In Vitro Fertilization and Embryo Cleavage in Canine Oocytes

Hae }ung Kim,Sang Ryeul Lee,Sin Hae Park,Sung Hyun Kim,Bong Soo Kim,Dong Hun Yoo,Sin Hae Park,Mi Jung Shin,Jae Wook Kim,Zae Young Ryoo 한국동물생명공학회(구 한국동물번식학회) 2006 Reproductive & developmental biology Vol.30 No.2

자궁내 태아사망의 임상적 고찰

이권해,이정재,조현철,이석민,이임순,이해혁,김권대,이순곤,남계현 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

Objective: This study was designed to evaluate diagnosis and complication, prognosis during clinical study of cases of intrauterine fetal death. Methods and materials: This is a clinical study of 56 cases of the intrauterine fetal death among 8064 deliveries at Seoul Sunchunhyang university hospital during 5 years from January, 1994 to December, 1998. Results: The incidence was 0.7%. The age distribution of mothers was 18 - 44 years and was highest in the 25 - 29 years range(46.4%). The parity was the most highest in the nulliparous group(51.8%). The most common gestational weeks was 20 - 28 weeks gestational period(46.4%), and most common presentation was cephalic(73.5%). The most common weights of the intrauterine fetal death was from 5,00gm to 1,000g(48.2%). The mode of the most common delivery was the induction of delivery(57.2%). The etiologic factors were: unknown(42.9%), preeclampsia(17.9%), chorioamnionitis(14.3%), congenital anomaly(12.5%), abruptio placenta(7.1%), cord complication(3.6%), placenta previa, death of one for twin. There were 2 cases of maternal complication(DIC, Uterine atony). Conclusion : Intrauterine fetal death should be diagnosised early and treated properly to reduce risk of the maternal complication.

뉴로-퍼지 논리를 이용한 12경락의 양도락진단 시스템의 구현

김병화,한권상,이우철,김성현,최해성,안현식,김도현 대한전자공학회 1997 秋季綜合學術大會 論文集 Vol.20 No.2

머리염색이 인체에 미치는 영향

윤형식,황성호,이현륭,김수호,박연석,권낙현,정호진,김동훈,노현주,홍성호,박병찬,이관,정해관 東國大學校醫學硏究所 2002 東國醫學 Vol.9 No.1

일상생활에서 모발염색은 흔히 접할 수 있는 미용의 한 종류로 특히 젊은층을 중심으로 폭발적으로 유행하고 있다. 염색을 위항 사용하는 약제는 표백제와 발색제 등 각종 화학약품이 사용되고 있으나 이로 인한 건강장해에 대한 연구는 그리 많지 않다. 저자들은 염색이 인체의 모발건강에 미치는 영향을 파악하기 위하여 염색과 관련된 주관적 증상과 모발의 변화에 대한 실험적 연구를 시행하였다. 동국대학교 경주 캠퍼스 재학생 80명을 대상으로 설문조사를 시행하여 염색 유 ·무 및 염색 후에 경험한 증상에 대해 설문 조사하였고, 의과대학 재학생 46명을 대상으로 피부 반응 테스트를 실시하였다. 또한 염색 전후의 모발 탄성도를 측정하였고 모발의 상태를 파악하기 위해 전자 현미경검사를 실시하였다. 설문조사 결과 염색 전에 비하여 염색 후 안구혼탁, 안구건조, 시력저하, 발진 및 접촉성 피부염, 모발손상, 모근손상 등의 증상을 더 많이 경험한다고 호소하였다(p<0.05). 모발손상과 모근손상은 헤어드라이어 사용 빈도에 따라 증가하는 것으로 조사되었다(p<0.05). 피부반응검사에서 가려움증이 가장 많은 증상이었으며 이는 여성보다는 남성에서 높은 것으로 조사되었다. 염색 전후의 모발장력은 염색 전 134.5±10.37g, 염색 128.0±30.69g, 염색 이틀 후 112.5±19.69g으로 나타났다. 염색 전후의 모발의 전자현미경 케라틴 층이 현저히 감소하고 모발이 가늘어지는 차이를 보였다. 염색은 모발손상, 모발 케라틴 손상 및 모근 손상, 발진 및 접촉성 피부반응, 안구혼탁, 안구건조, 시력 저하를 유발한다. 따라서 염색약으로 인한 손상에 대한 주의와 예방이 필요하다고 생각한다. 예방대책으로 염색 전 피부테스트를 통한 적합성 여부를 판단하는 것이 필요하며 가급적 염색을 피하는 것이 좋을 것이다. 염색약에 발암물질이 포함되어있다는 보고도 있어 염색 제조사의 철저한 실험과 염색 물질의 선별이 염색으로 인한 부작용을 최소화하는데 중요한 역할을 할 것이다. Hair coloring has became one of the most popular cosmetic activities to younger generations during last decade. However, there are few studies on the health effect of widespread use of chemical dyes. This study was conducted to study the effects of hair coloring dye on hair and other systems. We conducted a questionnaire survey of 80 persons in Kyongju campus, Dongguk University. We have done open patch skin test on 46 medical students. We also conducted scanning electron microscopy to examine the hair strength and structure before and after hair coloring process. Injury of hair and hair bulb, contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity were the main symptoms complained after hair coloring (p<0.05). Injury of hair and hair bulb were increased by frequency of hair-dryer use(p<0.05). In open patch test, pruritus was complanined by more than half of the subjects. Mean strength of hairs before and after hair coloring was as follows; 134.5 (SD 10.37)g before hair coloring, 128.0 (SD 30.69)g immediately after hair coloring, and 112.5 (SD 19.69)g after two days. The scanning electron microscopic findings of hair surface before and after hair coloring showed decreased keratin layer and thinning of the hair. Hair coloring induces injury to hair, its keratin layer, and hair bulb as well as contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity. Therefore, we think that precaution is needed in use of hair coloring dye. To prevent complications induced by hair coloring dye, it is necessary, especially to those with allergy or skin disorders, to perform skin test before action and avoid hair coloring whenever possible. Longterm health effects of hairdye should be studied and manufacturing companies should try to minimize complications induced by hair coloring dye.

팥 신장기 과습처리에 의한 생육특성 및 단백질 발현

정해룡, 유장환, 윤성현, 권수정, 우선희 충북대학교 농업과학기술연구소 2022 農業科學硏究 Vol.38 No.2

Adzuki bean is sensitive to waterlogging stress. The overall study on the waterlogging stress is limited comparing to the study on the drought and any environmental stress. Therefore, the present study was conducted to investigate the changes in growth characteristics and the expression patterns of proteins at the 5-leaf stage periods of adzuki bean. The domestic cultivar, Arari (Miryang No. 8) was used to test the waterlogging stress. In the waterlogging treatment for 3 days, the plant height showed slightly decrease in the treatment at 3 days of waterlogging, but root fresh weight showed significantly changes at 3 days of the waterlogging treatment. Chlorophyll contents showed also significantly different at 3 days of waterlogging treatment compared to control the plants. The waterlogging stress gradually influenced the growth differences between the control and the treatment respectively. More than 350 pro- tein spots were identified on 2-D gels using an image analysis. Moreover, a total of 28 proteins were analyzed using LTQ-FT-ICR MS. Among these 28 proteins, a total of 18 proteins were up-regulated, and 10 proteins were down-regulated under waterlogging treatment. According to biological process, the most of the proteins were found to be involved in carbohydrate metabolism. Regarding the subcellular local- ization, most of the proteins were localized into chloroplasts in 5-leaf stage.

방사능 동위원소(P-32)를 이용한 감귤나무의 인산 영양 진단법의 개발

현해남,송성준,한해룡,유장걸,김형옥 제주대학교 방사능이용연구소 1989 연구보고 Vol.4 No.-

산림의 인산 영양진단에 적용했던 P-32 bioassay법을 영양특성이 다른 감귤나무에 직접 이용이 곤란하므로 궁천조생과 흥진조생 감귤나무를 공시 재료로 하여 사경과 토경재배 그리고 포장실험을 통해서 P-32 bioassay법과 관련된 기초 연구를 수행하였다. 1. 사경과 토경으로 재배된 2년생 감귤나무(궁천, 흥진조생)로 부터 뿌리를 채취하여 P-32 bioassay를 실시한 결과 인산 공급량과 인흡수능간에 고도의 지수적인 역상관을 보였다. 2. 한편, 야외포장실험의 경우 5월에는 인흡수능과 시비량간에 일정한 경향을 보이지 않았으나 7월과 9월에는 시비량이 증가함에 따라 인 흡수능은 감소하는 경향을 보였다. 이는 감귤나무가 인산에 대한 시비반응이 느리다는 사실을 암시한다. 또한, 인흡수능을 계절별로 봤을 때, 7월과 9월보다는 5월에 가장 컸으며 수령이나 표고보다는 토양의 비옥도에 의해 영향을 받고 있었다. 3. 인흡수능-유효인산-엽중인농도간의 상관을 비교할때 인흡수능-유효인산과 인흡수능-엽중인농도간의 상관이 유효인산-엽중인농도보다 컸음을 감안할때 P-32 bioassay법이 감귤의 인산영양진단에 효율적인 방법이 되리라고 생각한다. 4. P-32 bioassay를 실시하기 위해 채취된 뿌리는 저온보다는 실온에 저장하는것이 근활성을 유지하는데 효과적이었다. 5. 감귤나무의 근활성은 수관밑의 0∼5㎝ 표층에서 가장 높았으며 이는 다년간 표층시비에 기인되어 양분흡수에 관여하는 세근이 표층에 분포되었음을 시사하는 것이다. P-32 bioassay technique which were developed for determining forest tree's P-deficiency was introduced in order to provide an effective diagnosis method of phosphorus status in citrus tree. Some modification of the methods should be done because the physiological characteristics of forest trees and citrus trees are quite different, even though the basic principle is same. Thus, the fundamental steps needed to conduct the phosphorus diagnosis were investigated in the sand culture, pot culture and field experiment using two cultivars “Kung-Chun” and “Hung-Jin” of citrus trees. 1) The exponential relationships were obtained both in Kung-Chun and Hung-Jin from the sand culture experiment and the pot experiment. 2) No correlation between fertilizer levels and bioassay P-uptake was found from the field experiment in May, but in July and September there was a tendancy that highter fertilization reduced bioassay P-uptake, even though that was not statistically significant. Seasonal changes bioassay P-uptake were observed from all the fields. Soil fertility was more closely related with bioassay P-uptake than tree ages and plantation altitudes. 3) The degree of correlation of bioassaay P-uptake and soil available phosphorus or P-contents in leaves was higher than that of soil available phosphorus and P-contents in leaves, indicating that P-32 bioassay method is more sensitive than the conventional ones(soil and plant analyses) to make the P-status diagnosis. 4) The kept ar room temperature had higher P-32 absorption activity than those at 4℃ 5) The active fine roots were distributed mostly at the upper layer(0∼5㎝) of soil and around at the distance of canopy edge.

신경회로망에서 뉴런간의 연결도를 이용한 4백 배치 문제 해결 알고리즘

현해진,김덕주,강부식,이기태,임재윤 濟州大學校 工科大學 産業技術硏究所 1993 産業技術硏究報告 Vol.4 No.-

In this paper we have proposed a method for solving a four-coloring problem using adjusted information transmission between adjacent neurons in neural networks. These algorithms are based on McCclloch-Pitts binary neuron model neural networks. If a map shares n regions, a 4×n neural array is used to color a map of n regions where each neuron as a processing element performs it. By programming these algorithm by a C-language in IBM PC 486 DX and adapting it to various examples, we have showed the ability of our system. If one neuron is selected among four neurons, the strength of order three neurons is wakened. So, by transmitting the information of selected neuron, adjacent neurons cannot share the same color.

금자양중(가네코 카즈시게) 기증 박형 호의 복원 : -경질토기를 중심으로-

황현성,이해순,이용희 국립중앙박물관 2001 박물관보존과학 Vol.3 No.-

이번 가네코 카즈시게 선생이 기증한 여러 유물 중 金子124 수호는 약 60여개 정도의 파편을 접합하고, 자체 하중을 견딜 수 있는 접착제를 적절하게 사용하여 결손부분을 복원하였다. 또한 보존처리를 하는 동안에도 항상 가역성을 염두에 두면서 처리하였고, 색맞춤도 미관상 어색함이 없는 범위 내에서 처리하였다. 그 결과, 기형이 둥근 점토질의 박형(珀形) 항아리로 새롭게 탄생하였다. This article examines the conservation process of a donated Gourd-style jar (Object No. Geumja No. 124), which was broken into several fragments (more than 60) in the past. After assessing the condition of the object, each of the fragments were re-attached, and missing areas were gap-filled and re-touched. The authors discuss considerations, which were taken into account during the decision making process. These include the integrity of the object and the section of treatment materials, such as the reversibility of adhesives and filling materials. After its conservation, the authors believe that the object is in a better condition and ready for display in the future.

송이를 이용한 방울토마토 養液裁培時 生育 및 養液과 植物體 主要成分 含量의 推移에 關하여

玄海男,吳大民,張田益 濟州大學校 亞熱帶農業硏究所 1995 亞熱帶農業硏究 Vol.12 No.-

養液栽培에서 제주송이가 다른 培地와 비교하여 방울토마토의 生育에 미치는 영향과 養液成分 變化와 토마토 식물체내의 주요성분을 분석하여 제주송이를 養液栽培用 固形培地로 실용화하기 위하여 시험할 결과는 다음과 같다. 1. 乾葉比, 乾果比, 糖酸比는 송이 區中 1日 1回 1時間 湛液後 완전 排水한 區에서 높았다. 2. 방울토마토 生果重은 上位 花房으로 갈수록 rock wool區와 湛液水耕區는 작아졌으나, 송이區와 日向土區, perlite區등 固形培地耕에서는 무거웠다. 3. 방울토마토의 生育이 旺盛한 시기에 養液成分중 多量元素를 분석한 결과는 송이 培地區에서 燐酸과 칼슘 濃度가 낮았다. 4. 방울토마토의 수량과 당도는 rock wool과 日向土區에서 높은 경향을 보였다. 5. 일반적으로 養液栽培에서는 水分의 蒸發과 植物의 수분 吸收 蒸散作用으로 肥料鹽의 濃度가 높아가는데 송이區도 다른 固形培地耕과 비슷한 결과를 보였다. 6. 방울토마토 植物體의 窒素含量은 生育 전반기에 많았고 후반기에 적은 경향을 보인 반면 칼슘과 마그네슘은 生育 후반기에 그 含量이 많았다. 한편 燐酸과 칼슘은 전 生育其間을 통하여 변화를 보이지 않았다. 7. 濟州 송이 養液栽培用 固形培地로 손쉽게 이용할 수 있는 가벼운 資材로 加工開發 硏究가 있어야 할 것으로 본다. The main purpose of these studies were to clarify difference in Cheju-scoria and other solid media on growth and development of cherry tomatoes and on shift of component of the nutrient solution, seasonal change of content in tomato plant and to use practically Cheju-scoria as an excellent solid culture medium. The results obtained were summarized as follows; 1. Among scoria plots, the rares of dry weight, fruits and their sugar-acid ratio were higher in the plot was drained well with deep flow for one hour once a day. 2. Fresh fruits weights were lighter in rock wood and deep flow technique, but larger in scoria, Hyugashi (artificial gravel, Φ10∼12mm) and perlite in moving to higher flower cluster. 3. The results of analysis on macroelement among solution components showed decrease of concentrations of P and K in the period of growth and development. 4. Yeilds and brix of cherry tomato showed a tendency to increase in rock wool and Hyugashi than anothers. 5. The concentration of fertilizer base was increased in general solution culture. Transpiration and absorption were similar in Scoria plot and other media. 6. Nitrogen content in cherry tomato leaf showed a tendency to decrease at the latter half of the growth and development than the first half, and yet calcium and magnensium content were in the latter half. In the case of phosphorus and potassium showed no change the change in the growing period. 7. More studies of the Cheju-scoria development is required in order to use it as a soild medium for solution culture.

Zn_0.4Cd_0.6Te 단결정의 적외선 발광소자에 관한 기초연구

강현식,장정근,이해익 全北大學校 基礎科學硏究所 1991 基礎科學 Vol.14 No.2

Zn_0.4Cd_0.6Te : Li의 p형 단결정을 Bridgman법으로 성장시키고, 이결정들의 전기적 및 광학적 특성을 조사하였다. 또 이 결정에 Al을 확산시켜 Zn_0.4Cd_0.6Te : Li-Al의 p-n 동성집합 LED을 제작하였고, 이 LED의 특성, 즉 I-V, EL spectrum 및 발광강도의 전류의존성 등을 조사하여 발광천이 관계를 추정하였다. 또 모체결정의 열처리에 따른 발광특성의 변화도 아울러 조사하였고 끝으로 이들의 에너지밴드 모형과 발광천이 관계를 추정하였다. The pure and Li doped Zn_0.4Cd_0.6Te single srystals were grown by Bridgman method respectively, and the their electro-optical properties were investigated by transmission, photoluminescence and Hall effect mesurements. Through carring out the Al vapor-diffusion into the Li doped Zn_0.4Cd_0.6Te crystals in thermal process, the p-n homojunction LEDs, Zn_0.4Cd_0.6Te:Li-Al, were fabricated. The characteristics of LEDs were investigated by measuring the current-voltage, the electroluminescence spectra and emitting light intensity. And also, the characteristics of LEDs prepared with various thermal anealing were compared with each other. Finally, the energy band model were estimated, and the light emitting mechanism was presumed.