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      • SCIESCOPUSKCI등재

        Selection for Duration of Fertility and Mule Duck White Plumage Colour in a Synthetic Strain of Ducks (Anas platyrhynchos)

        Liu, H.C.,Huang, J.F.,Lee, S.R.,Liu, H.L.,Hsieh, C.H.,Huang, C.W.,Huang, M.C.,Tai, C.,Poivey, J.P.,Rouvier, R.,Cheng, Y.S. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.5

        A synthetic strain of ducks (Anas platyrhynchos) was developed by introducing genes for long duration of fertility to be used as mother of mule ducklings and a seven-generation selection experiment was conducted to increase the number of fertile eggs after a single artificial insemination (AI) with pooled Muscovy semen. Reciprocal crossbreeding between Brown Tsaiya LRI-2 (with long duration of fertility) and Pekin L-201 (with white plumage mule ducklings) ducks produced the G0. Then G1 were intercrossed to produce G2 and so on for the following generations. Each female duck was inseminated 3 times, at 26, 29, and 32 weeks of age. The eggs were collected for 14 days from day 2 after AI. Individual data regarding the number of incubated eggs (Ie), the number of fertile eggs at candling at day 7 of incubation (F), the total number of dead embryos (M), the maximum duration of fertility (Dm) and the number of hatched mule ducklings (H) with plumage colour were recorded. The selection criterion was the breeding values of the best linear unbiased prediction animal model for F. The results show high percentage of exhibited heterosis in G2 for traits to improve (19.1% for F and 12.9% for H); F with a value of 5.92 (vs 3.74 in the Pekin L-201) was improved in the G2. Heritabilities were found to be low for Ie ($h^2=0.07{\pm}0.03$) and M ($h^2=0.07{\pm}0.01$), moderately low for Dm ($h^2=0.13{\pm}0.02$), of medium values for H ($h^2=0.20{\pm}0.03$) and F ($h^2=0.23{\pm}0.03$). High and favourable genetic correlations existed between F and Dm ($r_g=0.93$), between F and H ($r_g=0.97$) and between Dm and H ($r_g=0.90$). The selection experiment showed a positive trend for phenotypic values of F (6.38 fertile eggs in G10 of synthetic strain vs 5.59 eggs in G4, and 3.74 eggs in Pekin L-201), with correlated response for increasing H (5.73 ducklings in G10 vs 4.86 in G4, and 3.09 ducklings in Pekin L-201) and maximum duration of the fertile period without increasing the embryo mortality rate. The average predicted genetic response for F was 40% of genetic standard deviation per generation of selection. The mule ducklings' feather colour also was improved. It was concluded that this study provided results for a better understanding of the genetics of the duration of fertility traits in the common female duck bred for mule and that the selection of a synthetic strain was effective method of improvement.

      • KCI등재

        Selection for Duration of Fertility and Mule Duck White Plumage Colour in a Synthetic Strain of Ducks (Anas platyrhynchos)

        H.C. Liu,J.F. Huang,S.R. Lee,H.L. Liu,C.H. Hsieh,C.W. Huang,M.C. Huang,C. Tai,J.P. Poivey,R. Rouvier,Y.S. Cheng 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.5

        A synthetic strain of ducks (Anas platyrhynchos) was developed by introducing genes for long duration of fertility to be used as mother of mule ducklings and a seven-generation selection experiment was conducted to increase the number of fertile eggs after a single artificial insemination (AI) with pooled Muscovy semen. Reciprocal crossbreeding between Brown Tsaiya LRI-2 (with long duration of fertility) and Pekin L-201 (with white plumage mule ducklings) ducks produced the G0. Then G1 were intercrossed to produce G2 and so on for the following generations. Each female duck was inseminated 3 times, at 26, 29, and 32 weeks of age. The eggs were collected for 14 days from day 2 after AI. Individual data regarding the number of incubated eggs (Ie), the number of fertile eggs at candling at day 7 of incubation (F), the total number of dead embryos (M), the maximum duration of fertility (Dm) and the number of hatched mule ducklings (H) with plumage colour were recorded. The selection criterion was the breeding values of the best linear unbiased prediction animal model for F. The results show high percentage of exhibited heterosis in G2 for traits to improve (19.1% for F and 12.9% for H); F with a value of 5.92 (vs 3.74 in the Pekin L-201) was improved in the G2. Heritabilities were found to be low for Ie (h2 = 0.07±0.03) and M (h2 = 0.07±0.01), moderately low for Dm (h2 = 0.13±0.02), of medium values for H (h2 = 0.20±0.03) and F (h2 = 0.23±0.03). High and favourable genetic correlations existed between F and Dm (rg = 0.93), between F and H (rg = 0.97) and between Dm and H (rg = 0.90). The selection experiment showed a positive trend for phenotypic values of F (6.38 fertile eggs in G10 of synthetic strain vs 5.59 eggs in G4, and 3.74 eggs in Pekin L-201), with correlated response for increasing H (5.73 ducklings in G10 vs 4.86 in G4, and 3.09 ducklings in Pekin L-201) and maximum duration of the fertile period without increasing the embryo mortality rate. The average predicted genetic response for F was 40% of genetic standard deviation per generation of selection. The mule ducklings’ feather colour also was improved. It was concluded that this study provided results for a better understanding of the genetics of the duration of fertility traits in the common female duck bred for mule and that the selection of a synthetic strain was effective method of improvement.

      • Response of plasma rotation to resonant magnetic perturbations in J-TEXT tokamak

        Yan, W,Chen, Z Y,Huang, D W,Hu, Q M,Shi, Y J,Ding, Y H,Cheng, Z F,Yang, Z J,Pan, X M,Lee, S G,Tong, R H,Wei, Y N,Dong, Y B IOP 2018 Plasma physics and controlled fusion Vol.60 No.3

        <P>The response of plasma toroidal rotation to the external resonant magnetic perturbations (RMP) has been investigated in Joint Texas Experimental Tokamak (J-TEXT) ohmic heating plasmas. For the J-TEXT’s plasmas without the application of RMP, the core toroidal rotation is in the counter-current direction while the edge rotation is near zero or slightly in the co-current direction. Both static RMP experiments and rotating RMP experiments have been applied to investigate the plasma toroidal rotation. The core toroidal rotation decreases to lower level with static RMP. At the same time, the edge rotation can spin to more than 20 km s<SUP>−1</SUP> in co-current direction. On the other hand, the core plasma rotation can be slowed down or be accelerated with the rotating RMP. When the rotating RMP frequency is higher than mode frequency, the plasma rotation can be accelerated to the rotating RMP frequency. The plasma confinement is improved with high frequency rotating RMP. The plasma rotation is decelerated to the rotating RMP frequency when the rotating RMP frequency is lower than the mode frequency. The plasma confinement also degrades with low frequency rotating RMP.</P>

      • SCIESCOPUSKCI등재

        Effect of Corticosterone Administration on Small Intestinal Weight and Expression of Small Intestinal Nutrient Transporter mRNA of Broiler Chickens

        Hu, X.F.,Guo, Yuming,Huang, B.Y.,Zhang, L.B.,Bun, S.,Liu, D.,Long, F.Y.,Li, J.H.,Yang, X.,Jiao, P. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2

        The effects of corticosterone (CORT) administration on the weight of small intestine and the expression of nutrient transporter mRNA in the small intestine of broiler chickens (Gallus gallus domesticus) were investigated. One hundred and eight sevenday-old birds were randomly divided into two equal groups comprising a control group (CTRL) and an experimental group (CORT). CTRL birds were fed a basal diet and the CORT birds were fed a basal diet containing 30 mg corticosterone/kg from d 8 to 21. At 21 d of age, average daily feed intake (ADFI), serum corticosterone level, small intestinal absolute wet weight and relative weight, and relative abundance of SGLT1, CaBP-D28k, PepT1 mRNA in the duodenum and L-FABP mRNA in the jejunum were determined. The results showed that serum corticosterone level, liver weight and small intestinal relative weight (small intestinal wet weight/body weight) of CORT chickens were about 30.15%, 26.72% and 42.20% higher, respectively, than in the CTRL group (p<0.05). CORT birds had relative mRNA abundance of CaBP-D28k and PepT1 in the duodenum, and L-FABP in the jejunum which was 1.77, 1.37 and 1.94 fold higher, respectively, than in the CTRL group (p<0.05); the relative abundance of SGLT1 was 1.67 fold higher than in the CTRL group (p = 0.097). ADFI, small intestinal wet weight and length in CORT-treated broiler chickens was about 29.11%, 31.12% and 12.35% lower, respectively, than in the CTRL group (p<0.05). In conclusion, corticosterone administration lowered the wet weight but increased the relative weight of the small intestine and the expression of intestinal nutrient transporter mRNA of broiler chickens.

      • Induced quadrupolar singlet ground state of praseodymium in a modulated pyrochlore

        van Duijn, J.,Kim, K. H.,Hur, N.,Ruiz-Bustos, R.,Adroja, D. T.,Bridges, F.,Daoud-Aladine, A.,Fernandez-Alonso, F.,Wen, J. J.,Kearney, V.,Huang, Q. Z.,Cheong, S.-W.,Perring, T. G.,Broholm, C. American Physical Society 2017 Physical Review B Vol.96 No.9

        <P>The complex structure and magnetism of Pr2-xBixRu2O7 was investigated by neutron scattering and extended x-ray absorption fine structure. Pr has an approximate doublet ground state and the first excited state is a singlet. While the B-site (Ru) is well ordered throughout, this is not the case for the A-site (Pr/Bi). A broadened distribution for the Pr-O-2 bond length at low temperature indicates the Pr environment varies from site to site even for x = 0. The environment about the Bi site is highly disordered ostensibly due to the 6s lone pairs on Bi3+. Correspondingly, we find that the non-Kramers doublet ground-state degeneracy, otherwise anticipated for Pr in the pyrochlore structure, is lifted so as to produce a quadrupolar singlet ground statewith a spatially varying energy gap. For x = 0, below TN, the Ru sublattice orders antiferromagnetically, with propagation vector k = (0,0,0) as forY(2)Ru(2)O(7). No ordering associated with the Pr sublattice is observed down to 100 mK. The low-energy magnetic response of Pr2-xBixRu2O7 features a broad spectrum of magnetic excitations associated with inhomogeneous splitting of the Pr quasidoublet ground state. For x = 0 (x = 0.97), the spectrum is temperature dependent (independent). It appears disorder associated with Bi alloying enhances the inhomogeneous Pr crystal-field level splitting so that intersite interactions become irrelevant for x = 0.97. The structural complexity for the A-site may be reflected in the hysteretic uniform magnetization of B-site ruthenium in the Neel phase.</P>

      • KCI등재후보

        Effects of Dietary Fish Oil on the Contents of Eicosapentaenoic Acid and Docosahexaenoic Acid and Sensory Evaluation of the Breast Meat in Mule Ducks

        J. F. Huang,C. C. Huang,M. K. Lai,J. H. Lin,C. H. Lee,T. Y. Wang 아세아·태평양축산학회 2006 Animal Bioscience Vol.19 No.2

        The objectives of this study were to investigate the effects of dietary fish oil inclusion on the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents and organoleptic characteristics of breast meat in mule ducks. Three hundred mule ducks at four weeks of age were randomly assigned to three dietary treatments with five replicate pens in each. One replicate pen had ten males and females each with a total of 100 ducks in each treatment. The diet in the three treatments contained 0, 1.5, and 3.0% fish oil, respectively. Body weights at 4, 6, 8, and 10 weeks of age, and feed efficiency at 4 to 6, 6 to 8, and 8 to 10 weeks of age were recorded. At 10 weeks of age, one male and one female from each replicate were sacrificed for oxidative stability of breast meat and the sacrificed males were employed for the analysis of fatty acids in breast meat and skin. Sensory evaluation of breast meat was also performed. A level of 3.0% fish oil in the diet significantly deteriorated feed efficiency and body weight gain. Dietary fish oil inclusion had a trend of increasing abdominal fat deposition and decreasing the flavor of breast meat. The EPA and DHA contents in the breast meat were higher than those in the breast skin irrespective of oil sources. The EPA and DHA contents in breast meat and breast skin was significantly increased in the 3.0% fish oil group. Although EPA and DHA were not efficiently deposited in the duck meat through dietary fish oil inclusion, this method can still provide a partial supplementation of EPA and DHA.

      • KCI등재

        Synthesis of TiO<sub>2</sub> Nanotubes and Their Sensitivity for Toluene Gas

        Yue, H.Y.,Huang, S.,Guo, E.J.,Wang, L.P.,Kang, F.W.,Yu, Z.M.,Guo, Y.K.,Sun, F.L. The Korean Powder Metallurgy Institute 2011 한국분말재료학회지 (KPMI) Vol.18 No.1

        $TiO_2$ nanopowders with anatase structure were firstly prepared by controlling the pH value of a precursor solution without any heat-treatment at room temperature. The prepared $TiO_2$ nanopowders were hydrothermally treated in 10M NaOH solution at $170^{\circ}C$. Then, the samples were washed in DI water or 0.1M HCl. The $TiO_2$ nanotubes were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM). The gas sensitivity of $TiO_2$ nanotubes for toluene gas was also investigated. The results show that $TiO_2$ nanotubes can be prepared by hydrothermal treatment. The morphology of $TiO_2$ nanotubes prepared by 0.1M HCl washing is destroyed to some extent. $TiO_2$ nanotubes with DI water washing show better sensitivity than that with 0.1M HCl washing.

      • SCIESCOPUSKCI등재

        Population Structure and Biodiversity of Chinese Indigenous Duck Breeds Revealed by 15 Microsatellite Markers

        Liu, W.,Hou, Z.C.,Qu, L.J.,Huang, Y.H.,Yao, J.F.,Li, N.,Yang, N. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.3

        Duck (Anas platyrhynchos) is one of the most important domestic avian species in the world. In the present research, fifteen polymorphic microsatellite markers were used to evaluate the diversity and population structure of 26 Chinese indigenous duck breeds across the country. The Chinese breeds showed high variation with the observed heterozygosity (Ho) ranging from 0.401 (Jinding) to 0.615 (Enshi), and the expected heterozygosity (He) ranging from 0.498 (Jinding) to 0.707 (Jingjiang). In all of the breeds, the values of Ho were significantly lower than those of He, suggesting high selection pressure on these local breeds. AMOVA and Bayesian clustering analysis showed that some breeds had mixed together. The FST value for all breeds was 0.155, indicating medium differentiation of the Chinese indigenous breeds. The FST value also indicated the short domestication history of most of Chinese indigenous ducks and the admixture of these breeds after domestication. Understanding the genetic relationship and structure of these breeds will provide valuable information for further conservation and utilization of the genetic resources in ducks.

      • SCIESCOPUS

        Design of wireless sensor network and its application for structural health monitoring of cable-stayed bridge

        Lin, H.R.,Chen, C.S.,Chen, P.Y.,Tsai, F.J.,Huang, J.D.,Li, J.F.,Lin, C.T.,Wu, W.J. Techno-Press 2010 Smart Structures and Systems, An International Jou Vol.6 No.8

        A low-cost wireless sensor network (WSN) solution with highly expandable super and simple nodes was developed. The super node was designed as a sensing unit as well as a receiving terminal with low energy consumption. The simple node was designed to serve as a cheaper alternative for large-scale deployment. A 12-bit ADC inputs and DAC outputs were reserved for sensor boards to ease the sensing integration. Vibration and thermal field tests of the Chi-Lu Bridge were conducted to evaluate the WSN's performance. Integral acceleration, temperature and tilt sensing modules were constructed to simplify the task of long-term environmental monitoring on this bridge, while a star topology was used to avoid collisions and reduce power consumption. We showed that, given sufficient power and additional power amplifier, the WSN can successfully be active for more than 7 days and satisfy the half bridge 120-meter transmission requirement. The time and frequency responses of cables shocked by external force and temperature variations around cables in one day were recorded and analyzed. Finally, guidelines on power characterization of the WSN platform and selection of acceleration sensors for structural health monitoring applications were given.

      • SCISCIESCOPUS

        Identification and evaluation of potent Middle East respiratory syndrome coronavirus (MERS-CoV) 3CL<sup>Pro</sup> inhibitors

        Kumar, V.,Shin, J.S.,Shie, J.J.,Ku, K.B.,Kim, C.,Go, Y.Y.,Huang, K.F.,Kim, M.,Liang, P.H. Elsevier/North-Holland 2017 ANTIVIRAL RESEARCH Vol.141 No.-

        <P>Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe acute respiratory illness with fever, cough and shortness of breath. Up to date, it has resulted in 1826 human infections, including 649 deaths. Analogous to picornavirus 3C protease (3C(PrO)), 3C-like protease (3CL(PrO)) is critical for initiation of the MERS-CoV replication cycle and is thus regarded as a validated drug target. As presented here, our peptidomimetic inhibitors of enterovirus 3C(PrO) (6b. 6c and 6d) inhibited 3CLPr0 of MERS-CoV and severe acute respiratory syndrome coronavirus (SARS-CoV) with IC50 values ranging from 1.7 to 4.7 M and from 0.2 to 0.7 M, respectively. In MERS-CoV-infected cells, the inhibitors showed antiviral activity with EC50 values ranging from 0.6 to 1.4 1.1M, by downregulating the viral protein production in cells as well as reducing secretion of infectious viral particles into culture supernatants. They also suppressed other cc and 8-CoVs from human and feline origin. These compounds exhibited good selectivity index (over 70 against MERS-CoV) and could lead to the development of broad-spectrum antiviral drugs against emerging CoVs and picornaviruses. (C) 2017 Elsevier B.V. All rights reserved.</P>

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