Activation of Transient Receptor Potential Melastatin Family Member 8 (TRPM8) Receptors Induces Proinflammatory Cytokine Expressions in Bronchial Epithelial Cells

김주희,Young Sook Jang,Hwan Il Kim,박지영,박성훈,Yong Il Hwang,Seung Hun Jang,Ki-Suck Jung,Hae Sim Park,Choon-Sik Park 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.4

Purpose: Cold air is a major environmental factor that exacerbates asthma. Transient receptor potential melastatin family member 8 (TRPM8) is a cold-sensing channel expressed in the airway epithelium. However, its role in airway inflammation remains unknown. We investigated the role of TRPM8 in innate immune responses in bronchial epithelial cells and asthmatic subjects. Methods: The TRPM8 mRNA and protein expression on BEAS2B human bronchial epithelial cells was examined by real-time polymerase chain reaction (PCR), immunofluorescence staining and western blotting. Additionally, interleukin (IL)-4, IL-6, IL-8, IL-13, IL-25 and thymic stromal lymphopoietin (TSLP) levels before and after menthol, dexamethasone and N-(4-tert-butylphenyl)-4-(3-chloropyridin-2-yl) piperazine-1-carboxamide (BCTC) treatments were measured via real-time PCR. TRPM8 protein levels in the supernatants of induced sputum from asthmatic subjects and normal control subjects were measured using enzyme-linked immunosorbent assay, and mRNA levels in sputum cell lysates were measured using real-time PCR. Results: Treatment with up to 2 mM menthol dose-dependently increased TRPM8 mRNA and protein in BEAS2B cells compared to untreated cells (P < 0.001) and concomitantly increased IL-25 and TSLP mRNA (P < 0.05), but not IL-33 mRNA. BCTC (10 μM) significantly abolished menthol-induced up-regulation of TRPM8 mRNA and protein and IL-25 and TSLP mRNA (P < 0.01). TRPM8 protein levels were higher in the supernatants of induced sputum from asthmatic subjects (n = 107) than in those from healthy controls (n = 19) (P < 0.001), and IL-25, TSLP and IL-33 mRNA levels were concomitantly increased (P < 0.001). Additionally, TRPM8 mRNA levels correlated strongly with those of IL-25 and TSLP (P < 0.001), and TRPM8 protein levels were significantly higher in bronchodilator-responsive asthmatic subjects than in nonresponders. Conclusions: TRPM8 may be involved in the airway epithelial cell innate immune response and a molecular target for the treatment of asthma.

DA-9601, a standardized extract of Artemisia asiatica, blocks TNF-alpha-induced IL-8 and CCL20 production by inhibiting p38 kinase and NF-kappaB pathways in human gastric epithelial cells.

Choi, Suck-Chei,Choi, Eun-Ju,Oh, Hyun-Mee,Lee, SungGa,Lee, Jeong-Kun,Lee, Meung-Su,Shin, Yong-Il,Choi, Suck-Jun,Chae, Jeong-Ryong,Lee, Kang-Min,Lee, Won-Jung,Park, Jae-Sik,Shin, Chang-Yell,Oh, Tae-You WJG Press 2006 WORLD JOURNAL OF GASTROENTEROLOGY Vol.12 No.30

<P>To investigate whether, or how, DA-9601, which is a new gastroprotective agent, inhibits TNF-alpha-induced inflammatory signals in gastric epithelial AGS cells.</P>

Inhibitory Effect of Hwalhyul Composition on Inflammatory Responses in Rheumatoid Arthritis

Lim, Chang-Bum,Yoon, Il-Ji,Oh, Min-Suck The Physiological Society of Korean Medicine and T 2006 동의생리병리학회지 Vol.20 No.6

This study was performed to examine whether HC is effective in controlling molecular components known to be involved in RA, FLS were used to determine possible regulatory effects of HC on levels of inflammatory cytokines. Major findings are summarized as follows. TNF-${\alpha}$ mRNA expression levels in FLS cells which had been repressed by 10, 100 ${\mu}g/m{\ell}$ of HC treatment. IL-6 mRNA expression levels in FLS cells which had been repressed by 10, 100 ${\mu}g/m{\ell}$ of HC treatment. IL-1${\beta}$ mRNA expression levels in FLS cells which had been repressed by 10, 100 ${\mu}g/m{\ell}$ of HC treatment. The present data suggest that FLS which has been activated by IL-1 and TNF-${\alpha}$ co-treatment decreased production of inflammatory cytokines then, HC treatment by repressed the production of these molecules.

거습복합방(祛濕複合方)이 류마토이드 관절염에 미치는 영향

한주원 ( Joo Won Han ),송영일 ( Young Il Song ),윤일지 ( Il Ji Yoon ),오민석 ( Min Suck Oh ) 한방재활의학과학회 2006 한방재활의학과학회지 Vol.16 No.3

Objectives : This study was carried out to know the inhibitory effect of Kuseupbokhap-bang(Qush.fuhe-f.ng; KC) on rheumatoid arthritis in human fibroblast-like synoviocytes(hFLSs). Methods : For this purpose, experiments were performed to measure the cytotoxicity against hFCs and the production of pro-inflammatory cytokines in hFLSs and the production of NO, ROS. Results : KC had no cytotoxicity against hFCs and reduced the production of pro-inflammatory cytokines TNF-α, IL-6, IL-8, IL-1β in hFLSs, increased the production of TIMP-1. As well as KC reduced the production of MMP-3, NOS-II, ICAM-1, the production of Nitric oxide and ROS, and the proliferation of hFLSs in proportion to the concentration of KC significantly. Conclusions : Comparison of the results for this study showed that KC had immunomodulatory effects of suppressing or enhancing.

신선기보탕(神仙氣步湯)이 Collagen Ⅱ 유발관절염(誘發關節炎)의 발생 및 치료에 미치는 영향

구본길 ( Bon Kil Koo ),신혜란 ( Hye Lan Shin ),진광선 ( Kwang Seon Jin ),윤일지 ( Il Ji Yoon ),오민석 ( Min Suck Oh ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.4

Objectives: Cytokines play key roles in spontaneous autoimmune arthritis in CIA(collagen induced arthritis) mice, a model of rheumatoid arthritis. The object of study is to evaluate the effect of IL-6, TNF-α on development of collagen induced arthritis, on humoral and cellular immunity and on the endogenous production of IL-6, TNF-α in DBA/1J mice. Methods: DBA/1J mice were immunized with 100㎍ bovine type II collagen in 0.1㎖ Freund`s complete adjuvant. There are three groups in this study, Which are wild type(WT), CIA-control and CIA-SSKBT(350㎎/㎏). Levels of anti-collagen antibodies, serum IL-6 and TNF-α were measured at the end of study. At week 6, CIA-control group developed arthritis while CIA-SSKBT group showed far less incidence and severity of arthritis. Result and Conclusions: This study shows that SSKBT inhibits inflammatory effect of IL-6, TNF-α.

자이로 안정화 플랫폼의 정밀제어를 위한 적응 마찰 보상

윤석영,박정일 嶺南大學校 工業技術硏究所 1997 工業技術硏究所論文集 Vol.25 No.1

This paper presents a new adaptive friction compensation technique for stabilized platform of a first order plant with nonlinear friction. Nonlinear friction torque is a major source of tracking and stabilization error in precision pointing servo systems. The proposed compensator is composed of nonlinear feedback controller and reference model of a plant without torque friction. Gains of friction compensator are adaptively adjusted to variation of the friction torque of plant. Simulation result shows that the proposed compensator can significantly reduce the nonlinear factors.

보일러 효율향상을 위한 초음파 발생장치의 개발

박정일,이석규,이호균,박준호 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.1

This paper describes the development of ultrasonic generator to enhance the combustion efficiency of the domestic boiler based on the cavitation property of ultrasonic wave. The following procedure shows the proposed energy-saving-precess of the boiler. (1) vibrate the BLT(Bolted Langevin Transducer) type ultrasonic vibrator. (2) amplify the vibrating energy using resonance principle through the designed hem installed before the injection nozzle. The proposed ultrasonic device is designed to produce smaller fuel particles to be mixed homogeneously with air, which finally reduces the fuel consumption of the boiler.

운동부하 흰쥐에서 포도당 증합체가 심근 당원에 미치는 영향

이석강,조성현,김용운,박덕일,김종연,장용찬 대한스포츠의학회 1994 대한스포츠의학회지 Vol.12 No.1

운동부하전 섭취한 탄수화물이 운동흰쥐의 심근 및 골격근이 당원량에 미치는 영향을 측정하여 심근과 골격근의 대사적 차이점의 일단을 연구한 본 논문의 결과는 다음과 같다. 심근과 골격근 당원량(mg/ g wet tissue, 이하 단위 동일)은 정상군에서 심근 2.62±0.51로서 골격근의 3.37±0.58보다 낮았다(p<0.01). 심근 당원량은 정상군이 2.62±0.51이었다. 운동 30분에서 대조군은 정상군의 63%수준으로 감소하였으며(p<0.01), 탄수화물섭취군은 정상군의 86 및 90%수준으로 감소하였으나 통계적으로 유의한 차이는 없었다. 탄수화물 섭취군의 당원량은 대조군의 142 및 137%정도로 양군 모두 대조군보다 유의하게(p<0.01) 높았다. 운동 60분에는 대조군은 운동30분의 78%(정상군의 49%)수준으로 감소하였으며(p<0.05) 탄수화물섭취군은 포도당중합체 및 포도당섭취군에서 각각 운동 30분에 비하여 72(p<0.01, 정상군의 62%), 81%정도(p<0.05, 정상군의 73%)로 감소하였다. 포도당 섭취군의 당원량은 대조군의 147% 정도로 대조군보다 유의하게(p<0.01) 높았다. 운동 120분에서 대조군은 운동 60분에 비하여 63%(정상군의 33%) 수준으로 감소하였으며 (p<0.01) 포도당섭취군은 운동 60분의 69%(정상군의 50%)수준으로 유의하게 감소하였으나, 포도당 중합체 섭취군은 차이가 없었다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군의 202 및 162% 정도로 양군 모두 대조군보다 유의하게(p<0.01)높았다. 골격근 당원량은 정상군이 3.37±0.58 이었다. 운동 30분에서 대조군은 정상군의 69% 수준으로 감소하였으며(p<0.01) 탄수화물섭취군은 포도당 중합체 및 포도당섭취군에서 각각 정상군의 85 및 88% 수준으로 감소하였으나 통계적으로 유의한 차이는 없었다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군의 125 및 129% 정도로 양군 모두 대조군보다 유의하게(p<0.05) 높았다. 운동 60분에는 대조군은 운동 30분의 77%(정상군의 53%) 수준으로 감소하였으며(p<0.05)탄수화물섭취군은 포도당중합체 및 포도당섭취군에서 각각 30분에 비하여 93(정상군의 80%), 89(정상군의 78%) %정도로 감소하였으나 통계적으로 유의하지는 않았다. 포도당중합체 및 포도당 섭취군의 당원량은 각각 대조군의 152 및 149% 정도로 대조군보다 유의하게 (p<0.001) 많았다. 운동 120분에서 대조군은 운동 60분에 비하여 50%(정상군의 26%) 수준으로 감소하였으며(p<0.001) 포도당 섭취군은 운동 60분의 75%(정상군의 58%)수준으로 유의하게(p<0.05) 감소하였으나 포도당 중합체 섭취군은 대조군의 84%(정상군의 67%)정도로 감소하는 경향을 보였다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군우ㅢ 255 및 221% 정도로 양군 모두 대조군보다 유의하게(p<0.001) 높았다. 이상의 결과로 미루어 보아 심근과 골격근 간의 생리, 생화학적 특성의 차이에도 불구하고 탄수화물의 섭취후 중등도 정도의 달리기 운동시 근 당원의 함량에 미치는 영향은 정도의 차이는 있으나 같은 경향을 나타내었으며, 골격근에서 심근보다 더 많은 근 당원 절감효과를 나타내었다. 또한 운동전 탄수화물의 섭취시 이러한 근 당원 절감 효과로 장시간의 운동시 심근 및 골격근 당원의 고갈 지연으로 운동수행능력의 증가가 기대되며, 특히 포도당 보다는 포도당 중합체의 섭취시 효과가 더 크리라고 사료된다. The purpose of this study was to investigate the effect of glucose and glucose plymer ingestion on the concentration of the cardiac muscle glycogen at 30, 60 and 12 minutes of the treadmill running in rats. Rats were randomly assinged to normal, control, and carbohydrate groups. Carbohydrate group was divided into glucose, and glucose polymer ingested rats. The experiment was carried out after overnight fasting. Rats were administrated either water (control) of carbohydrates of formulated glucose(6%), and glucose polymer(6%) via polyethylene tube to the stomaach before treadmill run. Exercise was loaded by a treadmill run at the speed of I ㎞/hr for 30, 60, and 120 minutes. The results of experiment were as follows: Glycogen content of the cardiac muscle was lower than that of the skeletal muscle (plantaris) in normal rats. Glycogen content of the cardiac muscle was decreased in proportion to the exercise time compared to normal in control and carbobydrates ingested rats. It decreased in control, glucose, and glucose polymer compared to normal rats in decreasing order at 120 minutes treadmill running (% change of glycogen content of control, glucose, and glucose polymer compared to normal rats were 33, 50, and 63% in the cardiac muscle at 120 minutes of treadmill running respectively). Glycogen content of the skeletal muscle was decreased in proportion to the exercise time compared to normal in control and corbohydrates ingested rats. It decreased in control, glucose, and glucose polymer compared to normal rats in ecreasing order 120 minutes treadmill running(% change of glycogen content of control, glucose, and glucose polymer compared to normal were 26, 58, and 67% in the cardiac muscle at 120 minutes of treadmill running respectively). Change of the glycogen content of the cardiac and the skeletal muscle during treadmill running after glucose and glucose polymer ingestion showed that both muscles had tendency to decrease in proportion to exercise time similarly in control and carbohydrates ingested rats. Decreasing rate of glycogen content of cardiac and skeletal muscles during treadmill running compared to normal in carbohydrates ingested rats was lower than that of control. From these results, it is suggested glycogen content of cardiac and skeletal muscles had tendency to decrease in proportion to exercise time similarly in control and carbohydrate ingested rats. Glycogen content of cardiac and skeletal muscles in carbohydrate singested rats less decreased than that of control during treadmill running compared to normal. Glucose polymer of the carbohydrates ingested rats in considered as the most effective for glycogen sparing according to exercise time in both muscles. Therefore, intake of carbohydrate, especially glucose polymer, before exercise may be increase performance of the prolonged moderate exercise.

도시유역에서의 유출해석을 위한 시험유역의 개발

서규우,송일준,구본석,김남길 동의대학교 산업기술개발연구소 2001 産業技術硏究誌 Vol.15 No.-

The purpose of this study is development of experiment catchment for hydrological monitoring at Urban Watershed. The location of test basin is Dong-Eui University, Gaya Dong, Pusan, Karea. For the collecting of basic hydrologic data of experiment catchment, topography inquiry and survey was conducted, and sub-basins data, such as areas of test basin and impervious layer, were arranged. On the basis of that result, a drainage pattern was drawn up and for the collection of hydrological data such as the rainfall data and the water-level data-EMS installed at Dong-Eui Univ. in 1998, and AWS-DEU(Automatic Water-level Station-Dong-Eui Univ) installed at the point of the end discharge.

Sleeve 혈관문합술에 대한 조직학적연구

유재덕,이세일,오석준,심기영 大韓成形外科學會 1983 Archives of Plastic Surgery Vol.10 No.2

During the past decade, microsurgery has become clinical routine and resulted in many technical innovations. Inspite of this, microsurgery still entails certain disadvantages; it is time consuming, technically demanding and sometimes results in distressful failure. The sleeve microanastomosis, proximal vessel end is inserted into distal vessel using only two extraluminal sutures, is easier and less time consuming than conventional end to end anastomosis. However, there is a few reports about the histological study to support the beneficial technique of the anastomosis. An animal study was carried out by doing 25 sleeve anastomoses of carotid arteries of white stratin rats and subsequent histological observations in different time interval in order to clarify the modesty of repair in anastomosed vessels. The following are noted as results: Patency rate of the anastomosis was 84%. No aneurysm was found at any site of the anastomosis. The narrowing of lumen of the inserted proximal vessel, the formation of thrombosis & fibrin clot in foci at proximal vessel end, and the loss of intima at the site of anastomosis were noticed in 3 days following the anastomosis. Still narrowing of the lumen of inserted vessel, the formation of thrombosis & fibrin clot in foci at proximal vessel end were persisted with focal necrosis of the media at the stitch area of dista lvessel in one week following anastomosis. Disappearance of thrombosis & fibrin clot on proximal vessel end complete restoration of intimal continuity with slight atrophy of media on distal vessel were noted in 3 week following anastomosis. Neoitima formation at anastomosis site with moderate atrophy of media on distal vessel were noted in 5 week following anastomosis. Subintimal hyperplasia was not observed except in one case of medial necrosis due to wide adventitial stripping in 8 week following anastomosis.