N-alkyl/perfluoroalkyl 관능기의 표면특성

김재원,하종욱,박인준,소원욱,이수복 한국공업화학회 2004 응용화학 Vol.8 No.1

Micro slide glass surfaces was coated by the self-assembled technique with a monolayer of hydrocarbon/fluorocarbon(HC/FC) compounds, such as CH₃(CH₂)_(n)C₂H₄Si(CH₃)₂Cl (n=3, 5, 7, 9) and CF₃(CF₂)_(n)C₂H₄Si(CH₃)₂Cl(n=5, 5, 7, 9). These meterials was synthesised by the hydrosilation reaction. These monomers was characterized using nuclear magnetic resonance(NMR). The effect of concentration of HC/FC on coating quantity was investigated. Sessile drop measurment was used for quantitative chracterization of formation for the monolayer. An increase of concentration of HC/FC raised contact angle of water and methylene iodide. Contact angle of water and methylene iodide was increased according to an increment of alkyl chain length.

Minicell 분리에 관한 연구

고재문,김종세,김성준,노영복 조선대학교 기초과학연구소 1989 自然科學硏究 Vol.12 No.1

Minicell은 간상 박테리아의 극단에서 변이 세포분열에 의해 생산되는 작은 비성장체이다. 그것은 protein이나 RNA를 함유하나 chromosomal DNA가 조금 있거나 거의 없고 모양은 구형이다. 본 실험을 통해 얻은 결론은 다음과 같다. A. Minicell 생산이 왕성한 x1411 균주를 12시간 배양한 후 optical density는 ML media=0.25, M9 minimal media=0.5, x1776 broth media=0.7, 3×D media=0.9, LB media=1.0으로 나타났다. B. x1411 균주와 pOX7의 형질전환율은 2×10^5개/㎍·DNA로 나타났다. C. 변형된 방법에 의해 분리되어진 minicell양은 1.8×10^4cells/㎖로 나타났다. D. Minicell의 중심부와 극단에서 생성된다는 보고와는 달리 본 실험을 통한 관찰 결과 minicell은 중심부에서는 생성되지 않고 극단에서만 생성된다. E. 전자현미경을 통한 관찰 결과 host cell과 minicell의 크기비는 10:1 정도며, 모양은 host cell은 간상이고 minicell은 구형이다. Minicells are small non-growing bodies produced by aberrant cell divisions at the polar ends of rod-shaped bacteria. They are anucleate, contain RNA and protein but little or no chromosomal DNA fragment and approximately spherical in shape. The results are summarized as follows ; A. After culturing 12 hours, cell density of typical minicell-producing χ 1411 strains appeared to 0.25 in ML media, 0.5 in M9 minimal media, 0.7 in χ 1776 broth media, 0.9 in 3XD media and 1.0 in LB meda. B. The transformation rates between χ 1411 strains and pOX7 plasmid were 2×10^5 in each ㎍. DNA. C. Isolated minicells with modified method were 1.8×10^4 in each ml. D. some papers reported that minicells are produced at the polar ends and the central part, but in this experiment minicells were only produced at the polar ends. E. The results of observation with E.M. ; the ration of cell size between the host cell and the minicell was about 10 : 1, host cell was rod type in shape and minicell spherical type.

Baculovirus를 이용한 Aujeszky's Disease Virus gⅢ 단백질 발현

송재영,이중복,현방훈,박종현,김병한,권창희,전무형,안수환 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

The g Ⅲ gene located in U_L region of Yangsan strain, a field isolate of Aujeszky's disease virus (ADV) in Korea, was cloned into pTZ18R and sequenced. The gⅢ gene consisting of 1,437 nucleotides showed 98% sequence homology with that of Becker strain, a reference strain of ADV. The gene encoding gⅢ of Yangsan strain was placed under the control of Autographa californica nucleopolyhedrosis virus (AcNPV) polyhedrin promoter, and expressed by the derived recombinant baculovirus using Spodoptera frugiperda 9 (Sf9) cells. The expressed gⅢ was a protein with molecular weight of 72kd determined by immunoprecipitation and Western blotting assay using anti-ADV polyclonal antibodies and anti-gⅢ monoclonal antibody. The partially purified gⅢ protein was utilized as antigen in the radial immunodiffusion enzyme assay (RIDEA) to detect to specific antibody against ADV in pig sera. The results indicated that the sensitivity of RIDEA with the recombinant gⅢ protein antigen (98%) was as high as that with the conventional glycoprotein antigen extracted from the ADV infected cells. In addition, the false positive and false nagative reactions in gⅢ RIDEA were significantly reduced than the conventional glycoprotein RIDEA as judged from the results of standard serum neutralization test.

大邱地方의 頭部白癬

徐舜鳳,全在福,金基洪,金仁珠 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

1976年부터 1979年까지 慶北 漆谷카톨릭皮膚科病院에 來院한 外來患者中에서 臨床的 및 眞菌學的으로 頭部白癬으로 確診된 154例에 對하여 觀察한 成積을 다음과 같이 要約한다. 頭部白癬은 總外來患者의 0.19%에 該當하며 1976年부터 1978年까지는 큰 變動이 없었으나 1979年에 갑자기 2~3倍로 增加되었다. 男女의 罹患比는 7歲未滿에서는 큰 差異가 없으나 8~14歲群에서는 男子가, 高年齡群에서는 女子가 높았다. 그러므로 1961年以前에 比하여 男女間에 큰 差異가 없어지고 있다. 總分離菌 136株中 M. canis가 97株(71.3%), T. ferrugineum이 26株(19.1%), T. mentagrophytes가 10株(7.4%), T. rubrum이 3株(2.2%)이었다. 이 成績이 1961年前에 比하여 T. ferrugineum은 1977年부터 顯著히 減少되는 反面에 M. canis가 急增되어 主要菌의 交替現象이 일어나고 있다. T. ferrugineum은 14歲未滿의 男子에 많고 M. canis는 男女 모두 14歲未滿에서 많았으나 特히 女子에서는 7歲未滿과 高年齡群에 많았다. 特히 M. canis感梁이 7歲未滿 兒童에 많았으므로 앞으로 感染源인 고양이의 索出과 이에 對한 適切한 加療가 必要하다. Reports have been made of the detailed clinical and mycological observation on tinea capitis in the Taegu area during the period of 1954 through 1961. There had been, however, no follow-up studies on the disease until 1975. With the reports on the increasing incidence of Microsporum canis infections throughout the country, we suppose that the clinical features and the causative agents of tinea capitis may differ from those observed between 1954 and 1961. The authors observed 154 cases of tinea capitis at Chilgok Skin Clinic during the period from 1976 to 1979, clinically and mycologically. Tinea capitis occupied 0.19% of total outpatients in the clinic, and the incidence increased markedly during 1979. There was no general difference in incidence rates between both sexes of children, but a tendency was noted male children were predominant among the age group of 8 to 14, while female children were predominant among the age groups of under 7 and over 40 years. Among 136 fungi isolated from patients M. canis was most frequently found with 97 (71.3%) strains, and followed by 26(19.1%) of Trichophyton ferrugineum, 10(7.4%) of T. metagrophytes and 3(2.2%) of T. rubrum. These results indicate that T. ferrugineum which was the most predominant agent of tinea capitis before 1962, is decreasing markedly, while M. canis is increasing rapidly as the causative agent. The number of strains isolated in 1979 was more than the total number of strains in 1976 through 1978, showing that the predominance of causative agents in tinea capitis is changing toward M. canis, as compared with the distribution of agents before 1962. T. ferrugineum was frequently isolated from male children under the age of fourteen. M. canis was predominant in both sexes under the age of fourteen, but was predominant among patients under the age of seven and a higher age group in female.

지루피부염에 대한 ketoconazole 샴푸의 효과

이석종,전재복,김도원,정상립 慶北大學校 醫科大學 1994 慶北醫大誌 Vol.35 No.4

목적 : Pityrosporum ovale는 지루피부염의 발생에 중요한 역할을 하며 이 친지성 진균은ketoconazole제재에 감수성이 있다고 알려져 있다. 이에 저자들은 두피에 사용하기 편리한 2% ketoconazole 샴푸로 지루피부염을 치료하여 그 효과를 확인하고자 본 조사를 실시하였다. 대상 및 방법 : 두피의 지루피부염 환자 52명에게 소양감의 정도를 설문조사하고 피부과 의사의 육안으로 인설의 양을 검사한 후 광학현미경 고배율하에서 진균학적 검사를 실시하였다. 결과 : 지루피부염 환자의 소양감과 인설의 양은 치료전에 비해 치료 2주와 4주 모두에서 유의하게 감소하였으며(p<0.05) 이들 환자에서 실시한 Pityrosporum ovale의 평균 포자수 역시 치료전에 비해 치료 2주와 4주 모두에서 유의하게 감소하였다(p<0.05). 결론 : ketoconazole 샴푸는 지루피부염의 임상 및 진균학적인 면 모두에서 치료효과가 있는 것으로 판단되며 간접적으로나마 Pityrosporum ovale가 지루피부염의 발생에 관여한다는 사실을 알 수 있었다. It is suggested that Pityrosporum ovale may play an important role in the pathogenesis of seborrheic dermatitis and this organism is sensitive to ketoconazole compound. So the efficacy of 2% ketoconazole shampoo in the treatment of seborrheic dermatitis was tested and was calculated by mean scores of scale, itching and yeast cells. We interviewed fifty two patients with seborrheic dermatitis about the degree of itching and examined with naked eyes the scale of scalp of them. In a group of the patients, mycological study of P. ovale was also performed by direct smear with 20% KOH solution containng 1 : 1 Parker-ink^R The results were as follows : As for itching and scale of the scalp, the scores were significantly decreased at week 2 and week 4 as the duration of treatment prolonged in comparison with that of week 0. The mean yeast cell score at the two points of time was also significantly decreased in comparison with that of week 0. There was no significant difference in the response from the view of age, sex and duration of seborrheic dermatitis. It is suggested that the shampooing with ketoconazole 2% shampoo every three other days gave a good result clinically and mycologically.

발견학습을 통한 수학수업의 능률 향상에 관한 연구 : 중1학년 입체도형의 "측정"단원을 중심으로 Focusing on a chapter of "measurement" of a solid figure targeting students in the 1st grade of junior high school

김지영,전재복 국민대학교 2003 기초과학연구소 논문집 Vol.22 No.-

Criticism grows on current mathematics lessons at school lack of mathematical ability and just inclined to the acquirement of fragmentary knowledge and proficiency in solving questions. As a method of enhancing the mathematical ability, discovery learning has been selected and introduced to the class to make the class more interesting and fruitful. Therefore, the objective of this study is to obtain positive effects on school records by raising the interests and concerns of students about mathematics. Through such a research, the study concludes as followings: First, the studying-learning that lays stress on voluntary operations and discovery takes more positive effects on school records than the cramming studying/learning centered on explanation, exercise and memorizing. As a result, the mathematical lesson conducted through the discovery learning is more effective on the improvement of school records by arousing interests of the students. Second, through analyzing answers for the question papers asking the mathematical tendencies, attitudes and interests to both the experimental group and the control group and carried out after applying the discovery learning, it has been proved that the experimental group rather than the control group takes more positive effects on the affective domain. According to the above research, it is revealed that the mathematics lesson adopting the discovery learning shows more positive effects on school records by drawing interests of students in mathematics. Hence it is a task to be solved in the future to develop effective teaching and learning media with regard to mathematics and to make a continuous study on the establishment of mathematics as interesting and effective learning.

국내에서 분리된 canine parvovirus의 구조유전자 cloning과 염기서열 분석

박종현,송재영,이중복,현방훈,안수환,전무형 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

In this study gene encoding structural proteins of a CPV isolate was cloned and saquenced to elucidate the molecular genetical properties of the canine parvoviruses isolated from the field. Six recombinant plasmids of pEP3, p1471, p2070, pEP069, pEP338 and p14711p were constructed from the map positions 22 to 98 of RF DNA to clone the VP1 and VP2 genes of CPV-V20. Sequentialy the gene comprising 3780 nucleotides were sequenced by dideoxy chain termination method. When nucleotide sequence of gene encoding the structural proteins of CPV-V20 was compared with those of other strains, CPV-N, CPV-d and CPV-780929 published previously. DNA homologies to CPV-V20 were 99.87% with CPV-NM, 99.73% with CPV-d, 96.85% with CPV-780929 AND 98.4% with FPLV-Carl, respectively. The DNA sequence data of CPV-V20 showed seven point mutations and also deletion of 135 nucleotides from the nucleotide position 4745 to 4879 located in the 3-noncoding region of CPV-N.

체육 교육과정 영역별 구성에 관한 연구

정종훈,김재호,정광복,차봉준 東亞大學校附設스포츠科學硏究所 1998 스포츠科學硏究論文集 Vol.16 No.-

The purpose of this study is to create the living Sports for All boom which can participate everyone by using mass media for activation of the living Sports for All in Pusan, and owing to the city's negative policy for various problematics of the living Sports for All; such as the lack of public relation of the concerned authorities, a lack of an association of like-minded persons' support, shortage of P.R. Professional manpowers, owing to the negative policy against the city or district office's administrative, organizational, budgets etc, therefore, the P.R. of living Sports for All and the base enlargement were fall behinded than other cities, so that, the study aimed to establish various P.R. methods for the sake of citizen's health and to rise participation rate of the living Sports for All activity. Utilizing method of effective public relation of mass media for Pusan city's living Sports for All activations are as follows: If we publisize by considering the establishment of correct P.R. landmark through mass media and establishment of P.R. budget, choice of various mass media, drawing up P.R. draft, conclusion of P.R. program and the survey if P.R. effect then we will expect the utmost effect. Like this the conclusion of P.R. activation methods of the living Sports for All through mass media are as follows: First ; In other to spread the effect of living Sports for All P.R. we must consider the diversity of public relations, establishment of accurate goal of P.R.media, consideration of P.R. media's drawing up, consideration of daily program of P.R. media and consideration of continuous survey and analysis of the P.R. effect by using various mass media (such as : printing, broadcasting and the outdoors) Second : We must consider the support against the like minded person's activity, harmony of the elite physical education and the school Sports for All and the extension of the security and investment of the job site and the region and school Sports for All facilities. Third : In order to plan the activation of the living Sports for All through mass media, we must consider, there need establishment of exclusive public relation's post, establishment of system's organization, cultivate professional manpower and increase of P.R. budget and sustain the relation with the press continuously.

Pocine Adenovirus-3의 E1B Region의 鹽基序列 分析

朴鍾賢,宋載永,李重馥,玄芳勳,安東濬,車相昊,裵用泰,姜永源,Reddy, P S,全茂炯,安壽煥 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

돼지 아데노바이러스(PAV-3). 6618주의 EIB region이 包含되어 있는 map unit 4.0에서 9.7까지의 유전자에 대한 1,984 bp의 염기서열을 決定하였으며, 이 結果를 알려진 여러 아데노바이러스 유전자와 비교하여 다음과 같은 결과를 얻었다. 1. PAV-3의 EIB유전자는 10개의 ORF로 구성되어 있으며, 그 중 아데노바이러스의 단백질과 유사성이 있는 것은 ORF1, ORF2 및 ORF3이었다. ORF1은 Ad41의 19kd 와 BAV-2에서의 EIB ORF2에서의 아미노산의 一致率은 각각 32%와 31%이었다. 2. ORF2는 Ad2 55kd protein과 tupaia adenovirus 44kd protein가 각각 34%로 아미노산 一致率이 가장 높았으며, Ad41의 52kd protein. BAV-3의 EIB ORF3에서도 33%의 一致率을 보였다. 3. ORF1은 61-666 uncleotide (606 bp), ORF 2에서는 429-1,850 uncleotide (1,422 bp)의 부위로 각각 202, 474 a.a로 構成되었으며, 예상되는 분자량은 20 kd와 52 kd이었다. 4. ORF3는 hexon-associated pIX유전자로 추정되며 내부에 1개의 polyadenylation signal(ATAAA)이 1938-1942 uncleotide에 위치하였으며, 이 부위는 TATA box (1937-1942 uncleotide)와 중복되어 존재하였다. Porcine adenovirus type 3 (PAV-3) does not cause severe infection in pigs. Adenovirus has been suggestive of live vaccine vector carrying foreign gene. One of insertion regions is delayed early (EIB) region. However, EIB region of PAV-3 has not been molecularly characterized to date. Nucleotide sequence of EIB of PAV-3 was determined. The EIB region was composed of 1,984 bp and located between 4.0 and 9.7 map units. Three potential open reading frames(ORFs) with low level of homology to other adenoviruses and a polyadenylation signal were identified in the rightward direction of genome. The nucleotide and the predicted amino acid sequences of EIB were compared to those of human and animal adenoviruses. One of the three potential ORFs. ORF1 encoded a polypeptide homologous to bovine adenovirus type 2(BAV-2) ORF2 and human adenovirus type 41(Ad41) 19 kd protein. ORF2 encoded a polypeptide homologous to human adenovirus type 2(Ad2) 55 kd protein, bovine adenovirus type 3(BAV-3) ORF3 and porcine adenovirus type 4(PAV-4) ORF2. The predicted protein of ORF1 had homology to those of Ad41 and BAV-2 with 32 and 31% respectively, whereas the deduced protein of ORF2 had homology to those of Ad2. BAV-3 and PAV-4 with 34, 33 and 29%, respectively.

노령에서 고관절 골절에 영향을 주는 수상전 요인

김종오,윤여헌,고영도,유재두,정준모,방한천,강규복 대한골절학회 2002 대한골절학회지 Vol.15 No.4

목 적 : 노령에서 고관절골절의 여러 원인적 요인들과 상관관계를 분석, 그 연관성에 대해 알아 보고자 하였다. 대상 및 방법 : 2001년 1월부터 2002년 5월까지 대퇴 고관절 골절로 본원에서 치료받은 환자중 60세 이상이고 골밀도 검사를 시행받은 158례를 대상으로, 수상전 요인을 나이, 성별, 흡연여부, 비만정도, 골절종류, 수상장소, 다른 이환 질환, 골절전 활동도, 골밀도 검사, 수상당시 바닥의 경도, 수상당시 넘어진 방향, 수상에너지에 따라 대퇴 경부골절과 전자간 골절, 전자간 골절중 안정골절과 불안정 골절로 나눠 비교하였다. 결 과 : 80세 이상의 군에서 대퇴 전자간 골절이 대퇴 경부 골절에 비해 의미있게 많이 발생했음을 알 수 잇었으며(p<0.001) 환자의 수상전 활동정도의 비교에서도 활동정도가 낮았던 군에서 대퇴 전자간 골절이 의미있게 많이 발생하였다(p<0.001). 골밀도 검사에 따른 비교에서는 T-score -3.0 이하인 군에서 대퇴 전자간 골절이 많았으며(p<0.005), 수상에너지에 따른 비교에서는 적은 에너지에 의한 골절이 전자간 골절에서 많았다(p<0.05). 대퇴 전자간 골절은 안정 골절과 비안정 골절로 나눈 비교에서는 사회 활동이 어려운 ADL C이하의 군과(p<0.05), 골밀도 T-score -3.0 이하인군에서는 의미있는 차이를 보였다(p<0.001). 결 론 : 노령에서의 고관절 골절 중 80세 이상의 고령에서, 골밀도 T-score -3.0 이하인 경우, 일상생활 활동정도가 낮은 경우에, 비교적 적은 에너지에 의한 충격시에 대퇴 전자간 골절의 발생이 대퇴 경부 골절에 비해 의미있게 많았고 전자간 골절에서 불안정 골절은 사회활동이 적고 골밀도 T-score -3.0 이하인 경우 많음을 알수 있었다. Purpose : We studied the co-relation on the causes of the hip fracture through the analysis of a relevance on the etiological factors as increased incidence according increasing old age. Materials and Methods : Total 158 cases that treated on the hip fracture from 2001 Jan. to 2002 May were studied. The parameters were age, gender, smoking, obesity, type of fracture, place of fracture, other comorbidity, activity of daily living, bone marrow densitometry, hardness of floor, orientation, injury energy. And then, we analysis of difference between femur neck fracture and femur intretrochanter fracture and between stable femur intertrochanter fracture and unstable femur intertrochanter fracture. Results : The incidence of the femoral intertrochanteric fracture was larger significantly than that of the femoral neck fracture in the older then 80(p<0.001). On the comparison of the pre-fractural activity of daily living, the group revealed lower activity had larger incidence of femoral intertrochateric fracture(p<0.001). Also, the femoral intertrochanteric fracture was more larger osteoporosis patient group(p<0.005), and lower energy trauma(p<0.05). In a unstable femoral intertrochanteric fracture, 21 cases(77.7%) of total 27 cases were belong to the group of the activity of daily living scale below C(p<0.05), 24 cases pf 27 cases were belong to the osteoporosis patient who was estimated below -3.0 on T-score(p<0.001). Conclusion : In the hip fracture of the elderly patients, the femoral intertrochanteric fracture is more prevalance ratherthe femoral neck fracture on the cases of older patient more than 80 year-old, lower activity of daily living scale, lower T-score less than -3.0 on BMD, lower energy trauma. Also, in femur intertrochanter fracture, unstable fracture is more common in low daily activity and owteoporosis.