흰쥐 해마박편에서 콜린성 수용체와 glutamate 유리와의 상호관계에 관한 연구

신동인,김형룡,고성희,김관식,정동균 대한구강생물학회 1990 International Journal of Oral Biology Vol.14 No.2

Present study was performed to clarify the effect of cholinergic agents on the release of glutamic acid employing hippocampal slices. Hippocampal slices(300∼400㎛ thick) were prepared by the method of Kim et al.(1988) and pre-equilibrated in Krebs-bicarbonate medium(KBM, pH 7.4) for 1hr at 37℃. Pre-equilibrated slices were incubated in fresh KBM and then potssium(50mM)-containing KBM for 5 min period. Basal and potassium-induced release of GABA and glutamic acid were determined from recovered medium by HPLC. After 30 min resting period, slices were reincubated in cholinergic agents-containing KBM and cholinergic agent plus potassium-containing medium consecutively for 5 min period each to investigate the effect of cholinergic agent on basal or potassium-induced glutamic acid release from hippocampal slices. The observed results were as follows: 1. The release of glutamic acid induced by the first and second 5 min-exposure of 50mM potassium was 139.7±14.05 nmol and 114.5±10.01 nmol, respectively. When compared with released amounts of glutamic acid during the corresponding spontaneous periods, these were 5.3 and 5.6-fold increase respectively. 2. Acetylcholine(10-1000μM) inhibited potassium-induced glutamic acid release in dose-dependent manner. 3. The inhibition of glutamic acid release caused by acetylcholine(1mM) was antagonized by atropine(50μM) but not by mecamylamine(50μM).

Chlorhexidine의 사용 방법에 따른 치은염 억제 효과의 비교 연구

최종길,신형식 圓光大學校 1991 論文集 Vol.25 No.2

Rinsing with a chlorhexidine gluconate was the most effective chemical method of controlling method dental plaque and gingivitis. The purpose of the study was to determine the effects of different delivery method of chlorhexidine to regulate gingival inflammation. 20 subjects participated in 3 weeks, and recruited for 2 groups. Following a baseline examination, one group was rinsed twice a day and the other was sprayed into the teeth with gingiva twice a day. Examination regarding plaque index(Silness & Loe), gingival index(Loe & Silness) and papillary bleeding index(Saxer & Muhlemann) was performed after 3 weeks. The results were as follow: 1. There was no significant difference between mouthwash and spray in all 3 indicies. 2. In the mouthwash and spray group, all 3 indicies was significantly reduced at week 3.

유치열기와 혼합치열기의 치주상태에 관한 비교 연구

정종원,신형식 圓光大學校 1990 論文集 Vol.24 No.2

The purpose of this study was to determine the prevalence and severity of gingivitis in a periods of deciduous dentition and mixed dentition. 125children with deciduous dentition(5-6 years of age) and 114 with mixed dentition periods(8-9 years of age) took part in the study. Oral hygiene was assessed using the Plaque Index(P.I) and Calculus Index(C.I). Periodontal status was assessed using the Gingival Index(G.I) and periodontal sulcus depth was measured using a William’s periodontal probe. The results obtained were as follows; 1. In the prevalence and severity of gingivitis, the children with mixed dentition were showed higher than these of deciduous dentition and the prevalence and severity of gingivitis was increased with aging. 2. In the mixed dentition, the correlation among P.I., G.I. and S.D. was higher than that of deciduous dentition. 3. There were no differences between male and female for any of the hygiene or periodontal status.

한국인 성인형 치주염의 실험실 표식자 개발에 관한 연구

임석중,신형식,김강주,정종평 대한구강생물학회 1992 International Journal of Oral Biology Vol.16 No.2

The significance of plaque bacteria and its toxin in the etiology of various periodontal diseases has been well established. The purpose of this study is to develop laboratory marker in adult periodontitis. Five patients with adult periodontitis were examined. Bacterial morphotype was observed by phase contrast microscope. Microflora was isolated by selective and nonselective media. Data was analysed between healthy sites and disease sites by paired T-test. Wolinella recta in the disease sites was isolated more frequently than that of healthy sites and the proportion of Actinomyces in the healthy sites were higher than that in the disease sites. These results suggested that Wolinella recta in the disease sites and Actinomyces in the healthy sites might be the marker of adult periodontitis in Koreans. Further study is needed to clarify the in vitro virulence of Wolinella recta.

인삼이 사람태아골모세포의 세포주기조절에 미치는 영향

김대겸,신형식 원광대학교 치의학연구소 2004 圓光齒醫學 Vol.13 No.1

Ginseng Radix(GR) has been used widely as oriental medicine and the effects of it have been investigated by many researchers. The purpose of present study was to investigate the effects of GR on the cell cycle progression and its molecular mechanism in human fetal osteoblast. The result s were as follows. Increased cell proliferation was observed in cells exposed to 100ng/ml, 10ng/ml of GR-1 at 12 hours and 24 hours, 1㎍/㎖ of GR-1 at 48 hours, and 100㎍/㎖, 10㎍/㎖ of GR-2 at 12 hours, all treatment groups of GR-2 at 24 hours(p<0.05). S phase and G1 phase was increased in the group of treated with 100ng/ml Of GR-1, with 10㎍/㎖ and 1㎍/㎖ of OR-2, with 100㎍/㎖ and 10㎍/㎖ of GR-3 in the cell cycle analysis. The cell cycle regulation protein levels of Cyclin D1, Cyclin E, CDK 2, CDK 4 and CDK 6 were increasad in the group of treated with 1㎍/㎖ and 100ng/ml of GR-1, with 10㎍/㎖ and 1㎍/㎖ Of GR-2, with 100㎍/㎖ and 10㎍/㎖ of GR-3. On the other hand, p21 was decreased in the treatment group with 1㎍/㎖ and 100 ng/㎖ of GR-1, with 10㎍/㎖ and 1㎍/㎖ Of GR-2, 10㎍/㎖ Of GR-3, and p53 and p16 was decreased in the treatment group with 100ng/mll Of GR-1, 100㎍/㎖ GR-3 and pRb W ~ S decreased in the all treatment group except 1㎍/㎖ of GR-1. These results suggested that GR increases the cell proliferation and the cell cycle progression in human fetal osteoblast, which is linked to increased cell cycle regulation protein levels of Cyclin D1, Cyclin E, CDK 2, CDK 4, CDK 6 and decreased ell cycle regulation protein levels of p21, pRb.

Hyaluronic Acid가 백서 두개골 결손부 재생에 미치는 영향

김재호,신형식 원광대학교 치의학연구소 2004 圓光齒醫學 Vol.13 No.1

The present study was performed to evaluate the effects of hyaluronic acid (HA) in healing process on calvarial defects in rats. Calvarial defects was surgically created with trephine bur at the rat calvarim. Rats were divided with into control group, hyalwdc acid-treated group, bovine derived xenografts (BDX)-treated group, and BDX plus HA-treated group. At 1, 2, 4, and 8 weeks after surgery, rats were sacrified and obtained specimens prepared with modified M.T stain for light microscopic evaluation. There were no differences between the control and the experimental groups in inflammatory cell infitration, angiogenesis and osteoclastic activity. In HA plus BDX-treated group, new bone formation and osteoblastic acvity were increased than control and BDX-treated group. At 8weeks of HA plus BDX-treated group, bone healing was similar to that of BDX-treated group, but fibrous tissue formation and joining host bone were increased than that of BDX-treated group. Therefore, the use of HA with BDX showed to stimulate bone conduction by synergistic effect. The results of this study suggest that the potential method of HA with bone grafts for promoting the bone formation around bony defect or periodontal bone loss.

성견 2급 치근이개부 병변 치료 시 이종골 이식 및 혈소판 농축 혈장의 골재생에 관한 효과

임성빈,신형식 원광대학교 생체재료·매식연구소 1999 원광생체재료·매식 Vol.8 No.1

New techniques for regenerating the destructed periodontal tissue have been studied for many years. Current acceptable methods of promoting periodontal regeneration are basis of removal of diseased soft tissue, root treatment, guided tissue regeneration, graft materials, and biological mediators. Platelet Rich plasma has been reported as a biological mediator which regulates activities of wound healing progress including cell proliferation , migration, and metabolism. The purpose of this study is to evaluate the effects of using the Platelet Rich plasma as a regeneration promoting agent for furcation involvement defect. Five adult beagle were used in this experiment. The dogs were anesthetized with Ketamin HCl (0.1 ml/kg, IV) and Xylazine hydrochloride (Rompun^ⓡ, Bayer, 0.1 ml/kg, IM) and conventional periodontal prophylaxis were performed with ultrasonic scaler and hand instruments. With inrasulcular and crestal incision, mucoperiosteal flap was elevated. Following decortication with 1/2 high speed round bur, degree Ⅱ furcation defect was made on mandibular third(P3), forth(P4) and fifth(P5) premolar, and stopping was inserted, After 4 weeks, stopping was removed, and bone graft was performed. Ca-P was grafted in P3(experimental group I), combination of Ca-P and plasma rich platelet were grafted in P4(experimental group Ⅱ), and P5 was remained at control group. Systemic antibiotics(gentamicin sulfate) and anlgesics(phenyl butazone) were administrated intramuscular for 2 weeks after surgery. Irrigation with 0.1% Chlorhexidine Gluconate around operate sites was performed during the whole experiment period except one day immediate after surgery. Soft diets were fed through the whole experiment period. After 4, 8 weeks, the animals were sacrificed by perfusion technique. Tissue block was excised including the tooth and prepared for light microscope with Gomori's trichrome staining. At 4 weeks after surgery, there were rapid osteogenesis phenomenon on the defected area of the platelet produced by activated osteoblast. Bone formation was almost completed to the formix of furcation by 8 weeks after surgery. In conclusion, Platelet Rich Plasma can promote rapid osteogenesis during healing of periodontal regeneration.

치주처치를 위한 기구들의 반복조작이 인공치관 변연부에 미치는 영향

김재호,신형식 원광대학교 생체재료·매식연구소 1999 원광생체재료·매식 Vol.8 No.1

The aim of periodontal therapy is a removal of a bacterial plaque but the instrumentation for plaque control has two nature : removal of a bacterial plaque and increase of surface roughness. Complication of instrumentation is enable to damage to the root surface and artificial crown. Therefore this study was conducted to evaluate the effects of repeated instrumentation on the marginal portion of artificial crown. Fifteen proximal surfaces of ten extracted periodontally diseased maxillary first molars were used. The finish line was placed on the root surface, and then the crown was cased and cemented in usual manner. Three kinds of instruments : had curet, ultrasonic scaler, and ultrasonic curet were used. After instrumentation, final polishing was done with rubber cup and pumice. And surface changes were evaluated by stereomicroscope and scaning probe microscope. Roughness was increased after instrumentation in all groups, and was decreased after polishing except ultrasonic scalar group. Roughness in the ultrasonic scaler group was lower than others, and roughness after polishing in the hand curet group was lower than others. These results indicate that polishing procedure is recommended, because periodontal instruments increase the surface roughness and induce the irreversible damage to the marginal portion.

녹용이 치주인대세포의 세포주기조절에 미치는 영향

유승한,신형식 원광대학교 치의학연구소 2003 圓光齒醫學 Vol.12 No.1

Cervi Parvum Cornu(CPC) is that the young horn of deer family and has been traditionally used as a medicine in Eastern. The purpose of present study was to investigate the effects of CPC on cell cycle progression and its molecular mechanism in human periodontal ligament cells (HPDLC). In cell proliferation assay, 1 ng/ml, 100 ng/ml, 1 ㎍/ml and 10 ㎍/ml of CPC were used, all treatment groups increased the cell growth. Maximal cell proliferation was observed in cells exposed to 100 ng/ml of CPC at 4 day, and 10 ng/ml and 100 ng/ml of CPC at 6 days. S phase was increased and G1 phase was decreased in the group treated with 100 ng/ml of CPC in cell cycle analysis. The protein levels of cyclin D1 were not changed, but the levels of cyclin E, cdk 2, cdk 4 and cdk 6 were increased. The protein levels of p21, pRb were decreased as compared to that of control group, but the levels of p53 was not changed in the cells both treated with CPC and untreated. These results suggested that CPC increases the cell proliferation and cell cycle progression in HPDLC, which is linked to an increased cellular levels of cyclin E, cdk 2, cdk 4 and cdk 6, and decreased the levels of p53, p21.

녹용이 사람 태아 골모세포의 세포주기조절에 미치는 영향

양대승,신형식 원광대학교 치의학연구소 2003 圓光齒醫學 Vol.12 No.1

Recently, many natural medicines, whose advantages are less side effects and possibility of long-term use, have been studied for their capacity, their anti-bacterial and anti-inflammatory effects and regenerative potential of periodontal tissues. Cervi Parvum Cornu(CPC) have been traditionally used as an hale, growth, hematogenous, anti-aging, back pain in Eastern medicine. The purpose of present study was to investigate the effects of CPC extract on cell cycle progression and its molecular mechanism in human fetal osteoblasts. CPC extracts (10μg/ml) increased cell proliferation in the human fetal osteoblasts as compared to non-supplemented control. There was no significant change in the G1 and S phase, but a increase in the G2/M phase in 10 μg/ml and 100 μg/ml of CPC extracts group as compared to non-supplemented control. The protein expression of cyclin E, cdk 2, cyclin D, cdk 4, and cdk 6 was higher than that of control group. The level of p21 was lower than that of control. But that of pRb and p16 was not distinguished from control. These results indicate that the increase of cell proliferation by CPC extracts may be due to the increased expression of cyclin E, cdk 2, cyclin D, cdk 4 and cdk 6, and the decreased expression of p21 in human fetal osteoblasts.