백서 편측 설신경 손상 후 미각 및 설유두의 변화에 대한 연구

김윤태(Yoon-Tae Kim),전승호(Seung-Ho Jeon),염학렬(Hak-Ryol Yeom),강진한(Jin-Han Kang),안강민(Kang-Min Ahn),김성민(Sung-Min Kim),장정원(Jeongwon Jahng),박경표(Kyung-Pyo Park),이종호(Jong-Ho Lee) 대한구강악안면외과학회 2005 대한구강악안면외과학회지 Vol.31 No.6

Purpose of study: Lingual nerve damage can be caused by surgery or trauma such as physical irriatation, radiation, chemotherapy, infection and viral infection. Once nerve damage occurred, patients sometimes complain taste change and loss of taste along with serious disturbance of tongue. The purpose of this study was to evaluate the effects of unilateral lingual nerve transection on taste as well as on the maintenance of taste buds. Materials & Methods: Male Sprague-Dawley rats weighing 220-250g received unilateral transection of lingual nerve, subjected to the preference test for various taste solutions (0.1M NaCl, 0.1M sucrose, 0.01M QHCl, or 0.01M HCl) with two bottle test paradigm at 2, 4, 6, or 8 weeks after the operation. Tongue was fixed with 8% paraformaldehyde. After fixation, they were observed with scanning electron microscope(JSM-840A, JEOL, JAPAN) and counted the number of the dorsal surface of the fungiform papilla for changes of fungiform papilla. And, Fungiform papilla were obtained from coronal sections of the anterior tongue(cryosection). After cryosection, immunostaining with Gαgust(I-20)(Santa Cruz Biotechnology, USA), PLCβ2(Q-15)(Santa Cruz Biotechnology, USA), and T1R1(Alpha Diagnostic International, USA) were done. Immunofluorescence of labeled taste bud cells was examined by confocal microscopy(F92-300, Olympus, JAPAN). Results: The preference score for salty and sweet tended to be higher in the operated rats with statistical significance, compared to the sham rats. Fungiform papilla counting were decreased after lingual nerve transaction. In 2 weeks, maximum differences occurred. Gustducin and T1R1 expressions of taste receptor in 2 and 4 weeks were decreased. PLCβ2 were not expressed in both experimental and control group. Conclusion: This study demonstrated that the taste recognition for sweet and salty taste changed by week 2 and 4 after unilateral lingual nerve transection. However, regeneration related taste was occurred in the presence of preserving mesoneurial tissue and the time was 6 weeks. Our results demonstrated that unilateral lingual nerve damage caused morphological and numerical change of fungiform papilla. It should be noted in our study that lingual nerve transection resulted in not only morphological and numerical change but also functional change of fungiform papillae.

Design, Synthesis and Anticonvulsive Activities of Potential Prodrugs Linked by Two-carbon Chain

Zhao, Long-Xuan,Moon, Yoon-Soo,Basnet, Ar-Jun,Park, Jae-Gyu,Kim, Eun-kyung,Kim, Dae-Ok,Jeong, Tae-Cheon,Jahng, Yurng-Dong,Choi, Jong-Won,Lee, Eung-Seok The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.10

For the development of new anticonvulsive agents, GABAmimetics such as nipecotic acid, isonipecotic acid, ${\gamma}-aminobutyric$ acid (GABA), ${\gamma}-vinyl$ GABA (vigabatrin) and valproic acid were covalently coupled through an ester bond by a two-carbon linker chain as potential prodrugs and evaluated for their anticonvulsive activities.

비골 피판을 이용한 하악 및 하악과두 재건의 장기간 임상적 평가

안강민(Kang-Min Ahn),정헌종(Hun-Jong Chung),염학렬(Hak-Ryol Ryom),김항진(Hang-Jin Kim),김윤태(Yoon-Tae Kim),황순정(Soon-Jung Hwang),명훈(Hoon-Myoung),김명진(Myung-Jin Kim),김성민(Soung-Min Kim),장정원(Jeongwon Jahng),이종호(Jong-Ho 대한구강악안면외과학회 2005 대한구강악안면외과학회지 Vol.31 No.5

Purpose of study: The temporomandibular joint (TMJ) occupies a key functional role in mastication and contributes to normal deglutition, speech as well as cosmesis. When a large amount of mandible including the condyle head is resected, it is very difficult to reconstruct it as a functional unit. In this retrospective study, we present the functional, radiographic and cosmetic results of reconstructed temporomandibular joint using free fibular flap. Patients and Methods: Total 12 patients (M:F = 6:6) who underwent condylar reconstruction with the fibular flap were interviewed and examined by radiographs and Bio-PAK. Mean follow up periods was 47.7±20.0 months and the average age was 38.7±15.3 years. Remodeling of condyle and function of TMJ were evaluated and facial contour was judged subjectively. Results: All flaps were viable and no immediate postoperative complication had happened. One patient showed decreased mouth opening, so interpositional gap arthroplasty was performed. The resorption rates of reconstructed fibular were minimal and the condyle heads were changed into domeshaped neocondyle after 2 years. All patients had normal diet and no speech difficulty was reported. Nine patients were satisfied with their facial contour but three patients complained about the depression of cheek. Conclusion: The reconstruction of TMJ with free fibular flap was reliable methods and very effective means of restoring mandibular function. The functional and morphologic results were excellent and showed little complications.

관절경하 반월상연골의 수직 포위 봉합술 - 수술 수기 -

김정만,장윤종,Kim Jung-Man,Jahng Yoon-Jong 대한관절경학회 2003 대한관절경학회지 Vol.7 No.2

반월상연골의 변연부 파열 치료를 위한 봉합술에서 파열면의 완전한 접합을 위해 수직 포위 봉합술을 개발하였다. 이 술기는 반월상연골을 뚫어 손상을 주지 않으며 배우기 쉽고 시행하기 쉽다. To avoid incomplete coaptation of the peripheral tear of the meniscus an arthroscopic vertical enclosing meniscus suture technique was developed. In this technique the meniscus was not penetrated with any instruments or suture materials. This technique seems to be simple and easy to learn and to practice.

A report of 29 unrecorded bacterial species in Korea, belonging to the Alphaproteobacteria

Liu, Qingmei,Kim, Seung-Bum,Cho, Jang-Cheon,Yoon, Jung-Hoon,Joh, Ki-seong,Cha, Chang-Jun,Chun, Jong-sik,Seong, Chi-Nam,Bae, Jin-Woo,Jahng, Kwang-Yeop,Jeon, Che-Ok,Im, Wan-Taek The National Institute of Biological Resources 2015 Journal of species research Vol.4 No.2

As a subset study to discover indigenous prokaryotic species in Korea, a total of 29 bacterial strains assigned to the classes Alphaproteobacteria were isolated from various environmental samples collected from plant root, ginseng soil, forest soil, marsh, mud flat, freshwater and seawater. From the high 16S rRNA gene sequence similarity (>99.1%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to each independent and predefined bacterial species. There is no official report that these 29 species included in Alphaproteobacteria is have been described in Korea; therefore 14 species of 9 genera in the order Rhizobiales, 7 species of 6 genera in the order Sphingomonadales and 4 species of 2 genera in the order Caulobacterales and 3 species in the order Rhodobacterales and 1 species in the order Rhodospirillales found in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section.

The Change of the Mechanoreceptor of Anterior Cruciate Ligament in Fixed Knee and Injured Anterior Cruciate Ligament in the Rabbit

Seong Rae Cho(조성래),Hyoung Min Kim(김형민),Kee Haeng Lee(이기행),Youn Soo Kim(김윤수),Joo Hyung Lee(이주형),Yoon Jong Jahng(장윤종) 대한정형외과학회 2004 대한정형외과학회지 Vol.39 No.5

목적: 손상 받은 전방십자인대와 슬관절을 고정했을 때의 전방십자인대에서의 기계적 수용기의 변화를 알아보고자 하였다. 대상 및 방법: 18마리의 토끼를 각각 6마리씩 대조군, 관절고정군, 전방십자인대손상군으로 나누고 실험첫날, 관절고정군은 K-강선으로 고정하였고, 십자인대손상군은 인대의 경골부착부위를 절단하였다. 실험 제 1주, 3주, 5주에 각 군에서 2마리씩 도살한 후 조직학적 관찰을 위해 대퇴골부착부를 포함한 전방십자인대를 절취하였다. 기계적 수용기에 대한 조직학적 관찰은 수정된 gold-chloride 염색법을 이용하였다. 결과: 대조군의 정상 전방십자인대의 기계적 수용기는 제 1, 2, 3형으로 각 형간 출현 빈도에 차이가 없었다. 관절고정군에서는 대조군에 비해 제 5주에 기계적 수용기의 빈도가 유의하게 줄었다. 십자인대손상군에서는 수술 후 제 1주에 기계적 수용기의 수가 대조군에 비해 감소하여 수술 후 제 3주에는 수술 후 1주와 차이를 보이지 않다가 수술 후 5주에는 기계적 수용기가 보이지 않았다. 기계적 수용기의 형태학적인면에서 실험군 모두에서 시간이 경과함에 따라 붕괴정도가 심해졌으며, 관절고정군에 비해 전방십자인대군에서 그 정도가 더 심하였다. 기계적 수용기의 대부분은 표층 및 활액조직의 직하부층에 위치하였으며, 전방십자인대를 감싸고 있는 활액막의 변성 및 염증과 함께 거의 동시에 붕괴되었다. 결론: 전방십자인대의 기계적 수용기는 관절내 조건에 따라 변화하였다. 따라서 기계적 수용기를 보존하기 위해서는 슬관절의 고정이 필요한 경우 가능한 최소한의 기간만 고정시키고, 전방십자인대의 손상이 있는 경우에는 적응이 된다면 가능한 한 조기에 해부학적 수복을 해야 한다. Purpose: To identify the change of mechanoreceptor of anterior cruciate ligament (ACL) in fixed knee and injured ACL. Materials and Methods: Eighteen rabbits were divided into three groups as control, fixed knee, and injured ACL groups. Both knee of all fixed knee group were fixed with K-wire on the first day. The ligaments of the injured ACL group were cut at tibial attatchment site on the first day. Two rabbits of each group were sacrificed for the evaluation of mechanoreceptor at the 1st, 3rd, and 5th week. Histologic studies were performed by modified gold-chloride stain for the mechanoreceptors Results: The types of mechanoreceptor in normal ACL were type Ⅰ, type Ⅱ, and type Ⅲ. There was no difference in the appearance rate of each type. In the fixed knee group, the frequency of appearance decreased significantly at 5th week compared with that of the control group. In the injured ACL group, the frequency of appearance decreased at 1st week and showed no difference at 3rd week in comparison with 1st week, and zero at 5th week. In morphology the degree of degradation of mechanoreceptor increased with time in both experimental group, and showed more severe in the injured ACL group than in the fixed knee group. Most of all mechanoreceptors were located in the subsynovial region, and degraded with synovial degeneration and synovial inflammation. Conclusion: Mechanoreceptors were changed with the change of intra-articular condition and ligament itself. Therefore the duration of immobilization shoud be shortened if possible and the torn ACL should be repaired anatomically, if indicated, as soon as possible to preserve the mechanoreceptors.