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- 저자 : Im Shik Lee (검색결과 62 건)
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Lee Bonggi,An Hye Jin,Kim Dae Hyun,Lee Min-Kyeong,Jeong Hyeon Hak,Chung Ki Wung,고영훈,Seo Arnold Y.,Kim Il Yong,Seong Je Kyung,Yu Byung Pal,LEE, JAE-WON,Im Eunok,Lee In-Kyu,Lee Myung-Shik,Yamada Ken-ich 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
The vitamin-C-synthesizing enzyme senescent marker protein 30 (SMP30) is a cold resistance gene in Drosophila, and vitamin C concentration increases in brown adipose tissue post-cold exposure. However, the roles of SMP30 in thermogenesis are unknown. Here, we tested the molecular mechanism of thermogenesis using wild-type (WT) and vitamin C-deficient SMP30-knockout (KO) mice. SMP30-KO mice gained more weight than WT mice without a change in food intake in response to short-term high-fat diet feeding. Indirect calorimetry and cold-challenge experiments indicated that energy expenditure is lower in SMP30-KO mice, which is associated with decreased thermogenesis in adipose tissues. Therefore, SMP30-KO mice do not lose weight during cold exposure, whereas WT mice lose weight markedly. Mechanistically, the levels of serum FGF21 were notably lower in SMP30-KO mice, and vitamin C supplementation in SMP30-KO mice recovered FGF21 expression and thermogenesis, with a marked reduction in body weight during cold exposure. Further experiments revealed that vitamin C activates PPARα to upregulate FGF21. Our findings demonstrate that SMP30-mediated synthesis of vitamin C activates the PPARα/FGF21 axis, contributing to the maintenance of thermogenesis in mice.
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이종임(Jong-Im Lee),이현순(Hyun-Sun Lee),전우진(Woo-Jin Jun),유광원(Kwang-Won Yu),신동훈(Dong-Hoon Shin),홍범식(Bum-Shik Hong),조홍연(Hong-Yun Cho),양한철(Han-Chul Yang) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.4
정향으로부터 알칼리 추출시 열수 추출보다 3~6배의 높은 항응고 활성을 기대할 수 있었으며 최적 추출조건은 1.0 N NaOH, 70℃임을 알 수 있었다. 정향을 alkali 추출, 에탄올침전, cetavlon 처리 및 한외여과를 이용하여 항응고 활성 획분 EC-2B와 EC-2C를 얻었다. 이 두 획분은 열수 추출보다 각각 6.57배 및 8.63배 높은 항응고 활성획분으로 이는 heparin과 비교시 5.0 unit/mg과 EC-2C는 8.8 unit/mg에 해당되는 활성이다. 이 두 획분을 이용하여 수육을 제조하여 관능검사를 실시한 결과 EC-2B 획분은 정향원물과 유사한 기호도를 가지고 있었으나 EC-2C 획분은 약간의 화학취로 기호도가 낮았다. 보존성 향상효과 또한 정향원물과 정향추출물(EC-2B 및 EC-2C)에서 관찰되었으며 병원성 미생물에 대한 항균 효과 측정결과 두 획분 모두 항균성을 확인할 수 있었다. EC-2B의 경우 황색포도구균은 0.016% 이상부터 EC-2C는 대장균과 황색포도상구균에서는 0.004%에서도 생육 저해효과가 나타났다. EC-2C 획분이 항응고 활성과 항균활성이 우수하나 안전성과 기호도에서 EC-2B가 우수하였다. 따라서 정향으로부터 추출한 두 항응고 획분 중 EC-2B 획분이 기능성 식품의 소재로 적합함을 알 수 있었다. The alkali extraction of anticoagulant from a clove revealed 3-to 6-fold more effective than hot-water extraction. The highest anticoagulant activity was found with 0.1 N NaOH at 70℃. The anticoagulant fractions from a clove, EC-2B and EC-2C were separated by alkali extraction, ethanol precipitation, cetavlon treatment, and ultrafiltration. The anticoagulant activities of these two fractions were, respectively, 6.57 and 8.63 times higher than those extracted with hot-water. As of the sensory evaluation, boiled pork added with EC-2B fraction revealed similar sensory acceptability to raw clove material, while EC-2C fraction had low sensory acceptability due to a mild chemical odor. Antibacterial characteristics against pathogenic microorganism of both fractions were confirmed in the control strain. The inhibitory effect of growth by EC-2B was noticed above 0.016% in S. aureus. Also, EC-2C showed the inhibitory effect at 0.004% in both E. coli and S. aureus control strains.
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정향 추출물의 항응고 활성양식 및 In vivo Test
이종임(Jong-Im Lee),이현순(Hyun-Sun Lee),전우진(Woo-Jin Jun),유광원(Kwang-Won Yu),신동훈(Dong-Hoon Shin),홍범식(Bum-Shik Hong),조홍연(Hong-Yun Cho),양한철(Han-Chul Yang) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.3
EC-2B와 EC-2C 획분은 정향(Eugenia caryophyllata)의 알칼리 추출물로부터 에탄올 침전, cetavlon 처리 및 한외여과를 거쳐 분획하였다. EC-2B 획분은 APTT에서 항응고 활성을 가지는 반면, EC-2C 획분은 APTT와 TT모두에서 항응고 활성을 가지고 있으며 EC-2B와 EC-2C에서 모두 혈소판 응집억제능을 관찰할 수 있었다. EC-2B와 EC-2C 획분의 경구투여에서 두 획분 모두 독성이 없었으며 EC-2B 획분은 1,000 mg/kg (mouse, intravenours)에서도 독성이 없었으나, EC-2C 획분은 LD_(50) 322mg/kg 정도의 독성을 가지고 있었다. 두 획분의 in vivo상에서의 항응고 활성을 60% 생존율을 갖는 dose로 표시한 결과 EC-2B는 131 mg/kg인데 비해 EC-2C는 58mg/kg로 활성의 차이가 in vitro에서 보다 크게 나타났다. 이 두획분을 sulfation시킨 후 활성의 변화를 ex vivo를 통해 확인한 결과 두 획분 모두 활성이 증가하였으며 특히 EC-2B 획분의 활성이 급격히 증가하였다. The EC-2B and EC-2C fractions were ioslated from the alkali extract of Eugenia caryophyllata by ethanol precipitation, cetavlon treatment and ultrafiltration. EC-2B fraction only prolonged the clotting time at activated partial thromboplastin time (APTT), but EC-2C fraction prolonged the clotting time at both APTT and thrombin time (TT). Antiplatelet activity was observed in both fractions. The EC-2B and EC-2C were not toxic upon oral administration. The EC-2B fraction showed no toxicity up to 1,000 mg/ kg (mouse, intravenous), while the EC-2C fraction was 322 mg/kg of LD_(50). The in vivo anticoagulant activities of two fractions were compared at the dose adjusted to 60% survival rate in vitro. The in vivo activities were 131 mg/kg for EC-2B and 58 mg/kg for EC-2C, magnifying the difference in activity between two fractions compared to in vitro activities. After sulfating these two fraction, an increase in the anticoagulant activity was observed in both EC-2B and EC-2C; especially, the activity of EC-2B fraction was dramatically increased.
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이종임(Jong-Im Lee),이현순(Hyun-Sun Lee),전우진(Woo-Jin Jun),유광원(Kwang-Won Yu),신동훈(Dong-Hoon Shin),홍범식(Bum-Shik Hong),조홍연(Hong-Yun Cho),양한철(Han-Chul Yang) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.2
허브를 비롯한 식용되는 채소류로부터 항응고 활성을 검색하기 위하여 93종을 대상으로 냉수(Fr. I), 메탄올(Fr.Ⅱ), 열수(Fr. Ⅲ)추출물을 각각 조제하였다. 내인성 경로에 작용하는 항응고 활성은 수용성 획분인 Fr. I과 Fr. Ⅲ를 대상으로 activated partial thromboplastin time(APTT)으로 검색한 결과 양파, 마늘, 정향, 쑥, 울금 등이 높은 활성을 보였으며, 외인성 경로와 공통경로에 작용하는 항응고 활성은 저분자임을 감안하여 Fr. I과 Fr. Ⅱ를 대상으로 각각 prothrombin time(PT) 과 thrombin time(TT)으로 검색한 결과 민트, 이탈리안 시즈닝, 로즈메리, 타라곤, 울금, 백리향, 와사비는 공통경로에 항응고 활성을 나타내었으나 외인성 경로에 단독으로 항응고 활성을 나타내는 시료는 없었다. 1, 2차 검색 결과 가장 높은 항응고 활성을 보인 정향(Eugenia caryophyllate, clove)을 대상으로 산지를 달리하여 항응고 활성을 검색한 결과 홀랜드산 정향의 활성이 가장 높았다. In order to search for blood anticoagulant substance from herbal extracts, we extracted with cold water (Fr. I), methanol (Fr. II) and hot water (Fr. III) from 93 commercially available herbs. The anticoagulant activity of herbal extracts was examined through the intrinsic pathway by activated partial thromboplastin time (APTT), the extrinsic pathway by prothrombin time (PT) and the common pathway by thrombin time (TT). Onion, garlic, clove, fenugreek, mugwort, thyme and sage had on anticoagulant activity with in- trinsic pathway and mint, italian seasoning, rosemary, turmeric, tarragon and wasabie had on anticoagulant activity with common pathway. Nobody had on anticoagulant activity with extrinsic pathway. Among commercial herbs tested, clove cultivated in holland was selected because it showed the most potent anticoagulant activity among the samples investigated.
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Kim, Hyun-Sun,Lee, Young-Hee,Lee, Young-Ran,Im, Sun-A,Lee, Jae-Kwon,Kim, Yeong-Shik,Sim, Joon-Soo,Choi, Hyung-Seok,Lee, Chong-Kil 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.7
Acharan sulfate isolated from the giant African snail, Achatina fulica, has been reported to have antitumor activity in vivo. In an effort to determine the mechanisms of its antitumor activity, we examined the effects of acharan sulfate on professional antigen presenting cells (APCs). Acharan sulfate increased the phagocytic activity, the production of cytokines such as $TNF-{\alpha}$ and $IL-1{\beta}$, and the release of nitric oxide on a macrophage cell line, Raw 264.7 cells. In addition, acharan sulfate induced phenotypic and functional maturation of immature dendritic cells (DCs). Immature DCs cultured with acharan sulfate expressed higher levels of class II MHC molecules and major co-stimulatory molecules such as B7-1, B7-2, and CD40. Functional maturation of immature DCs cultured in the presence of acharan sulfate was confirmed by the increased allostimulatory capacity and IL-12 production. These results suggest that the antitumor activity of acharan sulfate is partly due to the activation of professional antigen presenting cells.
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이봉수,양진모,안병민,정규원,김부성,한준열,선희식,임계순,백남종,정인식 대한소화기내시경학회 1993 Clinical Endoscopy Vol.13 No.1
The Endascopic Injection Sclerotherapy(EIS) has been widely adopted as a primary treatment for acute hemorrhage from esophageal varices. The long term repeated EIS could achieve esophageal variceal obliteration which has laeen regarded as the endpoint of sclerotherapy by many authors. Several studies showed that repeated EIS improved the 1ot18-term survival, and reduced the bleeding episodes. However, in recent prospective randomized trials, the frequency of bleeding was not reduced and even increasd without improvement of survival in patients undergoing prophylactic sclerotherapy. However, after stabilization of acute bleeding soma patients underwent inital sclerotherapy refuse further sclerotherapy mainly due to severe chest pain and several minor complications related to sclerotherapy. Those patients usually undergo sclerotherapy when the eaophaBeal variceral bleeding recur. The aim of this study was to evaluate whether long term repeated sclerotherapy of planned regimen(EP) is superior to episodic sclerosis in regard to the prevention of rebleeding, and improvement in survival. We enrolled 255 patients with an acute variceal bleeding episode within 3 days at the time of admission in this study from Jan 20th 1987 to Aug 20th 1992 in Dae-jeon St. Mary's Hospital. Among 255 patients, 122 patients were excluded from the study because of combined severe illness at the time of initial sclerosis in I6, lost to follows up in 70, inability to classify due to early death in 4, and small varices(below grade 3 by CMC criteria) in 32. Of the total 133 patients, $quot;r5 patients had received endoscopic sclerotherapy by planned regimen(EP) and 58 patients by episodic sclerotherapy on bleeding(EE). Mean follow up was similar in both groups(EP. 23.3±○ months: EE, 25.5±14.2 months). The sclerotherapy of planned reginten reduced rebleeding(EP, 2.01±1.35; EE, 3.90±2.54) and increased overall obliteration rate(EP, 67%; EE, 14%) during the observation period. Cumulative life table analysis revealed no difference in survival between EP and EE. However, in regared to bleeding as a single cause of death, there was a significant difference in survival(P$lt;0.0001). Complication rate was not different between the two groups(EP, 15%; EE, 9%). There was no death related to sclerotherapy in all patients. Esophageal stricture was most frequent complication in two groups, but it was easily treated by TTS ballon dilatation in all cases. At the end of stady there was no difference in hepatic reserve function between the two groups. We conclude that sclerotherapy of planned regaen is more effective to obliterate esophageal varices, to prevent rebleeding than that of episodic method, thus it can reduce the mortality from rebleeding.
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Kodak 649F Plates를 이용한 Dichromated Gelatin 홀로그래픽 감광재 처리 방법 연구
임용석,이영락,최옥식 嶺南大學校 基礎科學硏究所 1995 基礎科學硏究 Vol.15 No.-
Dichromated gelatin plays an important role to fabricate various kinds of holographic optical elements. In this study, we have investigated the processing method and the characteristics of dichromated gelatin recording materials by using Kodak 649F plates. The processing methods for obtaining high diffraction efficiencies and environmental stability which are necessary for the fabrication of the holographic optical elements are presented. Exposure characteristics, spectral and angular sensitivity, and the degradation of the diffraction efficiency versus time are measured. The maximum diffraction efficiency of the obtained holographic grating is about 90%.
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