Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

논문 : 「권중화개국원종공신록권」에 대한 일검토 : 權仲和開國原從功臣錄券

金炯秀 한국중세사학회 2004 한국중세사연구 Vol.0 No.16

The purpose of this study is to present "Kwon Jung-Hwa's Merit Subject Document for the Foundation of Joseon Dynasty"(權仲和原從功臣錄券) which was included in "Yung-Ga-Se-Go" (永嘉世稿) edited by Lip-Up-Chung-Sa(立嚴精舍) and compare the characteristics of the Merit Subject Document with those of other merit subjects. The Merit Subject Document(功臣錄券) is a secondary record selected for a collection of writings. In addition, the original form of the Document was damaged because considerable parts of the Document were left out in the process of picking out the contents regarding Kwon, Jung-Hwa. Only 53 lines of the Document currently remain with loss of 118 lines and 11 figures are identified on the Document, which indicates the lowest number among other existing merit subject documents. However, merit subjects selected on the Document were officially most highly ranked figures who haven't been found on the other merit subject documents. The figures kept officially high positions at the end of Goryeo Dynasty and didn't actively involve in Lee, Seong-Gye's political revolution except Cho, Hi-Go(趙希古) and Sim, Deog-Boo(沈德符). Most of the figures were highly regarded as respectable senior subjects at that time. The content of reward for the merit subjects of the Document includes thirty kyeols(結) of land and three servants, which is comparable to the reward for figures recorded on "Jeong, Jin's(鄭津) Merit Subject Document". While the principle of reward identified on "Jeong, Jin's Merit Subject Document" took on the standard for Yun, Bang- Kyeong(尹邦慶), the principle of reward on "Kwon, Jung- Hwa's Merit Subject Document" applied the standard for Kim, Lip Kveon(金立堅). As a result, "Kwon, Jung-Hwa's Merit Subject Document" is invaluable for exhibiting an example of merit subject document for the foundation of Joseon Dynasty.

혈우병 환자의 임상 양상에 관한 단일기관 연구

허지혜,김형욱,김영대,이영호,김남수,설인준,김상우 인제대학교 2006 仁濟醫學 Vol.27 No.-

Objective : We analyzed and investigated clinical manifestations, laboratory tests, and inhibitor development of hemophilia that had not been thoroughly studied. Methods : We retrospectively reviewed 96 patients with hemophilia who were registered in the Hanyang University hospital from Jan, 1984 to Dec, 2003, Results : The total patients, whose median age at onset was 46.0 months, in clude 84 cases of type A (87.5%) and 12 cases of type B (12.5%). The most common chief complaint was a traumatic hemorrhage. The family histories of hemophilia were revealed in 41 cases (42.7%). The median activated partial thromboplastin time (aPTT) was 66.7 seconds. The number of severe cases was 39 (40.6%). Inhibitors were present in 13 cases (13.5%) and seven cases (53.8%) were high responders. aPTT and factor levels showed reverse correlation (r=-0.467). aPTT is more prolonged in group without family history than the group with family history (P=0.037). Conclusion : In this study, the diagnosis of hemophilia are made at the lower age and the serological positivity of the HCV is decreased. Activated partial thromboplastin time and factor levels shows reverse correlation. Activated partial thromboplastin time is more prolonged in group without family history compared with group with family history. The frequencies of family history are lower than those of other developed countries, because of concealing the family history of hemophilia. The prevalence of the development of inhibitors increased compared with those of previous studies in Korea, but was lower than those of other countries. Further studies would be necessary to decrease the inhibitor development.

녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

원위 대퇴골의 고립성 골연골종에서 발생한 속발성 연골 육종 1례 보고

정필현,황장수,강석,김용민,오형호,채동주,김종필 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

연골육종은 비교적 서서히 자라며 늦게 전이하는 악성 연골 종양으로 알려져 있다. 골 연골종에서 발생한 속발성 연골육종은 발생빈도는 드물며 타부위로의 전이가 적고 악성도가 낮은 경우가 많아 적합한 수술적 치료로서 좋은 예후를 보이는 것으로 보고되고 있다. 본 동국대학교 정형외과학 교실에서는 우측 대퇴골 원위부 골간단부에 생긴 고립성 골 연골종에서 이차적으로 발생한 연골육종을 치험한 바 문헌고찰과 함께 보고하는 바이다. A case of Secondary Chondrosarcoma Arising from Solitary Osteochondroma of the Distal Femur Department of Orthopaedic Surgery, College of Medicine, DongGuk University Phil Hyun Chung M.D., Jung Su Hwang M.D., Suk Kang M.D., Yang Mln Kim M.D., Hyung Ho Oh M.D., Dong Ju Chae M.D., Jong Pil Kim M.D. Chondrpsarcoma is a malignant cartilage-forming tumor that grows slowly and metastasis of this tumor occurs in late stage. Secondary chondrosarcomas arising from a solitary osteocartilaginous exostosis are rare and those have a better prognosis than other chondrosarcomas, and they rarely metastasize. We present our experience with a case of secondary chondrpsarcoma arising in solitary osteochondroma of distal metaphysis of right femur with clinical details.

금속 촉매를 이용한 poly-Si의 결정화에 관한 연구

金善浩,李秀京,金泰延,康娜榮,金亨駿 弘益大學校 科學技術硏究所 2005 科學技術硏究論文集 Vol.16 No.-

Nickel induced crystallization of amorphous silicon (a-Si) has been studied by selective deposition of Ni on a-Si thin films. The kinetics of crystallization for MIC was found to be greatly enhanced than that of SPC. Ni-induced crystallization is a promising technique for low-temperature fabrication of poly-Si thin-film transistors needed for large area applications. Lateral growth of polycrystalline silicon mediated by the formation of nickel silicide, has been successfully employed for the fabrication of high-performance TFTs. A-Si thin films on which Ni films were deposited with various thickness were crystallized as a function of annealing temperatures. Throughout the thesis work, wise choice of both the thickness of Ni and the annealing temperature was found to produce poly-Si films with high quality, thus potentially resulting in the production of TFTs with high performance.

제 5급 와동에서의 단일용기 상아질 접착제와 자가 산부식 접착제의 상아질에 대한 침투도 평가

김형수,박성호 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.3

Objective : The purpose of this study was to evaluate the resin infiltration into dentin of one-bottle adhesive systems and self-etching primer bonded to Class V cavities using confocal laser scanning microscope(CLSM). Material and Methods : Forty Class V cavities were prepared from freshly extracted caries-free human teeth. These teeth were divided into two groups based on the presence of cervical abrasion: Group Ⅰ, cervical abrasion ; Group Ⅱ, wedge-shaped cavity preparation. Resin-dentin interfaces were produced with two one-bottle dentin bonding systems-ONE COAT BOND(OCB; Coltene®) and Syntac®Sprint^™(SS: VIVADENT)-, one self-etching priming system-CLEARFIL^^™ SE BOND(SB; KURARAY)- and one multistep dentin bonding system-Scotchbond^™ Multi-Purpose(SBMP, 3M Dental Products)-as control according to manufactures instructions. Cavities were restored with Spectrum®(Dentsply). Specimens were immersed in saline for 24 hours and sectioned longitudially with a low-speed diamond disc. The resin dentin interfaces were microscopically observed using CLSM. The quality of resin-inflitrated dentin layers were evaluated by five dentists using 0-4 scale. Results : Confocal laser scanning microscopal investigations using primer labeled with rhodamine B showed that the penetration of the primer occurred along the cavity margins. Statistical analysis using one-way ANOVA followed by Duncan's Multiple Range test revealed that the primer penetration of the group 2(wedge-shaped cavity preparation) was more effective than group 1(cervical abrasion) and that of the gingival interfaces was more effective than the occlusal interfaces. In the one-bottle dentin bonding systems, the resin penetration score of OCB was compatible to SBMP, but those of SS and self-etching priming system, SB were lowet than SBMP.

만성요통 환자의 테이핑 적용 시 체간의 굴곡과 신전의 근력과 지구력의 변화 측정

김수형 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.2

In this study, the muscle strength and endurance of the lumbar at flexion and extension were determined using and isokinetic muscle strength meter (Biodex) in patients with chronic lumbargo (10 male and 10 females), and the changes in muscle strength and endurance of the lumbar at flexion and extension after application of Y-shaped sacrospinalis muscle taping, typically used for patient with lumbargo, were studied. In addition, the sacrospinalis muscle taping of a different shape (I-shaped taping) was applied bilaterally centering on the spine, and the muscle strength and endurance of the lumbar at flexion and extension were determined and compared with those before taping. In addition, the results after application of Y-shaped taping and I-shaped taping were also compared. 1. The extensor muscle strength of the lumbar at loading of 60° /sec before application of kinesio Taping and after application of Y-shaped taping showed the level of significance (p<.05). The flexor muscle strength of the lumbar at loading of 60° / sec before application of kinesio taping and after application of Y-shaped taping did not show the level of significance. 2. The flexor muscle endurance of the lumbar at loading of 90° / sec before application of kinesio Taping and application of Y-shaped taping did now show the level of significance. The extensor muscle endurance of the lumbar at loading of 90° / sec before application of kinesio Taping and after application of Y-shaped taping did not show the level of significance. 3 The extensor muscle strength of the lumbar at loading of 60° /sec before application of kinesio Taping and after application of I-shaped taping show the lever of significance (p<.05). The flexor muscle strength of the lumbar at loading of 60° /sec before application of kinesio Taping and after application of I-shaped taping showed the level of significance (p<.05). 4. The flexor muscle endurance of the lumbar at loading of 90° /sec before application of kinesio taping and after application of I-shaped taping showed the level of significance. The extensor muscle strength of the lumbar at loading of 90°/sec before application of kinesio Taping and after application of I-shaped taping showed the level of significance (p<.05). 5. In comparison between after application of Y-shaped taping and after application of I-shaped taping, the flexor muscle strength of the lumbar at loading of 60° / sec did not show the level of significance. In comparison between after application of Y-shaped taping and after application of I-shaped taping, the extensor muscle strength of the lumbar at loading of 60° /sec showed the level of significance (p<.05). 6. In comparison between after application of Y-shaped taping and after application of I-shaped taping, the flexor muscle strength of the lumbar at loading of 90° / sec did not show the level of significance. In comparison between after application of Y-shaped taping and after application of I-shaped taping, the extensor muscle strength of the lumbar at loading of 90° / sec did not showed the level of significance (p<.05).

간접골성 고정원을 이용한 상악 구치부 원심이동 장치 종류에 따른 치아 이동 양상 평가

김수진,전윤식,정상혁,박선형 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.6

본 연구의 목적은 pendulum 장치, 미니임플란트를 동반한 pendulum장치(펜듈럼), 오픈코일 스프링 및 미니임플란트를 동반한 오픈코일 스프링 이용 시 각각의 치아이동 양상을 3차원적으로 분석하는 데 있었다. 상악 좌측 치조골 및 치아 모형을 제작하고, Calorific machine을 이용하여 모형상에서 대구치를 3 mm 원심이동시켰다. 실험은 5회씩반복 실시하였다. 모델을 전산화 단층 촬영한 후 V-Works를 이용하여 3차원 모델을 제작하였다. Rapidform상에서 3차원적으로 이동방향과 이동량을 계측하였고, 각각의 장치에 관한 통계적 유의성을 검정하였다. 교정용 미니임플란트를 간접 골성 고정원으로 이용하여 오픈코일 스프링으로 구치부를 원심으로 이동시켰을 때가 치체이동에 가장 가까운 이동양상을 보였고, 고정원 소실도 적게 나타났다 (p < 0.05). 오픈코일 스프링, 펜듈럼 장치 모두 미니임플란트를 부가적으로 이용했을 때 고정원 소실량이 적었다 (p < 0.05). 미니임플란트를 이용하지 않은 경우에는 두 장치의 고정원 소실량이 비슷하였다. 미니임플란트의 이용 여부와 관계없이 펜듈럼 장치로 상악 구치 원심 이동 시 오픈코일 스프링에 비해 제1대구치가 조절성 경사이동 양상으로 이동되었고 (p < 0.05), 제2대구치는 비조절성 경사이동 양상을 보였으며, 치관의 협측경사이동이 일어났다 (p < 0.05). 이와 같은 결과를 근거로 간접 골성 고정원을 이용한 오픈코일 스프링이 상악 구치의 원심 치체이동에 가장 효과적인 장치였으며, 펜듈럼 장치를 이용한 구치부 원심이동 시에는 추가적인 조절이 필요하다고 할 수 있다. Objective: The purpose of this study was to compare the three dimensional changes of tooth movement using four different types of maxillary molar distalization appliances; pendulum appliance (PD), mini-implant supported pendulum appliance (MPD), stainless steel open coil spring (SP) and mini-implant supported stainless steel open coil spring (MSP). Methods: These experiments were performed using the Calorific machine which can simulate dynamic tooth movement. Computed tomography (CT) images of the experimental model were taken before and after tooth movement in 1 mm thicknesses and reconstructed into a three dimensional model using V-works 4.0. These reconstructed images were superimposed using Rapidform 2004 and the direction and amount of tooth movement were measured. Results: The mean reciprocal anchor loss ratio at the first premolar was 17 - 19% for the PD and SP groups. The appliances using mini-implants (MPD or MSP) resulted in less anchorage loss (7 - 8%). On application of a pendulum appliance or MPD, distalization was obtained by tipping rather than by bodily movement. Furthermore, the maxillary second molar tipped distally and bucally. But on application of MSP, distalization was achieved almost by bodily movement. Conclusions: Regarding tooth movement patterns during molar distalization, stainless steel open coil spring with indirect skeletal anchorage was relatively superior to other methods.

광주광역시 근대건축물의 건축적 특성에 관한 조사연구 : 현존 근대건축물을 중심으로 Focused on the remained modern architecture

김진안,탁형수,천득염 대한건축학회 2003 대한건축학회논문집 Vol.19 No.11

The existing studies about buildings during modern period, from the Japanese annexation of Korea to the Liberation, are limited around Seoul and major port cities like In-chon and Pu-san. It has to be made sure that architectural development of circumstance at a small or middle-scale city should be simultaneously examined. The purpose of this study is that the materials, which could historically prosepect architectural activities of the times, should be sorted out by examining and discovering modern buildings before they are destroyed, and the opportunity to newie regards modern buildings as a cultural heritage should be constituted.