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홍연규,엄재열,김순철,Jong-NaeHyun,Jae-MinCho 한국식물병리학회 2002 Plant Pathology Journal Vol.18 No.2
Effects of temperature and dew period on sporulation of a mycoherbicide, Epicoccosorus nematosporus, on the lesion of its host, Eleocharis kuroguwai were determined. Conidia formation was first observed after 10 days on plants incubated for either 12 or 16 h in a dew chamber at 28oC; 16 h dew period resulted in more conidia formation. As the dew period was decreased to less than 8 h, fewer conidia formed. Conidial production was most abundant at 28oC and produced as much as 3.3´104 conidia per lesion, while 0.1´103 and 2.3´103 conidia per lesion were produced at 16oC and 36oC, respectively. Alternating temperature regimes, i.e., 30/ 15, 30/20, 28/20, and 28/15oC (day/night) were much better than constant temperature, i.e., 30/30, 28/28/, and 20/20oC for sporulation. In the second sporulation, there were as much as 3.1´104 conidia per lesion (ca. <50% of the first sporulation). Then, sporulation dropped sharply to 6.2´102 conidia per lesion in the third sporulation. Results of this study suggest that temperature combined with dew period is the primary limiting factor in the use of E. nematosporus as a mycoherbicide of E. kuroguwai
홍연규,엄재열,이봉춘,이동창,송석보,Jae-MinCho,Jong-NaeHyun 한국식물병리학회 2003 Plant Pathology Journal Vol.19 No.2
The study was conducted to determine the cultural conditions and the effect of inert fillers for melanization and sporulation abilities of sodium alginate pellets, and the weeding efficacy of the formula in the field. Melanin production of E. nematosporus was affected by striking frequency. Percentage of melanized beads was increased to 80.6% at higher rpm up to 180. The melanized pellets produced more conidia with abundant mucilage than unmelanized pellets. Shaker culture of Epicoccosorus nematosporus with sodium alginate yielded a total of 55 mg per 100 pellets. Percentage of melanized pellets was highest, with 81.0% and 83.3% of melanization, when wheat bran and rice polish were amended and produced the conidia with 65.4 and 68.4 mg per 100 pellets, respectively. When 1 L of conidial suspension of 6.0 ´ 105 conidia per ml was applied on 30-day-old plants in a plot, 74.5% of the plants were killed within 20 days, whereas, its melanized sodium alginate pellets killed 57.8% of the plants in the same period. The number of tuber formation of Eleocharis kuroguwai in the untreated control plots was 128.5 per plot, but those of the plots treated with conidial suspension and melanized pellets were 22.1 and 39.7, respectively, at the end of the season. Results of this study showed that melanization of mycelia-mixed sodium alginate are an important sporulation factor in E. namatosporus as a mycoherbicide.
Photo or Solar Ferrioxalate Disinfection Technology without External Hydrogen Peroxide Supply
Min Cho,Joon Seon Jeong,Jae Eun Kim,Je Yong Yoon 대한환경공학회 2007 Environmental Engineering Research Vol.12 No.5
The Fenton reaction, which refers to the reaction between ferrous ions and hydrogen peroxide to produce the OH radical, has not been widely applied to the disinfection of microorganisms despite being economic and environmentally friendly. Cho et al. have previously proposed the neutral photo ferrioxalate system as a solution to the problems posed by the Fenton reaction in acidic conditions, 1) but this system still requires an external hydrogen peroxide supply. In the present study, we developed a simple disinfection technology using the photo or solar ferrioxalate reaction without the need for an external hydrogen peroxide supply. E. coli was employed as the indicating microorganism. The study results demonstrated the effectiveness of the photo ferrioxalate system in inactivating E. coli without any external hydrogen peroxide supply, as long as dissolved oxygen is supplied. Furthermore, the solar ferrioxalate system achieved faster inactivation of E. coli than an artificial light source at similar irradiance.