Coordinated change of a ratio of methylated H3-lysine 4 or acetylated H3 to acetylated H4 and DNA methylation is associated with tissue-specific gene expression in cloned pig

Kang, Jae-Ku,Park, Kwang-Wook,Chung, Yeon-Gu,You, Jueng-Soo,Kim, Yong-Kee,Lee, Seung-Hyeon,Hong, Seung-Pyo,Choi, Ki-Myung,Heo, Ki-Nam,Seol, Jae-Goo,Lee, Jong-Ho,Jin, Dong-Il,Park, Chang-Sik,Seo, Jeong 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Various cell types in higher multicellular organisms are genetically homogenous, but are functionally and morphologically heterogeneous due to the differential expression of genes during development, which appears to be controlled by epigenetic mechanisms. However, the exact molecular mechanisms that govern the tissue-specific gene expression are poorly understood. Here, we show that dynamic changes in histone modifications and DNA methylation in the upstream coding region of a gene containing the transcription initiation site determine the tissue-specific gene expression pattem. The tissue-specific expression of the transgene correlated with DNA demethylation at specific CpG sites as well as significant changes in histone modifications from a low ratio of methylated H3-lysine 4 or acetylated H3-lysine 9, 14 to acetylated H4 to higher ratios. Based on the programmed status of transgene silenced in cloned mammalian ear- derived fibroblasts, the transgene could be reprogrammed by change of histone modification and DNA methylation by inhibiting both histone deacetylase and DNA methylation, resulting in high expression of the transgene. These findings indicate that dynamic change of histone modification and DNA methylation is potentially important in the establishment and maintenance of tissue-specific gene expression.

Suppression of the NF-<i>k</i>B signalling pathway by ergolide, sesquiterpene lactone, in HeLa cells

Chun, Jae Kwang,Seo, Dong-Wan,Ahn, Seong Hoon,Park, Jae Hyun,You, Jueng-Soo,Lee, Chang-Hee,Lee, Jae Cheol,Kim, Yong Kee,Han, Jeung-Whan Pharmaceutical Society of Great Britain 2007 Journal of pharmacy and pharmacology Vol.59 No.4

A Real-Time Virtual Re-Convergence Hardware Platform

Jae Gon Kim,Jong Hak Kim,Hun Ho Ham,Jueng Hun Kim,Chan Oh Park,Soon Suk Park,Jun-Dong Cho 대한전자공학회 2012 Journal of semiconductor technology and science Vol.12 No.2

In this paper, we propose a real-time virtual re-convergence hardware platform especially to reduce the visual fatigue caused by stereoscopy. Our unique idea to reduce visual fatigue is to utilize the virtual re-convergence based on the optimized disparity-map that contains more depth information in the negative disparity area than in the positive area. Our virtual re-convergence hardware platform, which consists of image rectification, disparity estimation, depth post-processing, and virtual view control, is realized in real time with 60 fps on a single Xilinx Virtex-5 FPGA chip.

A Real-Time Virtual Re-Convergence Hardware Platform

Kim, Jae-Gon,Kim, Jong-Hak,Ham, Hun-Ho,Kim, Jueng-Hun,Park, Chan-Oh,Park, Soon-Suk,Cho, Jun-Dong The Institute of Electronics and Information Engin 2012 Journal of semiconductor technology and science Vol.12 No.2

In this paper, we propose a real-time virtual re-convergence hardware platform especially to reduce the visual fatigue caused by stereoscopy. Our unique idea to reduce visual fatigue is to utilize the virtual re-convergence based on the optimized disparity-map that contains more depth information in the negative disparity area than in the positive area. Our virtual re-convergence hardware platform, which consists of image rectification, disparity estimation, depth post-processing, and virtual view control, is realized in real time with 60 fps on a single Xilinx Virtex-5 FPGA chip.

금주 동맹의 참여에 영향을 미치는 주정 중독환자의 변인에 관한 예비적 연구

석재호,박유문,윤정섭 大韓神經精神醫學會 1992 신경정신의학 Vol.31 No.3

The author investigated the background of alcoholic patients which seemed to influence the participation of alcoholic patients to Alcoholics Anonymous. The subject of this study were 59 patients with alcoholic patients who had been admitted to Kangdong Sacred Heart Hospital from July, 1988 to September, 1990. This study was performed by the review of inpatient records and interview through telephone. The results were as follows : 1. As we compared the group of maintaining sobriety with the alcoholic group which had drank alcohol continusously after discharge. 1) The alcoholic patients who had professional job were more difficult to be abstinent than the other group(p=0.046). 2) There was a tendency that the continuously drinking group had significantly domineering mother and weak father(p=0.037). 2. As we compared participating group with non-participating group. 1) Alcoholic patients with drinking history over 15 years revealed resistance to participate in alcoholic anonymous(p=0.034). 2) Well-educted alcoholic patients attended alcoholic anonymous more than patients with lower educational level(p=0.0389). 3) Alcoholic patients in higher socioecconomic status had higher tendency to participate alcoholic anonymous than those in lower socioecconomic status(p=0.031). 4) those who eagerly participated alcoholic anonymous seems to maintain sobreity more than those who did not showed interest in alcoholic anonymous(p=0.0045).

Senescence and impaired DNA damage responses in alpha-synucleinopathy models

Yoon Ye-Seul,You Jueng Soo,Kim Tae-Kyung,Ahn Woo Jung,Kim Myoung Jun,Son Keun Hong,Ricarte Diadem,Ortiz Darlene,Lee Seung-Jae,Lee He-Jin 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

α-Synuclein is a crucial element in the pathogenesis of Parkinson’s disease (PD) and related neurological diseases. Although numerous studies have presented potential mechanisms underlying its pathogenesis, the understanding of α-synuclein-mediated neurodegeneration remains far from complete. Here, we show that overexpression of α-synuclein leads to impaired DNA repair and cellular senescence. Transcriptome analysis showed that α-synuclein overexpression led to cellular senescence with activation of the p53 pathway and DNA damage responses (DDRs). Chromatin immunoprecipitation analyses using p53 and γH2AX, chromosomal markers of DNA damage, revealed that these proteins bind to promoters and regulate the expression of DDR and cellular senescence genes. Cellular marker analyses confirmed cellular senescence and the accumulation of DNA double-strand breaks. The non-homologous end joining (NHEJ) DNA repair pathway was activated in α-synuclein-overexpressing cells. However, the expression of MRE11, a key component of the DSB repair system, was reduced, suggesting that the repair pathway induction was incomplete. Neuropathological examination of α-synuclein transgenic mice showed increased levels of phospho-α-synuclein and DNA double-strand breaks, as well as markers of cellular senescence, at an early, presymptomatic stage. These results suggest that the accumulation of DNA double-strand breaks (DSBs) and cellular senescence are intermediaries of α-synuclein-induced pathogenesis in PD.

Reversine increases the plasticity of lineage-committed cells toward neuroectodermal lineage.

Lee, Eun Kyung,Bae, Gyu-Un,You, Jueng Soo,Lee, Jae Cheol,Jeon, Yae Jee,Park, Jong Woo,Park, Jae Hyun,Ahn, Seong Hoon,Kim, Yong Kee,Choi, Wahn Soo,Kang, Jong-Sun,Han, Gyoonhee,Han, Jeung-Whan American Society for Biochemistry and Molecular Bi 2009 The Journal of biological chemistry Vol.284 No.5

<P>Functional dedifferentiation of lineage-committed cells toward pluripotency may have a great potential in regenerative medicine. Reversine has been shown to induce dedifferentiation of multiple terminally differentiated mesodermal origin cells, which are capable of being directed to differentiate into other cell types within mesodermal lineages. However, the possibilities of these cells to give rise to other lineages have not been examined. Here we show that large scale gene expression profiling of reversine-treated C2C12 myoblasts identifies a subset of up-regulated genes involved in specification of neuroectodermal as well as mesodermal lineages. Reversine treatment leads to up-regulation of priming genes of neuroectodermal lineages, such as Ngn2, Nts, Irx3, Pax7, Hes1, and Hes6, through active histone modifications in the promoter regions of these genes. Additionally, reversine increases the expression of markers for other cell types of mesodermal lineages, Ogn and apoE, via inducing active histone modifications, while down-regulating the myogenic basic helix-loop-helix factor, MyoD, via repressive histone modifications. Consistent with up-regulation of these genes, reversine-treated C2C12 myoblasts redifferentiate into neural as well as mesodermal lineages, under appropriate stimuli. Taken together, these results indicate that reversine induces a multipotency of C2C12 myoblasts via inducing a specific combination of active histone modifications. Collectively, our findings provide a mechanistic rationale for the application of reversine to dedifferentiation of somatic cells.</P>

천식 소아에서 혈청 렙틴과 만니톨 기관지 과민성과의 관계

유정경 ( Jung Kyung Yoo ),신재영 ( Jae Young Shin ),유정섭 ( Jueng Sup You ),정수인 ( Soo In Jeong ),송준섭 ( Joon Sup Song ),양승 ( Seong Yang ),황일태 ( Il Tae Hwang ),이하백 ( Ha Baik Lee ),백혜성 ( Hey Sung Ba다 ) 대한천식알레르기학회 2014 Allergy Asthma & Respiratory Disease Vol.2 No.1

Purpose: Epidemiological data indicate that obesity is a risk factor in asthma, however effects related to obesity and adipokines on airway inflammation and bronchial hyper-responsiveness (BHR) have not yet been demonstrated in the human airway. The aim of this study was to investigate the relationship between serum adipokine levels and BHR to mannitol in asthmatic children. Methods: Serum adipokine levels were measured and pulmonary function tests were perfomed: baseline, postbronchodilator inhalation, methacholine inhalation, and mannitol inhalation. The response to mannitol was expressed as the dose causing a 15% decrease in forced expiratory volume in one second (FEV1) (PD15), and as the response-dose ratio (RDR) (% fall in FEV1/cumulative dose). Results: Sixty-nine prepubertal children between the ages of 6 and 10 years were participated in the study. They comprised asthmatic children (n=40) and healthy (n=29). Twenty-two subjects (55.5%) with asthma had a positive mannitol bronchial provocation test (BPT) result. The body mass index (BMI) was higher in those asthmatics with positive mannitol BPTs than in asthmatics with negative mannitol BPTs and in the control group (19.30 kg/m2 vs. 17.60 kg/m2 vs. 17.93 kg/m2, P=0.035, P=0.046). Serum leptin levels were also significantly higher in asthmatics with positive mannitol BPTs than in asthmatics with negative mannitol BPTs and in the control group (10.58 ng/mL vs. 5.49 ng/mL vs. 6.75 ng/mL, P=0.002, P=0.016). Leptin values were significantly associated with a PD15 (r=.0.498, P=0.022) and RDR to mannitol (r=0.346, P=0.033) in asthmatic children after adjustment for BMI. Conclusion: Serum leptin levels were significantly associated with BHR to mannitol in asthmatic children. (Allergy Asthma Respir Dis 2014;2:30-37)

고려홍삼의 수지상세포 활성화 효과

김도순(Do-Soon Kim),박정은(Jueng-Eun Park),서권일(Kwon-Il Seo),고성룡(Sung-Ryong Ko),이종원(Jong-Won Lee),도재호(Jae-Ho Do),이성태(Sung-Tae Yee) 고려인삼학회 2006 Journal of Ginseng Research Vol.30 No.3

본 연구에서는 정관장 홍삼의 물(water) extract, 식용발효 주정 extract 및 홍삼 추출물로부터 분리 제조한 crude saponin을 이용하여 면역반응을 매개하는 수지상세포의 활성효과에 대하여 알아보았다. 그 결과 홍삼시료 중, crude saponin 100 ㎍/㎖을 처리하였을 때 수지상세포의 세포표면분자인 MHC class II, CD40, CD80, CD86의 발현이 증가하였으며, phagocytosis는 감소하였다. 또한 홍삼시료를 처리한 수지상세포와 allogeneic T세포를 함께 배양하였을 때, 홍삼시료의 물 extract, 식용발효주정 extract, crude saponin 모두 allogeneic T세포의 증식반응을 유도하였고, IL-2와 IFN-γ의 생산량을 증가시키는 것을 확인하였다. 또한 CD4? syngeneic T세포와 CD8? syngeneic T세포의 반응에서도 T세포의 증식반응을 높게 유도하였으며, CD4? syngeneic T세포에서 IL-2와 IFN-γ의 생산량을 증가시키고, CD8? syngeneic T세포에서는 IFN-γ 생산량을 증가시키는 것을 확인하였다. 이상의 결과로 crude saponin의 경우 수지상세포의 세포표면 공동자극분자의 발현을 유도하고 성숙을 유도함으로써 T세포의 활성을 증진시키는 것으로 생각되며, 물 extract와 식용발효주정 extract는 crude saponin과는 다른 기작으로 T세포 활성화를 유도하는 것을 알 수 있었다. 따라서 실험에 사용한 홍삼시료, 즉 물 extract, 식용발효주정 extract, crude saponin 모두 수지상세포의 활성을 유도하는 물질로써 암항원 특이적 T세포 활성화를 이용한 항암치료에 이용할 수 있는 가능성이 있다고 사료된다. Ginseng is a medicinal herb widely used in Asian countries. Dendritic cells(DCs) play a pivotal role in the initiation of T cell-mediated immune responses, making them an attractive cellular adjuvant for use in cancer vaccines. In this study, we examined the effects of Red-ginseng(water extract, edible and fermented ethyl alcohol extract, crude saponin) on the DCs phenotypic and functional maturation. Immature DCs were cultured in the presence of GM-CSF and IL-4, and the generated immature DCs were stimulated by water extract, edible and fermented ethyl alcohol extract, crude saponin and LPS, respectively, for 24hours. The expression of surface co-stimulatory molecules, including MHC(major histocompatibility complex) class II, CD40, CD80 and CD86, was increased on DCs that were stimulated with crude saponin, but antigen-uptake capacity was decreased. The antigen-presenting capacity of Red-ginseng extracts-treated DCs as analyzed by allogeneic T cells proliferation and IL-2, IFN-γ production was increased. Furthermore, CD4? and CD8? syngeneic T cell(OVA-specific) proliferation and IFN-γ production was significantly increased. However, CD4? syngeneic T cell secreted higher levels of IL-2 in responding but not CD8? syngeneic T cell. These results indicate the immunomodulatory properties of Red-ginseng extracts, which might be therapeutically useful in the control of cancers and immunodeficient diseases through the up-regulation of DCs maturation.

The Amniotic Fluid Proteome Differs Significantly between Donor and Recipient Fetuses in Pregnancies Complicated by Twin-to-Twin Transfusion Syndrome

김선민,Byoung-Kyu Cho,Byoung Jae Kim,Ha Yun Lee,Errol R. Norwitz,Min Jueng Kang,Seung Mi Lee,박찬욱,Jong Kwan Jun,Eugene C. Yi,박중신 대한의학회 2020 Journal of Korean medical science Vol.35 No.10

Background: Twin-to-twin transfusion syndrome (TTTS) is a serious complication of monochorionic twin pregnancies. It results from disproportionate blood supply to each fetus caused by abnormal vascular anastomosis within the placenta. Amniotic fluid (AF) is an indicator reflecting the various conditions of the fetus, and an imbalance in AF volume is essential for the antenatal diagnosis of TTTS by ultrasound. In this study, two different mass spectrometry quantitative approaches were performed to identify differentially expressed proteins (DEPs) within matched pairs of AF samples. Methods: We characterized the AF proteome in pooled AF samples collected from donor and recipient twin pairs (n = 5 each) with TTTS by a global proteomics profiling approach and then preformed the statistical analysis to determine the DEPs between the two groups. Next, we carried out a targeted proteomic approach (multiple reaction monitoring) with DEPs to achieve high-confident TTTS-associated AF proteins. Results: A total of 103 AF proteins that were significantly altered in their abundances between donor and recipient fetuses. The majority of upregulated proteins identified in the recipient twins (including carbonic anhydrase 1, fibrinogen alpha chain, aminopeptidase N, alpha-fetoprotein, fibrinogen gamma chain, and basement membrane-specific heparan sulfate proteoglycan core protein) have been associated with cardiac or dermatologic disease, which is often seen in recipient twins as a result of volume overload. In contrast, proteins significantly upregulated in AF collected from donor twins (including IgGFc-binding protein, apolipoprotein C-I, complement C1q subcomponent subunit B, apolipoprotein C-III, apolipoprotein A-II, decorin, alpha-2-macroglobulin, apolipoprotein A-I, and fibronectin) were those previously shown to be associated with inflammation, ischemic cardiovascular complications or renal disease. Conclusion: In this study, we identified proteomic biomarkers in AF collected from donor and recipient twins in pregnancies complicated by TTTS that appear to reflect underlying functional and pathophysiological challenges faced by each of the fetuses.