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      • Deoxycytidine kinase and deoxyguanosine kinase of Lactobacillus acidophillus R-26 are colinear products of a single gene

        IKEDA, TAKEHISA,GRIMME, STEPHEN,Ma, NING,IKEDA, SEACHIRO,FIENO, ANGELA,IVES, DAVID H,GUO, SHENYUAN,PARK, INSHIK 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4

        Three of the four deoxynucleoside kinases required for growth of Lactobacillus acidophilus R-26 exist as heterodimeric pairs specific for deoxyadenosine(dAK) and deoxycytidine (dCK) or dAK and deoxyguanosine (dGK). However, only two tandem genes, dak/dgk, are found, and are expressed only as dAK/dGK in transformed Escherichia coli. Sequencing peptides spanning 63% of the native dCK subunit revealed a sequence identical to that deduced from dgk(beginning MTVIVL…), except that dCK lacks residues 2 and 3 (dCK is M-IVL;dGK is ·TVIVL). Also, mass spectrometry indicates that native dCK and dGK subunits are identical in mass adjusted for the first three residues. Furtheromore, the native enzymes have identical isoelectric pH values, indicating an equal number of charged residues. To enable E. coli to express peptide having the native dCK sequence, codons 2 and 3 were deleted from the dgk portion of the tandem genes, resulting in expression of protein having the specificities and regulatory properties of dAK/dCK, including heterotropic stimulation of dAK activity by deoxycytidine or dCTP (not deoxyguanosine or dGTP) and end-product inhibition of the respective activities by dATP and dCTP. Subcloning normal and mutant dgk yielded homodimeric dGK and dCK, respectively. The dCK homodimer strongly resembles human dCK with a low ?? for deoxycytidine, the ability to phosphorylate deoxyadenosine and deoxyguanosine at much higher ?? values, and end-product inhibition by dCTP. Thus two distinct and specific enzymes evidently are derived from a single Lactobacillus gene. The mechanism by which this occurs in vivo has yet to be elucidated.

      • KCI등재
      • Comparison of the implementation status of weight control and physical activities between South Korean and Japanese university students

        ( Tomoko Ikeda ),( Takahiro Ikeda ),( Osamu Aoyagi ),( Tae Hee Choi ),( Nam Ik Han ),( Ye Ju Hong ),( Kwang Soo Koo ),( Youn Shin Nam ) 한국체육학회 2016 국제스포츠과학 학술대회 Vol.2016 No.1

        Purpose: Adolescent Japanese girls have a strong desire to be thin and, therefore, engage in many unhealthy weight-control behaviors such as dieting. In recent years, this drive for thinness has spread to include boys as well as girls. Meanwhile, the desire to be thin is also increasing among adolescents in South Korea. The present study aimed to investigate the correlation between weight-control (WC) behaviors and physical activity (PA) among university students in Japan and South Korea. Method: The subjects comprised 1,833 university students in Japan (male, 454; female, 456) and South Korea (male, 449; female, 474). All subjects were asked whether they engaged in WC behaviors and were queried about the intensity (mild, moderate, and vigorous), number of days per week, and duration of PA. The correlations between sex, WC behaviors, and PA were investigated by country using a chi-square test. Multiple correspondence analysis (MCA) was then applied to investigate overall correlations. Result: The rates of WC behaviors were higher among female students than male students in both countries (58.7% vs. 18.4% in Japan [χ2=151.9***] and 61.1% vs. 42.3% in South Korea [χ2=32.6***]). Regarding PA, male students tended to exercise more days per week than female students in South Korea for all PA intensities. The same tendency was observed in Japan in relation to moderate and vigorous PA, but not for mild PA which was the same for males and females. Two-dimensional (2D) plots based on the results of MCA showed a correlation between not engaging in WC behaviors and performing PA among male students and between engaging in WC behaviors and not performing PA among female students in both Japan and South Korea for mild PA (1D: α=.455, eigenvalue=1.519; 2D: α=.336, eigenvalue=1.337). This tendency became increasingly marked for moderate (1D: α=583, eigenvalue=1.776, 2D: α=.463, eigenvalue=1.531) and vigorous (1D: α=.656, eigenvalue=1.970, 2D: α=.470, eigenvalue=1.544) PA. Conclusion: Female university students in Japan characteristically engaged in WC behaviors without PA, and this tendency became more marked as exercise intensity increased. Female university students in South Korea also engaged in WC behaviors, but they also performed moderate PA; and WC behaviors were associated with PA. Male university students in South Korea engaged in WC behaviors, but these were associated with PA and the number of days per week; and durations of PA were greater than those of female university students in South Korea at all PA intensities. The rates of WC behaviors were low among male university students in Japan; however, the number of days per week and duration of PA were greater than those of male university students in South Korea at all PA intensities. The present findings show that the correlations between WC behaviors and PA observed among university students in South Korea are stronger in Japan.

      • KCI등재

        돈황사본 지론종문헌 Stein 613V 제 21장「經辨五住地煩惱義」에 나타난아비달마 교리에 대해

        IKEDA MASANORI 한국불교학회 2016 韓國佛敎學 Vol.78 No.-

        The aim of my study is to clarify the position of the Dunhuang manuscript Stein 613 verso, one of the most eminent texts of so-called Dilun (地論) School. For that reason, I examined the Abhidharma doctrines found in Chapter 21 of the Stein 613 verso, “Jingbian wuzhudi fannao yi” (經辨五住地煩惱義, the doctrine of the five *sthavāsabhūmis explained in the sūtra). My findings are as follows: 1. Almost all of the following are based on the *Satyasiddhiśāstra (or *Tattvasiddhiśāstra): the Abhidharma doctrines found in the “zhang” (章, the proposition) of the “Jingbian wuzhudi fannao yi,” the definition of avidyā and of *ekadṛṣṭiṣṭhavāsabhūmi, the three *rāgasthavāsabhūmis and the avidyāvāsabhūmi. 2. It is the concept of “the equal catuḥsatya,” found in the definition of *ekadṛṣṭiṣṭhavāsabhūmi, that we should especially examine in these doctrines. This concept is based on the “theory of extinguishing kleśas by contemplating nirodhasatya,” a theory insisted on by the *Satyasiddhiśāstra. We can find the same concept in Baoliang’s (寶亮) (444-509) commentaries on the Mahāyāna Mahāparinirvāṇasūtra (MPS) which is contained in the Da banniepan jing jijie (大般涅槃經集解, Selected Commentaries on the MPS) and in Fascicle 4 of the Da niepan jing yiji (大涅槃經義記卷第四, Commentary on the MPS) copied in Datong (大統) 5 (CE 539) of the West Wei (西魏). 3. Comparing the interpretations of the concept of “the equal catuḥsatya” which are seen in some commentaries on the MPS that were written in the Southern and Northern dynasties, we can determine that the basis of the interpretations shifted from the *Satyasiddhiśāstra to the “pitan” (毘曇, Sarvāstivāda) in the following order: the Da banniepan jing jijie → Fascicle 4 of the Da banniepan jing yiji → Dunhuang manuscript Niepan jing shu (涅槃經疏, the Commentary on the MPS) (BD00260) → Huiyuan’s (慧遠) (523-592) Da banniepan jing yiji (大般涅槃經義記, the Commentary on the MPS). I thus conclude that the “Jingbian wuzhudi fannao yi” is located in the early stage of the current history of Chinese Buddhist thought. 잘 알려진 것처럼 돈황사본 중에 현존하는 지론종 관련 문헌들은 지론종연구를 위한 1차 자료 중 하나이나, 각 문헌의 정확한 성립지나 성립년대, 성립순서 등에 대해서는 아직 충분한 고찰이 이루어지지 않았다. 본고에서는 돈황사본 지론종 문헌 가운데 가장 대표적인 것 중 하나인 Stein 613V의 제21장「經辨五住地煩惱義」에 나타난 아비달마적 교리를 분석함으로써 Stein 613V의사상사적 위치를 밝히고자 한다. 본고의 고찰결과는 다음과 같다. 1. Stein 613V 「經辨五住地煩惱義」의 “章” 부분에서 無明의 정의와 見一處住 地⋅三愛住地⋅無明住地의 정의에 보이는 아비달마 교리는 거의 대부분 『成 實論』에 기초한다. 2. 이 중에서 특히 주목해야 할 것은 見一處住地의 정의 중에 보이는 “四諦 平等”이라는 개념이다. 이것은 『成實論』의 滅諦斷惑說에 기반한 것이며, 『大 般涅槃經集解』의 寶亮(444-509)釋이나 西魏 大統 5년(539) 서사된 『大涅槃經義 記』 권제4에도 동일한 용례를 찾을 수 있다. 3. “四諦平等”의 개념에 대한 남북조 시대 『涅槃經』 주석서의 주석내용을비교하면 『大般涅槃經集解』 → 大統 5년 서사 『大涅槃經義記』 권제4 → 돈황사본 『涅槃經疏』(BD00260) → 慧遠(523-592) 『大般涅槃經義記』의 순으로 『成 實論』에서 「毘曇」으로의 해석이 이행되는 형태를 확인할 수 있다. Stein 613V 「經辨五住地煩惱義」는 이러한 사상사적 흐름에서 초기 단계에 위치한다.

      • KCI등재

        杏雨書屋所藏敦煌文獻『義記』(羽271)の基礎的硏究

        ( Ikeda Masanori ) 동아시아불교문화학회 2013 동아시아불교문화 Vol.0 No.16

        중국 남북조 시대의 남조 불교 융성은 양 무제(梁武帝, 재위 502-549) 시대에 전성기를 이루었는데, 그 전단계로서 주목되는 것이 남제 무제 (南齊武帝, 재위 482-493)의 장자인 문혜태자(文惠太子, 458-492)와 제2자인 경릉문선왕 소자양(竟陵文宣王 蕭子良, 460-494)을 중심으로 하는 남제 영명(永明) 년간(483-494)의 불교 진흥이다. 본고에서 고찰 대상으로 삼는 『의기(義記)』(羽271)는 바로 이 남제 전반기에 있어서의 남조 불교 교리학의 실록 자료로 생각되는 문헌이며, 소자양의 장서 목록등에 기재된 『잡의기(雜義記)』(또는 『의기(義記)』)20권의 잔권(殘卷)일가능성도 있는 지극히 귀중한 신출 자료이다. 본고는 이 『의기』의 기본 성격을 밝히기 위해 기고한 기초연구이며, I. 본문헌에 관한 기본 정보를 정리하며, II. 현존 『의기』에 등장하는 사명(寺名)·승명(僧名)에 입각하여 본 문헌의 성립지·성립연대를 추정하며, III. 또한 현존『의기』의 모두(冒頭) 3장의 후 2자인 1.2 천안 승종법사 해 법신의(天安僧鍾法師解法身義) 와 1.3 상정림 승유법사 해법신의(上定林僧柔法師解法身義) 라는 두 가지 법신의(法身義)를 중심으로 본 문헌의 성립 배경을 살펴 보는 것을 목적으로 한다. 본고의 고찰에서 얻어진 결과는 다음과 같다. 1. 현존 『의기』에 보이는 19개의 사명 거의 모두와 31명 승명의 절반은 『고승전(高僧傳)』및 『명승전(名僧傳)』·『속고승전(續高僧傳)』에서 확실하게 찾아볼 수 있다. 2. 본 문헌의 성립지는 건강(建康) 혹은 그 주변이며, 성립연대의 상한·하한은 각각 483년·489년으로 추정할 수 있다. 3. 본 문헌은 남제의 경릉문선왕 소자양의 장서 목록 등에 기재된 『잡의기』(또는 『의기』)20권의 잔권(殘卷)일 가능성이 있다. 4. 본 문헌은 저명한 승려들이 한 곳에 모여 행한 논의의 기록이라고 생각된다. Prosperity of Buddhism in southern area of China during the Southern and Northern Dynasties reached its golden age under the reign of Liang Wudi 梁武帝 (r. 502-549). For the previous stage of this flourishing, it has been noted by scholars that Buddhism was encouraged in the Yongming 永明 era (483-494) of the Southern Qi 齊 dynasty. During this time, the oldest son of Qi Wudi 齊武帝 (r. 482-493), Wenhui taizi 文惠太子 (458-492), and the second son, Jingling wenxuanwang Xiao Ziliang 竟陵文宣王 蕭子良 (460-494) played central roles. The Dunhuang manuscript Yiji 義記 (hane 羽 271) is a text considered to be a veritable record of the state of Buddhist doctrinal studies of the Southern Dynasties during the first half of the Southern Qi dynasty. This text was recently published, and is extremely valuable to scholars, as it possibly constitutes a surviving orphaned fascicle from the Za yiji 雜義記 (or the Yiji 義記) 20 juan 卷, which was included in Xiao Ziliang`s library list. This article is intended to clarify the basic nature of the Yiji. I focus on the following three goals: (I) to arrange basic information about the text; (II) to estimate the place where the text was composed, and the date of its completion, based on the names of temples and monks appearing in extant part of the Yiji; (III) to investigate the background of the composition of the text, with reference to the two "Fashen yi 法身義," namely "1.2 Tianan Sengzhong fashi Jie fashen yi 天安僧鍾法師解法身義" and "1.3 Shang dinglin Sengrou fashi Jie fashen yi 上定林僧柔法師解法身義," which constitute the latter two of the three chapters that form the beginning of the extant part of Yiji. The findings of the survey of this article are as follows: 1. We can identify almost all of the 19 names of temples and nearly half of the 31 names of monks that appear in the extant part of Yiji, as these temples and monks also appeared in the Gaoseng zhuan 高僧傳, the Mingseng zhuan 名僧傳, and the Xu gaoseng zhuan 續高僧傳. 2. We can estimate that the text was composed in Jiankang 建康, or its outskirts. We can also estimate that the date of its completion was between 483 and 489. 3. The text possibly constitutes a surviving orphaned fascicle from the Za yiji (or the Yiji) 20 juan, which were included in the library list of Jingling wenxuanwang Xiao Ziliang, of the Southern Qi dynasty. 4. The text is considered to be the record of debates held by famous monks, who met together in an assembly.

      • Evaluation of novel sulfonylurea derivatives with a fused heterocyclic moiety as paddy herbicides that control sulfonylurea-resistant weeds

        Ikeda, Hajime,Yamato, Seiji,Kajiwara, Yukari,Nishiyama, Takayuki,Tabuchi, Takanori,Tanaka, Yasushi The Korean Society of Weed Science and The Turfgra 2011 Weed Biology and Management Vol.11 No.3

        Herbicidal activity and acetolactate synthase (ALS) inhibition of sulfonylurea derivatives with a fused heterocyclic moiety bonded to a sulfonyl group were investigated. Some compounds that had an imidazo[1,2-b]pyridazine moiety substituted at the 2-position by chlorine or methyl controlled sulfonylurea-resistant (SU-R) weeds and showed inhibitory activity to ALS prepared from SU-R Schoenoplectus juncoides shoot. There was a correlation between in vitro and whole-plant herbicidal activity of the compounds mentioned above against SU-R Schoenoplectus juncoides. Among them 1-(2-chloro-6-propylimidazo[1,2-b]pyridazin-3-ylsulfonyl)-3-(4,6-dimethoxypyrimidin-2-yl)urea, propyrisulfuron, was selected for further evaluation. Propyrisulfuron effectively controlled paddy weeds at doses of 70 and 140 g a.i. $ha^{-1}$ with good rice selectivity in a field trial.

      • Smart System Identification of Super High-Rise Buildings using Limited Vibration Data during the 2011 Tohoku Earthquake

        Ikeda, A.,Minami, Y.,Fujita, K.,Takewaki, I. Council on Tall Building and Urban Habitat Korea 2014 International journal of high-rise buildings Vol.3 No.4

        A method of smart system identification of super high-rise buildings is proposed in which super high-rise buildings are modeled by a shear-bending system. The method is aimed at finding the story shear and bending stiffnesses of a specific story only from the horizontal floor accelerations. The proposed method uses a set of closed-form expressions for the story shear and bending stiffnesses in terms of the limited floor accelerations and utilizes a reduced shear-bending system with the same number of elements as the observation points. A difficulty of prediction of an unstable specific function in a low frequency range can be overcome by introducing an ARX model and discussing its relation with the Taylor series expansion coefficients of a transfer function. It is demonstrated that the shear-bending system can simulate the vibration records with a reasonable accuracy. It is also shown that the vibration records at two super high-rise buildings during the 2011 Tohoku (Japan) earthquake can be simulated with the proposed method including a technique of inserting degrees of freedom between the vibration recording points. Finally it is discussed further that the time-varying identification of fundamental natural period and stiffnesses can be conducted by setting an appropriate duration of evaluation in the batch least-squares method.

      • SCIESCOPUS

        Probabilistic analysis of buckling loads of structures via extended Koiter law

        Ikeda, Kiyohiro,Ohsaki, Makoto,Sudo, Kentaro,Kitada, Toshiyuki Techno-Press 2009 Structural Engineering and Mechanics, An Int'l Jou Vol.32 No.1

        Initial imperfections, such as initial deflection or remaining stress, cause deterioration of buckling strength of structures. The Koiter imperfection sensitivity law has been extended to describe the mechanism of reduction for structures. The extension is twofold: (1) a number of imperfections are considered, and (2) the second order (minor) imperfections are implemented, in addition to the first order (major) imperfections considered in the Koiter law. Yet, in reality, the variation of external loads is dominant over that of imperfection. In this research, probabilistic evaluation of buckling loads against external loads subjected to probabilistic variation is conducted by extending the concept of imperfection sensitivity. A truss arch subjected to dead and live loads is considered as a numerical example. The mechanism of probabilistic variation of buckling strength of this arch is described by the proposed method, and its reliability is evaluated.

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