample variety were used, and the results were as follows; Polyoxine E.C. and W.P. Dithane M-22 W.P and Orthocide W.P showed excellent effects of supressing the lesion expansion. Fermate W.P and PTA-B E C showed good effects against the lesion expansion on leaves. These fungicides shall be considered very usefull for controling the disease, if those be used after carefull research and improvement under the practical field contidion.

        • 담배 붉은별무늬병에 對한 品種間 抵抗性에 關한 硏究

          沈在燮 충북대학교 엽연초연구소 1976 煙草硏究 Vol.3 No.1

          Thirty-eight varieties of tobacco were tested both in the laboratory and field for susceptibility to brown spot caused by Alternaria longipes (Ell. et Ev.) Mason. 1) Laboratory test was carried out by the artificial inoculation method established by Nobuo Fukuda in 1967. The pathogenic isolate, TA 6812 was applied to the 36 sample varieties, including 11 Korean native varieties. Only one variety, Gwangtcho were resistant. Five varieties, I.Y.P, Jinhyang, Jinya, Ky 58 and Useultcho were medium. The remaining varieties were highly susceptible. No varieties were immune to the isolate TA 6812 of A. longipes. 1) They were also observed for their susceptibility in the field under the condition of natural occurence. Six varieties, Gwangtcho, I.Y.P, Jinhyang, Jinya, Ky 58 and Useultcho were medium resistant. No varieties were highly resistant to tobacco brown spot. However, the results of field test were mostly coincided with the results of laboratory.

        • 葉煙草 病蟲害防除에 關한 硏究 : 1. 煙草 赤星病菌 胞子飛散과 發病과의 關係 The Relationship between the spore dispersal and Seasonal disease development on the Tobacco Brown spot

          魯斗鉉,沈在燮 충북대학교 엽연초연구소 1971 煙草硏究 Vol.1 No.-

          This study was carried out for the purpose of forecasting and prevention the tobacco brown spot disease. The numbers of spore disporsal flying around the fields A, B and C were counted every day from June to August in 1968. The disease development was investigated from the one of the three fields during the above similar period. The meterological data was observed by the Cheongju, Meterological station. The results obtained are as fallow The spore dispersion curvets of the three tobacco fields have shown similar tendency each other. The highest peak of spore dispersal which was appeared before the occurence of the disease has shown in the middle of June in the case of korea. Several other peaks were found later during the occurence period of the disease. The spore dispersion curves seemed to he related with differences in temperatures and the precipitations. The occurence of this disease started to appear in the fields later the early of July and was closely related with the mean temperatures at 25℃. The older tobacco leaves grew and the upper the leaves grew up, the more the disease development increased. The virulent period of this disease was reached the peak at the latest harvesting stage of the leaf tobacco around the last of August.

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