Alginate 배양내에서 Transglutaminase가 연골막세포에 미치는 영향

박정선,공차배,김충현,안현정,정미현,성상철,김희중,조현철,이상훈,이명철 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

제주도산 山草, 海藻 및 버섯類 水溶性 抽出物의 抗癌效果

김수현,오창경,고용구,김성홍,오명철 제주대학교 1994 논문집 Vol.39 No.-

제주지역에서 생산되는 식물 중에서 파리풀(지방명;가신새), 삼지구엽초, 미역, 톳, 표고버섯 및 만년버섯(일명;영지)에서 열수 및 냉수추출물을 얻어 마우스 암모델 세포인 sarcoma-180 세포를 ICR 마우스에 이식하여 고형암 성장저지율과 수명연장율에 대한 항암성을 검색한 결과는 다음과 같다. 산초추출물의 항암효과는 파리풀에서 53.4%, 삼지구엽초에서 58.2%의 고형암 성장저지율을 보였다. 해조류추출물의 항암효과는 톳에서 63.1%, 미역에서 38.2%의 고형암 성장저지율을 보였다. 버섯추출물의 항암효과는 만년버섯에서 62.8%, 표고버섯에서 66.4%의 고형암 성장저지율을 보였다. 산초, 해조, 버섯류 각 추출물에서 복수형암에 대한 효과는 매우 낮았다. 모든 시료는 일정량을 초과 투여한 실험군에서 고형암 성장저지효과가 낮거나 수명이 단축되었다. The hot and cole water extracts were obtained from herbs (Phryma leptostachya and Epimedium koeranum), seaweeds(Hizikia fusiforme and Undaria peterseniana), and mushrooms(Gonoderma luciolum and Lentimus deodes) among which have long been known to have antitumor effects. These extracts were tested on growth inhibition rate(I.R) of solid tumor and prolongation rate(P.R) in animal experiment using ICR mouse with a mouse tumor models sarcoma-180 cell. The results were as follows ; The antitumor effects of herb extracts were 53.4% with Phryma leptostachya and 58.2% with Epimedium koeranum in growth inhibition rate of solid tumor. The antitumor effects of seaweed extracts were 63.1% with Hizikia fusiforme and 38.2% with Undaria peterseniana in growth inhibition rate of solid tumor. The antitumor effects of mushroom extracts were 62.8% with Ganoderma luciolum and 66.4% with Lentinus edodes in growth inhibition rate of solid tumor. The effects on ascite tumor of extracts from herbs, seaseeds and mushrooms were so low that no anititumor effect could be recognized. All the samples showed low growth inhibition rate of solid tumor or short life in over dose groups.

果采類 殘留性 農藥의 突然變異 誘發性

오명철,오창경,김성홍,고용구,김수현 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

Salmonella typhimurium TA 균주를 이용하여 과채류 재배에 많이 사용되는 12종의 잔류성 농약에 대한 돌연변이 유발성을 검정하였다. 본 연구에 사용된 12종의 농약 중 Tedion, Monopho, Danoton, Ometon 및 Captan에 대한 돌연변이 유발성은 강하였고, Thalonil, Prosing, EPN, Phentoate, Dicopol 및 Captan에 대한 돌연변이 유발성은 중간 정도였으나, Dicopol은 돌연변이 유발성이 없었다. TA98에서, Monopho와 Danoton은 강한 돌연변이 유발성을 나타내었고, Thalonil, Prosing, EPN, Phentoate, Parathion 및 Sappiran은 중간정도의 돌연변이 유발성, Danoton은 약한 돌연변이 유발성을 나타낸 반면, Dicopol과 Sappiran은 돌연변이 유발성을 나타내지 않았다. 한편, TA100에서는 Tedion, Danoton, Ometon 및 Captan이 강한 돌연변이 유발성을, Thalonil과 Monopho는 중간 정도의 돌연변이 유발성, Prosing, Phentoate, Parathion 및 Sappiran은 약한 돌연변이 유발성을 보인 반면, EPN과 Dicopol은 돌연변이 유발성이 없었다. 따라서, EPN은 구조이동성 돌연변이를, Sappiran은 염기치환성 돌연변이를, 그리고 이들과 Dicopol을 제외한 나머지 9종의 농약은 구조이동성 및 염기치환성 돌연변이를 일으키는 돌연변이원임을 확인하였다. Twelve residual pesticides which applied on the fruits and vegetables cultivation were tested for mutagenic activity using Salmonella typhimurium TA strain. Mutagenic activities against Tedion, Monopho, Danoton, Ometon and Captan of twelve pesticides tested were strong, Thalonil, Prosing, EPN, Phentoate, Dicopol and Captan were medium, and Dicopol were no. Tedion, Monopho and Danoton showed strong mutagenic activities on TA98, Thalonil, Prosing, EPN, Phentoate, Parathion and Sappiran showed medium, and Danoton showed weak, while Dicopol and Sappiran showed no. At the other hand, Tedion, Danoton, Ometon and Captan showed strong mutagenic activities on TA100, Thalonil and Monopho showed medium and Prosing, Phentoate, Parathion and Sappiran showed weak, while EPN and Dicopol showed no. Therefore, EPN identified mutagen which induced only frameshift mutation, Sappiran was only base-substitution, and nine except these and Dicopol of twelve pesticides tested were mutagens which induced both frameshift and base-substitution mutation.

동종 골수이식 후 만성 이식편대숙주질환과 동반되어 신증후군으로 발현된 IgA 신병증 1예

정철권,김현수,박준성,정성현,조도연,최진혁,남동기,임호영,김명성,신규태,임현이,김효철 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.2

동종 골수이식 후 발생하는 신기능부전은 원인이 다양하여 감별에 어려움이 있다. 만성 GVHD는 흉선의 기능저하에 따른 면역관용의 실패로 발생되는 자가면역현상에 의해 발생되는 것으로 알려져 있으며 드물게 신장을 침범하여 신증후군을 일으키기도 한다. CaA의 용량을 줄이거나 투약중지 후 발생한 만성 GVHD에 동반된 신증후군의 경우 조기에 신조직검사를 시행하여 감별진단하고 CsA의 용량을 증량하거나 재투약하여 좋은 치료 결과를 보일 것으로 기대된다. 저자들은 문헌고찰을 통해 동종 골수이식 후 발생된 신증후군이 유사한 임상양상을 보이며 발생기전에 만성 GVHD와 자가면역현상이 연관되어 있음을 확인하였으며 동종 골수이식 후 신증후군의 임상양상을 보이는 환자에서 병리조직학적으로 lgA 신병증을 진단하고 이러한 lgA 신병증이 발생기전에 만성 GVHD와 자가 면역현상이 연관되어 있을 가능성을 최초로 확인하였으며 CsA를 이용하여 성공적으로 치료한 경험을 하였기에 보고하는 바이다. Renal insufficiency is occasionally encountered in allogeneic bone marrow transplantation and its cause is difficult to ascertain. Chronic graft-versus-host disease (GVHD) related to thymic dysfunction is immune-mediated and involves autoreactivity of T-lymphocytes derived from donor marrow to recipient's major histocompatibility complex(MHC) minor antigens. The clinical mainifestations of chronic GVHD are similar to those of autoimmune disease but kidney involvement is rare. Few cases of nephrotic syndrome with membranous nephropathy or minimal change nephrotic syndrome have been reported to be associated with chronic GVHD in allogeneic bone marrow transplant recipient especially after cyclosporine A(CsA) withdrawal, and these cases have responded well to CsA. Therefore, it is prudent to differentiate the cause of post-transplant renal insufficiency using renal biopsy and to start CsA in a case of nephrotic syndrome associated with chronic GVHD as early as possible. We report a case who had a massive proteinuria during the post-allogeneic marrow transplantation period. The cause of nephrotic syndrome was diagnosed as IgA nephropathy by renal biopsy. The patient was successfully treated with reintroduction of CsA.

한국 성인에서 색도플러 초음파를 이용한 척추동맥 혈류량의 정상치

홍현숙,차장규,박성진,조준희,박재성,김대호,이혜경,안현철 대한초음파의학회 2003 ULTRASONOGRAPHY Vol.22 No.3

Lutembacher 증후군 환자 증례 보고

김성범,서정주,곽철훈,김상민,이보라,민선경,황은구,김용인,조욱현,최석구 인제대학교 2008 仁濟醫學 Vol.29 No.-

Lutembacher's syndrome is rare combination of mitral stenosis (MS) and atrial septal defect (ASD). The hemodynamic interplay between the MS and ASD leads to wide variation of clinical presentation. Here we describe a 43-year-old female with Lutembacher's syndrome and tricuspid regurgitation with pulmonary hypertension, who underwent direct closure of ASD and tricuspid valvuloplasty successfully. We also reviewed other literatures in an effort to increase awareness of this condition.

개에서 발생한 척수질환에 대한 추적조사(70예)

황현민,이재연,지현철,이영원,최호정,박성준,김명철,정성목 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2006 動物醫科學硏究誌 Vol.14 No.-

This study was performed to evaluate the prevalence of spinal disorders in dogs, as well as their breed, age, gender and body weight distributions. Seventy cases of caine spinal disoreders presented in the period 2001 and 2006 were reviewed. The diagnosis was obtained by survey radiographs, myelogram, CSF analysis, CT and MRI. The most frequent breed was Maltese(15 cases), and followed by Yorkshire Terrier (10 cases), Pekingese (9 cases). Canine spinal disorders occurred from 7 month to 17 year old dog, mean age was 5.3±3.7 year-old. Canine spinal disorders occurred more often in females(59.7%) than males. Mean body weight was 5.87±7.02 kg. Degenerative (39 cases, 43.3%) and anomalous (24 cases, 26.7%) disorders were dominant in 90 specific disease distribution. This results can be a reference data for the further study.

Protein microarray를 이용한 Apin-단백질의 상호작용에 관한 연구

박주철,박선화,김흥중,박종태,윤성호,김지웅,이태연,손호현 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.5

법랑모세포 분화와 성숙과정에서 OD314의 발현

박주철,안성민,김흥중,정문진,박민주,신인철,손호현 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.5

법랑모세포는 법랑질을 형성하고 유지하는 세포로, 법랑질의 유기기질을 분비하고 법랑질 석회화 과정에도 관여한다. 치아 발생과정에서 법랑모세포의 분화는 순차적인 상피-간엽 상호작용에 의하여 조절되나, 분화나 성숙과정의 정확한 기전은 아직까지 잘 알려져 있지 않다. 최근에 상아모세포에서 처음 발견된 OD314가 치아 발생과정에서 상아질을 형성하는 상아모세포 뿐 아니라 법랑모세포에도 발현된다고 하였다. 이에 본 연구에서는 생쥐 하악 전치의 다양한 시기의 법랑모세포를 이용하여, 형태학적 분석과 in-situ hybridization에 의한 OD314 mRNA의 발현 그리고 OD314 항체를 이용한 면역조직화학적 분석을 통하여 OD314 유전자의 법랑 모세포 분화와 성숙과정에서의 역할을 연구하여 다음과 같은 결과를 얻었다. 1. 형태학적으로 법랑모세포는 분화 단계에 따라 분비 전단계 법랑모세포, 분비기 법랑모세포, 성숙기의 평탄끝 법랑모세포와 성숙기의 주름끝 법랑모세포로 구분되었다. 2. OD314 mRNA는 분비기의 법랑모세포에서부터 발현되기 시작하여 법랑모세포가 성숙해갈 수록 그 발현이 증가하였다. 3. OD314 단백질은 분비 전단계의 법랑모세포에서는 발현되지 않고, 분비기의 법랑모세포에서는 세포질에 전체적으로 발현되었다. 성숙기의 평탄끝 법랑모세포와 주름끝 법랑모세포에서는 세포의 근심과 원심끝단에 OD314 단백질이 강하게 발현되었다. 이상의 결과를 종합하여 OD314는 법랑모세포의 분화와 성숙과정에서 세포질 내부에서 특징적인 역할을 하는 것으로 사료된다. Ameloblasts are responsible for the formation and maintenance of enamel which is an epithelially derived protective covering for teeth. Ameloblast differentiation is controlled by sequential epithelial-mesenchymal interactions. However, little is known about the differentiation and maturation mechanisms. OD314 was firstly identifled from odontoblasts by subtraction between odontoblast/pulp cells and osteoblast/dental papilla cells, even though OD314 protein was also expressed in ameloblast during tooth formation. In this study, to better understand the biologcal function of OD314 during amelogenesis, we examined expression of the OD314 mRNA and protein in various stages of ameloblast differentiation using in-situ hybridization and immunohistochemistry. The results were as follows : 1. The ameloblast showed 4 main morphological and functional stages referred to as the presecretory, secretory, smooth-ended, and ruffle-ended. 2. ○D314 mRNA was expressed in secretory ameloblast and increased according to the maturation of the cells. 3. OD314 protein was not expressed in presecretory ameloblast but expressed in secretory ameloblast and maturative ameloblast. OD314 protein was distributed in entire cytoplasm of secretory ameloblast. However, OD314 was localized at the proxiamal and distal portion of the cytoplasm of smooth- ended and ruffle-ended ameloblast. These results suggest that ○D314 may play important roles in the ameloblast differentiation and maturation.

개에 있어서 耳根部의 Lidocaine 水針에 의한 全身 無痛覺의 誘發

권현주,유건주,이상은,이정연,송근호,김명철,정성목,박성준,김덕환 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Twelve clinically healthy mongrel dogs were used to clarify the possibility of general analgesia induction by lidocaine aquapuncture to auricular root area in the present study. The experimental animals were devided into control (6 heads) and experimental groups (6 heads), respectively. Physiological salines (3 ml/kg) were injected into both auricular root areas in control group. Lidocaine hydrochlorides (3 ml/kg) were injected into both auricular root areas in experimental group. Superficial pain, the changes of vital signs and blood leukocytes, and PCV were examined on pre, 5 min., 15 min., 30 min, and 60 min. after injection in control and experimental groups, respectively. Analgesia was not detected at all in control group, however, all experimental animals were recumbent and analgesia was generally detected in the auricles, noses, dorsal parts, ventral areas, limbs and tails from 5 to 30 minutes after injection with lidocaine hydrochloride into auricular root areas in experimental group. As for the change of vital signs, body temperature showed decreasing tendency from 5 minutes after lidocaine injection in experimental group. Heart rates and respiration rates were increased on 5 minutes after lidocaine injection and then they showed decreasing tendency in experimental group. No significant difference were found among groups. As for the changes of hematological findings, the numbers of blood leukocytes were increased till 15 minutes after lidocaine injection and then they showed decreasing tendency. PCV values were increased on 5 minutes after lidocaine injection, and then they showed decreasing tendency. However, no significant differences were observed among groups. Considering the above findings, it was thought that general analgesia was possible by aquapuncture with lidocaine hydrochloride into auricular root areas and lidocaine aquapuncture didn’t affect so much to the changes of vital signs and blood.