Interleukin-1B(1L-1B) polymorphisms and gastric mucosal levels of IL-Iβ cytokine in Korean patients with gastric cancer

Chang, Young-Woon,Jang, Jae-Young,Kim, Nam-Hoon,Lee, Jae Won,Lee, Hyo Jung,Jung, Woon Won,Dong, Seok-Ho,Kim, Hyo-Jong,Kim, Byung-Ho,Lee, Joung-Il,Rin Chang KYUNG HEE UNIVERSITY MEDICAL CENTER 2006 고황의학상 수상논문집 Vol.21-22 No.-

Interleukin-1B and IL-1 receptor antagonist gene polymorphisms are associated with an increased risk of gastric cancer (GC) in Caucasian populations. However, recent studies could not find any association between IL-1B-511T polymorphism and the risk of GC in Asians. We tested for an association between IL-1 loci polymorphisms with increased gastric mucosal levels of IL-1β and an increased risk of developing GC in a Korean population. Polymorphisms of IL-1A-889, IL-1B-31, IL-1B-511 and IL-1RN were genotyped in 434 controls and 234 patients with GC. Mucosal IL-1β cytokine was measured using an ELISA. The frequencies of IL-1A, IL-1B-511, IL-1B-31 and IL-1RN were not statistically different between controls and all patients with GC. After subclassification of GC, only patients with intestinal-type GC showed a higher frequency of IL-1B-31T homozygotes (OR = 2.2; 95% CI = 1.1-4.3) compared with controls. Risk was also significantly increased in these patients for IL-1B-31T homozygotes compared with patients with diffuse-type GC (OR = 3.4; 95% CI = 1.5-7.7). As in Caucasian populations, linkage disequilibrium between IL-1B-31 and IL-1B-511 was nearly complete, but the pattern of haplotype related to the risk of GC (IL-1B-31T/IL-1B-511C) was opposite (IL-1B-511T/IL-1B-31C). Mucosal IL-1β levels in H. pylori-infected GC patients were higher in patients homozygous for IL-1B-31T compared with IL-1B-31C/T and IL-1B-31C/C. Thus, the combined effects of H. pylori infection and IL-1B-31T/IL-1B-511C polymorphisms with enhanced mucosal IL-1β production contributed to the development of intestinal-type GC in this Korean population.

PLZF⁺ Innate T Cells Support the TGF-β-Dependent Generation of Activated/Memory-Like Regulatory T Cells

Kang, Byung Hyun,Park, Hyo Jin,Park, Hi Jung,Lee, Jae-Il,Park, Seong Hoe,Jung, Kyeong Cheon Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.6

PLZF-expressing invariant natural killer T cells and CD4 T cells are unique subsets of innate T cells. Both are selected via thymocyte-thymocyte interaction, and they contribute to the generation of activated/memory-like CD4 and CD8 T cells in the thymus via the production of IL-4. Here, we investigated whether $PLZF^+$ innate T cells also affect the development and function of $Foxp3^+$ regulatory CD4 T cells. Flow cytometry analysis of the thymus and spleen from both CIITA transgenic C57BL/6 and wild-type BALB/c mice, which have abundant $PLZF^+$ CD4 T cells and invariant natural killer T cells, respectively, revealed that $Foxp3^+$ T cells in these mice exhibited a $CD103^+$ activated/memorylike phenotype. The frequency of $CD103^+$ regulatory T cells was considerably decreased in $PLZF^+$ cell-deficient $CIITA^{Tg}Plzf^{lu/lu}$ and $BALB/c.CD1d^{-/-}$ mice as well as in an IL-4-deficient background, such as in $CIITA^{Tg}IL-4^{-/-}$ and $BALB/c.IL-4^{-/-}$ mice, indicating that the acquisition of an activated/ memory-like phenotype was dependent on $PLZF^+$ innate T cells and IL-4. Using fetal thymic organ culture, we further demonstrated that IL-4 in concert with TGF-${\beta}$ enhanced the acquisition of the activated/memory-like phenotype of regulatory T cells. In functional aspects, the activated/ memory-like phenotype of Treg cells was directly related to their suppressive function; regulatory T cells of $CIITA^{Tg}PIV^{-/-}$ mice more efficiently suppressed ovalbumin-induced allergic airway inflammation compared with their counterparts from wild-type mice. All of these findings suggest that $PLZF^+$ innate T cells also augmented the generation of activated/memory-like regulation via IL-4 production.

PLZF+ Innate T Cells Support the TGF-beta-Dependent Generation of Activated/Memory-Like Regulatory T Cells

Kyeong-Cheon Jung,Byung Hyun Kang,Hyo Jin Park,Hi Jung Park,Jae-il Lee,Seong Hoe Park 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.6

PLZF-expressing invariant natural killer T cells and CD4 T cells are unique subsets of innate T cells. Both are selected via thymocyte-thymocyte interaction, and they contribute to the generation of activated/memory-like CD4 and CD8 T cells in the thymus via the production of IL-4. Here, we investigated whether PLZF+ innate T cells also affect the development and function of Foxp3+ regulatory CD4 T cells. Flow cytometry analysis of the thymus and spleen from both CIITA transgenic C57BL/6 and wild-type BALB/c mice, which have abundant PLZF+ CD4 T cells and invariant natural killer T cells, respectively, revealed that Foxp3+ T cells in these mice exhibited a CD103+ activated/memory-like phenotype. The frequency of CD103+ regulatory T cells was considerably decreased in PLZF+ cell-deficient CII-TATgPlzflu/lu and BALB/c.CD1d−/− mice as well as in an IL-4-deficient background, such as in CIITATgIL-4−/− and BALB/ c.IL-4−/− mice, indicating that the acquisition of an activated/memory-like phenotype was dependent on PLZF+ innate T cells and IL-4. Using fetal thymic organ culture, we further demonstrated that IL-4 in concert with TGF- enhanced the acquisition of the activated/memory-like phenotype of regulatory T cells. In functional aspects, the activated/memory-like phenotype of Treg cells was directly related to their suppressive function; regulatory T cells of CIITATgPIV-/- mice more efficiently suppressed ovalbumin-induced allergic airway inflammation compared with their counterparts from wild-type mice. All of these findings suggest that PLZF+ innate T cells also augmented the generation of activated/memory-like regulation via IL-4 production.

Anti-inflammatory Activity of an Ethanol Extract of Caesalpinia sappan L. in LPS-induced RAW 264.7 Cells

Il Yun Jeong,Chang Hyun Jin,Yong Dae Park,Hyo Jung Lee,Dae Seong Choi,Myung Woo Byun,Yeung Ji Kim 한국식품영양과학회 2008 Preventive Nutrition and Food Science Vol.13 No.4

The anti-inflammatory activities of an ethanol extract of Caesalpinia sappan L. (CS) were investigated in LPS-induced RAW 264.7 cells. Result indicated that CS inhibited the LPS-induced NO production in a dose-dependent manner with an IC?? of 10.9 ㎍/mL. In addition, CS attenuated the iNOS mRNA and protein expression by inhibiting NF-κB activation. CS also suppressed the productions of IL-6 and MCP-1 in a dose-dependent manner, with IC?? values of 15.9 ㎍/mL and 5.47 ㎍/mL, respectively. In addition to the anti-inflammatory activities, CS decreased intracellular ROS formation in the same cells. In conclusion, CS inhibited the production of NO, IL-6 and MCP-1 via a suppression of the NF-κB activation and intracellular ROS generation.

Anti-inflammatory Activity of an Ethanol Extract of Caesalpinia sappan L. in LPS-induced RAW 264.7 Cells

Jeong, Il-Yun,Jin, Chang-Hyun,Park, Yong-Dae,Lee, Hyo-Jung,Choi, Dae-Seong,Byun, Myung-Woo,Kim, Yeung-Ji The Korean Society of Food Science and Nutrition 2008 Preventive Nutrition and Food Science Vol.13 No.4

The anti-inflammatory activities of an ethanol extract of Caesalpinia sappan L. (CS) were investigated in LPS-induced RAW 264.7 cells. Result indicated that CS inhibited the LPS-induced NO production in a dose-dependent manner with an $IC_{50}$ of $10.9\;{\mu}g/mL$. In addition, CS attenuated the iNOS mRNA and protein expression by inhibiting NF-${\kappa}B$ activation. CS also suppressed the productions of IL-6 and MCP-1 in a dose-dependent manner, with $IC_{50}$ values of $15.9\;{\mu}g/mL$ and $5.47\;{\mu}g/mL$, respectively. In addition to the anti-inflammatory activities, CS decreased intracellular ROS formation in the same cells. In conclusion, CS inhibited the production of NO, IL-6 and MCP-1 via a suppression of the NF-${\kappa}B$ activation and intracellular ROS generation.

Anti-inflammatory Activity of Stevia rebaudiana in LPS-induced RAW 264.7 Cells

Il Yun Jeong,Hyo Jung Lee,Chang Hyun Jin,Yong Dae Park,Dae Seong Choi,Min Ah Kang 한국식품영양과학회 2010 Preventive Nutrition and Food Science Vol.15 No.1

Stevia rebaudiana (SR) is an herb used traditionally as a sweetener in Paraguay and Brazil, whose use is spreading to other countries, such as Japan, Korea and China. In addition to its low calorie sweet taste, SR appears to have other beneficial properties, such as hypotensive capabilities and inflammation reduction. To identify the bioactive natural constituents exerting anti-inflammatory activities, we examined the EtOAc fraction of SR. In the inflammatory mediator inhibitory assay from lipopolysaccharide (LPS)-activated macrophages, the EtOAc fraction significantly, and dose dependently, inhibited the enhanced production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) expression. We also found that treatment of cells with the EtOAc fraction significantly inhibited LPS-stimulated nuclear factor-κB (NF-κB) reporter gene expression. Such inhibition of NF-κB was closely associated with the inhibition of interleukin-6 (IL-6) and the monocyte chemoattractant protein-1 (MCP-1). Therefore, we suggest that SR has the potential for development as a functional food for the treatment of immune diseases, such as rheumatoid arthritis and lupus.

Fusobacterium Nucleatum ATCC 25586 Strain의 가토복부에 있어서의 염증반응에 관한 실험적 연구

양일수,박경정,신효근,김오환 全北大學校 齒醫學硏究所 1989 전북치대논문집 Vol.7 No.1

In this experiment, the author examined the inflammatory reaction of the abdominal skin of rabbits after injection of Fusobacterium Nucleatum strain ATCC 25586 preparations. Fusobacterium Nucleatum was cultured in brain heart infusion broth by Okagashi method. Cultures were centrifuged at 10,000 xg for 20 minutes at 4℃, and supernatant and sediment fractions were prepared. 0.2 ml of supernatant fraction was subcutaneously injected into the abdominal skin of rabbits. The sonicated cell suspension caused inflammatory reaction in the rabbit skin, and also the extracts of outer membrane of F. Nucleatum were filled a role in inflamatory reaction of the rabbit skin. As results, the author confirmed the inducing factors of the inflammatory reaction (severe swelling, induration, redness) were in the outer membrane of F. Nucleatum.

재조합 미생물, Escherichia coli MT201를 이용한 L-Threonine 생산성 향상

하정욱,이만효,김기운,황용일 慶南大學校 附設 基礎科學硏究所 2002 硏究論文集 Vol.16 No.-

의료 및 가축사료용으로 폭 넓은 용도를 지니는 _L-threonine의 대량생산을 위하여 _L-aspartate 생합성계 대사 변이주이며 _L-threonine 생산균주로 개량된 E. coli MT201를 이용하였다. _L-Threonine의 생산성 향상을 위한 일환으로 C. glutamicum으로부터 정제·회수된 pyruvate carboxylase 구조유전자 ORF를 E. coli 발현용 plasmid pTrc99A에 도입하여 plasmid pPC를 제작하였다. 변이주 E. coli MT201 균주에 plasmid pPC를 형질전환법으로 도입하여 과량의 _L-threonine을 생산하는 우수한 재조합 균주인 E. coli MT-pPC을 얻었다. Glucose를 단일 탄소원으로 사용하였을 경우 균체의 생육 및 _L-threonine의 생산량이 감소하는 경향을 보였다. 이를 극복하기 위하여 glucose와 citrate(sodium citrate와 ammonium citrate)를 각각 첨가한 결과 sodium citrate에서 우수한 결과를 flask배양을 통하여 관찰하였다. 적절한 sodium citrate의 첨가 비는 탄소원인 glucose와 sodium citrate의 첨가비가 3.5:1.5일 때 _L-threonine 생산량과 생육이 월등히 향상되었다. 이를 바탕으로 발효조를 이용한 유가배양에서 생산량 75.7g/L를 얻었다. 따라서 본 실험을 통하여 _L-threonine의 생산량이 획기적으로 증가하는 재조합 균주를 개발하였고 생산량도 향상시켰다. _L-Threonine, which is used in the medical field for amino acid infusion and in feedstuff for livestock, is an essential amino acid for man and animals. Microorganisms are genetically bred for industrial production of amino acids including _L-threonine. In this study, Escherichia coli MT201, which was obtained with the view of overproducing for _L-threonine, is the metabolic mutant for a biosynthetic pathway of aspartate family. E. coli MT-pPC was obtained for the improvement of _L-threonine production by introducing plasmid pPC, which is an expression vector for pyruvate carboxylase coding gene(pyc) of Corynebacterium glutamicum ATCC13831 at E. coli MT201 strain. When only glucose was used for carbon source in E. coli MT-pPC, cell growth and _L-threonine production was reduced. For solving this problem, we added glucose and citrates in culture media. From the results, sodium citrate was more effective than ammonium citrate and the optimal ratio, which was the most efficient among those ratio employed here, was the 3.5 : 1.5 of glucose to sodium citrate. In our experiment, _L-threonine production, which was 75.7 g/L for 86 hr by E. coli MT-pPC added with 3.5 : 1.5 ratio of the glucose to sodium citrate in fed-batch culture, and these were higher than 1.2 fold of E. coli MT201 and 3.2 fold of E. coli MT-pPC, respectively, in glucose.

비글개에서 신원방우황청심원의 급성 및 아급성독성시험

성하정,권오경,방명주,곽형일,이진영,신대희,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of New wonbangwoohwangchungsimwon (NSCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs (3 males and 3 females) in acute experiments were administered orally with sinlge dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NSCH, 160 ㎎/㎏/day (low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NSCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NSCH in beagle dogs is considered to be safe.

외기요법이 체표온도 변화에 미치는 영향

노일호,김병완,정광조,曺政孝,黃相畯,吳旼錫 대전대학교 한의학연구소 2003 한의학연구소 논문집 Vol.12 No.1

This study is about the relationship between the effect of Radiating Qui Therapy on human body and the resulting subjective qui sensation. Patients were between 30 and 50 years of age. n=21 persons received DITI (n=14 patients were provided, n=9 control group) twice examination with Radiating Qui Therapy. First all patients were examined by DITI. After treatment with Radiating Qui Therapy (n=14) both the treated and the control groups were eaxmined again. Radiating Qui Therapy is provided upright position. After Radiating Qui Therapy 92% of the treated group felt a significant qui sensation and 43.5 % felt a warmth at the abdomen and over the whole body. A data of this study show Radiating Qui Therapy has s stimulating effect on human skin temperature. As disease region and Qui sensation are very closely related to each other results strongly suggest that Radiating Qui Therapy could be used as a treatment for a several disease.