서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

프리캐스트 포스트텐션 콘크리트 넓은 보-기둥 내부 접합부의 비탄성 거동

황재환,최윤철,김영찬,김대중,임재형,권기혁,문정호,이리형 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

The purpose of this paper is to evaluate the response of Precast Prestressed Concrete Interior Wide Beam-Column Joint subjected to cyclic lateral loading. To this end, the experimental investigation was performed with six half-scale specimens of interior connection. The design parameters are the beam to column depth ratio, and the number of tendons within column core. One of the specimens, IJH-6, is modeled for the connection at the top floor of a structure. The test results showed that the specimens sufficiently resist up to limiting drift ratio of 0.035 in accordance with the provisional standard by ACI of acceptance criteria for concrete special moment frames.

胎盤組織 Cytidine Deaminase에 關한 硏究 (Ⅰ) : Purification & Characterization

황병두,곽상태,임규,이재흔,백문기 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2

Cytidine deaminase has been partially purified from human term placenta approximately 26-fold by combination of ammoninm sulfate saturation, sephacryl S-300, DEAE-cellulose and hydroxyapatite chromatography, and then its properties have been investigated. The enzyme shows a broad optimum pH from 5 to 9 and is heat-stable being, almost remained its activity by heat treatment at 70℃ for 8 minutes. The enzyme activity is inhibited to 23% and 77% by 2 mM of Cu^2+ and Co^2+, respectively, but, other cations do not affect the enzyme activity. The apparant Michaelis constant for cytidine and deoxycytidine are 74 uM and 69 uM, respectively. Kinetic analysis of cytidine deminase in presence of 0.25 mM Cu^2+ shows that the nature of the inhibition to cytidine deaminase is competitive, considering that Vmax is independent while km value is increased. The enzyme activity is completely inhibited by 0. 5 mM of p-hydroxymecuribenzoicacid(PHMB)but all of the activity is recovered in adding mercaptoethanol. The enzyme activity is decreased about 50% in prescence of 5 mM of polyamines. The enzyme activity is mainly located in the nuclei (80%) and cytosol(16%) fractions. he molecular weight of the enzyme is about 770, 000.

수술로 절제된 간의 국소 결절성 증식의 임상적 특징

황남철,최문석,이준혁,고광철,백승운,유병철,이종철,이광웅,조재원,박철근 대한간학회 2004 Clinical and Molecular Hepatology(대한간학회지) Vol.10 No.2

목적: 간의 국소 결정성 과형성은 일반적으로 특별한 치료가 필요 없는 양성 종양이다. 그러나 드물지만 합병증이 발생한 경우와 진단이 불확실한 경우에는 수술을 시행하게 된다. 이에 저자들은 간절제술을 시행한 국소 결절성 과형성의 임상적, 영상학적 특징들을 알아보고자 하였다. 대상과 방법: 1995년부터 2003년까지 수술을 시행하여 조직을 확인한 국소 결절성 과형성 환자 10명을 대상으로 연령, 성별, 실험실 검사 및 영상 소견과 조직 소견을 분석하였다. 결과: 연령의 중앙값은 37.5세, 남녀 비는 1.5:1이었다. 5예에서는 간세포선종이나 간세포암종을 감별할 수 없어서 수술을 시행하였으며, 수술 후 우연히 진단된 예는 4예이었다. 간세포암종의 위험 인자를 가지고 있었던 예는 4예(B형 간염 바이러스 보유자 3예, 간경변 2예)이었다. CT검사가 시행되었던 6예 중 5예는 동맥기에서 조영 증강을 보였고 문맥기 및 지연기로 감에 따라 주변과 동일(4예)한 또는 감소(2예)되는 양상을 보여 간세포암종과의 감별이 어려웠다. 크기는 3.2±2.2㎝이었으며 위치는 간우엽의 후하구역(S6)에 가장 많았다(30.0%). 4예에서 수술 전 침생검을 시행하였으나 고분화 간세포암종이나 간세포선종과의 감별이 어려웠다. 결론: 임상상, 영상검사 및 간침생검으로도 간세포암종이나 간세포선종과의 감별이 어려운 경우 수술을 시행하였으며 수술 전에는 발견하지 못하였지만 다른 질환으로 인한 수술시 우연히 발견된 경우도 있어 국소 결절성 과형성의 수술 전 진단이 어려운 경우가 적지 않음을 알 수 있었다. Background/Aims: Focal nodular hyperplasia (FNH) is a benign hepatic tumor with few serious complications and no malignant transformation. However, differential diagnosis between FNH and other liver tumors, especially hepatocellular carcinoma, is often difficult. Methods: Clinical features of surgically resected FNH were reviewed. From January, 1995 to February, 2003, 10 patients with surgically resected FNH were enrolled. Their age, sex, results of laboratory examination, imaging studies and pathologic findings were evaluated. Results: Median age was 37.5 years and sex ratio (male:female) was 1.5:1. In 5 cases, resection to exclude hepatic adenoma or HCC was performed. Four cases were diagnosed incidentally after surgery. Four patients had risk factors for HCC, such as hepatitis B virus infection, liver cirrhosis or both. The size of FNH was 3.2 2.2 cm. The most common site of the tumor was segment 6 (30.0%). Differential diagnosis with HCC was difficult in 5 of six cases in whom CT was performed. Although needle biopsies were performed preoperatively in 4 cases, it was difficult to distinguish FNH from hepatic adenoma or HCC. Conclusions: FNH was resected due to uncertainty of diagnosis, or incidentally during hepatectomy in patients with other liver disease. In the former, differential diagnosis with hepatic adenoma or HCC was a major problem despite extensive work-up including dynamic CT or biopsy. (Korean J Hepatol 2004;10:135-141)

I-SSR 標識子를 利用한 호도나무屬의 識別 및 類緣關係 分析

黃錫仁,曺炅眞,李文鎬,李在善,李炳實,李郁 한국임학회 2004 한국산림과학회지 Vol.93 No.7

DNA 표지자를 이용한 호도나무 품종의 유연관계 및 품종구분을 위하여 I-SSR 9개 표지를 이용하여 104개의 유의적인 표지가 얻어졌으며, 신품종 호도나무를 포함한 83개 국내외 품종(종)에 대해 DNA 지문에 의한 품종식별이 가능하였다. 수원 1호, 수원 2호, 왕호도는 모두 Conrord, McKinster 등 미국에서 도입된 대립성 품종과의 인공교배로 육성된 품종으로 유전적 유연관계가 상촌 4호 등 국내 선발종보다는 산성 4호×Concord의 교배종과 더 가까웠다. 국내 선발종 호도나무인 상촌 2호, 상촌 4호 및 산성 4호 이들 끼리 한 그룹을 이루고 있는데 이는 국내의 호도나무가 외국품종과 구분되는 유전적 동질성이 있음을 보여주었다. 영동은 개체선발로 육성되었으나 외국종과 교배에 의하여 육성된 교잡종들과 같은 무리에 속하였는데, 이는 영동 품종은 외국 품종의 교배에 의해 외래 유전자 일부가 유입된 것이 아닌가 추정된다. Concord×상촌 1호., Concord×상촌 2호, Concord×대부 1호가 한 무리를 이루었고 Lake×상촌 2호가 이들과 구분되는 것은 교배차대가 유전적으로 화분수보다는 모수의 영향을 더 많이 받고 있음을 보여준다. Using 104 fragments amplified from 9 I-SSR primers, a total of 83 cultivars in Juglans spp. including new cultivars used for the present study were identified. The new cultivars 'Suwon No.1', 'Suwon No.2', and 'Wanghodo' are hybrids between the domestic cultivars and the introduced cultivars such as 'Concord' and 'McKinster' bearing big nuts. Consequently, these cultivars were closer to the hybrid between 'Sansung 4×Concord' than to domestically selected cultivars such as 'Sangehon 4' from the viewpoint of genetic relationship. The domestic cultivars such as 'Sangchon 2', 'Sangchon 4', and 'Sansung 4' belonged to the same group and were genetically distinctive from the introduced cultivars. This suggests that there would be difference in genetic structure between the domestic and the introduced cultivars. The cultivar 'Yeongdong' was clustered into the group of hybrids between the domestic and the introduced cultivars although it was developed by the selection of a tree from a domestic population. This might be because the genes were introduced from the introduced cultivars into the domestic population via open-pollination. The 'Concordx×Sangchon 1', 'Concord×Sangchon 2', and 'Concord×Daebu 1' were Clustered into the same group whereas 'Lake×Sangchon 2' was clustered into the different group. This ndicates that the genetic characteristics of hybrids may be influence more strongly by the mother tree than by the father tree.

경북 영천지방에서 발생한 마늘의 비대장애 현장조사

황재문 안동대학교 농업과학기술연구소 2000 農業科學技術 硏究論文集 Vol.7 No.-

胎盤 組織의 Protein Methylase II

황병두,이재흔,백문기 충남대학교 의과대학 지역사회의학연구소 1982 충남의대잡지 Vol.9 No.2

Protein methylase Ⅱ has been purified from human term placenta approximately 8,760 folds with a 14.5% yield. The enzyme shows sharp pH optimum around pH 5.8. The enzyme is easily inactivated by heat treatment for 5 minutes at 60℃, and when stored at -20℃ even in the presence of 10% glycerol, approximately 70% of activity has been lost for 8 weeks The enzyme doesn't require any divalent cations and Cu^2+ is a potent inhibitor, the activity being completely inhibited at 2 mM and 86% of the activity is recovered by addition of 4 mM EDTA. Histone IIA and myelin. basic protein arc good substrate for this enzyme. Km for S-adenosyl-L-methicnine and Ki for S-adenosyl-L-homocysteine are 2.03 x 10 exp(-6) M and 5. 8 x 10 exp(-7) M respectively. The methylated membrane protein from human placenta were analyzed by dodecyl sulfate/polyacrylamide gel electrophoresis. Band 1, 2, 4, 15, 17 are identified as 5 major classes of methyl-acceptor protein for protein methylase Ⅱ. The role of placental membrane prctein methylation is discussed with regard to placental function.

고추 국내외 수집 유전자원의 특성

황희숙,황재문,이우승,김점순,김병수 慶北大學校農業科學技術硏究所 1998 慶北大農學誌 Vol.16 No.-

국내외로부터 수집한 고추 유전자원을 포장에 재배하여 종자를 증식하는 동시에 주요 특성을 조사하였다. 헝가리에서 도입하여 1992년도에 평가한 계통들은 Hungarian Wax 혹은 Bell형으로서 바이러스에 심하게 걸려 우리포장환경에 잘 적응하지 못하였다. 1994년도에 평가한 계통들 중 브라질에서 풋마름병 저항성으로 도입한 KC350과 KC351은 직립성이며 바이러스에 강한 편이어서 매우 유망한 유전자원으로 사료되었다. 중국에서 도입한 KC376는 바이러스에 강한 편이며 대과를 착생하여 유망하게 보였다. 태국에서 도입된 계통들은 안동대에서 특성을 평가기록하였다. Accessions of pepper germplasm were planted in the field and their characteristics were recorded. Lines introduced from Hungary were very susceptible to virus diseases and as a result poor in adaptability in domestic field condition. KC350 and KC351 which were introduced from Brazil were growing errectly and tolerant to both bacterial spot and virus diseases. KC376, an introduction from China, was bearing large fruits with tolerance to virus. Introductions from Thailand were grown and evaluated in Andong Universiy, and major characters of them were recorded.

初期 胎盤組織의 Adenosine Deaminase

황병두,변해공,임규,이재흔,백문기 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

Two forms of adenosine deaminase (ADase) having molecular weights of 1,500,000 & 800,000 in the soluble fraction of the early placenta and plasma membrane bound ADase of the placenta were demonstrated respectively. Their properties and activity changes with gestational age have been investigated. ADases exhibit a broad pH optimum from 6 to 9 and heat labile, being completely inactivated by heat treatment at 70℃ for 10 min. Soluble fraction ADases and plasma membrane bound ADase are inhibited about 100% and 80% by s mM Cu^2+. The apparent Michaelis constant of pADase-cytotrophoblast(pADase-C) for adenosine is 110μM, and for deoxyadenosine 21μM, that of pADase-syncytiotrophoblast(pADase-S) is 80μM and 35μM, and that of plasma membrane bound ADase is 31μM and 26μM, respectively. Kinetic analysis of pADase-C and pADase-S in the presence of 10μM Cu^2+ show that the nature of the inhibition to pADase-C and pADase-S are noncompetitive and uncompetitive, respectively. The pADases (pADase-C + pADase-S) activity decreased with gestational age, the activity at term being about one-tenth that of the early placenta, while plasma membrane bound ADase increased with gestational age by about 2-fold. From above results it is discussed that pADase-C is related to cytotrophoblast, pADase-S to syncytiotrophoblast and plasma membrane bound ADase to cellular differentiation of placenta, and suggested that soluble fraction ADases of human placenta is different from plasma membrane bound ADase in their biological roles.

胎盤組織의 Adenosine Deaminase

황병두,이재흔,백문기 충남대학교 의과대학 지역사회의학연구소 1981 충남의대잡지 Vol.8 No.2

Adenosine deaminase(ADase) has been partially purified approximately 800-fold from human placenta, and its properties have been investigated. The enzyme exhibits a broad pH optimum from pH 6 to 8 and is heat-labile, being completely inactivated by heat treatment at 70℃ for 10 minutes. In contrast to other tissue ADase, the enzyme is inhibited by 2mM of Cu^2+ and Fe^2+ at pH 7, and by 2 mM of Cu^2+, Fe^2+, Co^2+ and Mn^2+ at pH 8. The inhibitory effect of Cu^2+ on the enzyme is so strong that the enzyme is completely inactivated by 20 μM of Cu^2+. The enzyme activity is easily recovered by EDTA(4 mM) in case of adding Cu^2+ after preincubation of enzyme with substrate, while the recovery is about 50% by treating Cu^2+ prior to addition of substrate. Thus substrate binding to enzyme seems to prohibit the Cu^2+ from binding to the enzyme. The apparant Michaelis constant for adenosine is 77μM, and the kinetic analysis in the presence of 2 μM Cu^2+ shows that Km is independent while Vmax is decreased. The molecular weight of the enzyme is about 800,000 and the apparant activity of the other type of ADase is not detected. From the above results it is suggested that the enzyme is different from other tissue ADase in its nature of protein and possibly biologic role.