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콩 Cyst 선충 Race 5에 대한 저항성 QTL 탐색
In Soo Choi,Yong Chul Kim,Sung Man Kim,Chung Yeol Lee,Hyean Cheal Park,Halina T. Skorupska 韓國作物學會 1997 Korean journal of crop science Vol.42 No.6
콩 품종 Essex와 PI 437654간 교잡 후 F2 유래 F3 계통들을 재료로 하여 작성된 RAPD 유전자지도상에 cyst 선충 race 5에 대한 저항성 QTLs 분석을 실시한 바 결과를 요약하면 다음과 같다. 1. 회귀분석 결과 26개의 marker들(22 RAPD, 4 RFLP)에서 cyst 선충 race 5 저항성 반응에 대한 유의성이 인정되었다. 2. MAPMAKER /QTL 분석 결과2개의 저항성 QTL들이 탐색되었는데, 이 QTL들은 2개의 linkage groups(LGC-20와 Group 2)에 위치하였다. 3. 탐색된 2개의 QTL들 중 1개는 우성유전, ?고 나머지 하나는 열성유전양상을 나타내었다. 4. 콩 cyst 선충 race 5의 저항성에 대한 유의성이 인정되는 5개의 marker들간 상호작용을 알아보기 위한 다중회귀분석 결과 총 26개의 조합들 중 4개의 marker들(E023 , G101 , W03, pK418C)로 구성된 조합에서 가장 높은 표리적 변이의 값(35.2%)을 나타내었다. The objectives of this study were; (1) to identify and localize QTLs for resistance to soybean cyst nematode(SCN) race 5 on RAPD map, (2) to idntify the magnitude and mode of inheritance for each QTL, and (3) to identify the best combinations of QTLs for resistance to SCN race 5. Based on the univariate regression analysis, we detected 26 markers(22 RAPD and 4 RFLP) which showed significant association(P<0.05) with resistance to SCN race 5. From MAPMAKER /QTL analysis, we identified two regions (LGC-20 and Group 2) for resistance to SCN race 5. The QTL that was localized at 8.0 cM from pK418C on LGC-20 showed a recessive mode of inheritance and the QTL that was localized between W03 and E023 on Group 2 showed a dominant mode of inheritance. Two pairs of flanking markers (E023 and W03, pK418C and pK418E1 ) and one unlinked RAPD marker, G101 were used for multiple regression analysis. Marker combination which was composed of 4 markers, E023 , G101 , W03, and pK418E1 , explained the highest amount of phenotypic variation by SCN (35.2%). Further research for the identification of QTLs for resistance to SCN race 5 to explain larger portion of phenotypic variation is needed.
In-soo Choi,Han-sang Yoo,Ellen W. Collisson 대한수의학회 2000 대한수의학회 학술대회발표집 Vol.2000 No.-
Feline immunodeficiency virus (FIV) causes AIDS-like signs of illness in infected cats. Con-A stimulated peripheral blood mononuclear cells (PBMC) from chronically FIV-PPR infected cats readily expressed FIV. In contrast, when PBMC from these animals were stimulated with irradiated autologous antigen presenting cells (APC), as much as a 10-fold decrease in virus production was observed. In addition to FIV-specific cytotoxic T lymphocytes, anti-FIV activity was demonstrated from T lymphocytes cultured with these irradiated APC. The FIV-suppressive activity in supernatants was induced from APC-stimulated PBMC of either FIV-infected or uninfected cats but not from Con-A stimulated PBMC. The anti-FIV factor(s) suppressed FIV-PPR replication in both autologous and heterologous feline PBMC. The anti-FIV activity was dose-dependent, heat stable and cross-reactive, and demonstrated cell specificity. It was also demonstrated that the anti-FIV activity originated from CD8<sup>+</sup>T lymphocytes and that the suppression was mediated by a noncytolytic mechanism.
Current Researches and Prospects of Soybean Cyst Nematode
Insoo Choi,Namsook Park,Eulsoo Yun,Donggeun Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10
Plant-parasitic nematodes causes yield losses of more than $100 million per year in the world. Among them, soybeancyst nematode (Heterodera glycines Ichinohe) causes the greatest yield loss to soybean compared to any other pest worldwide.SCN causes yield reductions by feeding on plant nutrients, retarding root growth and inhibiting Bradyrhizobium japonicum(Kirchner) Buchanan nodulation. The primary methods for controlling SCN include planting resistant cultivars and rotationwith nonhost crop. In order to control SCN, we should be breeding resistant soybean cultivars which has resistant genefor this pest. The research that can minimize the damage of the soybean yield is still insufficient, comprehensive andsystematic research is urgently required. Current researches in our lab are discussed.
Han Sang Yoo,InSooChoi,JongManKim,WonHur,유한상 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.1
Swine respiratory diseases induce severe conomicloses in the swine industry worldwide. Severalmethods have ben developed and applied to controlthese diseases. However, there are stil problems ofdisease control in the swine industry. Recently, egyolk antibodies have ben found to offer several ad-vantages for disease control in animals and humans.In a previous tudy (24), antibodies to severaldeveloped. However, several problems remained,especialy in terms of reduced laying rates. Therefore,experimental vacines were reformulated with variousbacterial antigens of the swine respiratory diseases.After imunizing hens with the antigens, antibodyprofiles and other effects including laying rates wereinvestigated and compared to those of the previousstudy.Profiles of antibody titers were very similar withthose of the previous study. However, side fects,such as depression, weakness, reduction of layinglaying rates were increased in hens injected withcertain experimental vacines. In particular, layingrates of hens injected with vaccines against atrophicrhinitis were increased up to 84% by injecting avaccine composed of only the DNTs of B. bron-chiseptica and P. multocida D:4. Efficacies of thevaccines against swine pneumonic pasteurelosis andpleuropneumonia were very similar with those of theprevious study. These results uggest hat newvacines could be fective in the production of egyolk antibodies against the causative agents of swine*Adress: Department of Infectious Disease, Colege of VeterinaryMedicine and School of Agricultural Biotechnology, Seoul NationalUniversity, Suwon, 441-744 KoreaTel: +82-31-290-2737, Fax: +82-31-290-2737E.mail: yoohs@plaza.snu.ac.krrespiratory diseases.Key words ; IgY, Swine, Respiratory Diseases.