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      • KCI등재

        Diagnostic Equipment for the TEMP-4M Generator of High-current Pulsed Ion Beams

        Yulia I. Isakova 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.61

        The results of calibration and optimization of the diagnostic equipment for a gigawatt power pulsed ion beam accelerator are presented. The study was performed using the TEMP-4M accelerator set in a mode of double pulse formation. The first pulse is negative (300 - 600 ns, 100 - 150 kV) followed by the second positive pulse (80 ns, 250 - 300 kV). The ion current density is 30 - 300 A/cm<sup>2</sup> (for different designs of diodes), the ion energy is 250 - 300 keV, and the beam is composed of protons and carbon ions. The calibration of the diagnostic equipment shows that it correctly reflects the accelerator operation in short circuit mode (U = 50 - 60 kV), when operating with a resistive load up to 10 ?(200?00 kV) and when operating with the diode. A technique based on time-of-flight (TOF) method for a quick determination of beam composition (ion type and degree of ionization) was tested. The method allows for the determination of absolute values of the ion current density and the energy spectrum for each ion type with an accuracy of ±10%.

      • Designing high performance all-small-molecule solar cells with non-fullerene acceptors: comprehensive studies on photoexcitation dynamics and charge separation kinetics

        Shi, Junqing,Isakova, Anna,Abudulimu, Abasi,van den Berg, Marius,Kwon, Oh Kyu,Meixner, Alfred J.,Park, Soo Young,Zhang, Dai,Gierschner, Johannes,,er, Larry The Royal Society of Chemistry 2018 ENERGY AND ENVIRONMENTAL SCIENCE Vol.11 No.1

        <P>Solution-processable all-small-molecule organic solar cells (OSCs) have shown dramatic progress in improving stability and photovoltaic efficiency. However, knowledge of photoexcitation dynamics in this novel class of materials is very limited. To fully exploit the design capacities inherent in small molecule chemistry, the elementary processes and branching yields must be known in detail. Here, we present a combined computational-experimental study of photoexcitation dynamics of a prototypical all-small-molecule photovoltaic blend, p-DTS(FBTTh2)2 as a donor and NIDCS-MO as an acceptor. Femtosecond spectroscopy data show that excitonic coupling is small and that the charge transfer states are localized, at first glance contradicting the high internal quantum efficiency (IQE) and open circuit voltage (<I>V</I>OC) of this material. A target analysis of the femtosecond spectra yields exciton dissociation rates of 1/(25 ps) and 1/(100 ps) for the as-deposited and annealed blend, respectively. These rates are far slower than in typical polymer based organic solar cells. Still, internal quantum yields are high because parasitic quenching processes are found to be even slower. In the framework of semiclassical Marcus theory, we demonstrate that our system shows near-optimum energy conversion and charge separation yields, due to negligible activation energy for charge generation but high activation energy for charge recombination, allowing enough time to separate localized charge transfer states. We thus justify both the high internal quantum yields and the high open circuit voltage found in this system. Finally, we predict that highly efficient and stable low-optical bandgap systems can be realized by reducing the electronic coupling between the donor and acceptor.</P>

      • KCI등재

        Energy Status of Neurospora crassa Mutant nap in Relation to Accumulation of Carotenoids

        TatyanaA.Belozerskaya,TatyanaV.Potapova,ElenaP.Isakova,EugeneI.Shurubor,LudmilaV.Savel'eva,RenataA.Zvyagilskaya 한국미생물학회 2003 The journal of microbiology Vol.41 No.1

        N. crassa mutant strain nap showed reduced growth rate, decreased electric membrane potential, and elevated intracellular ATP content in comparison to the wild type. Blue light induced a hyperpolarization of the membrane potential in both strains. The analysis of oxidative and phosphorylation activities of mitochondria isolated from the two strains has revealed that nap utilized more efficient oxidative pathways. The higher intracellular ATP content in the nap was presumably due to impaired transport systems of the plasma membrane, and to a lesser extent to the functioning of the fully competent respiratory chain. The excess ATP possibly accounts for carotenoid accumulation in the mutant.

      • KCI등재

        Expression System for Yarrowia lipolytica Based on a Promoter of the Mitochondrial Potential-dependent Porin VDAC Gene

        Ekaterina Yu Epova,Maria V. Balovneva,Elena P. Isakova,Yuliya K. Kudykina,Marina V. Zylkova,Yulia I. Deryabina,Alexei B. Shevelev 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.3

        In this study, we designed an expression system for the Y. lipolytica yeast, which can be fully efficient in media with non-standard industrial ingredients. Previously, we reported that the mitochondrial Voltage Dependent Anion Channel (VDAC) was a major protein overproduced in the Yarrowia lipolytica yeast in an alkaline (pH = 9.0) culture medium. In this study, the VDAC promoter was cloned and tested using a reporter system based on the LacZ gene. Naturally, the VDAC gene contains an intron located just within the ATG translation initiation codon. The VDAC promoter V2 variant with the intron and V3 variant without the intron were studied. The VDAC-driven clones exhibited high variability of the expression profile. Some clones were more active than the clones induced by the artificial hp4d promoter, when grown in both complete and low-cost industrial ingredient (10% fish and sunflower meal) containing media. The new expression system may greatly expand the range of recombinant producers of feed enzymes and some other types of fodder additives in the Y. lipolytica yeast, appropriate for assimilation of low-cost and non-standard raw material.

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