Application of Artificial Insemination Technology for Dairy Breeding in Mongolia

Hyun‐Tae Jo,Jong‐In Jin,Seong‐Su Kim,Byung‐Hyun Choi,Tumor Baldan,Jung‐Gyu Lee,Yun‐Shik Kim,Sam‐Churl Kim,Il‐Keun Kong 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

This study was focused on improvement of milk production in Mongolia dairy industry by artificial insemination (AI) technology, of which was supported from ODA project of KOICA in Republic of Korea. The study was started from January 2009 to present and 3rd years in this year. So, all data, especially synchronization and pregnancy of dairy cows (Holstein) will be summarized in final result in this year. For synchronization, total 81 dairy cows selected from 4 private farms that were 38, 30, 8 and 5 in Undarmal milk, Onjin (Enkhbayer), Jargalant, and BRM School, respectively. All the dairy cows were injected intramuscular (IM) of 5 ml PGF2α in the vulva and detected estrus 2 to 3 days after PGF2α injection. Total 78 out of 81 dairy cows (96.3%) were detected estrus by only 1 time injection of PGF2α. The dairy cows that were induced estrus, inseminated with 0.5 ml dairy frozen semen by conventional AI techniques. The pregnancy diagnosis of the AI dairy cows was detected by uterus palpation after 60 days of insemination. Total 75 from 78 inseminated dairy cows (90.1%) were diagnosis pregnant. The estrus induction and pregnant rate were very effective using PGF2α injection and conventional AI techniques in Mongolia dairy cow. The results indicated that AI after estrus induction in Mongolia dairy cows could be applied to dairy breeding technology to improve the breeding efficiency and milk production.

쇄석분진을 이용한 발포 건축재의 제조

김태훈,김기현,김현태 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.1

A rock dust as industrial waste during rock crushing was recycled to make a porous ceramics. Raw materials were mixed as a portion of rock dust(70vo1%), saw dust(30vo1%), bloating agents(10wt% of rock dust). The plate and tube shaped specimens were formed under the pressure of 1ton/㎠. The specimens were bloated at 1150℃ for 1 hour in the atmospheric ambient. The bloated porous ceramics have the characteristics-bulk density 0.6∼0.8g/㎤, compressive strength 77∼200kg/㎠ and thermal conductivity 0.58W/mK. Above the characteristics show that the bloated porous ceramics can be used in the field of refractory materials and light-weight constructional materials.

Consistency of seasonal patterns of insect succession on pig cadavers at Yeongdo Island in Busan, Korea

Park Sang‐Hyun,Moon Tae‐Young 한국곤충학회 2021 Entomological Research Vol.51 No.1

Cadaver insects cause dead bodies to break down in ecological processes. Still, the knowledge has been accumulated slowly about the structure and role of the insect assemblages attending to death scenes. This study analysed the successional patterns of insects on pig cadavers in the spring, summer, and autumn from 2016 to 2019 at Yeongdo Island, Busan, South Korea. A sum of 71 insect species belonging to four orders and 21 families were sampled. Predominant insects were largely Diptera and Coleoptera. The majority of the flies were the calliphorids as Chrysomya pinguis (Walker) and Lucilia porphyrina (Walker). The most common beetles were Creophilus maxillosus (L.), Omosita japonica (Reitter), and Staphylinidae sp. regardless of seasons. Occurrence matrices were made from the successional patterns of insect species during 42 sampling intervals in the spring, 38 sampling intervals in the summer, and 26 sampling intervals in the autumn. Permutation analyses of the occurrence matrices showed that the patterns of succession were similar between spring 2016 and 2017 (P = 0.019), between summer 2016 and 2017 (P = 0.003), and between autumn 2016 and 2019 (P = 0.002). The result shows that there are clear patterns in succession between seasons, and provides the reference data to presume the death time, at least in the southeast part of South Korea.

Development of Evaluation Norm for Physical Fitness On Sasang Constitutions in Female College Students

( Sang-hyun Kim ),( Sung-eun You ),( Tae-yeong Kim ),( You-sun Ko ) 한국체육학회 2016 국제스포츠과학 학술대회 Vol.2016 No.1

Purpose: This research aims to develop the evaluation norm of physical fitness of women college students based on Sasang constitutional types and with these, to provide materials necessary to tailored exercise programs by individual differences and develop physical education programs for improving physical competence. Method: From 2008 to 2015, targeted students of S Women’s university located in Seoul Korea, total 1,263 answered questionnaires and were measured their strength. The data were classified by the Sasang constitution analysis program (QSCCⅡ): So-Yang type n=449, So-Eum type n=542, Tae-Eum type n=272. Measurement variables of Physical fitness are Muscular strength(grip strength, back strength), Muscular endurance(sit-up), Power(standing long jump), Agility(tapping), Flexibility(trunk flexion). For data analysis, with SPSS 18.0 technological statistics and One-way ANOVA were carried out, adapting 5-level by Cajori. Result: 1. The physical fitness has significant differences among Sasang constitutional types: Tae-Eum type has best muscular strength and So-Yang type had the highest Power. 2. By comparing evaluation norm of physical fitness of total women students with those of physical fitness on Sasang constitutional types showed different figures< Table. 1 > Conclusion: This research implicates that the evaluation norm of physical fitness on Sasang constitutional types may provide more detailed and accurate one. Furthermore, by presenting reliable workout programs, more healthy and satisfactory results-improvement of exercise competence-can be acquired. Future researches will contribute to develop customized workout programs on Sasang constitutional types for not only women students but people of all ages.

The impact of blowflies on pig cadaver decomposition on Yeongdo Island, Busan, South Korea

Park Sang‐Hyun,Baek Seung‐Ho,Moon Tae‐Young 한국곤충학회 2021 Entomological Research Vol.51 No.11

The decomposition process is an important factor in forensics, and flies play a crucial role in the decomposition of animals and humans. However, there remains limited information on the effect of flies that attend death scenes. This study assesses the effect of the presence or absence of blowflies on the rate and process of cadaver decomposition in the spring, summer, and autumn from 2016 to 2020 on Yeongdo Island, Busan, South Korea. Nine blowfly species belonging to four genera in two subfamilies were sampled. The blowflies that approached the cadavers differed between seasons, but Chrysomya pinguis (Walker) was the dominant species regardless of the season. Blowfly presence on cadavers significantly affected the duration of the decomposition stage and the pattern of weight loss in nearly all scenarios. With the presence of blowflies, the decomposition rate was four times faster in spring, three times faster in summer, and nine times faster in autumn. These results show that the presence or absence of blowflies is an essential factor in the decomposition process of cadavers. Therefore, it should be taken into account when estimating postmortem intervals in medicolegal forensic entomology.

Forensic implication of cadaveric behaviors of the Japanese pavement ant (Tetramorium tsushimae Emery) attending to rabbit cadavers

Park Sang‐Hyun,Moon Tae‐Young 한국곤충학회 2020 Entomological Research Vol.50 No.4

It is noticed by some researchers that ants may cause some forensic confusion in understanding death scenes. In the aspect of relationship between the ant-animal decomposition, we observed the behavior of ants congregating on dead animals. Amongst various species of ants visiting dead rabbits, Tetramorium tsushimae Emery was noticeable in producing scratched scars, mounds, and nests around dead rabbits and covering them with soils. The behaviors are likely to interfere or to disturb the usual process of decomposition succession lead by maggots. We discussed that a series of cadaveric behaviors of T. tsushimae may be used as a potential forensic hint not to misunderstand death scenes disturbed by the species.

비수리(Lespedeza cuneata G. Don) 메탄올 추출물로부터 분획된 용매분획물의 항산화활성과 α-Glucosidase 저해활성

김현영(Hyun Young Kim),고지연(Jee Yeon Ko),송석보(Seuk Bo Song),김정인(Jung In Kim),서혜인(Hye In Seo),이재생(Jae Saeng Lee),곽도연(Do Yeon Kwak),정태욱(Tae Wook Jung),김기영(Ki Young Kim),오인석(In Seok Oh),정헌상(Heon Sang Jeong),우 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11

비수리의 식품학적 이용가능성을 확인해 보고자 항산화성분 및 항산화활성, superoxide dismutase 유사활성, α-glucosidase 저해활성 등에 대해 검토하였다. 비수리 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획 등 순차적 용매분획물의 총 폴리페놀 함량은 각각 12.44, 3.61, 6.39, 27.11, 20.00 및 9.32 ㎎ GAE/g extract residue(ER)로 나타났으며, 총 플라보노이드 함량은 각각 2.94, 9.92, 7.77, 9.27, 5.11 및 2.66 ㎎ CE/g ER, 총 탄닌 함량은 각각 8.75, 10.04, 7.42, 17.32, 11.65 및 7.61 ㎎ TAE/g ER, 총 프로안토시아니딘의 함량은 346.09, 63.50, 103.76, 288.62, 231.99 및 358.48 ㎍ CE/g ER로 나타났다. 비수리 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획물의 DPPH radical 소거활성은 각각 20.62, 5.16, 9.29, 20.80, 20.00 및 20.79 ㎎ TE/g ER, ABTS radical 소거활성은 각각 33.86, 9.24, 17.36, 33.76, 33.49 및 33.86 ㎎ TE/g ER로 나타났다. SOD 유사활성은 각각 4.12, 0.61, 2.01, 9.89, 13.47 및 11.82 unit/mL의 활성을 보여 butanol 분획과 물 분획에서 유의적으로 높은 활성을 나타내었고 α-glucosidase 저해활성은 물 분획 50 및 25 ㎍/mL에서 각각 93.85 및 61.64%로 높은 저해활성을 보이는 것으로 나타났다. 이상의 결과에서 비수리 추출물은 항산화성분 및 항산화활성, SOD 유사활성, α-glucosidase 저해활성을 가지는 물질을 함유한 것으로 보이며, 체내 및 식품에서 활성산소 종 제거에 도움을 줄 수 있을 것으로 보인다. The purpose of this study was to evaluate the antioxidant contents and antioxidant activities of solvent fractions from methanolic extract of sericea lespedeza. To determine the antioxidant compounds in solvent fractions from methanolic extract, total polyphenolic, flavonoid, tannin, and proanthocyanidin contents were measured by spectrophotometric methods. Solvent fractions were evaluated for antioxidative capacity according to DPPH and ABTS radical scavenging activities. Total polyphenolic contents were 12.44, 3.61, 6.39, 27.11, 20.00, and 9.32 ㎎ gallic acid equivalent (GAE)/g extract residue (ER), respectively. Total flavonoid contents were 2.94, 9.92, 7.77, 9.27, 5.11, and 2.66 ㎎ catechin equivalent (CE)/g ER, respectively. Total tannin contents were 8.75, 10.04, 7.42, 17.32, 11.65, and 7.61 ㎎ tannic acid equivalent (TAE)/g ER, respectively. Total proanthocyanidin contents were 346.09, 63.50, 103.76, 288.62, 231.99, and 358.48 ㎍ CE/g ER, respectively. DPPH radical scavenging activities of solvent fractions from methanolic extract of sericea lespedeza were 20.62, 5.16, 9.29, 20.80, 20.00 and 20.79 ㎎ Trolox equivalent (TE)/g ER, and ABTS radical scavenging activities were 33.86, 9.24, 17.36, 33.76, 33.49, and 33.86 ㎎ TE/g ER, respectively. SOD-like activities were 4.12, 0.61, 2.01, 9.89, 13.47, and 11.82 units/mL, and α-glucosidase inhibition activities were 93.85 and 61.64% at concentrations of 50 and 25 ㎍/mL in the water fraction, respectively. The results of this study show that notable antioxidant activities in sericea lespedeza have significant health benefits.

맨드라미(Celosia cristata L.) 꽃 메탄올 추출물로부터 용매분획된 분획물의 항산화활성

김현영(Hyun Young Kim),고지연(Jee Yeon Ko),송석보(Seuk Bo Song),김정인(Jung In Kim),서혜인(Hye In Seo),이재생(Jae Saeng Lee),곽도연(Do Yeon Kwak),정태욱(Tae Wook Jung),김기영(Ki Young Kim),오인석(In Seok Oh),정헌상(Heon Sang Jeong),우 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11

맨드라미꽃의 식품학적 이용가능성을 확인해 보고자 항산화성분 및 항산화활성에 대해 검토하였다. 맨드라미꽃의 항산화성분과 활성은 메탄올 추출물과 순차적 용매분획물에 대해 측정하였으며, 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획물의 추출수율은 각각 23.33, 10.27, 20.00, 13.63, 17.55 및 38.54%였다. 맨드라미 꽃 메탄올 추출물의 총 polyphenol, flavonoid 및 tannin 함량은 각각 6.80 ㎎ GAE/g ME, 2.34 ㎎ CE/g ME 및 6.23 ㎎ TAE/g ME였다. 순차적 용매분획물 중 butanol 분획에서 각각 14.92 ㎎ GAE/g SF, 5.44 ㎎ CE/g SF 및 13.38 ㎎ TAE/g SF로 함량이 높았다. 총 proanthocyanidin 함량은 44.72 ㎍ CE/g ME였고 순차적 용매분획물은 각각 42.47, 44.43, 50.03, 49.12 및 41.80 ㎍ CE/g ER로 용매간 유의적인 차이를 보이지 않았다. 맨드라미꽃의 메탄올 추출물의 DPPH 및 ABTS radical 소거활성은 각각 5.24 및 10.70 ㎎ TE/g ME였고 butanol 분획에서 각각 12.53 및 21.09 ㎎ TE/g SF로 활성이 높았다. 메탄올 추출물의 SOD 유사활성은 7.96 unit/mL로 나타났고 hexane, chloroform, ethyl acetate, butanol 및 물 분획물은 각각 4.56, 6.15, 8.07, 12.36 및 5.21 unit/mL로 butanol 분획의 활성이 높았다. 맨드라미꽃 추출물의 항산화성분과 항산화활성 간에 높은 정의 상관관계를 보였고 맨드라미꽃 추출물이 체내 및 식품에서 유익한 도움을 줄 수 있을 것으로 생각된다. The purpose of this study was to evaluate the antioxidant contents and activities of solvent fractions from methanolic extracts of cockscome flowers. The yield of methanolic extracts from cockscome flowers was 23.33%, whereas those of its solvent fractions (hexane, chloroform, ethyl acetate, butanol, and water) were 10.27, 20.00, 13.63, 17.55, and 38.54%, respectively. Total polyphenolic, flavonoid, tannin, and proanthocyanidin contents of methanolic extracts (ME) were 6.80 ㎎ gallic acid equivalent (GAE)/g ME, 2.34 ㎎ catechin equivalent (CE)/g ME, 6.23 ㎎ tannic acid equivalent (TAE)/g ME, and 44.72 ㎍ CE/g ME, respectively. The highest total polyphenolic, flavonoid, and tannin contents of solvent fractions were 14.92 ㎎ GAE/g solvent fraction (SF), 5.44 ㎎ CE/g SF, and 13.38 ㎎ TAE/g SF in the butanol fraction, respectively. The total proanthocyanidin contents were 42.47, 44.43, 50.03, 49.12, and 41.80 ㎍ CE/g SF, respectively. The DPPH and ABTS radical scavenging activities from cockscome flowers were 5.24 and 10.70 ㎎ Trolox equivalent (TE)/g ME, respectively. The highest DPPH and ABTS radical scavenging activities of the solvent fractions were 12.53 and 21.09 ㎎ TE/g SF in the butanol fraction, respectively. SOD-like activities of methanolic extracts from cockscome flowers were 7.96 units/mL, whereas those of its solvent fractions were 4.56, 6.15, 8.07, 12.36, and 5.21 units/mL, respectively. The results of this study show that notable antioxidant activities in cockscome flowers have significant health benefits.

SWAT 모형을 이용한 태화강유역 장기유출 분석

신현석(Shin Hyun Suk),강두기(Kang Du Kee),김균태(Kim Kyun Tae) 대한토목학회 2004 대한토목학회 학술대회 Vol.2004 No.10

임신부 뇨로부터 정제된 인간 상피세포 증식 인자 유사체의 in vitro bioassay 및 특성

박세철,전재현,남정현,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

벤조산흡착, 음이온 교환수지, 단일클론항체을 이용한 immunoaffinity chromatography를 통하여 임신부 뇨로부터 천연의 hEGF를 정제하였다. 정제된 hEGF는 μ Bonda C_18 column을 사용한 HPLC 분석을 통하여 4개의 fraction으로 분리가 가능하였으며 western blot과 double immunodiffusion 실험 결과, 각각의 fraction이 hEGF의 특성을 가진 유사체인 것을 알 수 있었다. 또한 hEGF 표준 물질과의 spiking 및 아미노산 분석 등을 통하여 두 번째 fraction이 nhEGF와 동일한 것으로 확인하였다. nhEGF 및 그 유사체의 생물학적 활성 비교를 위하여 NIH 3T3 세포주에서 5'-Brdu incorporation 측정을 위한 labelling 시간, 혈청 농도의 최적 조건을 결정하였다. NIH 3T3 세포주의 DNA 합성능은 0.2% FCS가 포함된 저혈청 배지에서 hEGF가 0.1~10 ng/ml 농도로 첨가하였을 때 증가하는 경향을 나타냈다. HPLC를 통하여 분리된 두번째 hEGF 유사체가 다른 유사체보다도 생물학적인 활성이 우수하였으며, rhEGF 표준물질과의 spiking 및 아미노산 서열 분석등을 통하여 nhEGF로 밝혀졌다. 임신부의 뇨의 hEGF 유사체 함량중 natural hEGF는 46%이었다. Natural human epidermal growth facto (nhEGF) was purified from pregnant human urine by benzoic acid adsorption, DEAE-Sepharose ion exchange, and immunoaffinity chromatography. The purified nhEGF was further separated into four fractions using Bondapak C_18 HPLC system. Following characterization by Western blot and double immunodiffusion, we found that each fraction corresponds to four derivatives of the nhEGF. For biological analysis of nhEGF, we optimized the labeling time and serum concentration for the incorporation of 5-bromo-2'-deoxy-uridine (BrdU), a non-radioactive alternative for [^3H]-thymidine uptake, into NIH 3T3 cells. The DNA synthesis of NIH 3T3 cells was gradually increased at the nhEGF concentrations between 0.1~10 ng/ml in the Dulbecco's Modified Eagles Medium (DMEM) containing 0.2% Fetal calf serum (FCS). When we assayed the biological activity of four fractions, the activity of the second fraction was superior to that of the others.