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      • Disorders of Small and Large Intestine : Expression Pattern Of E-And N-Cadherin In Colorectal Adenocarcinoma With Liver Metastasis

        ( Hyeong Cheon Park ), ( Wan Sik Lee ), ( Chang Hwan Park ), ( Hyun Soo Kim ), ( Sung Kyu Choi ), ( Jong Sun Rew ), ( Jae Hyuk Lee ), ( Seok Lee ), ( Sang Min Yum ), ( Ho In Whang ), ( Kyoung Won Yoon ), ( Suck ) 대한소화기학회 2007 SIDDS Vol.9 No.-

        Background Aims: Colorectal carcinoma (CRC) is known to be metastasized to the liver frequently and is the major cause of cancer-related death. Recent studies have identified several biomolecular alterations that influence prognosis after resection of liver matastases, including treatment-related and clinicopathologic factors. However, the relationship between the expression pattern of E- & N-cadherin and liver metastasis of CRC is poorly understood. Methods: The present study included 45 patients who underwent hepatic resection for colorectal hepatic metastasis. Immunohistochemical analysis of the expression of E- & N-cadherin protein was carried out for evaluating the metastatic potential of CRC. The methylation status of the E- & N-cadherin promoter was investigated by utilizing the methylation-specific polymerase chain reaction (MSP) assay in 45 patients. The correlation between E- & N-cadherin expression and clinicopathologic parameters was also analysed. Results: Abnormal E-cadherin expressions observed 42.2% (19/45) in primary cancers and 46.7% (21/45) in metastatic tumors. Abnormal N-cadherin expressions were noted 71.1% (32/45) in primary cancers and 82.2% (37/45) in metastatic tumors. Methylated alleles for E-cadherin presented 73.3% (33/45) in primary cancers and 64.4% (29/45) in metastatic tumors. Methylated alleles for N-cadherin presented 11.1%(5/45) in primary cancers and 8.9% (4/45) in metastatic tumors. Abnormal E-cadherin expression was significantly associated with hypermethylation of E-cadherin in primary CRC (P=0.036). Recurrence rate was significantly associated with liver mass size (>3 cm) and abnormal E-cadherin expression in liver (p=0.02). Survival rate was significantly associated with liver metastasis number (multiple)(p=0.01). Conclusions: Abnormal expression of E-cadherin was significantly associated with E-cadherin promoter methylation in CRC. Liver metastatic mass size and metastasis number were significantly associated with survival rate but E-cadherin, N-cadherin and methylation state in liver were not significantly associated with survival rate.

      • KCI등재

        Effects of Concrete and Wood Building Environments on Pregnant Dams and Embryo-Fetal Development in Rats

        In-Sik,Shin,Sung-Hwan,Kim,Jeong-Hyeon,Lim,Jong-Chan,Lee,Na-Hyeong,Park,Dong-Ho,Shin,Changjong,Moon,Sung-Ho,Kim,Jong-Choon,Kim 한국독성학회 2009 Toxicological Research Vol.25 No.4

        We have recently reported that the continuous exposure of rats to a concrete building environment under cool temperatures had adverse effects on general health parameters and embryo-fetal development. This study examined to compare the potential effects of concrete and wood building environments on pregnant dams and embryo-fetal development in rats. Groups of 10 mated females were exposed to polycarbonate (control), concrete, or wood cages from gestational days (GD) 0 to 20 under cool temperatures (11.9~12.3℃). All the females underwent a caesarean section on GD 20, and their fetuses were examined for any morphological abnormalities. The temperatures in the cages were similar in all groups but the relative humidity in the concrete and wood groups were higher than in the control group. The concentration of volatile organic compounds in the wood group was higher than in the control group. In the concrete group, maternal effects manifested as an increase in the incidence of clinical signs, a lower body weight, and a decrease in the thymus and ovary weights. Developmental effects included increased post-implantation loss and decreased litter size. Infrared thermal analysis showed that the skin temperature of the rats in the concrete group was lower than that in the control group. In contrast, there were no exposure-related adverse effects on the maternal and developmental parameters in the wood group. Overall, the exposure of pregnant rats to a concrete building environment under cool temperatures has adverse effects on the clinical signs, body weight, skin temperature, organ weight, and embryo-fetal development. On the other hand, exposure to a wood building environment does not have any adverse effects in rats.

      • KCI등재
      • Variability in Drug Interaction According to Genetic Polymorph isms in Drug Metabolizing Enzymes

        In-Jin,Jang,Kyung-Sang,Yu,Joo-Youn,Cho,Jae-Yong,Chung,Jung-Ryul,Kim,Hyeong-Seok,Lim,Sang-Goo,Shin 한국환경성돌연변이발암원학회 2004 한국환경성돌연변이·발암원학회지 Vol.24 No.1

        There are significant differences in the extent of drug interactions between subjects. The influence of the genetic make up of drug metabolizing enzyme activities (CYP3A5, CYP2C19 and UDP-gIucuronosyl transferase) on the pharmacokinetic drug interaction potential were studied in vivo. Nineteen healthy volunteers were grouped with regard to the CYP3A5*3 allele, into homozygous wild-type (CYP3A5*1/*1, n=6), heterozygous (CYP3A5*1/*3, n=6), and homozygous variant-type (CYP3A5*3/*3, n=7) subject groups. The pharmacokinetic profile of intravenous midazolam was characterized before and after itraconazole administration (200 mg once daily for 4 days), and also following rifampin pretreatment (600 mg once daily for 10 days), with a washout period of 2 weeks in between. For omeprazole and moclobemide pharmacokinetic interaction study 16 healthy volunteers were recruited. The volunteer group comprised 8 extensive metabolizers and 8 poor metabolizers of CYP2C19, which was confirmed by genotyping. Subjects were randomly allocated into two sequence groups, and a single-blind, placebo-controlled, two-period crossover study was performed. In study Ⅰ, a placebo was orally administered for 7 days. On the eighth morning, 300 mg of moclobemide and 40 mg of placebo were coadministered with 200 mL of water, and a pharmacokinetic study was performed. During study Ⅱ, 40 mg of omeprazole was given each morning instead of placebo, and pharmacokinetic studies were performed on the first and eighth day with 300 mg of moclobemide coadministration. In the UGT study pharmacokinetics and dynamics of 2 mg intravenous lorazepam were evaluated before and after rifampin pretreatment (600 mg once daily for 10 days), with a washout period of 2 weeks in between. The subjective and objective pharmacodynamic tests were done before and 1, 2, 4, 6, 8, and 12 hrs after lorazepam administration. The pharmacokinetic profiles of midazolam and of its hydroxy metabolites did not show differences between the genotype groups under basal and induced metabolic conditions. However, during the inhibited metabolic state, the CYP3A5*3/*3 group showed a greater decrease in systemic clearance than the CYP3A5*1/*1 group (8.5±3.8 L/h/70 kg vs. 13.5± 2.7 L/h/70 kg, P=0.027). The l'-hydroxymidazolam to midazolam AUC ratio was also significantly lower in the CYP3A5*3/*3 group (0.58±0.35, vs. 1.09±0.37 for the homozygous wild-type group, P=0.026). The inhibition of moclo-bemide metabolism was significant in extensive metabolizers even after a single dose of omeprazole. After daily administration of omeprazole for 1 week, the pharmacokinetic parameters of moclobemide and its metabolites in extensive metabolizers changed to values similar to those in poor metabolizers. In poor meta-bolizers, no remarkable changes in the pharmacokinetic parameters were observed. The area under the time-effect curves of visual analog scale(VAS), choice reaction time, and continuous line tracking test results of lorazepam was reduced by 20%, 7%, 23% respectively in induced state, and in spite of large interindividual variablilty, significant statistical difference was shown in VAS (repeated measures ANOVA, p=0.0027).

      • Variability in Drug Interaction According to Genetic Polymorphisms in Drug Metabolizing Enzymes

        In-Jin,Jang,Kyung-Song,Yu,Joo-Youn,Cho,Joe-Yong,Chung,Jung-Ryul,Kim,Hyeong-Seok,Lim,Sang-Goo,Shin 한국환경성돌연변이발암원학회 2003 한국환경성돌연변이·발암원학회지 Vol.23 No.4

        There are significant differences in the extent of drug interactions between subjects. The influence of the genetic make up of drug metabolizing enzyme activities (CYP3A5, CYP2C19 and UDP-glucuronosyl trans-ferase) on the pharmacokinetic drug interaction potential were studied in vivo. Nineteen healthy volunteers were grouped with regard to the CYP3A5*3 allele, into homozygous wild-type (CYP3A5*1/*1, n=6), heterozygous (CYP3A5*1/*3, n=6), and homozygous variant-type (CYP3A5*3/*3, n=7) subject groups. The pharmacokinetic profile of intravenous midazolam was characterized before and after itraconazole administration (200 mg once daily for 4 days), and also following rifampin pretreatment (600 mg once daily for 10 days), with a washout period of 2 weeks jn between. For omeprazole and moclo-bemide pharmacokinetic interaction study 16 healthy volunteers were recruited. The volunteer group comprised 8 extensive metabolizers and 8 poor metabolizers or CYP2CI9, which was confirmed by genotyping. Subjects were randomly allocated into two sequence groups, and a single-blind, placebo-controlled, two-period crossover study was performed. In study I, a placebo was orally administered for 7 days. On the eighth morning, 300 mg of moclobemide and 40 mg of placebo were coadministered with 200 mL of water, and a pharmacokinetic study was performed. During study Ⅱ, 40 mg of omeprazole was given each morning instead of placebo, and pharmacokinetic studies were performed on the first and eighth day with 300 mg of moclobemide coadministration. In the UGT study pharmacokinetics and dynamics of 2 mg intravenous lorazepam were evaluated before and after rifampin pretreatment (600 mg once daily for 10 days), with a washout period of 2 weeks in between. The subjective and objective pharmacodynamic tests were done before and I, 2, 4, 6, 8, and 12 hours after lorazepam administration. The pharmacokinetic profiles of midazolam and of its hydroxy metabolites did not show differences between the genotype groups under basal and induced metabolic conditions. However, during the inhibited metabolic state, the CYP3A5*3/*3 group showed a greater decrease in systemic clearance than the CYP3A5*1/*1 group (8.5 ± 3.8 L/h/70 kg vs. 13.5 ± 2.7 L/h/70 kg, P=0.027). The l'-hydroxymidazolam to midazolam AUC ratio was also significantly lower in the CYP3A5*3/*3 group (0.58±0.35, vs. 1.09±0.37 for the homozygous wild-type group, P=0.026). The inhibition of moclo-bemide metabolism was significant in extensive meta-bolizers even after a single dose of omeprazole. After daily administration of omeprazole for 1 week, the pharmacokinetic parameters of moclobemide and its metabolites in extensive metabolizers changed to values similar to those in poor metaholizers. In poor meta-bolizers, no remarkable changes in the pharmacokinetic parameters were observed. The area under the time-effect curves of visual analog scale(VAS), choice reaction time, and continuous line tracking test results of lorazepam was reduced by 20%, 7%, 23% respectively in induced state, and in spite of large interindividual variablilty, significant statistical difference was shown in VAS (repeated measures ANOVA, p=0.0027).

      • Variability in Drug Interaction According to Genetic Polymorphisms in Drug Metabolizing Enzymes

        Jang,,In-Jin,Yu,,Kyung-Sang,Cho,,Joo-Youn,Chung,,Jae-Yong,Kim,,Jung-Ryul,Lim,,Hyeong-Seok,Shin,,Sang-Goo Korean Environmental Mutagen Society 2004 한국환경성돌연변이·발암원학회지 Vol.24 No.1

        There are significant differences in the extent of drug interactions between subjects. The influence of the genetic make up of drug metabolizing enzyme activities (CYP3A5, CYP2C19 and UDP-glucuronosyl transferase) on the pharmacokinetic drug interaction potential were studied in vivo. Nineteen healthy volunteers were grouped with regard to the $CYP3A5^{*}3$ allele, into homozygous wild-type (CYP3A5^{*}1/1^{*}1$, n=6), heterozygous $(CYP3A5^{*}1/^{*}3$, n=6), and homozygous variant-type $(CYP3A5^{*}3/^{*}3$, n=7) subject groups. The pharmacokinetic profile of intravenous midazolam was characterized before and after itraconazole administration (200 mg once daily for 4 days), and also following rifampin pretreatment (600 mg once daily for 10 days), with a washout period of 2 weeks in between. For omeprazole and moclobemide pharmacokinetic interaction study 16 healthy volunteers were recruited. The volunteer group comprised 8 extensive metabolizers and 8 poor metabolizers of CYP2C19, which was confirmed by genotyping. Subjects were randomly allocated into two sequence groups, and a single-blind, placebo-controlled, two-period crossover study was performed. In study I, a placebo was orally administered for 7 days. On the eighth morning, 300 mg of moclobemide and 40 mg of placebo were coadministered with 200 mL of water, and a pharmacokinetic study was performed. During study n, 40 mg of omeprazole was given each morning instead of placebo, and pharmacokinetic studies were performed on the first and eighth day with 300 mg of moclobemide coadministration. In the UGT study pharmacokinetics and dynamics of 2 mg intravenous lorazepam were evaluated before and after rifampin pretreatment (600 mg once daily for 10 days), with a washout period of 2 weeks in between. The subjective and objective pharmacodynamic tests were done before and 1, 2, 4, 6, 8, and 12 hrs after lorazepam administration. The pharmacokinetic profiles of midazolam and of its hydroxy metabolites did not show differences between the genotype groups under basal and induced metabolic conditions. However, during the inhibited metabolic state, the $CYP3A5^{*}3/^{*}3$ group showed a greater decrease in systemic clearance than the $CYP3A5^{*}1/^{*}1$ group $(8.5\pm3.8$ L/h/70 kg vs. $13.5\pm2.7$ L/h/70 kg, P=0.027). The 1'-hydroxymidazolam to midazolam AUC ratio was also significantly lower in the $CYP3A5^{*}3/^{*}3$, group $(0.58\pm0.35,$ vs. $1.09\pm0.37$ for the homozygous wild-type group, P=0.026). The inhibition of moclo-bemide metabolism was significant in extensive metabolizers even after a single dose of omeprazole. After daily administration of omeprazole for 1 week, the pharmacokinetic parameters of moclobemide and its metabolites in extensive metabolizers changed to values similar to those in poor metabolizers. In poor meta-bolizers, no remarkable changes in the pharmacokinetic parameters were observed. The area under the time-effect curves of visual analog scale(VAS), choice reaction time, and continuous line tracking test results of lorazepam was reduced by 20%, 7%, 23% respectively in induced state, and in spite of large interindividual variablity, significant statistical difference was shown in VAS(repeated measures ANOVA, p=0.0027).

      • KCI등재후보

        ORIGINAL PAPER : Effects of exhaustive exercise on ER Stress of skeletal muscle and adipose tissue in rats

        ( Dae Hyeong In ), ( Sang Koo Woo ), ( Ki Hoon Kim ) 한국운동영양학회 2013 Journal of exercise nutrition & biochemistry Vol.17 No.2

        The purpose of this study was to identify the effects of gene expression of endoplasmic reticulum (ER) stress in skeletal muscle and adipose tissue on acute exhaustive exercise. Thirty-five Sparague Dawley male rats were divided into a control group (CON, n = 7) and a exhaustive exercise group (n = 28), immediately after exhaustive exercise group (n = 7), after 30 minutes exhaustive exercise group (n = 7), after 60 minutes exhaustive exercise group (n = 7), after 180 minutes exhaustive exercise group (n = 7). As a result, changes in the composition of the blood serum triglyceride concentration increased significantly in immediately after exhaustive exercise group, On the contrary, blood glucose showed a significantly decreased (p < .05). Homeostasis of energy metabolism due to exhaustive exercise as a result of the mechanism of action of skeletal muscle in the glycogenolysis and absorption, which indicates that the process of means. On the other hand, a result of examining changes in endoplasmic reticulum stress-related proteins in skeletal muscle and adipose tissue, JNK1 except in skeletal muscle BiP, ATF4, CHOP, GRP78 mRNA increased significantly immediately after exercise, and after 30 minutes returned to normal levels that could be confirmed (p < .05). BiP mRNA in adipose tissue show a similar pattern and skeletal muscle increased significantly immediately after exercise, but other changes in the specificity of the endoplasmic reticulum stress-related proteins also did not appear. In conclusion, Exercise applies and exercise training duration and exercise intensity as well as research on the interaction of the endoplasmic reticulum stress-related genes should be study continuously, to be more clear.

      • KCI등재SCOPUS

        Infection Status of Estuarine Fish and Oysters with Intestinal Fluke Metacercariae in Muan-gun,Jeollanam-do, Korea

        Shin-Hyeong,Cho,In-Sang,Kim,Eun-Jung,Hwang,Tong-Soo,Kim,Byoung-Kuk,Na,Woon-Mok,Sohn 대한기생충학열대의학회 2012 The Korean Journal of Parasitology Vol.50 No.3

        The source of human infection with intestinal flukes was surveyed in estuarine fishes, including the dotted gizzard shad, common sea bass, common blackish goby, redlip mullet, black sea bream, and oyster collected from Muan-gun, Jeollanam-do, Korea during August and September 2007. Collected fishes and oysters were artificially digested in pepsin-HCl solution and examined under a stereomicroscope. In 36 shads (Konosirus punctatus) and 20 basses (Lateolabrax japonicus) examined, Heterophyopsis continua metacercariae were found in 58.3% and 100%, and their average numbers were 12.0 and 6.3 per infected fish, respectively. In 34 gobies (Acanthogobius flavimanus) examined, metacercariae of H. continua were detected in 79.4%, Stictodora lari in 97.1%, and Acanthotrema felis in 92.1%, and their average numbers were 45.8, 189.3, and 235.3 per infected fish, respectively. In 37 redlip mullets (Chelon haematocheilus), Heterophyes nocens metacercariae were found in 56.8%, Pygidiopsis summa in 94.6%, and Stictodora fuscata in 45.9%, and the average metacercarial densities were 17.4, 31.3, and 35.1 per infected fish, respectively. In 30 black sea breams (Acanthopagrus schlegeli) and 45 oysters (Crassostrea gigas) examined, no metacercariae were detected. From the above results, it has been confirmed that the dotted gizzard shad, common sea bass, common blackish goby, and redlip mullet from Muan-gun, Jeollanam-do, Korea are infected with the metacercariae of heterophyid flukes.

      • SCOPUSKCI등재

        Effects of Concrete and Wood Building Environments on Pregnant Dams and Embryo-Fetal Development in Rats

        Shin,,In-Sik,Kim,,Sung-Hwan,Lim,,Jeong-Hyeon,Lee,,Jong-Chan,Park,,Na-Hyeong,Shin,,Dong-Ho,Moon,,Chang-Jong,Kim,,Sung-Ho,Kim,,Jong-Choon The Korean Society of Toxicology Korea Environment 2009 Toxicological Research Vol.26 No.4

        We have recently reported that the continuous exposure of rats to a concrete building environment under cool temperatures had adverse effects on general health parameters and embryo-fetal development. This study examined to compare the potential effects of concrete and wood building environments on pregnant dams and embryo-fetal development in rats. Groups of 10 mated females were exposed to polycarbonate (control), concrete, or wood cages from gestational days (GD) 0 to 20 under cool temperatures $(11.9\sim12.3^{\circ}C)$. All the females underwent a caesarean section on GD 20, and their fetuses were examined for any morphological abnormalities. The temperatures in the cages were similar in all groups but the relative humidity in the concrete and wood groups were higher than in the control group. The concentration of volatile organic compounds in the wood group was higher than in the control group. In the concrete group, maternal effects manifested as an increase in the incidence of clinical signs, a lower body weight, and a decrease in the thymus and ovary weights. Developmental effects included increased post-implantation loss and decreased litter size. Infrared thermal analysis showed that the skin temperature of the rats in the concrete group was lower than that in the control group. In contrast, there were no exposure-related adverse effects on the maternal and developmental parameters in the wood group. Overall, the exposure of pregnant rats to a concrete building environment under cool temperatures has adverse effects on the clinical signs, body weight, skin temperature, organ weight, and embryo-fetal development. On the other hand, exposure to a wood building environment does not have any adverse effects in rats.

      • KCI등재

        영화 속 인공지능의 역할 변화에 대한 연구

        인수형(In, Su-hyeong) 한국영화학회 2017 영화연구 Vol.0 No.72

        신기한 볼거리로 출발한 영화는 이후 과학기술의 비약적 발전과 더불어 오늘날 첨단 영화예술로 성장했다. 현대 영화에서 표현력은 더욱 풍부해지고 현실감은 더욱 극대화되었다. 관객들은 상상으로만 가능했던 것을 스크린에 현실화시키는 영화에 열광했으며, 과학기술을 전면에 내세운 SF 장르의 영화나 블록버스터에서 나타나는 시각적 충격에 감탄하고 있다. 그러나 최근에 등장한 일련의 영화들은 과학기술 자체를 단순히 시각적 충격의 극대화를 위한 도구로 사용하는 것을 넘어서 아예 그것을 영화의 내러티브를 이끌어가는 주인공으로 등장시키고 있다. 요컨대 과학기술 자체가 더 이상 영화 속 조연이 아니라 인간처럼 사고를 하는 하나의 인격체가 되어 드라마를 이끌어가는 주연으로 등장하기 시작한 것이다. 그것은 다름 아닌 인공지능(AI: Artificial Intelligence)인데, 오늘날 영화에서는 이 인공지능이 인간처럼 감정을 가진 인격체로 등장하여 인간보다 더 인간적인 역할을 떠맡고 있는 것이다. 본 논문에서는 이러한 부분에 초점을 맞추어 만들어진 <로맨틱 컴퓨터>(Electric Dreams)와 <그녀>(her)라는 두 편의 텍스트를 중심으로 하여 극중 역할의 변화에 대해서 살펴보고자 한다. 이들 작품들은 인공지능을 지닌 컴퓨터가 인간이 가지는 감정을 이해하고 스스로 행동할 수 있게 된다는 설정을 바탕으로 하고 있다는 공통점이 있다. 또 하나의 공통점은 이 영화들이 SF 장르의 영화가 아니라 일반적인 러브스토리를 다룬 로맨틱 코미디라는 점이다. 이들 영화들에서 인공지능은 인격을 갖춘 하나의 주체로서 내러티브를 이끌어가는 핵심적 역할을 한다. 특히 인공지능이 보다 진보된 운영체계(OS)의 형태로 나타난 <그녀>(her)에서 인공지능은 단순한 기계가 아닌 사람처럼 배우고 판단하는 딥러닝(Deep Learning)을 하는 운영체계이자 여주인공으로서 등장한다. 영화예술의 표현력을 증대하기 위한 도구였던 과학기술 자체가 극중 주인공으로 등장하여 인간과 당당히 경쟁을 한다는 설정은 매우 의미 있는 시도라고 여겨진다. 극중 다양한 형태로 등장하는 인공지능의 존재 자체가 인류의 미래에 대한 강력한 메시지를 던져주고 있기 때문이다. 영화의 내러티브 속으로 들어온 과학기술 자체, 또는 고도로 프로그래밍이 된 인공지능이 진짜 인간들과 상호 소통하는 과정을 통해서 최첨단 과학문명이 이룩할 인류의 미래를 선취(先取)할 수 있기 때문이다. 그리하여 본 논문에서는 영화의 내러티브를 실질적으로 이끌어가는 인공지능 캐릭터의 역할 변화와 그 의미에 대해서 살펴보고자 한다. The film, which started with science and technology, showed a cinematograph at Grand Café in Paris, France on December 28, 1895, and it has grown along with the development of science and technology. In addition to maximizing expressions and realism, the movie is also filled with imagination. The audience is enthusiastic and admires the visual impact of films and blockbusters in SF genres that put science and technology at the forefront. However, the recently emerging film has emerged as a main character in which science and technology play a role in the film beyond the instrumental function of distinguishing genres in movies and maximizing visual impact, and leading important narratives. Technology is no longer an actor in the film, but a human person who is thinking in human thinking, leading to the role of leading the show shows the possibility. This is artificial intelligence (AI), which is not merely a convenience for the emergence of science and technology, but rather plays a role in human emotions, focusing on getting closer to real human beings and making them appear in movies. "Electric Dreams," which focuses on these areas, was the first film to start with the imagination that AI can understand human emotions and act on their own. Computer in the movie plays an important role in creating the narrative as a subject who has got AI in leading the genre of love story. AI in the film "Her", which appears in the form of a more advanced OS with such a material, plays a role of leading the play as an operating system and a heroine who learns deeply and learns and judges like a person who is not a machine. The film art that started from the science created by human beings emerges in various forms in the film by the science and technology that goes beyond the tool expressing the cinematic imagination and competes with the human being and gives a strong message about the future of human existence. However, the science and technology that came into the narrative of the movie is human-programmed artificial intelligence, and it is human being that it is placed in a place where it can evolve by itself. Therefore, artificial intelligence, I think that I can soon see a movie in which artificial intelligence that will comfort human beings and make a deep discourse with human beings will appear soon.

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