위암에서 Helicobacter pylori cagA, vacA, iceA 유전자와 숙주 Interleukin-1β및 Interleukin-1 수용체 길항제 유전자 다형성

이성훈 ( Seong Hun Lee ),김태오 ( Tae Oh Kim ),이동현 ( Dong Hyun Lee ),박원일 ( Won Il Park ),김광하 ( Gwang Ha Kim ),허정 ( Jeong Heo ),강대환 ( Dae Hwan Kang ),송근암 ( Geun Am Song ),조몽 ( Mong Cho ) 대한내과학회 2006 대한내과학회지 Vol.71 No.1

Background: Both Helicobacter pylori (H. pylori) cagA, vacA, iceA genotype and host IL-1B/IL-1RN polymorphisms play a role in determining the clinical consequences of H. pylori infection. This study aimed to investigate whether there might be any combinations of H. pylori cagA, vacA, iceA genotype and host IL-1B/IL-1RN polymorphisms that are particularly associated with the occurrence of gastric carcinoma in Korean patients. Methods: This study population was comprised of 239 patients with H. pylori infection: 122 with gastric carcinoma and 117 with gastritis only. DNA was isolated from gastric biopsy sample and H. pylori cagA, vacA and iceA genotype were determined by PCR. IL-1B-511 polymorphisms were genotyped by PCR-RFLP and IL-1RN polymorphisms were analyzed with variable number of tandom repeat after PCR. Results: H. pylori cagA, vacA, and iceA genotype were not associated with an increased risk for gastric carcinoma. IL-1B-511*T carriers and IL-1RN*2 carriers did not show increased risk for gastric carcinoma. On combination of bacterial/host genotypes, cagA+/IL-1B-511*T carriers and cagA+/IL-1RN*2 carriers, vacA s1/IL-1B-511*T carriers, vacA s1/IL-1RN*2 carriers, vacA m1/IL-1B-511*T carriers, vacA m1/IL-1RN*2 carriers, iceA1/IL-1B-511*T carriers, iceA1/IL-1RN*2 carriers showed no increased risk of gastric carcinoma. Conclusions: Combined H. pylori cagA, vacA, iceA genotype and host IL-1B/IL-1RN polymorphisms shows no increased risk of gastric carcinoma. Therefore, it seems other endogenous or exogenous factors may play more important role in the development of gastric carcinoma in Korean.(Korean J Med 71:24-37, 2006)

Anti-photoaging effect of fermented agricultural by-products on ultraviolet B-irradiated hairless mouse skin

CHOI, SUN-IL,JUNG, TAE-DONG,CHO, BONG-YEON,CHOI, SEUNG-HYUN,SIM, WAN-SUP,HAN, XIONGGAO,LEE, SANG JONG,KIM, YOUNG-CHEUL,LEE, OK-HWAN UNKNOWN 2019 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.44 No.2

<P> Processed products from agricultural produce generate a large number of agricultural by-products that contain a number of functional substances. These are often discarded owing to the lack of suitable processing methods. The present study investigated the anti-photoaging properties of fermented rice bran (FRB), soybean cake (FSB) and sesame seed cake (FSC) on ultraviolet B (UVB)-irradiated hairless mouse skin. Results indicated that the oral administration of FRB, FSB and FSC effectively inhibited the UVB irradiation-induced expression of matrix metalloproteinase (MMP)-2, MMP-9, MMP-3 and MMP-13. Reverse transcription-quantitative polymerase chain reaction results also demonstrated that FRB, FSB and FSC significantly inhibited the UVB-induced expression of the genes encoding tumor necrosis factor-α, inducible nitric oxide synthase, interleukin (IL)-6 and IL-1β when compared with the UVB-vehicle group (P<0.05). Additionally, collagen degradation and mast cell infiltration were reduced in hairless mouse skin. Furthermore, UVB-induced wrinkle formation was also significantly reduced in mouse skin compared with the UVB-vehicle group (P<0.05). These results reveal that fermented agricultural by-products may serve as potential functional materials with anti-photoaging activities. </P>

천연항균성 물질을 이용한 Vibrio vulnificus의 살균 및 독소생성 억제효과

조성환,서일원,최종덕,전상수,라택균,정수근,강동훈 國立統營水産專門大學 附設 水産科學硏究所 1993 수산과학연구소보고 Vol.4 No.-

생선회용 해산물에 오염되어, 생식한 경우 발생되는 패혈병의 원인균주인 Vibrio vulnificus의 생육 및 독성생성을 억제할 목적으로 천연 항균제인 Grapefruit종자추출물(GPSE)을 이용하여 그 항균효과를 검토하였다. In vitro 시험결과, Vibrio vulnificus에 대한 GFSE의 생육최소저해농도는 50~100 ppm 정도이었으며, Vibrio vulnificus 균체세포틀 100 ppm 농도의 GFSE 용액으로 처리하고 전자현미경 촬영시료로 조제하여 촬영한 후, 미생물형태을 무처리대조구와 비교, 검토한 결과, GFSE 처리로 세포막 기능이 파괴되어 세포내용물이 균체외부로 유출되어 균체의 생육이 억제되고 균체내부가 빈 ghost 형태의 균체수가 증대되어 GFSE의 항균효과를 확인할 수 있었다. 한편, 생선회용 해산물에 GFSE를 근육주사하고 Vibrio vulnificus를 접종한 수조내에서 사육하여 채취한 어육부를 마쇄하여 일정량씩을 표준사료에 첨가해서 통닭용 병아리를 실험동물로 하여 사양실험을 실시한 결과. 체중증가율, 단백질이용효율, 혈청내 효소활성, 혈청의 주요성분함량 등에 있어서, GFSE 처리수조내에서 사육한 해산물을 첨가한 시료시험구의 경우, 무첨가사료처리구와 비교할 때 뚜렷한 차이를 목격할 수 없었으며, GFSE 첨가에 따른 실험동물의 성장저해 또는 독성현상을 유발하지 않음을 알 수 있었다. 아울러, 각 처리구에서 사양한 해산물의 어육부에 오염된 Vibrio vulnificus의 균수를 측정한 결과, 250ppm 이상의 GFSE 처리구에서는 Vibrio vulnificus를 전혀 검출할 수 없었다. To prevent food-poisoning outbreaks by Vibrio vulnificus the antimicrobial efficacy of grapefruit seed extract (GFSE) was examined. Minimal inhibitory concentration (in vitro) for the microorganism was found to be 50~100 ppm. Transmission electron micrographs of Vibrio vulnificus showed the biocidal action of this natural antimicrobial agent would be related to specific respiratory effect coupled with the destruction of permeable function of microbial cell membrane. After Anguilla japonia GFSE-injected to the body was incubated in the seawater contaminated by Vibrio vulnficus the fish meats were taken up, mixed with control diet and used as a diet in the feeding experiment. In this experiment the effect of GFSE treated with fish muscle on body weight protein efficiency ratio, serum enzymes and serum blood component of broiler chicks was investigated. It is proved from this study that there is neither Vibriosis nor toxicity associated with GFSE itself and fish meats treated with it when it is injected to the fish body at a level of 250 ppm or less.

다품종소량 생산관리정보시스템의 개발사례

조규갑,김갑환,문일경,김기영 한국경영과학회 1993 經營 科學 Vol.10 No.2

A production control information system developed for multi-product, small-lot-sized production is presented. The system is composed of interrelated modules for production planning, shop floor control, and inventory / material management. The architecture of the system, functions of each module, and information processing procedures of each function are discussed.

特許權의 相續에 관한 考察

曺日煥 東義大學校 法政硏究所 1999 공공정책연구 Vol.15 No.-

責任能力

曺日煥 東義大學校 法政硏究所 1996 공공정책연구 Vol.12 No.-

경쟁불안이 양궁선수들의 기록에 미치는 영향

조현일,노일환 公州大學校스포츠科學硏究所 1999 스포츠科學硏究所論文集 Vol.- No.13

To accomplish the pupose of this study, l have compared two groups : Superior group of 30 players and less-superior proup of 30 platers to examone the effect of competition anxiety for western style acheries tournament records and training records. The superior group contains the players who have won the prize for national tournament and less-superior group does not but same record in training stage. The differences of tournament records, training records and SCAT score between these two groups have been verified by using t-verification. In addition, pearson's correlationship of these two groups and SCAT score. The results of this study has shown : Frist, SCAT score shows statistical differences between two groups (P〈001) Second. No statistical differences in single and frand training records, but in tournament records(P〈001) Third, the meaninful differences of SCAT score has shown between male and female superior group and less-superior group Fourth, No difference of training records of single and grand but in tournament records of these two groups. Fifth, the SCAT score, has a reliable relation between two groups. Finally, the SCAT score, single and grand training records shows no difference statistically but in tournament records has high correlation between these superior group and less-superior group. According to this study, the competition anxiety has a negative effect for Western-Style acheries in tournament records. The level of this negative effect is greater among less-superior players compared to superior group.

生命權侵害에 대한 損害賠償請求權의 法理

曺日煥 東義大學校地域社會開發硏究所 1985 공공정책연구 Vol.1 No.-

The infringement of right, one of the legal factors for the causation of responsibility which is regarded as an illegal activity, is an infraction of upholding the law benefit according to the private opinion. The illegal activity of infringement of right of life cannot be made unless there is an occurrence in consequence of the victim's death. The provision 750, virtually one of the general stipulations for the illegal activity in the civil law, imposes the indemnity obligation upon a person who inflicts the damages on other person, by intention or lapse. Any infringement of right of life couldn't possibly exist without the termination of the victim's life in accordance with the national stipulation against the infringement of right of life. In order for us to define and assert the infringement of right of life, there accordingly should be ensued a victim's death. Mostly both the constituent of causation of responsibility in the illegal activity and the damages incurred by the infraction of right could be strictly distinguished. The intention or the lapse is involved only in the infringement of right of body. When the victim's death results from both the infringement of right of body and the corresponding causation when the infringement of right of body precipitates a considerable loss, the illegal doer shall be liable to assume the indemnity against the death. Nobody can doubt about a theory of law of illegal activity, and the infringement of that illegal activity is not an infringement of right of life but that of right of body. It should be remembered that the extent of indemnity against the claims which bases on the illegal activity in consequence of the infringement of right of body exerts only the loss incurred by the death.

環境汚染의 民事責任에 關한 硏究(Ⅰ)

曺日煥,金宗植 東義大學校 法政大學 1992 공공정책연구 Vol.8 No.-

危險責任論에 關한 再檢討

曺日煥 東義大學校 法政大學 1993 공공정책연구 Vol.9 No.-